Wu_2016_Environ.Pollut_213_793

Reference

Title : Microcystin-LR exposure induces developmental neurotoxicity in zebrafish embryo - Wu_2016_Environ.Pollut_213_793
Author(s) : Wu Q , Yan W , Liu C , Li L , Yu L , Zhao S , Li G
Ref : Environ Pollut , 213 :793 , 2016
Abstract :

Microcystin-LR (MCLR) is a commonly acting potent hepatotoxin and has been pointed out of potentially causing developmental neurotoxicity, but the exact mechanism is little known. In this study, zebrafish embryos were exposed to 0, 0.8, 1.6 or 3.2 mg/L MCLR for 120 h. MCLR exposure through submersion caused serious hatching delay and body length decrease. The content of MCLR in zebrafish larvae was analyzed and the results demonstrated that MCLR can accumulate in zebrafish larvae. The locomotor speed of zebrafish larvae was decreased. Furthermore, the dopamine and acetylcholine (ACh) content were detected to be significantly decreased in MCLR exposure groups. And the acetylcholinesterase (AChE) activity was significantly increased after exposure to 1.6 and 3.2 mg/L MCLR. The transcription pattern of manf, chrnalpha7 and ache gene was consistent with the change of the dopamine content, ACh content and AChE activity. Gene expression involved in the development of neurons was also measured. a1-tubulin and shha gene expression were down-regulated, whereas mbp and gap43 gene expression were observed to be significantly up-regulated upon exposure to MCLR. The above results indicated that MCLR-induced developmental toxicity might attribute to the disorder of cholinergic system, dopaminergic signaling, and the development of neurons.

PubMedSearch : Wu_2016_Environ.Pollut_213_793
PubMedID: 27038211

Related information

Citations formats

Wu Q, Yan W, Liu C, Li L, Yu L, Zhao S, Li G (2016)
Microcystin-LR exposure induces developmental neurotoxicity in zebrafish embryo
Environ Pollut 213 :793

Wu Q, Yan W, Liu C, Li L, Yu L, Zhao S, Li G (2016)
Environ Pollut 213 :793