| Title : Investigating the Function of Cryptic Cytochalasan Cytochrome P450 Monooxygenases Using Combinatorial Biosynthesis - Wang_2019_Org.Lett_21_8756 |
| Author(s) : Wang C , Becker K , Pfutze S , Kuhnert E , Stadler M , Cox RJ , Skellam E |
| Ref : Org Lett , 21 :8756 , 2019 |
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Abstract :
Tailoring enzymes in cytochalasan biosynthesis are relatively promiscuous. Exploiting this property, we deduced the function of four cryptic cytochrome P450 monooxygenases via heterologous expression of six cytochrome P450-encoding genes, originating from Hypoxylon fragiforme and Pyricularia oryzae, in pyrichalasin H deltaP450 strains. Three cryptic cytochrome P450 enzymes (HffD, HffG, and CYP1) restored pyrichalasin H production in mutant strains, while CYP3 catalyzed a site-selective epoxidation leading to the isolation of three novel cytochalasans. |
| PubMedSearch : Wang_2019_Org.Lett_21_8756 |
| PubMedID: 31644300 |
| Gene_locus related to this paper: phano-phmG , aspcl-CCSE , aspfu-psoB , mago7-ORFZB , maggr-pyie |
| Gene_locus | phano-phmG aspcl-CCSE aspfu-psoB mago7-ORFZB maggr-pyie |
Wang C, Becker K, Pfutze S, Kuhnert E, Stadler M, Cox RJ, Skellam E (2019)
Investigating the Function of Cryptic Cytochalasan Cytochrome P450 Monooxygenases Using Combinatorial Biosynthesis
Org Lett
21 :8756
Wang C, Becker K, Pfutze S, Kuhnert E, Stadler M, Cox RJ, Skellam E (2019)
Org Lett
21 :8756