Spanevello_2008_Neurochem.Res_33_1129

Reference

Title : Effects in vitro of guanidinoacetate on adenine nucleotide hydrolysis and acetylcholinesterase activity in tissues from adult rats - Spanevello_2008_Neurochem.Res_33_1129
Author(s) : Spanevello RM , de Souza Wyse AT , Mazzanti CM , Schmatz R , Stefanello N , Goncalves JF , Bagatini M , Battisti V , Morsch VM , Schetinger MR
Ref : Neurochem Res , 33 :1129 , 2008
Abstract :

Guanidinoacetate methyltransferase (GAMT) deficiency is a disorder of creatine metabolism characterized by low plasma creatine concentrations in combination with elevated guanidinoacetate (GAA) concentrations. The aim of this work was to investigate the in vitro effect of guanidinoacetate in NTPDase, 5'-nucleotidase and acetylcholinesterase activities in the synaptosomes, platelets and blood of rats. The results showed that in synaptosomes the NTPDase and 5'-nucleotidase activities were inhibited significantly in the presence of GAA at concentrations of 50, 100, 150 and 200 microM (P < 0.05). However, in platelets GAA at the same concentrations caused a significant increase in the activities of these two enzymes (P < 0.05). In relation to the acetylcholinesterase activity, GAA caused a significant inhibition in the activity of this enzyme in blood at concentrations of 150 and 200 microM (P < 0.05), but did not alter the acetylcholinesterase activity in synaptosomes from the cerebral cortex. Our results suggest that alterations caused by GAA in the activities of these enzymes may contribute to the understanding of the neurological dysfunction of GAMT-deficient patients.

PubMedSearch : Spanevello_2008_Neurochem.Res_33_1129
PubMedID: 18256932

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Citations formats

Spanevello RM, de Souza Wyse AT, Mazzanti CM, Schmatz R, Stefanello N, Goncalves JF, Bagatini M, Battisti V, Morsch VM, Schetinger MR (2008)
Effects in vitro of guanidinoacetate on adenine nucleotide hydrolysis and acetylcholinesterase activity in tissues from adult rats
Neurochem Res 33 :1129

Spanevello RM, de Souza Wyse AT, Mazzanti CM, Schmatz R, Stefanello N, Goncalves JF, Bagatini M, Battisti V, Morsch VM, Schetinger MR (2008)
Neurochem Res 33 :1129