Uno_2014_Drug.Metab.Dispos_42_2002

Reference

Title : Systematic identification and characterization of carboxylesterases in cynomolgus macaques - Uno_2014_Drug.Metab.Dispos_42_2002
Author(s) : Uno Y , Uehara S , Hosokawa M , Imai T
Ref : Drug Metabolism & Disposition: The Biological Fate of Chemicals , 42 :2002 , 2014
Abstract :

Carboxylesterase (CES) is important for detoxification of a wide range of drugs and xenobiotics and catalyzes cholesterol and fatty acid metabolism. Cynomolgus macaques are widely used in drug metabolism studies; however, cynomolgus CES has not been fully investigated at molecular levels, partly due to the lack of gene information. In this study, we isolated and characterized cDNAs for CES homologous to human CES1, CES2, and CES5A in cynomolgus macaques. By genome analysis, in the cynomolgus macaque genome, three gene sequences were found for CES1(v1-3) and CES2(v1-3), whereas one gene sequence was found for CES5A. Cynomolgus CES1, CES2, and CES5A genes were located in the genomic regions corresponding to the human genes. We successfully identified CES1v1, CES1v2, CES2v1, CES2v3, and CES5A cDNAs from cynomolgus liver. Sequence analysis showed that amino acid sequences of each CES were highly homologous to that of the human homolog. All five CESs had sequences characteristic for CES enzymes, including the catalytic triad and oxyanion hole loop. By quantitative polymerase chain reaction, the most abundant expression of CES mRNAs among the 10 tissue types analyzed was observed in liver (CES1v1 and CES2v3 mRNAs), jejunum (CES2v1 mRNAs), and kidney (CES1v2 and CES5A mRNA), the organs important for drug metabolism and excretion. The results indicated that cynomolgus macaques express at least five CES genes, which potentially encode intact CES proteins.

PubMedSearch : Uno_2014_Drug.Metab.Dispos_42_2002
PubMedID: 25256558
Gene_locus related to this paper: macfa-CES1v2 , macfa-CES1v1 , macfa-CES5 , macfa-CES2v3

Related information

Gene_locus macfa-CES1v2    macfa-CES1v1    macfa-CES5    macfa-CES2v3
Gene_locus_frgt macfa-a0a171p0z6

Citations formats

Uno Y, Uehara S, Hosokawa M, Imai T (2014)
Systematic identification and characterization of carboxylesterases in cynomolgus macaques
Drug Metabolism & Disposition: The Biological Fate of Chemicals 42 :2002

Uno Y, Uehara S, Hosokawa M, Imai T (2014)
Drug Metabolism & Disposition: The Biological Fate of Chemicals 42 :2002