Title : Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17 - Tratsiak_2013_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_69_683 |
Author(s) : Tratsiak K , Degtjarik O , Drienovska I , Chrast L , Rezacova P , Kuty M , Chaloupkova R , Damborsky J , Kuta Smatanova I |
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 69 :683 , 2013 |
Abstract :
Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 A. |
PubMedSearch : Tratsiak_2013_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_69_683 |
PubMedID: 23722854 |
Gene_locus related to this paper: 9alte-a3jb27 , psyck-q1qbb9 |
Substrate | 1-bromohexane 1,2-dichloroethane |
Gene_locus | 9alte-a3jb27 psyck-q1qbb9 |
Structure | 6F9O 5MXP |
Tratsiak K, Degtjarik O, Drienovska I, Chrast L, Rezacova P, Kuty M, Chaloupkova R, Damborsky J, Kuta Smatanova I (2013)
Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17
Acta Crystallographica Sect F Struct Biol Cryst Commun
69 :683
Tratsiak K, Degtjarik O, Drienovska I, Chrast L, Rezacova P, Kuty M, Chaloupkova R, Damborsky J, Kuta Smatanova I (2013)
Acta Crystallographica Sect F Struct Biol Cryst Commun
69 :683