Speck_2023_Cell.Rep_42_113389

Reference

Title : Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria - Speck_2023_Cell.Rep_42_113389
Author(s) : Speck SL , Bhatt DP , Zhang Q , Adak S , Yin L , Dong G , Feng C , Zhang W , Ben Major M , Wei X , Semenkovich CF
Ref : Cell Rep , 42 :113389 , 2023
Abstract :

Acyl-protein thioesterases 1 and 2 (APT1 and APT2) reverse S-acylation, a potential regulator of systemic glucose metabolism in mammals. Palmitoylation proteomics in liver-specific knockout mice shows that APT1 predominates over APT2, primarily depalmitoylating mitochondrial proteins, including proteins linked to glutamine metabolism. miniTurbo-facilitated determination of the protein-protein proximity network of APT1 and APT2 in HepG2 cells reveals APT proximity networks encompassing mitochondrial proteins including the major translocases Tomm20 and Timm44. APT1 also interacts with Slc1a5 (ASCT2), the only glutamine transporter known to localize to mitochondria. High-fat-diet-fed male mice with dual (but not single) hepatic deletion of APT1 and APT2 have insulin resistance, fasting hyperglycemia, increased glutamine-driven gluconeogenesis, and decreased liver mass. These data suggest that APT1 and APT2 regulation of hepatic glucose metabolism and insulin signaling is functionally redundant. Identification of substrates and protein-protein proximity networks for APT1 and APT2 establishes a framework for defining mechanisms underlying metabolic disease.

PubMedSearch : Speck_2023_Cell.Rep_42_113389
PubMedID: 37925639
Gene_locus related to this paper: human-LYPLA1 , human-LYPLA2 , mouse-lypla1 , mouse-lypla2

Related information

Gene_locus human-LYPLA1    human-LYPLA2    mouse-lypla1    mouse-lypla2

Citations formats

Speck SL, Bhatt DP, Zhang Q, Adak S, Yin L, Dong G, Feng C, Zhang W, Ben Major M, Wei X, Semenkovich CF (2023)
Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria
Cell Rep 42 :113389

Speck SL, Bhatt DP, Zhang Q, Adak S, Yin L, Dong G, Feng C, Zhang W, Ben Major M, Wei X, Semenkovich CF (2023)
Cell Rep 42 :113389