Tratsiak_2013_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_69_683

Reference

Title : Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17 - Tratsiak_2013_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_69_683
Author(s) : Tratsiak K , Degtjarik O , Drienovska I , Chrast L , Rezacova P , Kuty M , Chaloupkova R , Damborsky J , Kuta Smatanova I
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 69 :683 , 2013
Abstract :

Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 A.

PubMedSearch : Tratsiak_2013_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_69_683
PubMedID: 23722854
Gene_locus related to this paper: 9alte-a3jb27 , psyck-q1qbb9

Related information

Substrate 1-bromohexane    1,2-dichloroethane
Gene_locus 1-bromohexane    1,2-dichloroethane    9alte-a3jb27    psyck-q1qbb9
Structure 1-bromohexane    1,2-dichloroethane    9alte-a3jb27    psyck-q1qbb9    6F9O    5MXP

Citations formats

Tratsiak K, Degtjarik O, Drienovska I, Chrast L, Rezacova P, Kuty M, Chaloupkova R, Damborsky J, Kuta Smatanova I (2013)
Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17
Acta Crystallographica Sect F Struct Biol Cryst Commun 69 :683

Tratsiak K, Degtjarik O, Drienovska I, Chrast L, Rezacova P, Kuty M, Chaloupkova R, Damborsky J, Kuta Smatanova I (2013)
Acta Crystallographica Sect F Struct Biol Cryst Commun 69 :683