Wang_2015_PLoS.One_10_e0123986

Reference

Title : Organelle-Specific Nitric Oxide Detection in Living Cells via HaloTag Protein Labeling - Wang_2015_PLoS.One_10_e0123986
Author(s) : Wang J , Zhao Y , Wang C , Zhu Q , Du Z , Hu A , Yang Y
Ref : PLoS ONE , 10 :e0123986 , 2015
Abstract :

Nitric oxide (NO) is a membrane-permeable signaling molecule that is constantly produced, transferred, and consumed in vivo. NO participates and plays important roles in multiple biological processes. However, spatiotemporal imaging of NO in living cells is challenging. To fill the gap in currently used techniques, we exploited the versatility of HaloTag technology and synthesized a novel organelle-targetable fluorescent probe called HTDAF-2DA. We demonstrate the utility of the probe by monitoring subcellular NO dynamics. The developed strategy enables precise determination of local NO function.

PubMedSearch : Wang_2015_PLoS.One_10_e0123986
PubMedID: 25923693

Related information

Citations formats

Wang J, Zhao Y, Wang C, Zhu Q, Du Z, Hu A, Yang Y (2015)
Organelle-Specific Nitric Oxide Detection in Living Cells via HaloTag Protein Labeling
PLoS ONE 10 :e0123986

Wang J, Zhao Y, Wang C, Zhu Q, Du Z, Hu A, Yang Y (2015)
PLoS ONE 10 :e0123986