Li G

References (120)

Title : Pharmacokinetics, Pharmacodynamics, and Safety of Single Dose HSK7653 Tablets in Chinese Subjects with Normal or Impaired Renal Function - Shi_2024_Clin.Pharmacokinet__
Author(s) : Shi D , Chen L , Li G , Wu N , Zhang F , Wang X , Mu N , Chen X , Yang X , Lu J , Lu Y , Wang M , Zhang D
Ref : Clinical Pharmacokinetics , : , 2024
Abstract : OBJECTIVE: HSK7653 is a novel, ultralong-acting dipeptidyl peptidase-4 (DPP-4) inhibitor, promising for type 2 diabetes mellitus with a dosing regimen of once every 2 weeks. This trial investigates the pharmacokinetics (PKs), pharmacodynamics (PDs),and safety of HSK7653 in outpatients with normal or impaired renal function. METHODS: This is a multicenter, open-label, nonrandomized, parallel-controlled phase I clinical study that investigates the pharmacokinetic profiles of HSK7653 after a single oral administration in 42 subjects with mild (n = 8), moderate (n = 10), severe renal impairment (n = 10), and end-stage renal disease (without dialysis, n = 5) compared with matched control subjects with normal renal function (n = 9). Safety was evaluated throughout the study, and the pharmacodynamic effects were assessed on the basis of a DPP-4 inhibition rate. RESULTS: HSK7653 exposure levels including the maximum plasma concentration (C(max)), area under the plasma concentration-time curve from zero to last time of quantifiable concentration (AUC(0-t)), and area under the plasma concentration-time curve from zero to infinity (AUC(0-inf)) showed no significant differences related to the severity of renal impairment. Renal clearance (CL(R)) showed a certain downtrend along with the severity of renal impairment. The CL(R) of the group with severe renal impairment and the group with end-stage renal disease were basically similar. The DPP-4 inhibition rate-time curve graph was similar among the renal function groups. All groups had favorable safety, and no serious adverse events occurred. CONCLUSIONS: HSK7653 is a potent oral DPP-4 inhibitor with a long plasma half-life, supporting a dosing regimen of once every 2 weeks. Impaired renal function does not appear to impact the pharmacokinetic and pharmacodynamic properties of HSK7653 after a single administration in Chinese subjects. HSK7653 is also well tolerated without an increase in adverse events with increasing renal impairment. These results indicate that dose adjustment of HSK7653 may not be required in patients with renal impairment. TRIAL REGISTRATION: ClinicalTrials.gov Identifier: NCT05497297.
ESTHER : Shi_2024_Clin.Pharmacokinet__
PubMedSearch : Shi_2024_Clin.Pharmacokinet__
PubMedID: 38184489

Title : Improvement of plant resistance to geminiviruses via protein de-S-acylation - Zhao_2024_Stress.Biol_4_23
Author(s) : Zhao Y , Li Z , Wang Z , Huang L , Li G , Liu X , Yuan M , Huang W , Ling L , Yang C , He Z , Lai J
Ref : Stress Biol , 4 :23 , 2024
Abstract : Geminiviruses are an important group of viruses that infect a variety of plants and result in heavy agricultural losses worldwide. The homologs of C4 (or L4) in monopartite geminiviruses and AC4 (or AL4) in bipartite geminiviruses are critical viral proteins. The C4 proteins from several geminiviruses are the substrates of S-acylation, a dynamic post-translational modification, for the maintenance of their membrane localization and function in virus infection. Here we initiated a screening and identified a plant protein ABAPT3 (Alpha/Beta Hydrolase Domain-containing Protein 17-like Acyl Protein Thioesterase 3) as the de-S-acylation enzyme of C4 encoded by BSCTV (Beet severe curly top virus). Overexpression of ABAPT3 reduced the S-acylation of BSCTV C4, disrupted its plasma membrane localization, inhibited its function in pathogenesis, and suppressed BSCTV infection. Because the S-acylation motifs are conserved among C4 from different geminiviruses, we tested the effect of ABAPT3 on the C4 protein of ToLCGdV (Tomato leaf curl Guangdong virus) from another geminivirus genus. Consistently, ABAPT3 overexpression also disrupted the S-acylation, subcellular localization, and function of ToLCGdV C4, and inhibited ToLCGdV infection. In summary, we provided a new approach to globally improve the resistance to different types of geminiviruses in plants via de-S-acylation of the viral C4 proteins and it can be extendedly used for suppression of geminivirus infection in crops.
ESTHER : Zhao_2024_Stress.Biol_4_23
PubMedSearch : Zhao_2024_Stress.Biol_4_23
PubMedID: 38662136
Gene_locus related to this paper: arath-At5g14390

Title : Significant but partial lipoprotein lipase functional loss caused by a novel occurrence of rare LPL biallelic variants - Hu_2024_Lipids.Health.Dis_23_92
Author(s) : Hu Y , Chen JM , Zuo H , Pu N , Zhang G , Duan Y , Li G , Tong Z , Li W , Li B , Yang Q
Ref : Lipids Health Dis , 23 :92 , 2024
Abstract : BACKGROUND: Lipoprotein lipase (LPL) plays a crucial role in triglyceride hydrolysis. Rare biallelic variants in the LPL gene leading to complete or near-complete loss of function cause autosomal recessive familial chylomicronemia syndrome. However, rare biallelic LPL variants resulting in significant but partial loss of function are rarely documented. This study reports a novel occurrence of such rare biallelic LPL variants in a Chinese patient with hypertriglyceridemia-induced acute pancreatitis (HTG-AP) during pregnancy and provides an in-depth functional characterization. METHODS: The complete coding sequences and adjacent intronic regions of the LPL, APOC2, APOA5, LMF1, and GPIHBP1 genes were analyzed by Sanger sequencing. The aim was to identify rare variants, including nonsense, frameshift, missense, small in-frame deletions or insertions, and canonical splice site mutations. The functional impact of identified LPL missense variants on protein expression, secretion, and activity was assessed in HEK293T cells through single and co-transfection experiments, with and without heparin treatment. RESULTS: Two rare LPL missense variants were identified in the patient: the previously reported c.809G > A (p.Arg270His) and a novel c.331G > C (p.Val111Leu). Genetic testing confirmed these variants were inherited biallelically. Functional analysis showed that the p.Arg270His variant resulted in a near-complete loss of LPL function due to effects on protein synthesis/stability, secretion, and enzymatic activity. In contrast, the p.Val111Leu variant retained approximately 32.3% of wild-type activity, without impacting protein synthesis, stability, or secretion. Co-transfection experiments indicated a combined activity level of 20.7%, suggesting no dominant negative interaction between the variants. The patient's post-heparin plasma LPL activity was about 35% of control levels. CONCLUSIONS: This study presents a novel case of partial but significant loss-of-function biallelic LPL variants in a patient with HTG-AP during pregnancy. Our findings enhance the understanding of the nuanced relationship between LPL genotypes and clinical phenotypes, highlighting the importance of residual LPL function in disease manifestation and severity. Additionally, our study underscores the challenges in classifying partial loss-of-function variants in classical Mendelian disease genes according to the American College of Medical Genetics and Genomics (ACMG)'s variant classification guidelines.
ESTHER : Hu_2024_Lipids.Health.Dis_23_92
PubMedSearch : Hu_2024_Lipids.Health.Dis_23_92
PubMedID: 38561841
Gene_locus related to this paper: human-LPL

Title : SlCarE054 in Spodoptera litura (Lepidoptera: Noctuidae) showed direct metabolic activity to beta-cypermethrin with stereoselectivity - Xu_2024_Bull.Entomol.Res__1
Author(s) : Xu L , Liu H , Li B , Li G , Liu R , Li D
Ref : Bull Entomol Res , :1 , 2024
Abstract : Carboxylesterases (CarEs) is an important detoxification enzyme system in phase participating in insecticides resistance. In our previous study, SlCarE054, a CarEs gene from lepidoptera class, was screened out to be upregulated in a pyrethroids and organophosphates resistant population. Its overexpression was verified in two field-collected populations of Spodoptera litura (Lepidoptera: Noctuidae) resistant to pyrethroids and organophosphates by qRT-PCR. Spatiotemporal expression results showed that SlCarE054 was highly expressed in the pupae stage and the digestive tissue midgut. To further explore its role in pyrethroids and organophosphates resistance, its metabolism activity to insecticides was determined by UPLC. Its recombinant protein showed significant metabolism activity to cyhalothrin and fenvalerate, but not to phoxim or chlorpyrifos. The metabolic activity of SlCarE054 to beta-cypermethrin showed stereoselectivity, with higher metabolic activity to -cypermethrin than the enantiomer alpha-cypermethrin. The metabolite of beta-cypermethrin was identified as 3-phenoxybenzaldehyde. Further modelling and docking analysis indicated that beta-cypermethrin, cyhalothrin and fenvalerate could bind with the catalytic triad of the 3D structure of SlCarE054. The interaction of beta-cypermethrin with SlCarE054 also showed the lowest binding energy. Our work provides evidence that SlCarE054 play roles in beta-cypermethrin resistance in S. litura.
ESTHER : Xu_2024_Bull.Entomol.Res__1
PubMedSearch : Xu_2024_Bull.Entomol.Res__1
PubMedID: 38708572
Gene_locus related to this paper: spolt-SlCarE054

Title : Axially Coordinated Gold Nanoclusters Tailoring Fe-N-C Nanozymes for Enhanced Oxidase-Like Specificity and Activity - Xie_2024_Adv.Sci.(Weinh)__e2306911
Author(s) : Xie Y , Sun F , Chang K , Li G , Song Z , Huang J , Cheng X , Zhuang G , Kuang Q
Ref : Adv Sci (Weinh) , :e2306911 , 2024
Abstract : Metal-organic frameworks (MOF) derived nitrogen-doped carbon-supported monodisperse Fe (Fe-N-C) catalysts are intensively studied, but great challenges remain in understanding the relationship between the coordination structure and the performance of Fe-N-C nanozymes. Herein, a novel nanocluster ligand-bridging strategy is proposed for constructing Fe-S(1) N(4) structures with axially coordinated S and Au nanoclusters on ZIF-8 derived Fe-N-C (labeled Au(x) /Fe-S(1) N(4) -C). The axial Au nanoclusters facilitate electron transfer to Fe active sites, utilizing the bridging ligand S as a medium, thereby enhancing the oxygen adsorption capacity of composite nanozymes. Compared to Fe-N-C, Au(x) /Fe-S(1) N(4) -C exhibits high oxidase-like specificity and activity, and holds great potential for detecting acetylcholinesterase activity with a detection limit of 5.1 microU mL(-1) , surpassing most reported nanozymes.
ESTHER : Xie_2024_Adv.Sci.(Weinh)__e2306911
PubMedSearch : Xie_2024_Adv.Sci.(Weinh)__e2306911
PubMedID: 38196300

Title : Benzofuran Derivatives from Cortex Mori Radicis and Their Cholinesterase-Inhibitory Activity - Cui_2024_Molecules_29_
Author(s) : Cui X , Huang Z , Deng S , Zhang Y , Li G , Wang L , Deng Y , Wu C
Ref : Molecules , 29 : , 2024
Abstract : The phytochemical investigation of Cortex Mori Radicis led to the isolation and identification of a new prenylated benzofuranone (1) and four ring-opening derivatives (2-5) named albaphenol A-E, as well as nigranol A (6), together with ten 2-arylbenzofuran derivatives (7-16). The characterization of the structures of the new compounds and the structural revision of nigranol A (6) were conducted using the comprehensive analysis of spectroscopic data (1D/2D NMR, HRESIMS, CD, and XRD). Compounds 1-16 were tested for their inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Compounds 1 and 4 showed weak BChE-inhibitory activity (IC(50) 45.5 and 61.0 microM); six 2-arylbenzofuran derivatives showed more-potent BChE-inhibitory activity (IC(50) 2.5-32.8 microM) than the positive control galantamine (IC(50) 35.3 microM), while being inactive or weakly inhibitory toward AChE. Cathafuran C (14) exhibited the most potent and selective inhibitory activity against BChE in a competitive manner, with a Ki value of 1.7 microM. The structure-activity relationships of the benzofuran-type stilbenes were discussed. Furthermore, molecular docking and dynamic simulations were performed to clarify the interactions of the inhibitor-enzyme complex.
ESTHER : Cui_2024_Molecules_29_
PubMedSearch : Cui_2024_Molecules_29_
PubMedID: 38257228

Title : Choline metabolism reprogramming mediates an immunosuppressive microenvironment in non-small cell lung cancer (NSCLC) by promoting tumor-associated macrophage functional polarization and endothelial cell proliferation - Xiao_2024_J.Transl.Med_22_442
Author(s) : Xiao B , Li G , Gulizeba H , Liu H , Sima X , Zhou T , Huang Y
Ref : J Transl Med , 22 :442 , 2024
Abstract : INTRODUCTION: Lung cancer is a prevalent malignancy globally, and immunotherapy has revolutionized its treatment. However, resistance to immunotherapy remains a challenge. Abnormal cholinesterase (ChE) activity and choline metabolism are associated with tumor oncogenesis, progression, and poor prognosis in multiple cancers. Yet, the precise mechanism underlying the relationship between ChE, choline metabolism and tumor immune microenvironment in lung cancer, and the response and resistance of immunotherapy still unclear. METHODS: Firstly, 277 advanced non-small cell lung cancer (NSCLC) patients receiving first-line immunotherapy in Sun Yat-sen University Cancer Center were enrolled in the study. Pretreatment and the alteration of ChE after 2 courses of immunotherapy and survival outcomes were collected. Kaplan-Meier survival and cox regression analysis were performed, and nomogram was conducted to identify the prognostic and predicted values. Secondly, choline metabolism-related genes were screened using Cox regression, and a prognostic model was constructed. Functional enrichment analysis and immune microenvironment analysis were also conducted. Lastly, to gain further insights into potential mechanisms, single-cell analysis was performed. RESULTS: Firstly, baseline high level ChE and the elevation of ChE after immunotherapy were significantly associated with better survival outcomes for advanced NSCLC. Constructed nomogram based on the significant variables from the multivariate Cox analysis performed well in discrimination and calibration. Secondly, 4 choline metabolism-related genes (MTHFD1, PDGFB, PIK3R3, CHKB) were screened and developed a risk signature that was found to be related to a poorer prognosis. Further analysis revealed that the choline metabolism-related genes signature was associated with immunosuppressive tumor microenvironment, immune escape and metabolic reprogramming. scRNA-seq showed that MTHFD1 was specifically distributed in tumor-associated macrophages (TAMs), mediating the differentiation and immunosuppressive functions of macrophages, which may potentially impact endothelial cell proliferation and tumor angiogenesis. CONCLUSION: Our study highlights the discovery of ChE as a prognostic marker in advanced NSCLC, suggesting its potential for identifying patients who may benefit from immunotherapy. Additionally, we developed a prognostic signature based on choline metabolism-related genes, revealing the correlation with the immunosuppressive microenvironment and uncovering the role of MTHFD1 in macrophage differentiation and endothelial cell proliferation, providing insights into the intricate workings of choline metabolism in NSCLC pathogenesis.
ESTHER : Xiao_2024_J.Transl.Med_22_442
PubMedSearch : Xiao_2024_J.Transl.Med_22_442
PubMedID: 38730286

Title : Cofactorless oxygenases guide anthraquinone-fused enediyne biosynthesis - Gui_2023_Nat.Chem.Biol__
Author(s) : Gui C , Kalkreuter E , Liu YC , Li G , Steele AD , Yang D , Chang C , Shen B
Ref : Nat Chemical Biology , : , 2023
Abstract : The anthraquinone-fused enediynes (AFEs) combine an anthraquinone moiety and a ten-membered enediyne core capable of generating a cytotoxic diradical species. AFE cyclization is triggered by opening the F-ring epoxide, which is also the site of the most structural diversity. Previous studies of tiancimycin A, a heavily modified AFE, have revealed a cryptic aldehyde blocking installation of the epoxide, and no unassigned oxidases could be predicted within the tnm biosynthetic gene cluster. Here we identify two consecutively acting cofactorless oxygenases derived from methyltransferase and alpha/beta-hydrolase protein folds, TnmJ and TnmK2, respectively, that are responsible for F-ring tailoring in tiancimycin biosynthesis by comparative genomics. Further biochemical and structural characterizations reveal that the electron-rich AFE anthraquinone moiety assists in catalyzing deformylation, epoxidation and oxidative ring cleavage without exogenous cofactors. These enzymes therefore fill important knowledge gaps for the biosynthesis of this class of molecules and the underappreciated family of cofactorless oxygenases.
ESTHER : Gui_2023_Nat.Chem.Biol__
PubMedSearch : Gui_2023_Nat.Chem.Biol__
PubMedID: 37945897
Gene_locus related to this paper: strsp-TnmK2

Title : Developmental Neurotoxicity of Difenoconazole in Zebrafish Embryos - Yang_2023_Toxics_11_353
Author(s) : Yang Q , Deng P , Xing D , Liu H , Shi F , Hu L , Zou X , Nie H , Zuo J , Zhuang Z , Pan M , Chen J , Li G
Ref : Toxics , 11 :353 , 2023
Abstract :
ESTHER : Yang_2023_Toxics_11_353
PubMedSearch : Yang_2023_Toxics_11_353
PubMedID: 37112580

Title : Stem cell-assisted enzyme\/prodrug therapy makes drug-resistant ovarian cancer cells vulnerable to natural killer cells through upregulation of NKG2D ligands - Li_2023_Med.Oncol_40_110
Author(s) : Li G , Nikkhoi SK , Hatefi A
Ref : Med Oncol , 40 :110 , 2023
Abstract : Cancer stem-like cells (CSCs) are believed to be responsible for cancer recurrence and metastasis. Therefore, a therapeutic approach is needed to eliminate both rapidly proliferating differentiated cancer cells and slow-growing drug-resistant CSCs. Using established ovarian cancer cells lines as well as ovarian cancer cells isolated from a patient with high-grade drug-resistant ovarian carcinoma, we demonstrate that ovarian CSCs consistently express lower levels of NKG2D ligands (MICA/B and ULBPs) on their surfaces, a mechanism by which they evade natural killer (NK) cells' surveillance. Here, we discovered that exposure of ovarian cancer (OC) cells to SN-38 followed by 5-FU not only acts synergistically to kill the OC cells, but also makes the CSCs vulnerable to NK92 cells through upregulation of NKG2D ligands. Since systemic administration of these two drugs is marred by intolerance and instability, we engineered and isolated an adipose-derived stem cell (ASC) clone, which stably expresses carboxylesterase-2 and yeast cytosine deaminase enzymes to convert irinotecan and 5-FC prodrugs into SN-38 and 5-FU cytotoxic drugs, respectively. Co-incubation of ASCs and prodrugs with drug-resistant OC cells not only led to the death of the drug-resistant OC cells but also made them significantly vulnerable to NK92 cells. This study provides proof of principle for a combined ASC-directed targeted chemotherapy with NK92-assisted immunotherapy to eradicate drug-resistant OC cells.
ESTHER : Li_2023_Med.Oncol_40_110
PubMedSearch : Li_2023_Med.Oncol_40_110
PubMedID: 36862260

Title : An ultra-sensitive photoelectrochemical sensor for chlorpyrifos detection based on a novel BiOI\/TiO(2) n-n heterojunction - Lyu_2023_Anal.Chim.Acta_1275_341579
Author(s) : Lyu R , Lei Y , Zhang C , Li G , Han R , Zou L
Ref : Anal Chim Acta , 1275 :341579 , 2023
Abstract : Due to widespread application of chlorpyrifos for controlling pests in agriculture, the continuous accumulation of chlorpyrifos residue has caused serious environmental pollution.The detection of chlorpyrifos is of great significance for humans and environment because it can arise a series of diseases by inhibiting acetylcholinesterase (AChE) activity. Photoelectrochemical sensing, as an emerging sensing technology, has great potential in the detection of chlorpyrifos. It is urgent that find a suitable photoelectric sensing strategy to effectively monitor chlorpyrifos. Herein, an n-n heterojunction was constructed by uniformly immobilizing n-type 3DBiOI, which had loose porous structure composed of numerous small and thin nanosheets, on the surface of TiO(2) with anatase/rutile (AR-TiO(2)) heterophase junction. Under light irradiation, the proposed BiOI/AR-TiO(2) n-n heterojunction exhibited excellent optical absorption characteristics and photoelectrochemical activity. Additionally, the photoelectrochemical sensing platform demonstrated excellent analytical performance in monitoring chlorpyrifos. Under optimized conditions, it showed a wide detection range of 1 pg mL(-1)- 200 ng mL(-1) and a detection limit (S/N = 3) as low as 0.24 pg mL(-1), with superior selectivity and stability. The ultra-sensitivity and great specificity for detection of chlorpyrifos can be ascribed to chelation between Bi () and C=N and P=S bonds in chlorpyrifos, which had been confirmed in this work. Meanwhile, the PEC sensor also had potential application value for monitoring chlorpyrifos in water samples, lettuce and pitaya, which the recoveries of samples ranged from 96.9% to 104.7% with a relative standard deviation (RSD) of 1.11%-5.93%. This sensor provided a novel idea for constructing heterojunctions with high photoelectric conversion efficiency and had a high application prospect for the detection of chlorpyrifos and other structural analogues.
ESTHER : Lyu_2023_Anal.Chim.Acta_1275_341579
PubMedSearch : Lyu_2023_Anal.Chim.Acta_1275_341579
PubMedID: 37524465

Title : Eco-friendly and efficient extraction of polyphenols from Ligustrum robustum by deep eutectic solvent assisted ultrasound - Qin_2023_Food.Chem_429_136828
Author(s) : Qin G , Zhang F , Ren M , Chen X , Liu C , Li G , Gao Q , Qiao L , Jiang Y , Zhu L , Guo Y , Wang G
Ref : Food Chem , 429 :136828 , 2023
Abstract : An eco-friendly and efficient extraction method using deep eutectic solvents assisted ultrasound extraction (DESs-UAE) for the polyphenols from Ligustrum robustum was developed. Among the 34 kinds of DESs prepared, tetraethyl ammonium bromide: 1,2,4-butanol (Teab: 1,2,4-But) was proved to be a suitable extraction solvent based on the extraction efficiency. The extraction parameters including temperature, water content, liquid-solid ratio were optimized with response surface methodology (RSM). Under the optimal conditions, the total phenolic content (TPC) and total flavonoid content (TFC) were 101.46 +/- 2.96 mg GAE/g DW and 264.17 +/- 5.39 mg RE/g DW, respectively. Furthermore, the extraction mechanism of DESs-UAE was investigated by extraction kinetics, molecular dynamic simulation and theory calculations of interaction. In particular, 9 kinds of polyphenols compounds from Ligustrum robustum were firstly identified by UPLC-Q-TOF-MS. Moreover, the recovered polyphenols exhibited significant antioxidant, alpha-glucosidase inhibition, acetylcholinesterase inhibition and anticancer activity.
ESTHER : Qin_2023_Food.Chem_429_136828
PubMedSearch : Qin_2023_Food.Chem_429_136828
PubMedID: 37478601

Title : Study on Spontaneous Reactivation and Aging of Acetylcholinesterase Inhibited by Paraoxon and Malaoxon in Ten Species - Gao_2023_Int.J.Mol.Sci_24_
Author(s) : Gao M , Ni Z , Li G , Wu G , Huang B
Ref : Int J Mol Sci , 24 : , 2023
Abstract : Organophosphorus insecticides (OPs), acting as serine phosphorylating agents in acetylcholinesterase (AChE), are highly effective neurotoxic insecticides. In our previous research, we found that six herbivorous pests and four ladybirds howed significantly higher AChE LC50 values than seven parasitoids and a predator (Epistrophe balteate), and that there was a significant correlation with the corresponding bimolecular rate constant (Ki) value. The Ki value of pests was much smaller than that of natural enemies and had a higher LC50 value.Then, we speculated that the low sensitivity of the pest AChE to OPs may be associated with its higher recovery and lower aging ability. In this work, the I50 and I90 were calculated, to determine the sensibility of AChE in ten representative species, including Plutella xylostella, Prodenia litura, Musca domestica, and Cavia porcellus, to paraoxon and malaoxon. The enzyme activities were measured at various time points, and kinetic calculations were used to obtain their spontaneous reactivation (Ks) and aging (Ka) constants, which were comprehensively compared. We conclude that the Ka and Ks of the AChE inhibited by OPs showed primarily species-specific correlations, and little correlation with the sensitivity to OPs. The differences in the AChE sensitivity to paraoxon among the ten species were much greater than in the sensitivity to malaoxon. Compared to paraoxon, malaoxon was more selective for Cavia porcellus. Coleoptera insects showed a stronger dephosphorylation ability than other insect groups. The recovery ability of phospho-AChE was stronger in mammals than in insects, which could be related to the low sensitivity of the AChE site of action to OPs. The Ka of the AChE inhibited by malaoxon was larger than that inhibited by paraoxon with the corresponding biomaterials, indicating that the OP type had a substantial relationship with the Ka of the AChE. We further discovered that, when insects were inhibited by OP, the tendency of AChE to undergo aging was greater than that of dephosphorylation. Overall, the study provides valuable information on the action mechanism of various OPs on AChE in several species, which could be used to further research into AChE and the potential dangers that organophosphates pose to animals.
ESTHER : Gao_2023_Int.J.Mol.Sci_24_
PubMedSearch : Gao_2023_Int.J.Mol.Sci_24_
PubMedID: 37762515

Title : Habitual feeding patterns impact polystyrene microplastic abundance and potential toxicity in edible benthic mollusks - Wang_2023_Sci.Total.Environ_866_161341
Author(s) : Wang S , Zheng L , Shen M , Zhang L , Wu Y , Li G , Guo C , Hu C , Zhang M , Sui Y , Dong X , Lv L
Ref : Sci Total Environ , 866 :161341 , 2023
Abstract : That increasing microplastics (MPs, <5 mm) eventually end up in the sediment which may become a growing menace to diverse benthic lives is worthy of attention. In this experiment, three edible mollusks including one deposit-feeding gastropod (Bullacta exarate) and two filter-feeding bivalves (Cyclina sinensis and Mactra veneriformis) were exposed to polystyrene microplastic (PS-MP) for 7 days and depurated for 3 days. PS-MP numbers in the digestive system and non-digestive system, digestive enzymes, oxidative stress indexes, and a neurotoxicity index of three mollusks were determined at day 0, 3, 7, 8 and 10. After seven-day exposure, the PS-MP were found in all three mollusks' digestive and non-digestive systems. And PS-MP in M. veneriformis (9.57 +/- 2.19 items/individual) was significantly higher than those in C. sinensis (3.00 +/- 2.16 items/individual) and B. exarate (0.83 +/- 1.07 items/individual) at day 7. Three-day depuration could remove most of the PS-MP in the mollusks, and higher PS-MP clearance rates were found in filter-feeding C. sinensis (77.78 %) and M. veneriformis (82.59 %) compared to surface deposit-feeding B. exarate (50.00 %). The digestive enzymes of B. exarate significantly reacted to PS-MP exposure, while oxidative responses were found in C. sinensis. After three-day depuration, the changes of digestive enzymes and the oxidative states were fixed, but neurotoxicity induced by PS-MP was not recoverable. Besides, it is noteworthy that changes of digestive enzymes and acetylcholinesterase are related to feeding patterns.
ESTHER : Wang_2023_Sci.Total.Environ_866_161341
PubMedSearch : Wang_2023_Sci.Total.Environ_866_161341
PubMedID: 36603620

Title : Genome-wide association study of 17 serum biochemical indicators in a chicken F(2) resource population - Song_2023_BMC.Genomics_24_98
Author(s) : Song H , Li W , Li Y , Zhai B , Guo Y , Chen Y , Han R , Sun G , Jiang R , Li Z , Yan F , Li G , Liu X , Zhang Y , Tian Y , Kang X
Ref : BMC Genomics , 24 :98 , 2023
Abstract : BACKGROUND: Serum biochemical indicators are often regarded as direct reflections of animal metabolism and health. The molecular mechanisms underlying serum biochemical indicators metabolism of chicken (Gallus Gallus) have not been elucidated. Herein, we performed a genome-wide association study (GWAS) to identify the variation associated with serum biochemical indicators. The aim of this research was to broaden the understanding of the serum biochemical indicators in chickens. RESULTS: A GWAS of serum biochemical indicators was carried out on 734 samples from an F2 Gushix Anka chicken population. All chickens were genotyped by sequencing, 734 chickens and 321,314 variants were obtained after quality control. Based on these variants, a total of 236 single-nucleotide polymorphisms (SNPs) on 9 chicken chromosomes (GGAs) were identified to be significantly (-log(10)(P) > 5.72) associated with eight of seventeen serum biochemical indicators. Ten novel quantitative trait locis (QTLs) were identified for the 8 serum biochemical indicator traits of the F2 population. Literature mining revealed that the ALPL, BCHE, GGT2/GGT5 genes at loci GGA24, GGA9 and GGA15 might affect the alkaline phosphatase (AKP), cholinesterase (CHE) and gamma-glutamyl transpeptidase (GGT) traits, respectively. CONCLUSION: The findings of the present study may contribute to a better understanding of the molecular mechanisms of chicken serum biochemical indicator regulation and provide a theoretical basis for chicken breeding programs.
ESTHER : Song_2023_BMC.Genomics_24_98
PubMedSearch : Song_2023_BMC.Genomics_24_98
PubMedID: 36864386

Title : Frameshift coding sequence variants in the LPL gene: identification of two novel events and exploration of the genotype-phenotype relationship for variants reported to date - Zhang_2023_Lipids.Health.Dis_22_128
Author(s) : Zhang G , Hu Y , Yang Q , Pu N , Li G , Zhang J , Tong Z , Masson E , Cooper DN , Chen JM , Li W
Ref : Lipids Health Dis , 22 :128 , 2023
Abstract : BACKGROUND: Lipoprotein lipase (LPL) is the rate-limiting enzyme for triglyceride hydrolysis. Homozygous or compound heterozygous LPL variants cause autosomal recessive familial chylomicronemia syndrome (FCS), whereas simple heterozygous LPL variants are associated with hypertriglyceridemia (HTG) and HTG-related disorders. LPL frameshift coding sequence variants usually cause complete functional loss of the affected allele, thereby allowing exploration of the impact of different levels of LPL function in human disease. METHODS: All exons and flanking intronic regions of LPL were Sanger sequenced in patients with HTG-related acute pancreatitis (HTG-AP) or HTG-AP in pregnancy. Previously reported LPL frameshift coding sequence variants were collated from the Human Gene Mutation Database and through PubMed keyword searching. Original reports were manually evaluated for the following information: zygosity status of the variant, plasma LPL activity of the variant carrier, disease referred for genetic analysis, patient's age at genetic analysis, and patient's disease history. SpliceAI was employed to predict the potential impact of collated variants on splicing. RESULTS: Two novel rare variants were identified, and 53 known LPL frameshift coding sequence variants were collated. Of the 51 variants informative for zygosity, 30 were simple heterozygotes, 12 were homozygotes, and 9 were compound heterozygotes. Careful evaluation of the 55 variants with respect to their clinical and genetic data generated several interesting findings. First, we conclude that 6-7% residual LPL function could significantly delay the age of onset of FCS and reduce the prevalence of FCS-associated syndromes. Second, whereas a large majority of LPL frameshift coding sequence variants completely disrupt gene function through their "frameshift" nature, a small fraction of these variants may act wholly or partly as "in-frame" variants, leading to the generation of protein products with some residual LPL function. Third, we identified two candidate LPL frameshift coding sequence variants that may retain residual function based on genotype-phenotype correlation or SpliceAI-predicted data. CONCLUSIONS: This study reported two novel LPL variants and yielded new insights into the genotype-phenotype relationship as it pertains to LPL frameshift coding sequence variants.
ESTHER : Zhang_2023_Lipids.Health.Dis_22_128
PubMedSearch : Zhang_2023_Lipids.Health.Dis_22_128
PubMedID: 37568214
Gene_locus related to this paper: human-LPL

Title : Selective modulation of alkali metal ions on acetylcholinesterase - Mu_2023_Phys.Chem.Chem.Phys__
Author(s) : Mu X , Yuan S , Zhang D , Lai R , Liao C , Li G
Ref : Phys Chem Chem Phys , : , 2023
Abstract : Acetylcholinesterase (AChE) is an important hydrolase in cholinergic synapses and a candidate target in the treatment of Alzheimer's disease. The lithium treatment widely used in neurological disorders can alter the AChE activity, yet the underlying mechanism of how the ion species regulate the enzymatic activity remains unclear. In this work, we performed combined quantum mechanics/molecular mechanics (QM/MM) and molecular dynamics (MD) simulations and well-tempered metadynamics to understand the modulation of human AChE (hAChE) activity using three alkali metal ions (Li(+), Na(+), and K(+)) in different concentrations. Our simulations show that the binding affinity and catalytic activity are affected by different ion species through allosteric ion coordination geometries on the hAChE complex and distant electrostatic screening effect. A Li(+) cluster involving D330, E393, and D397 residues and three Li(+) ions was found to be highly conserved and can be critical to the enzyme activity. Binding energy calculations indicate that the electrostatic screening from allosterically bound cations can affect the key residues at the catalytic site and active-site gorge, including E199. Furthermore, an increase in ion concentration can lead to lower reactivity, especially for Li(+) ions, which exhibit more cation-hAChE contacts than Na(+) and K(+). The selective ion binding and their preferred modulation on hAChE are highly related to ion species. This work provides a molecular perspective on selective modulation by different ion species of the enzyme catalytic processes.
ESTHER : Mu_2023_Phys.Chem.Chem.Phys__
PubMedSearch : Mu_2023_Phys.Chem.Chem.Phys__
PubMedID: 37934509

Title : Lysosome-targeting phenalenones as efficient type I\/II photosensitizers for anticancer photodynamic therapy - Jiang_2023_Eur.J.Med.Chem_255_115418
Author(s) : Jiang Q , Li P , Qiu J , Li D , Li G , Shan G
Ref : Eur Journal of Medicinal Chemistry , 255 :115418 , 2023
Abstract : Development of safe and effective photosensitizers is important for enhancing the efficacy of photodynamic cancer therapy. Phenalenone is a type II photosensitizer with a high singlet oxygen quantum yield; however, its short UV absorption wavelength hinders its application in cancer imaging and in vivo photodynamic therapy. In this study, we report a new redshift phenalenone derivative, 6-amino-5-iodo-1H-phenalen-1-one (SDU Red [SR]), as a lysosome-targeting photosensitizer for triple-negative breast cancer therapy. SDU Red produced singlet oxygen (Type II reactive oxygen species [ROS]) and superoxide anion radicals (Type I ROS) upon light irradiation. It also exhibited good photostability and a remarkable phototherapeutic index (PI > 76) against triple-negative breast cancer MDA-MB-231 cancer cells. Additionally, we designed two amide derivatives, SRE-I and SRE-II, with decreased fluorescence and photosensitizing capabilities based on SDU Red as activatable photosensitizers for photodynamic cancer therapy. SRE-I and SRE-II could be further converted into the active photosensitizer SDU Red via carboxylesterase-catalyzed amide bond cleavage. Moreover, SDU Red and SRE-II induced DNA damage and cell apoptosis in the presence of light. Therefore, SRE-II can act as a promising theranostic agent for triple-negative breast cancer.
ESTHER : Jiang_2023_Eur.J.Med.Chem_255_115418
PubMedSearch : Jiang_2023_Eur.J.Med.Chem_255_115418
PubMedID: 37119664

Title : beta-Carbolines norharman and harman change neurobehavior causing neurological damage in Caenorhabditis elegans - Zhang_2023_Food.Funct_14_10031
Author(s) : Zhang L , Liu J , Xu B , Wu D , Wu Y , Li G
Ref : Food Funct , 14 :10031 , 2023
Abstract : beta-Carbolines norharman and harman, belonging to the class of heterocyclic aromatic amines (HAAs), are typical hazardous substances produced during the thermal processing of food. Compared to other HAAs, there have been limited reports on the toxicity of beta-carbolines. Nevertheless, the current studies are concerned with the neurotoxic effects of norharman and harman at high doses. It is still unknown whether the relatively low dose of beta-carbolines in foods induces neurotoxicity and the mechanism of the toxicity. In this study, C. elegans was exposed to a series of gradients of norharman and harman (0, 0.05, 5, and 10 mg L(-1)). The survival rate and indicators of ethology (locomotor behaviors, foraging behavior, and chemotaxis ability) were assessed. The antioxidant system and the contents of neurotransmitters, as well as the activity of acetylcholinesterase (AChE), were evaluated. Additionally, the RNA-seq screening of differentially expressed genes (DEGs) revealed the potential molecular mechanisms of norharman- and harman-induced toxic effects. Our results indicated that the risk of long-term exposure to norharman and harman at low doses (food-related doses) should be emphasized. Moreover, beta-carbolines might induce neurotoxicity by causing oxidative damage, regulating the content of neurotransmitters, and interfering with cytochrome P450 metabolism. This study would provide a toxicological basis for the neurotoxicity of beta-carbolines and lay the foundation for the risk assessment of endogenous pollutants in food.
ESTHER : Zhang_2023_Food.Funct_14_10031
PubMedSearch : Zhang_2023_Food.Funct_14_10031
PubMedID: 37927231

Title : Therapeutic potential of aromatic plant extracts in Alzheimer's disease: Comprehensive review of their underlying mechanisms - Ma_2023_CNS.Neurosci.Ther__
Author(s) : Ma Y , Li Y , Yin R , Guo P , Lei N , Li G , Xiong L , Xie Y
Ref : CNS Neurosci Ther , : , 2023
Abstract : AIMS: The aim of this review is to outline recent advancements in the application and mechanistic studies of aromatic plant extracts in Alzhermer`s disease (AD) to demonstrate their value in the management of this disease. BACKGROUND: AD is a neurodegenerative disease with a complex pathogenesis characterized by severe cognitive impairment. Currently, there are very few drugs available for the treatment of AD, and treatments are primarily focused on symptom relief. Aromatherapy is a traditional complementary alternative therapy that focuses on the prevention and treatment of the disease through the inhalation or transdermal administration of aromatic plant extracts. Over the past few years, studies on the use of aromatic plant extracts for the treatment of AD have been increasing and have demonstrated a definitive therapeutic effect. METHODS: We systematically summarized in vitro, in vivo, and clinical studies focusing on the potential use of aromatic plant extracts in the treatment of AD in PubMed, ScienceDirect, Google Scholar, and the Chinese National Knowledge Infrastructure from 2000 to 2022. RESULTS: Our literature survey indicates that aromatic plant extracts exert anti-AD effects by modulating pathological changes through anti-amyloid, anti-tau phosphorylation, anti-cholinesterase, anti-inflammation, and anti-oxidative stress mechanisms (Figure 1). CONCLUSION: This review provides a future strategy for the research of novel anti-AD drugs from aromatic plant extracts.
ESTHER : Ma_2023_CNS.Neurosci.Ther__
PubMedSearch : Ma_2023_CNS.Neurosci.Ther__
PubMedID: 37122144

Title : IL-33 Downregulates Hepatic Carboxylesterase 1 in Acute Liver Injury via Macrophage-derived Exosomal miR-27b-3p - Gao_2023_J.Clin.Transl.Hepatol_11_1130
Author(s) : Gao P , Li M , Lu J , Xiang D , Wang X , Xu Y , Zu Y , Guan X , Li G , Zhang C
Ref : J Clin Transl Hepatol , 11 :1130 , 2023
Abstract : BACKGROUND AND AIMS: We previously reported that carboxylesterase 1 (CES1) expression was suppressed following liver injury. The study aimed to explore the role of interleukin (IL)-33 in liver injury and examine the mechanism by which IL-33 regulates CES1. METHODS: IL-33 and CES1 levels were determined in the livers of patients and lipopolysaccharide (LPS)-, acetaminophen (APAP)-treated mice. We constructed IL-33 and ST2 knockout (KO) mice. ST2-enriched immune cells in livers were screened to identify the responsible cells. Macrophage-derived exosome (MDE) activity was tested by adding exosome inhibitors. Micro-RNAs (miRs) were extracted from control and IL-33-stimulated MDEs (IL-33-MDEs) and subjected miR sequencing (miR-Seq). Candidate miR was tested in vitro and in vivo and its binding of a target gene was assessed by luciferase reporter assays. Lentivirus-vector cellular transfection and transcript silencing were used to examine pathways mediating IL-33 suppression of miR-27b-3p. RESULTS: Patient liver IL-33 and CES1 expression levels were inversely correlated. CES1 downregulation in liver injury was rescued in both IL-33-deficient and ST2 KO mice. Macrophages were shown to be responsible for IL-33 effects. IL-33-MDEs reduced CES1 levels in hepatocytes. Exosomal miR-Seq and qRT-PCR demonstrated increased miR-27b-3p levels in IL-33-MDEs; miR-27b-3p was implicated in Nrf2 targeting. IL-33 inhibition of miR-27b-3p was found to be GATA3-dependent. CONCLUSIONS: IL-33-ST2-GATA3 pathway signaling increases miR-27b-3p content in MDEs, which upon being internalized by hepatocytes reduce CES1 expression by inhibiting Nrf2. The elucidation of this mechanism in this study contributes to a better understanding of CES1 dysregulation in liver injury.
ESTHER : Gao_2023_J.Clin.Transl.Hepatol_11_1130
PubMedSearch : Gao_2023_J.Clin.Transl.Hepatol_11_1130
PubMedID: 37577217

Title : Characterization of a novel thermostable alkaline lipase derived from a compost metagenomic library and its potential application in the detergent industry - Li_2022_Front.Microbiol_13_1088581
Author(s) : Li QQ , Zhu ZR , Liu QG , An YT , Wang YX , Zhang SB , Li G
Ref : Front Microbiol , 13 :1088581 , 2022
Abstract : Using composted soil samples, a metagenomic library consisting of 36,000 clones was constructed. Then, a novel lipase, Lip54q, which belongs to the VIII family of lipolytic enzymes, was identified from the metagenomic library by functional screening. To explore the enzymatic properties of Lip54q, lip54q was heterologous expressed in Escherichia coli with a high expression level of recombinant protein up to 720 mg/L. The recombinant enzyme showed the highest activity (28,160 U/mg) against a C10 substrate at pH 9.0 and 47 degreesC, and was stable at temperatures >=50 degreesC and pH 8.0-11.0. Of particular interest, the surfactants, Tween-20, Tween-80 and Tritonx-100, exhibited strong promoting effects on Lip54q activities regardless of whether low concentrations (0.1%) or high concentrations (10%) were used. Application studies of Lip54q using six commercial detergents indicated that the enzyme had strong tolerance and immersion resistance to all six detergents. The results of oil-stain removal experiments suggested that addition of the enzyme to various commercial detergents could significantly improve the abilities of these detergents to remove oil-stains. Furthermore, the results of a molecular docking analysis of Lip54q showed that both the C10 substrate and linoleic acid molecules could form hydrogen bond interactions with the catalytic amino acids, Ser-268, Glu-168, and Asp-192, in the catalytic center of the enzyme, and the hydrogen bond distances were shorter. The electrostatic attraction between the enzyme and the substrate formed by the hydrogen bond with a shorter distance is stronger, which is conducive to the formation of a more stable complex between the enzyme and the substrate, thus increasing the activity of the enzyme to such substrate. These results 1ay a good foundation for application of this enzyme in the detergent industry in the future.
ESTHER : Li_2022_Front.Microbiol_13_1088581
PubMedSearch : Li_2022_Front.Microbiol_13_1088581
PubMedID: 36620038

Title : Glimepiride Use is Associated with Reduced Cardiovascular Mortality in Patients with Type 2 Diabetes and Chronic Heart Failure: A Prospective Cohort Study - He_2022_Eur.J.Prev.Cardiol__
Author(s) : He W , Yuan G , Han Y , Yan Y , Li G , Zhao C , Shen J , Jiang X , Chen C , Ni L , Wang DW
Ref : Eur J Prev Cardiol , : , 2022
Abstract : BACKGROUND: Glimepiride has good cardiovascular safety. However, whether glimepiride benefits clinical cardiovascular outcomes is unclear. METHODS: A total of 21,451 inpatients with type 2 diabetes (T2D) and chronic heart failure (CHF) were analyzed, including 638 who received glimepiride treatment and 20,813 who did not. Propensity score matching yielded 509 pairs (glimepiride and non-glimepiride groups), and both groups were followed up. Kaplan-Meier and Cox regression analyses were used to compare all-cause mortality, cardiovascular mortality, hospitalizations and emergency visits for heart failure, and hospitalizations for acute myocardial infarction or stroke. RESULTS: During follow-up, the all-cause mortality (adjusted hazard ratio [HR], 0.47; 95% confidence interval [CI], 0.35-0.63; P < 0.001), cardiovascular mortality (adjusted HR, 0.34; 95% CI, 0.24-0.48; P < 0.001), and number of hospitalizations and emergency visits for heart failure (adjusted HR, 0.42; 95% CI, 0.36-0.50; P < 0.001) and hospitalizations for acute myocardial infarction or stroke (adjusted HR, 0.53; 95% CI, 0.38-0.73; P < 0.001) were significantly lower in the glimepiride group; the conclusion remained similar in all subgroups. Furthermore, high-dose glimepiride use (2-4mg/day) was associated with lower cardiovascular mortality than low-dose (1mg/day) (adjusted HR, 0.55; 95% CI, 0.31-0.99; P = 0.047). Glimepiride exhibited good molecular docking with soluble epoxide hydrolase (sEH) and increased the level epoxyeicosatrienoic acid (EET). CONCLUSIONS: Long-term continuous glimepiride use is associated with better survival, fewer hospitalizations and emergency visits for heart failure, and fewer hospitalizations for acute myocardial infarction or stroke in patients with T2D and CHF. High-dose glimepiride has greater cardiovascular protective advantages than low-dose glimepiride. The cardiovascular protective effect of glimepiride may be related to the EET level increase through sEH inhibition.
ESTHER : He_2022_Eur.J.Prev.Cardiol__
PubMedSearch : He_2022_Eur.J.Prev.Cardiol__
PubMedID: 36573717

Title : [Characterization of Humicola insolens cutinase-tachystatin A2 fusion protein and its application in treatment of recycled paper stickies] - Li_2022_Sheng.Wu.Gong.Cheng.Xue.Bao_38_207
Author(s) : Li G , Liu Z , Zhang Y , Wu J
Ref : Sheng Wu Gong Cheng Xue Bao , 38 :207 , 2022
Abstract : With the decrease of forest timber resources, the recycling of waste paper has received increasing attention. However, the stickies produced in the process of waste paper recycling may negatively affect the production of recycled paper. The biological decomposition of stickies, which has the advantages of high efficiency, high specificity and pollution-free, is achieved mainly through the enzymatic cleavage of the ester bond in the stickies components to prevent flocculation. Cutinase is a serine esterase that can degrade some components of the stickies. Previous research indicated that the anchor peptide tachystatin A2 (TA2) is able to bind polyurethane. In this study, the cutinase HiC derived from Humicola insolens was used to construct a fusion protein HiC-TA2 by megaprimer PCR of the whole plasmid (MEGAWHOP). The enzymatic properties and the degradation efficiency of the fusion protein on poly(ethyl acrylate) (PEA), a model substrate of stickies component, were determined. The results showed that the degradation efficiency, the size decrease of PEA particle, and the amount of ethanol produced by HiC-TA2 were 1.5 times, 6.8 times, and 1.4 times of that by HiC, respectively. These results demonstrated that TA2 improved the degradation efficiency of HiC on PEA. This study provides a useful reference for biological decomposition of stickies produced in the process of recycled paper production.
ESTHER : Li_2022_Sheng.Wu.Gong.Cheng.Xue.Bao_38_207
PubMedSearch : Li_2022_Sheng.Wu.Gong.Cheng.Xue.Bao_38_207
PubMedID: 35142131

Title : Adipose tissue-specific ablation of Ces1d causes metabolic dysregulation in mice - Li_2022_Life.Sci.Alliance_5_
Author(s) : Li G , Li X , Yang L , Wang S , Dai Y , Fekry B , Veillon L , Tan L , Berdeaux R , Eckel-Mahan K , Lorenzi PL , Zhao Z , Lehner R , Sun K
Ref : Life Sciences Alliance , 5 : , 2022
Abstract : Carboxylesterase 1d (Ces1d) is a crucial enzyme with a wide range of activities in multiple tissues. It has been reported to localize predominantly in ER. Here, we found that Ces1d levels are significantly increased in obese patients with type 2 diabetes. Intriguingly, a high level of Ces1d translocates onto lipid droplets where it digests the lipids to produce a unique set of fatty acids. We further revealed that adipose tissue-specific Ces1d knock-out (FKO) mice gained more body weight with increased fat mass during a high fat-diet challenge. The FKO mice exhibited impaired glucose and lipid metabolism and developed exacerbated liver steatosis. Mechanistically, deficiency of Ces1d induced abnormally large lipid droplet deposition in the adipocytes, causing ectopic accumulation of triglycerides in other peripheral tissues. Furthermore, loss of Ces1d diminished the circulating free fatty acids serving as signaling molecules to trigger the epigenetic regulations of energy metabolism via lipid-sensing transcriptional factors, such as HNF4alpha. The metabolic disorders induced an unhealthy microenvironment in the metabolically active tissues, ultimately leading to systemic insulin resistance.
ESTHER : Li_2022_Life.Sci.Alliance_5_
PubMedSearch : Li_2022_Life.Sci.Alliance_5_
PubMedID: 35459739
Gene_locus related to this paper: mouse-Ces1d

Title : Does butyrylcholinesterase mediate exercise-induced and meal-induced suppression in acylated ghrelin? - Li_2022_Endocr.J__
Author(s) : Li G , Tataka Y , Kamemoto K , Wen H , Yamanaka K , Nagayama C , Miyashita M
Ref : Endocrine Journal , : , 2022
Abstract : Ample evidence supports the notion that an acute bout of aerobic exercise and meal consumption reduces acylated ghrelin concentration. However, the mechanisms by which this exercise- and meal-induced suppression of acylated ghrelin occurs in humans is unknown. This study aimed to examine the concentration of butyrylcholinesterase (BChE), an enzyme responsible for hydrolysing ghrelin and other appetite-related hormones in response to a single bout of running and a standardised meal in young, healthy men. Thirty-three men (aged 23 +/- 2 years, mean +/- standard deviation) underwent two (exercise and meal conditions) 2-h laboratory-based experiments. In the exercise condition, all participants ran for 30 min at 70% of the maximum oxygen uptake (0930-1000) and rested until 1130. In the meal condition, participants reported to the laboratory at 0930 and rested until 1000. Subsequently, they consumed a standardised meal (1000-1015) and rested until 1130. Blood samples were collected at baseline (0930), 1000, 1030, 1100 and 1130. BChE concentration was not altered in both the exercise and meal conditions (p > 0.05). However, acylated ghrelin was suppressed after exercise (p < 0.05) and meal consumption (p < 0.05). There was no association between the change in BChE concentration and the change in acylated ghrelin before and after exercise (p = 0.571). Although des-acylated ghrelin concentration did not change during exercise (p > 0.05), it decreased after meal consumption (p < 0.05). These findings suggest that BChE may not be involved in the suppression of acylated ghrelin after exercise and meal consumption.
ESTHER : Li_2022_Endocr.J__
PubMedSearch : Li_2022_Endocr.J__
PubMedID: 36047188

Title : Enhanced biodegradation activity towards poly(ethyl acrylate) and poly(vinyl acetate) by anchor peptide assistant targeting - Liu_2022_J.Biotechnol__
Author(s) : Liu Z , Li G , Zhang F , Wu J
Ref : J Biotechnol , : , 2022
Abstract : The paper industry is one of the most important basic raw material pillar industries. With the decrease of forest wood resources, the recycling of wastepaper has drawn increasingly attention. However, the stickies generated in the process of wastepaper recycling will flocculate and deposite in the pulp, resulting in production accidents and inferior product quality. The biological enzymatic method, with the advantages of high efficiency, specificity, and pollution-free, can prevent the flocculation of the stickies by enzymatically hydrolyzing the ester bond of the stickies components. Previous studies have demonstrated that cutinase (EC 3.1.1.74) had the ability to degrade polyester components of stickies. Meanwhile, relevant studies have shown that anchor peptides possessed the ability to bind polyester. Herein, the cutinase from Humicola insolens (HiC) was fused with Escherichia coli anchor peptide OMP25, the enzymatic properties of the fusion protein HiC-OMP25 and its degradation efficiency of the stickies model substrate, poly(ethyl acrylate) (PEA) and poly(vinyl acetate) (PVAc), as well as stickies sediment were determined. All of the results demonstrated that OMP25 efficiently enhanced the degradation ability of HiC.
ESTHER : Liu_2022_J.Biotechnol__
PubMedSearch : Liu_2022_J.Biotechnol__
PubMedID: 35292345

Title : Improved pea reference genome and pan-genome highlight genomic features and evolutionary characteristics - Yang_2022_Nat.Genet_54_1553
Author(s) : Yang T , Liu R , Luo Y , Hu S , Wang D , Wang C , Pandey MK , Ge S , Xu Q , Li N , Li G , Huang Y , Saxena RK , Ji Y , Li M , Yan X , He Y , Liu Y , Wang X , Xiang C , Varshney RK , Ding H , Gao S , Zong X
Ref : Nat Genet , 54 :1553 , 2022
Abstract : Complete and accurate reference genomes and annotations provide fundamental resources for functional genomics and crop breeding. Here we report a de novo assembly and annotation of a pea cultivar ZW6 with contig N50 of 8.98 Mb, which features a 243-fold increase in contig length and evident improvements in the continuity and quality of sequence in complex repeat regions compared with the existing one. Genome diversity of 118 cultivated and wild pea demonstrated that Pisum abyssinicum is a separate species different from P. fulvum and P. sativum within Pisum. Quantitative trait locus analyses uncovered two known Mendel's genes related to stem length (Le/le) and seed shape (R/r) as well as some candidate genes for pod form studied by Mendel. A pan-genome of 116 pea accessions was constructed, and pan-genes preferred in P. abyssinicum and P. fulvum showed distinct functional enrichment, indicating the potential value of them as pea breeding resources in the future.
ESTHER : Yang_2022_Nat.Genet_54_1553
PubMedSearch : Yang_2022_Nat.Genet_54_1553
PubMedID: 36138232
Gene_locus related to this paper: pea-a0a9d4zt76

Title : Butenolide derivatives from Aspergillus terreus selectively inhibit butyrylcholinesterase - Cui_2022_Front.Chem_10_1063284
Author(s) : Cui X , Deng S , Li G , Zhang Y , Wang L , Wu C , Deng Y
Ref : Front Chem , 10 :1063284 , 2022
Abstract : Two undescribed butenolide derivatives, asperteretal J (1) and K (2), together with 13 known ones (3-15) were isolated from an endophytic fungus Aspergillus terreus SGP-1, the fermentation product of which exhibited selective inhibitory activity toward butyrylcholinesterase. The structures of the new compounds were elucidated based on HRMS and NMR data, and the absolute configurations were determined by specific optical rotation comparison. All compounds were evaluated for cholinesterase inhibitory effects with galantamine as a positive control. Compounds 4-8 selectively inhibited butyrylcholinesterase with IC(50) values of 18.4-45.8smicroM in a competitive manner, with Ki values of 12.3-38.2smicroM. The structure-activity relationship was discussed. Molecular docking and dynamic simulation of the inhibitor-enzyme complex were performed to better understand the interactions.
ESTHER : Cui_2022_Front.Chem_10_1063284
PubMedSearch : Cui_2022_Front.Chem_10_1063284
PubMedID: 36618870

Title : Identification and Characterization of Two Novel Compounds: Heterozygous Variants of Lipoprotein Lipase in Two Pedigrees With Type I Hyperlipoproteinemia - Wang_2022_Front.Endocrinol.(Lausanne)_13_874608
Author(s) : Wang S , Cheng Y , Shi Y , Zhao W , Gao L , Fang L , Jin X , Han X , Sun Q , Li G , Zhao J , Xu C
Ref : Front Endocrinol (Lausanne) , 13 :874608 , 2022
Abstract : BACKGROUND: Type I hyperlipoproteinemia, characterized by severe hypertriglyceridemia, is caused mainly by loss-of-function mutation of the lipoprotein lipase (LPL) gene. To date, more than 200 mutations in the LPL gene have been reported, while only a limited number of mutations have been evaluated for pathogenesis. OBJECTIVE: This study aims to explore the molecular mechanisms underlying lipoprotein lipase deficiency in two pedigrees with type 1 hyperlipoproteinemia. METHODS: We conducted a systematic clinical and genetic analysis of two pedigrees with type 1 hyperlipoproteinemia. Postheparin plasma of all the members was used for the LPL activity analysis. In vitro studies were performed in HEK-293T cells that were transiently transfected with wild-type or variant LPL plasmids. Furthermore, the production and activity of LPL were analyzed in cell lysates or culture medium. RESULTS: Proband 1 developed acute pancreatitis in youth, and her serum triglycerides (TGs) continued to be at an ultrahigh level, despite the application of various lipid-lowering drugs. Proband 2 was diagnosed with type 1 hyperlipoproteinemia at 9 months of age, and his serum TG levels were mildly elevated with treatment. Two novel compound heterozygous variants of LPL (c.3G>C, p. M1? and c.835_836delCT, p. L279Vfs*3, c.188C>T, p. Ser63Phe and c.662T>C, p. Ile221Thr) were identified in the two probands. The postheparin LPL activity of probands 1 and 2 showed decreases of 72.22 +/- 9.46% (p<0.01) and 54.60 +/- 9.03% (p<0.01), respectively, compared with the control. In vitro studies showed a substantial reduction in the expression or enzyme activity of LPL in the LPL variants. CONCLUSIONS: Two novel compound heterozygous variants of LPL induced defects in the expression and function of LPL and caused type I hyperlipoproteinemia. The functional characterization of these variants was in keeping with the postulated LPL mutant activity.
ESTHER : Wang_2022_Front.Endocrinol.(Lausanne)_13_874608
PubMedSearch : Wang_2022_Front.Endocrinol.(Lausanne)_13_874608
PubMedID: 35923617
Gene_locus related to this paper: human-LPL

Title : Interfering with lipid metabolism through targeting CES1 sensitizes hepatocellular carcinoma for chemotherapy - Li_2022_JCI.Insight__
Author(s) : Li G , Li X , Mahmud I , Ysaguirre J , Fekry B , Wang S , Wei B , Eckel-Mahan KL , Lorenzi PL , Lehner R , Sun K
Ref : JCI Insight , : , 2022
Abstract : Hepatocellular carcinoma (HCC) is the most common lethal form of liver cancer. Apart from surgical removal and transplantation, other treatments have not yet been well established for patients with HCC. Herein, we found that carboxylesterase 1 (CES1) was expressed at various levels in HCC. We further revealed that blockage of CES1 by pharmacological and genetical approaches leads to altered lipid profiles that are directly linked to impaired mitochondrial function. Mechanistically, LC-MS/MS and lipidomic analyses revealed that lipid signaling molecules, including polyunsaturated fatty acids (PUFAs), which activate PPARalpha/gamma, were dramatically reduced upon CES1 inhibition. As a result, SCD, a PPARalpha/gamma target gene involved in tumor progression and chemoresistance, was significantly downregulated. Consistently, clinical analysis demonstrated a strong correlation between the protein levels of CES1 and SCD in HCC. Interference with lipid signaling by targeting the "CES1-PPARalpha/gamma-SCD" axis sensitized HCC cells to cisplatin treatment. As a demonstration, the growth of HCC xenograft tumors in NU/J mice was potently limited by co-administration of cisplatin and CES1 inhibition. Our results suggest that CES1 is a promising therapeutic target for HCC treatment.
ESTHER : Li_2022_JCI.Insight__
PubMedSearch : Li_2022_JCI.Insight__
PubMedID: 36472914

Title : Predictive value of serum cholinesterase in the mortality of acute pancreatitis: A retrospective cohort study - Wei_2022_Eur.J.Clin.Invest__e13741
Author(s) : Wei M , Xie X , Yu X , Lu Y , Ke L , Ye B , Zhou J , Li G , Li B , Tong Z , Lu G , Li W , Li J
Ref : European Journal of Clinical Investigation , :e13741 , 2022
Abstract : BACKGROUND: Severe acute pancreatitis has a high mortality of 20-40%, but there is lack of optimal prognostic biomarker for the severity of acute pancreatitis (AP) or mortality. This study is designed to investigate the relationship between serum cholinesterase (ChE) level and poor outcomes of AP. METHODS: A total of 1904 AP patients were screened in the study, and we finally got 692 patients eligible for analysis. Patients were divided into 2 groups based on serum ChE. The primary outcome was mortality, and multivariable logistic regression analysis for mortality was completed. Additionally, we used receiver operator characteristic (ROC) curve analysis to clarify the predictive value of serum ChE for mortality and organ failure. RESULTS: 378 patients and 314 patients were included in ChE low and normal group, respectively. Patients in ChE low group were older (46.68+/-12.70 vs 43.56+/-12.13 years old, p=0.001) and had a lower percentage of male (62.4% vs 71.0%, p=0.017) when compared with the ChE normal group. Mortality was significantly different in two groups (10.3% vs 0.0%, p<0.001). Moreover, organ failure also differed significantly in two groups (46.6% vs 8.6%, p<0.001). Decreased ChE level was independently associated with mortality in acute pancreatitis (odds ratio: 0.440; 95% confidence interval, 0.231, 0.838, p=0.013). The area under the curve of serum ChE was 0.875 and 0.803 for mortality and organ failure, respectively. CONCLUSIONS: Lower level of serum ChE was independently associated with the severity and mortality of AP.
ESTHER : Wei_2022_Eur.J.Clin.Invest__e13741
PubMedSearch : Wei_2022_Eur.J.Clin.Invest__e13741
PubMedID: 34981831

Title : GPIHBP1 autoantibody is an independent risk factor for the recurrence of hypertriglyceridemia-induced acute pancreatitis - Zhang_2022_J.Clin.Lipidol__
Author(s) : Zhang G , Yang Q , Mao W , Hu Y , Pu N , Deng H , Yu X , Zhang J , Zhou J , Ye B , Li G , Li B , Ke L , Tong Z , Murakami M , Kimura T , Nakajima K , Cao W , Liu Y , Li W
Ref : J Clin Lipidol , : , 2022
Abstract : BACKGROUND: GPIHBP1, a glycolipid-anchored protein of capillary endothelial cells, is a crucial partner for lipoprotein lipase (LPL) in plasma triglyceride metabolism. GPIHBP1 autoantibodies block LPL binding to GPIHBP1 and lead to severe hypertriglyceridemia (HTG) and HTG-induced acute pancreatitis (HTG-AP). We sought to define the incidence of GPIHBP1 autoantibodies in patients with HTG-AP. OBJECTIVE: We determined the incidence of GPIHBP1 autoantibody in HTG-AP patients, and compared the clinical features and long-term outcomes between GPIHBP1 autoantibody-positive and negative groups. METHODS: An enzyme-linked immunosorbent assay was used to screen for GPIHBP1 autoantibody in 116 HTG-AP patients hospitalized from Jan 1, 2015 to Aug 31, 2019. All patients were followed up for 24 months. The primary outcome was the recurrence rate of HTG-AP during the two-year follow-up period. The incidence of recurrent episodes was analyzed by the Kaplan-Meier method and multivariable Cox regression was used to identify risk factors. RESULTS: GPIHBP1 autoantibodies were present in 17 of 116 study patients (14.66%). The 2-year recurrence rate of HTG-AP was much higher in the GPIHBP1 autoantibody-positive group (35%, 6 in 17) than in the negative group (4%, 4 in 99). The multivariable Cox regression analysis showed that GPIHBP1 autoantibody was an independent risk factor for HTG-AP recurrence in two years. CONCLUSIONS: The presence of GPIHBP1 autoantibody is common in patients with HTG-AP, and is an independent risk factor for two-year recurrence of HTG-AP.
ESTHER : Zhang_2022_J.Clin.Lipidol__
PubMedSearch : Zhang_2022_J.Clin.Lipidol__
PubMedID: 36064883

Title : OsMas1, a novel maspardin protein gene, confers the tolerance to salt and drought stresses by regulating ABA signaling in rice - Wang_2022_J.Integr.Agric__
Author(s) : Wang FB , Wan CZ , Niu HF , Qi MY , Li G , Zhang F , Hu LB , Ye YX , Wang ZX , Pei BL , Chen XH , Yuan CY
Ref : J.Integr.Agric , : , 2022
Abstract : Drought and salt stresses, as major environmental abiotic stresses in agriculture worldwide, affect plant growth and crop productivity and quality. The development of crops with higher drought and salt tolerance is therefore highly desirable. Here, we reported the isolation and biological function and molecular characterization of a novel maspardin gene OsMas1 from rice. The OsMas1 protein was localized to the cytoplasm. The expression levels of OsMas1 were up-regulated under mannitol, PEG6000, NaCl and ABA treatments in rice. The OsMas1 gene was introduced into rice cultivar Zhonghua 11 (wild-type, WT) and OsMas1-overexpression (OsMas1-OE) plants exhibited significantly enhanced salt and drought tolerance, while OsMas1-interference (OsMas1-RNAi) plants exhibited decreased tolerance to salt and drought stresses, compared with WT. OsMas1-OE plants exhibited enhanced hypersensitive, while OsMas1-RNAi plants showed less sensitive to exogenous ABA treatment at both germination and post-germination stages. ABA, proline and K+ content and superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and photosynthesis activities were significantly increased, while malonaldehyde (MDA), hydrogen peroxide (H2O2), superoxide anion radical (O2-) and Na+ content were significantly decreased in OsMas1-OE plants compared with OsMas1-RNAi and WT plants. Overexpression of OsMas1 up-regulated the genes involved in ABA signaling, proline biosynthesis, ROS-scavenging system, photosynthesis and ion transport under salt and drought stresses. Collectively, our results indicate that OsMas1 gene functions in improving salt and drought tolerance in rice, which may serve as a candidate gene for enhancing the resistance to abiotic stresses in crops.
ESTHER : Wang_2022_J.Integr.Agric__
PubMedSearch : Wang_2022_J.Integr.Agric__
PubMedID:
Gene_locus related to this paper: orysa-q33aq0

Title : Cholinesterase homozygous genotype as susceptible biomarker of hypertriglyceridemia for pesticide-exposed agricultural workers - Zhou_2021_Biomarkers__1
Author(s) : Zhou X , Zhang M , Wang Y , Xia H , Zhu L , Li G , Rong L , Dong H , Chen R , Tang S , Yu M
Ref : Biomarkers , :1 , 2021
Abstract : PURPOSE: Dyslipidemia is an emerging metabolic disorder among pesticide-exposed agricultural workers, and this study was aimed to explore biomarkers of hypertriglyceridemia susceptibility. METHODS: This cross-sectional study recruited 72 pesticide-exposed subjects and 78 non-exposed controls. Lipid profile, cholinesterase activity, and thyroid hormones were analyzed with routine assays. Six loci, including rs11206244 and rs2235544 for deiodinase 1, rs12885300 and rs225014 for deiodinase 2, rs1803274 for butyrylcholinesterase, and rs3757869 for acetylcholinesterase were genotyped using an improved multiplex ligation detection reaction technique. RESULTS: Pesticide-exposed subjects showed higher levels of triglyceride than controls (p = 0.009), although there were comparable cholinesterase activity and genotype frequencies of all six loci between pesticide-exposed subjects and controls. Pesticide-exposed subjects with homozygous genotype of cholinesterasehad increased triglyceride levels than controls (p < 0.05). The percentage of hypertriglyceridemia was 28.6% and 8.8% for pesticide-exposed subjects and controls with homozygous butyrylcholinesterase genotype (p = 0.007) and 20.8% and 14.3% with homozygous acetylcholinesterase genotype (p = 0.792), respectively. Multivariate logistic regression analyses found that odds ratio of hypertriglyceridemia is 21.92 and 4.56 for pesticide-exposed subjects with homozygous genotype of butyrylcholinesterase (p = 0.001) and acetylcholinesterase (p = 0.036), respectively. CONCLUSIONS: Cholinesterase homozygous genotype might be a potential susceptible biomarker in screening pesticide-exposed agricultural workers vulnerable to hypertriglyceridemia.
ESTHER : Zhou_2021_Biomarkers__1
PubMedSearch : Zhou_2021_Biomarkers__1
PubMedID: 33617373

Title : A novel transcription factor UvCGBP1 regulates development and virulence of rice false smut fungus Ustilaginoidea virens - Chen_2021_Virulence_12_1563
Author(s) : Chen X , Li P , Liu H , Huang J , Luo C , Li G , Hsiang T , Collinge DB , Zheng L
Ref : Virulence , 12 :1563 , 2021
Abstract : Ustilaginoidea virens, causing rice false smut (RFS) is an economically important ascomycetous fungal pathogen distributed in rice-growing regions worldwide. Here, we identified a novel transcription factor UvCGBP1 (Cutinase G-box binding protein) from this fungus, which is unique to ascomycetes. Deletion of UvCGBP1 affected development and virulence of U. virens. A total of 865 downstream target genes of UvCGBP1 was identified using ChIP-seq and the most significant KEGG enriched functional pathway was the MAPK signaling pathway. Approximately 36% of target genes contain the AGGGG (G-box) motif in their promoter. Among the targets, deletion of UvCGBP1 affected transcriptional and translational levels of UvPmk1 and UvSlt2, both of which were important in virulence. ChIP-qPCR, yeast one-hybrid and EMSA confirmed that UvCGBP1 can bind the promoter of UvPmk1 or UvSlt2. Overexpression of UvPmk1 in the deltaUvCGBP1-33 mutant restored partially its virulence and hyphae growth, indicating that UvCGBP1 could function via the MAPK pathway to regulate fungal virulence. Taken together, this study uncovered a novel regulatory mechanism of fungal virulence linking the MAPK pathway mediated by a G-box binding transcription factor, UvCGBP1.
ESTHER : Chen_2021_Virulence_12_1563
PubMedSearch : Chen_2021_Virulence_12_1563
PubMedID: 34348597

Title : An uncharacterized protein from the metagenome with no obvious homology to known lipases shows excellent alkaline lipase properties and potential applications in the detergent industry - Xiao_2021_Biotechnol.Lett__
Author(s) : Xiao Y , Liu YD , Yuan G , Mao RQ , Li G
Ref : Biotechnol Lett , : , 2021
Abstract : A novel lipase, Lip486, which has no obvious homology with known lipases, was discovered using functional metagenomics technology. Phylogenetic tree analysis suggested that the enzyme belongs to a new subfamily called lipolytic enzyme family II. To explore the enzymatic properties, lip486 was expressed heterologously and efficiently in Escherichia coli. The recombinant enzyme displayed the highest activity on the substrate p-nitrophenyl caprate with a carbon chain length of 10, and its optimum temperature and pH were 53 degreesC and 8.0, respectively. The recombinant Lip486 showed good activity and stability in strong alkaline and medium-low-temperature environments. The results of compatibility and soaking tests showed that the enzyme had good compatibility with 4 kinds of commercial detergents, and an appropriate soaking time could further improve the enzyme activity. Oil stain removal test results for a cotton cloth indicated that the washing performance of commercial laundry detergent supplemented with Lip486 was further improved. In addition, as one of the smallest lipases found to date, Lip486 also has the advantages of high yield, good stability and easy molecular modification. These characteristics reflect the good application prospects for Lip486 in the detergent and other industries in the future.
ESTHER : Xiao_2021_Biotechnol.Lett__
PubMedSearch : Xiao_2021_Biotechnol.Lett__
PubMedID: 34698972

Title : Characterization of a novel sn1,3 lipase from Ricinus communis L. suitable for production of oleic acid-palmitic acid-glycerol oleate - Li_2021_Sci.Rep_11_6913
Author(s) : Li Y , Li G , Sun H , Chen Y
Ref : Sci Rep , 11 :6913 , 2021
Abstract : The hydrolysis properties of lipase in castor was evaluated using two different substrate forms (tripalmitic glycerides and trioleic glycerides) to catalyze the reaction under different operational conditions. RcLipase was obtained from castor seeds and results show that RcLipase is a conservative serine lipase with a conserved catalytic center (SDH) and a conserved pentapeptide (GXSXG). This enzyme exhibited the greatest activity and tolerance to chloroform and toluene when it was expressed in Pichia pastoris GS115 at 40 degC and pH 8.0. Zn and Cu ions exerted obvious inhibitory effects on the enzyme, and displayed good hydrolytic activity for long-chain natural and synthetic lipids. HPLC analysis showed that this enzyme has 1,3 regioselectivity when glycerol tripalmitate and oleic acid are used as substrates. The fatty acid composition in the reaction product was 21.3% oleic acid and 79.1% sn-2 palmitic acid.
ESTHER : Li_2021_Sci.Rep_11_6913
PubMedSearch : Li_2021_Sci.Rep_11_6913
PubMedID: 33767251
Gene_locus related to this paper: ricco-a0a0d3l472

Title : Celastrol Attenuates Learning and Memory Deficits in an Alzheimer's Disease Rat Model - Xiao_2021_Biomed.Res.Int_2021_5574207
Author(s) : Xiao Y , Wang X , Wang S , Li J , Xu X , Wang M , Li G , Shen W
Ref : Biomed Res Int , 2021 :5574207 , 2021
Abstract : Alzheimer's disease (AD) is a chronic progressive neurodegenerative disorder that is associated with learning, memory, and cognitive deficits. Neuroinflammation and synapse loss are involved in the pathology of AD. Diverse measures have been applied to treat AD, but currently, there is no effective treatment. Celastrol (CEL) is a pentacyclic triterpene isolated from Tripterygium wilfordii Hook F that has been shown to enhance cell viability and inhibit amyloid-beta production induced by lipopolysaccharides in vitro. In the present study, the protective effect of CEL on Abeta (25-35)-induced rat model of AD was assessed. Our results showed that CEL administration at a dose of 2 mg/kg/day improved spatial memory in the Morris water maze. Further biochemical analysis showed that CEL treatment of intrahippocampal Abeta (25-35)-microinjected rats attenuated hippocampal NF-kappaB activity; inhibited proinflammatory markers, namely, IL-1beta, IL-6, and TNF-alpha; and upregulated anti-inflammatory factors, such as IL-4 and IL-10. Furthermore, CEL upregulated hippocampal neurexin-1beta, neuroligin-1, CA1, and PSD95 expression levels, which may improve synaptic function. Simultaneously, CEL also increased glucose metabolism in Abeta (25-35)-microinjected rats. In conclusion, CEL could exert protective effects against learning and memory decline induced by intrahippocampal Abeta (25-35) through anti-inflammation, promote synaptic development, and maintain hippocampal energy metabolism.
ESTHER : Xiao_2021_Biomed.Res.Int_2021_5574207
PubMedSearch : Xiao_2021_Biomed.Res.Int_2021_5574207
PubMedID: 34350293

Title : Identification of Candidate Carboxylesterases Associated With Odorant Degradation in Holotrichia parallela Antennae Based on Transcriptome Analysis - Yi_2021_Front.Physiol_12_674023
Author(s) : Yi J , Wang S , Wang Z , Wang X , Li G , Zhang X , Pan Y , Zhao S , Zhang J , Zhou JJ , Wang J , Xi J
Ref : Front Physiol , 12 :674023 , 2021
Abstract : Insects rely on their olfactory systems in antennae to recognize sex pheromones and plant volatiles in surrounding environments. Some carboxylesterases (CXEs) are odorant-degrading enzymes (ODEs), degrading odorant signals to protect the olfactory neurons against continuous excitation. However, there is no report about CXEs in Holotrichia parallela, one of the most major agricultural underground pests in China. In the present study, 20 candidate CXEs were identified based on transcriptome analysis of female and male antennae. Sequence alignments and phylogenetic analysis were performed to investigate the characterization of these candidate CXEs. The expression profiles of CXEs were compared by RT-qPCR analysis between olfactory and non-olfactory tissues of both genders. HparCXE4, 11, 16, 17, 18, 19, and 20 were antenna-biased expressed genes, suggesting their possible roles as ODEs. HparCXE6, 10, 11, 13, and 16 showed significantly higher expression profiles in male antennae, whereas HparCXE18 was expressed more in female antennae. This study highlighted candidate CXE genes linked to odorant degradation in antennae, and provided a useful resource for further work on the H. parallela olfactory mechanism and selection of target genes for integrative control of H. parallela.
ESTHER : Yi_2021_Front.Physiol_12_674023
PubMedSearch : Yi_2021_Front.Physiol_12_674023
PubMedID: 34566671

Title : Physiological and transcriptomic changes of zebrafish (Danio rerio) embryos-larvae in response to 2-MIB exposure - Zhou_2021_J.Hazard.Mater_416_126142
Author(s) : Zhou W , Li X , Wang Y , Wang J , Zhang J , Wei H , Peng C , Wang Z , Li G , Li D
Ref : J Hazard Mater , 416 :126142 , 2021
Abstract : 2-Methylisoborneol (2-MIB), a natural odorous substance, is widely distributed in water environment, but there is a paucity of information concerning its systemic toxicity. Herein, we investigated the effects of 2-MIB exposure on developmental parameters, locomotive behavior, oxidative stress, apoptosis and transcriptome of zebrafish. Zebrafish embryos exposed to different concentrations (0, 0.5, 5 and 42.8 microg/L) of 2-MIB showed no changes in mortality, hatchability, and malformation rate, but the body length of zebrafish larvae was significantly increased in a dose-dependent manner, and accompanied by the changes of growth hormone/insulin-like growth factor (GH/IGF) axis and the hypothalamic-pituitary-thyroid (HPT) axis genes. Moreover, the swimming activity of zebrafish larvae increased, which may be due to the increase of acetylcholinesterase (AChE) activity. Meanwhile, 2-MIB caused oxidative stress and apoptosis in zebrafish larvae by altering the NF-E2-related factor 2 (Nrf2) and mitochondrial signaling pathways, respectively. Transcriptome sequencing assay showed that the phototransduction signaling pathway was significantly enriched, and most of the genes in this pathway exhibited enhanced expression after exposure to 2-MIB. These findings provide an important reference for risk assessment and early warning to 2-MIB exposure.
ESTHER : Zhou_2021_J.Hazard.Mater_416_126142
PubMedSearch : Zhou_2021_J.Hazard.Mater_416_126142
PubMedID: 34492931

Title : The cholinergic system, intelligence, and dental fluorosis in school-aged children with low-to-moderate fluoride exposure - Wang_2021_Ecotoxicol.Environ.Saf_228_112959
Author(s) : Wang S , Zhao Q , Li G , Wang M , Liu H , Yu X , Chen J , Li P , Dong L , Zhou G , Cui Y , Liu L , Wang A
Ref : Ecotoxicology & Environmental Safety , 228 :112959 , 2021
Abstract : Disruption of cholinergic neurotransmission can affect cognition, but little is known about whether low-to-moderate fluoride exposure affects cholinergic system and its effect on the prevalence of dental fluorosis (DF) and intelligence quotient (IQ). A cross-sectional study was conducted to explore the associations of moderate fluoride exposure and cholinergic system in relation to children's DF and IQ. We recruited 709 resident children in Tianjin, China. Ion selective electrode method was used to detect fluoride concentrations in water and urine. Cholinergic system was assessed by the detection of choline acetyltransferase (ChAT), acetylcholinesterase (AChE) and acetylcholine (ACh) levels in serum. Compared with children in the first quartile, those in fourth quartile the risk of either developing DF or IQ < 120 increased by 19% and 20% for water and urinary fluoride. The risk of having both increased by 58% and 62% in third and fourth quartile for water fluoride, 52% and 65% for urinary fluoride. Water fluoride concentrations were positively associated with AChE and negatively associated with ChAT and ACh, trends were same for urinary fluoride except for ACh. The risk of either developing DF or having non-high intelligence rose by 22% (95%CI: 1.07%, 1.38%) for the fourth quartile than those in the first quartile of AChE, for having the both, the risk was 1.27 (95%CI: 1.07, 1.50), 1.37 (95%CI: 1.17, 1.62) and 1.44 (95%CI: 1.23, 1.68) in second, third and fourth quartiles. The mediation proportion by AChE between water fluoride and either developing DF or IQ < 120 was 15.7%. For both to exist, the proportion was 6.7% and 7.2% for water and urinary fluoride. Our findings suggest low-to-moderate fluoride exposure was associated with dysfunction of cholinergic system for children. AChE may partly mediate the prevalence of DF and lower probability of having superior and above intelligence.
ESTHER : Wang_2021_Ecotoxicol.Environ.Saf_228_112959
PubMedSearch : Wang_2021_Ecotoxicol.Environ.Saf_228_112959
PubMedID: 34808511

Title : Anti-tubercular derivatives of rhein require activation by the monoglyceride lipase Rv0183 - Abrahams_2020_Cell.Surf_6_100040
Author(s) : Abrahams KA , Hu W , Li G , Lu Y , Richardson EJ , Loman NJ , Huang H , Besra GS
Ref : Cell Surf , 6 :100040 , 2020
Abstract : The emergence and perseverance of drug resistant strains of Mycobacterium tuberculosis (Mtb) ensures that drug discovery efforts remain at the forefront of tuberculosis research. There are numerous different approaches that can be employed to lead to the discovery of anti-tubercular agents. In this work, we endeavored to optimize the anthraquinone chemical scaffold of a known drug, rhein, converting it from a compound with negligible activity against Mtb, to a series of compounds with potent activity. Two compounds exhibited low toxicity and good liver microsome stability and were further progressed in attempts to identify the biological target. Whole genome sequencing of resistant isolates revealed inactivating mutations in a monoglyceride lipase. Over-expression trials and an enzyme assay confirmed that the designed compounds are prodrugs, activated by the monoglyceride lipase. We propose that rhein is the active moiety of the novel compounds, which requires chemical modifications to enable access to the cell through the extensive cell wall structure. This work demonstrates that re-engineering of existing antimicrobial agents is a valid method in the development of new anti-tubercular compounds.
ESTHER : Abrahams_2020_Cell.Surf_6_100040
PubMedSearch : Abrahams_2020_Cell.Surf_6_100040
PubMedID: 32743152
Gene_locus related to this paper: myctu-rv0183

Title : Immobilization of thermophilic lipase in inorganic hybrid nanoflower through biomimetic mineralization - Liu_2020_Colloids.Surf.B.Biointerfaces_197_111450
Author(s) : Liu Y , Shao X , Kong D , Li G , Li Q
Ref : Colloids Surf B Biointerfaces , 197 :111450 , 2020
Abstract : Thermophilic lipase QLM from Alcaligenes sp. was successfully immobilized in Cu(3)(PO(4))(2)-based inorganic hybrid nanoflower through biomimetic mineralization. The morphology, structure and element composition of immobilized enzyme were systemically characterized to elucidate the successful loading of enzyme molecules. The optimal temperature (65 degreeC) and pH (8.0) of immobilized enzyme were then determined by monitoring the hydrolysis of p-nitrophenyl caprylate. Moreover, compared with free enzyme, immobilized enzyme in inorganic hybrid nanoflower exhibited enhanced stability against thermal, pH and metal ions, attributing to the protective effect of nanoflower shell. Additionally, the immobilized enzyme possessed excellent reusability and long-term storage stability, with slightly decreased activity after being repeatedly used for 8 cycles or stored in water at room temperature for 4 weeks. Overall, the immobilization in inorganic hybrid nanoflower provided a facile and effective approach for the preparation of immobilized enzymes with favorable activity, stability and reusability, and thus the strategy showed great potential in developing ideal catalysts for future biocatalytic applications.
ESTHER : Liu_2020_Colloids.Surf.B.Biointerfaces_197_111450
PubMedSearch : Liu_2020_Colloids.Surf.B.Biointerfaces_197_111450
PubMedID: 33181387

Title : Identification of Oliver-McFarlane syndrome caused by novel compound heterozygous variants of PNPLA6 - Liu_2020_Gene__145027
Author(s) : Liu F , Ji Y , Li G , Xu C , Sun Y
Ref : Gene , :145027 , 2020
Abstract : OBJECTIVES: Oliver-McFarlane syndrome (OMCS) is an autosomal recessive inherited disease resulting from PNPLA6 mutations that results in intellectual impairment and profound short stature. To obtain a better understanding of the genotype-phenotype correlations for PNPLA6-related disorders, we reported the 14th OMCS case and summarized all the reported cases of OMCS. METHODS: We collected clinical biochemical and data and brain MRI data and used whole-exon gene detection and analysis tools to evaluate the pathogenicity of the variants, including PolyPhen-2 and Mutation Taster, and we also generated three-dimensional protein structures and visualized the effects of altered residues with I-TASSER and PyMOL Viewer software. RESULTS: The patient presented with trichomegaly and multiple pituitary hormone deficiencies. Brain MRI showed small pituitary and bilateral paraventricular leukomalacia. Novel variants (c.1491G>T and c.3367G>A) in the PNPLA6 gene were detected in the proband and verified by direct sequencing. Amino acid residues of Gln497 and Gly1123 are predicted to be damaging and destroy the three-dimensional protein structures of the protein. In follow-up, this patient could neither walk nor hold his head erect and had not spoken one word at the age of one year and ten months. Moreover, there is no obvious hot spot mutation in any of the reported allelic variants. Interestingly, the majority of mutations are located in the phospholipid esterase domain, which is responsible for esterase activity. CONCLUSIONS: We identified two novel variants of the PNPLA6 gene in an OMCS patient, which will help to better understand the function of PNPLA6 and genotype-phenotype correlations for PNPLA6-related disorders.
ESTHER : Liu_2020_Gene__145027
PubMedSearch : Liu_2020_Gene__145027
PubMedID: 32758583

Title : Effects and mechanisms of scalp acupuncture on learning and memory in mice with lead poisoning - Yan_2020_J.Pak.Med.Assoc_70 [Special Issue]_125
Author(s) : Yan W , Lou Y , Li G
Ref : J Pak Med Assoc , 70 [Special Issue] :125 , 2020
Abstract : OBJECTIVE: To study the effects and mechanism of scalp acupuncture on learning and memory ability in mice with lead poisoning. METHODS: From March 2018 to December 2018, 30 Kunming mice were randomly divided equally into the control group and the intervention group after intraperitoneal injection of lead acetate The intervention group received scalp acupuncture on the first day of the model establishment; the model group only received conventional feeding without treatment. At the same time, a control group of 15 rats was given the intraperitoneal injections of normal saline for 8 consecutive days, and only after routine feeding, no treatment was given. Determination of lead in blood was detected by Graphite Furnace Atomic Absorption Spectrometry, the Morris water maze test was used to detect the learning and memory function of mice, hydroxylamine colorimetric method was used to measure acetylcholinesterase (AChE) activity, and TUNEL staining was used to detect the apoptotic cells in the hippocampus. RESULTS: The results showed that the blood lead level of the model group (231.42+/-12.53mug/L) was significantly higher than that of the control group (20.43+/-4.62mug/L) (P<0.05); and there was no significant difference in blood lead content between the intervention group (228.12+/-5.21mug/L) and the model group. The Morris water maze test showed that from the fourth day of the orientation navigation experiment, the escape latency of the model group (22.2+/-4.10s) was longer than that of the control group (13.64+/-2.93s) (P<0.05); besides, from the third day, the escape latency of mice in the intervention group (13.52+/-9.18s) was significantly shortened compared with the model group (19.95+/-3.52s). In the space exploration experiment, in terms of passing through the platform, the distance (1.57+/-0.49m) and time (15.54+/-3.72s) of mice in the model group were longer than that of mice in the control group (0.73+/-0.44m, 3.24+/-2.24s) (P<0.05), the distance (0.41+/-0.28m) and time (3.0+/-1.93s) of mice in the intervention group were shorter than that of mice in the model group, and the difference was statistically significant (P<0.05). The apoptosis rate of hippocampus in the model group (8.79+/-0.37%) was significantly higher than that in the control group (3.56+/-0.44%) (P<0.05), and the apoptosis rate of hippocampus in the intervention group (4.36+/-0.12%0 was significantly lower than that in the model group (P<0.05). The expression of AchE in the model group (0.5+/-0.13U/ug) was significantly higher than that in the control group (0.23+/-0.04U/ug), but there was no significant difference in the AChE activity between the intervention group and the model group. CONCLUSIONS: In conclusion, scalp acupuncture can improve the learning and memory ability of mice with lead poisoning, and the decrease of hippocampal apoptotic cells may be a possible mechanism for the improvement of learning and memory function.
ESTHER : Yan_2020_J.Pak.Med.Assoc_70 [Special Issue]_125
PubMedSearch : Yan_2020_J.Pak.Med.Assoc_70 [Special Issue]_125
PubMedID: 33177741

Title : Goodyschle A, a new butenolide with significant BchE inhibitory activity from Goodyera schlechtendaliana - Dai_2020_Nat.Prod.Res__1
Author(s) : Dai LY , Yin QM , Qiu JK , Zhang ZY , Li G , Huang MN , Liu L
Ref : Nat Prod Res , :1 , 2020
Abstract : Goodyschle A (1), a new butenolide, was isolated from the whole grass of Goodyera schlechtendaliana, an orchidaceous edible medicinal plant. The structure of the new compound was elucidated by 1 D and 2 D NMR experiments in addition to HRESIMS analyses. Compound 1 was evaluated for its bioactivities including cytotoxic activity against human gastric cancer (SGC-7901) and human hepatocellular carcinoma (HepG2) cell lines, inhibitory activity on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), and DPPH radical scavenging activity. As a result, compound 1 showed potent BChE inhibitory activity (IC50 value = 6.88 +/- 1.63 muM), moderate DPPH radical scavenging activity (IC50 value = 16.25 +/- 0.21 muM), and slight AChE inhibitory and cytotoxic activities. These findings suggest that compound 1 is worthy for further investigations in terms of its selective BChE inhibitory activity.
ESTHER : Dai_2020_Nat.Prod.Res__1
PubMedSearch : Dai_2020_Nat.Prod.Res__1
PubMedID: 32208851

Title : Identification and functional characterization of a novel heterozygous missense variant in the LPL associated with recurrent hypertriglyceridemia-induced acute pancreatitis in pregnancy - Shi_2020_Mol.Genet.Genomic.Med__e1048
Author(s) : Shi XL , Yang Q , Pu N , Li XY , Chen WW , Zhou J , Li G , Tong ZH , Ferec C , Cooper DN , Chen JM , Li WQ
Ref : Mol Genet Genomic Med , :e1048 , 2020
Abstract : BACKGROUND: Acute pancreatitis in pregnancy (APIP) is a life-threatening disease for both mother and fetus. To date, only three patients with recurrent hypertriglyceridemia-induced APIP (HTG-APIP) have been reported to carry rare variants in the lipoprotein lipase (LPL) gene, which encodes the key enzyme responsible for triglyceride (TG) metabolism. Coincidently, all three patients harbored LPL variants on both alleles and presented with complete or severe LPL deficiency. METHODS: The entire coding regions and splice junctions of LPL and four other TG metabolism genes (APOC2, APOA5, GPIHBP1, and LMF1) were analyzed by Sanger sequencing in a Han Chinese patient who had experienced two episodes of HTG-APIP. The impact of a novel LPL missense variant on LPL protein expression and activity was analyzed by transient expression in HEK293T cells. RESULTS: A novel heterozygous LPL missense variant, p.His210Leu (c.629A > T), was identified in our patient. This variant did not affect protein synthesis but significantly impaired LPL secretion and completely abolished the enzymatic activity of the mutant protein. CONCLUSION: This report describes the first identification and functional characterization of a heterozygous variant in the LPL that predisposed to recurrent HTG-APIP. Our findings confirm a major genetic contribution to the etiology of individual predisposition to HTG-APIP.
ESTHER : Shi_2020_Mol.Genet.Genomic.Med__e1048
PubMedSearch : Shi_2020_Mol.Genet.Genomic.Med__e1048
PubMedID: 31962008
Gene_locus related to this paper: human-LPL

Title : A De Novo Designed Esterase with p-Nitrophenyl Acetate Hydrolysis Activity - Li_2020_Molecules_25_4658
Author(s) : Li G , Xu L , Zhang H , Liu J , Yan J , Yan Y
Ref : Molecules , 25 :4658 , 2020
Abstract : Esterases are a large family of enzymes with wide applications in the industry. However, all esterases originated from natural sources, limiting their use in harsh environments or newly- emerged reactions. In this study, we designed a new esterase to develop a new protocol to satisfy the needs for better biocatalysts. The ideal spatial conformation of the serine catalytic triad and the oxygen anion hole at the substrate-binding site was constructed by quantum mechanical calculation. The catalytic triad and oxygen anion holes were then embedded in the protein scaffold using the new enzyme protocol in Rosetta 3. The design results were subsequently evaluated, and optimized designs were used for expression and purification. The designed esterase had significant lytic activities towards p-nitrophenyl acetate, which was confirmed by point mutations. Thus, this study developed a new protocol to obtain novel enzymes that may be useful in unforgiving environments or novel reactions.
ESTHER : Li_2020_Molecules_25_4658
PubMedSearch : Li_2020_Molecules_25_4658
PubMedID: 33066055
Gene_locus related to this paper: aspor-cutas , fusso-cutas , hevbr-hnl

Title : Multicolor nitrogen dots for rapid detection of thiram and chlorpyrifos in fruit and vegetable samples - Tang_2020_Anal.Chim.Acta_1136_72
Author(s) : Tang Z , Chen Z , Li G , Hu Y
Ref : Anal Chim Acta , 1136 :72 , 2020
Abstract : The development of sensitive fluorescence sensors and efficient preparation of samples is a challenge in the detection of pesticides in complex samples. In this study, multicolor nitrogen dots (M-Ndots) were synthesised via microwave irradiation at 140 degreeC for 10 min with 5-amino-1H-tetrazole and p-phenylenediamine as precursors, which have a high fluorescence quantum yield of up to 31%. Furthermore, the M-Ndots were employed as fluorescence sensors for pesticide detection by being combined with a gas membrane separation device, to eliminate the interference from the complex sample matrix. In this process, the M-Ndots were used for sensing thiram and chlorpyrifos through their affinities to Cu(2+) and Fe(3+), respectively. Because thiram could decompose into volatile CS(2), its derivate was sensed using the fluorescence of M-Ndots via a complexation reaction with Cu(2+). Chlorpyrifos, due to its volatility, can reduce the Fe(3+) ion by inhibiting the activity of acetylcholinesterase, which produces H(2)O(2) to oxidise Fe(2+). In a real application, the time consumption for 96 samples was less than 30 min in one run of the gas membrane separation device. The recoveries for thiram and chlorpyrifos ranged from 90.0% to 115.0%, and the analytical results were validated using LC-MS/MS methods, with relative errors ranging from -7.4% to 10.1%.
ESTHER : Tang_2020_Anal.Chim.Acta_1136_72
PubMedSearch : Tang_2020_Anal.Chim.Acta_1136_72
PubMedID: 33081951

Title : Expression and characterization of an esterase belonging to a new family via isolation from a metagenomic library of paper mill sludge - Jia_2019_Int.J.Biol.Macromol_126_1192
Author(s) : Jia ML , Zhong XL , Lin ZW , Dong BX , Li G
Ref : Int J Biol Macromol , 126 :1192 , 2019
Abstract : A new bacterial lipolytic enzyme Est903 was obtained from paper mill sludge via metagenomic approach. Est903 displayed moderate similarities to two lipolytic enzymes from Rhodopirellula islandica and contained a distinctive pentapeptide motif (GFSAG) that differed from those of all known fourteen families of bacterial lipolytic enzymes. Est903 was regarded as from a new bacterial lipolytic enzyme family through multiple sequence alignment and phylogenetic analysis. The recombinant Est903 showed the highest activity for rho-nitrophenol butyrate. The pH optimum and temperature optimum of the recombinant enzyme was 9.0 and 51 degrees C, respectively. Also, this enzyme displayed moderate thermostability, high activity under alkaline conditions, and good tolerance against several organic solvents. In addition, the compatibility test and washing performance analysis revealed that Est903 had good compatibility with commercial laundry detergent and high cleaning ability of oil stains. These good properties make Est903 a potential candidate in organic synthesis or detergent industry.
ESTHER : Jia_2019_Int.J.Biol.Macromol_126_1192
PubMedSearch : Jia_2019_Int.J.Biol.Macromol_126_1192
PubMedID: 30625356
Gene_locus related to this paper: 9bact-a0a345g0q2

Title : Artificial Nanometalloenzymes for Cooperative Tandem Catalysis - Li_2019_ACS.Appl.Mater.Interfaces_11_15718
Author(s) : Li H , Qiu C , Cao X , Lu Y , Li G , He X , Lu Q , Chen K , Ouyang P , Tan W
Ref : ACS Appl Mater Interfaces , 11 :15718 , 2019
Abstract : Artificial metalloenzymes that combine the advantages of natural enzymes and metal catalysts have been getting more attention in research. As a proof of concept, an artificial nanometalloenzyme (CALB-Shvo@MiMBN) was prepared by co-encapsulation of metallo-organic catalyst and enzyme in a soft nanocomposite consisting of 2-methylimidazole, metal ions, and biosurfactant in mild reaction conditions using a one-pot self-assembly method. The artificial nanometalloenzyme with lipase acted as the core, and the metallo-organic catalyst embedded in micropore exhibited a spherical structure of 30-50 nm in diameter. The artificial nanometalloenzyme showed high catalytic efficiency in the dynamic kinetic resolution of racemic primary amines or secondary alcohols compared to the one-pot catalytic reaction of immobilized lipase and free metallo-organic catalyst. This artificial nanometalloenzyme holds great promise for integrated enzymatic and heterogeneous catalysis.
ESTHER : Li_2019_ACS.Appl.Mater.Interfaces_11_15718
PubMedSearch : Li_2019_ACS.Appl.Mater.Interfaces_11_15718
PubMedID: 30986032

Title : Apelin-13 Suppresses Neuroinflammation Against Cognitive Deficit in a Streptozotocin-Induced Rat Model of Alzheimer's Disease Through Activation of BDNF-TrkB Signaling Pathway - Luo_2019_Front.Pharmacol_10_395
Author(s) : Luo H , Xiang Y , Qu X , Liu H , Liu C , Li G , Han L , Qin X
Ref : Front Pharmacol , 10 :395 , 2019
Abstract : Alzheimer's disease (AD), a progressive neurodegenerative disease characterized by impairments of cognitive function as a result of synaptic deficits and neuronal loss, is associated with inflammation. Apelin-13, a predominant neuropeptide with inhibiting effect on inflammation, has beneficial effects on cognition memory and neuronal damage. However, whether apelin-13 can protect neurons to ameliorate cognitive deficits in AD by inhibiting the inflammatory response remains largely unknown. To test this hypothesis, rats were intracerebroventricularly (ICV) injected with streptozotocin (3 mg/kg) alone or in combination with apelin-13 (2 mug). And tyrosine receptor kinase B (TrkB) blocker K252a (200 nM) was administrated 10 min before apelin injection. Furthermore, cognitive performance was assessed by new object recognition (NOR) and Y-maze tests. Protein expression of apelin, APJ, microglial marker (IBA1), astroglia marker (GFAP), interleukin 1 beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), synaptophysin (SYP), brain-derived neurotrophic factor (BDNF), TrkB, phospho-TrkB (p-TrkB) in the hippocampus were examined by western blotting or immunohistochemistry. And the gene expression of IBA1, GFAP, IL-1beta, TNF-alpha, and SYP were detected by real-time quantitative polymerase chain reaction (PCR). Inflammatory disorder in the hippocampus was tested by hematoxylin and eosin (H&E) staining. The enzyme-linked immunosorbent assay (ELISA) was used to study the expression level of acetylcholine. And the activity of acetylcholinesterase was detected by Acetylcholinesterase Assay Kit. We observed that apelin/APJ signaling was downregulated in the hippocampus of rats administrated with STZ. Apelin-13 was found to significantly ameliorate STZ-induced AD-like phenotypes including congnitive deficit, cholinergic disfunction and the damage of neuron and synaptic plasticity. Moreover, apelin-13 inhibited microglia and astrocyte activation, reduced IL-1beta and TNF-alpha expression and hippocampal BDNF/TrkB expression deficit in AD rats. Finally, apelin-13-mediated effects were blocked by TrkB receptor antagonist K252a. These results suggest that apelin-13 upregulates BDNF/TrkB pathway against cognitive deficit in a STZ-induced rat model of sporadic AD by attenuating inflammation.
ESTHER : Luo_2019_Front.Pharmacol_10_395
PubMedSearch : Luo_2019_Front.Pharmacol_10_395
PubMedID: 31040784

Title : Identification of novel uracil derivatives incorporating benzoic acid moieties as highly potent Dipeptidyl Peptidase-IV inhibitors - Huang_2019_Bioorg.Med.Chem_27_644
Author(s) : Huang J , Deng X , Zhou S , Wang N , Qin Y , Meng L , Li G , Xiong Y , Fan Y , Guo L , Lan D , Xing J , Jiang W , Li Q
Ref : Bioorganic & Medicinal Chemistry , 27 :644 , 2019
Abstract : Dipeptidyl Peptidase-IV (DPP-4) is a validated therapeutic target for type 2 diabetes. Aiming to interact with both residues Try629 and Lys554 in S2' site, a series of novel uracil derivatives 1a-l and 2a-i incorporating benzoic acid moieties at the N3 position were designed and evaluated for their DPP-4 inhibitory activity. Structure-activity relationships (SAR) study led to the identification of the optimal compound 2b as a potent and selective DPP-4 inhibitor (IC50=1.7nM). Docking study revealed the additional salt bridge formed between the carboxylic acid and primary amine of Lys554 has a key role in the enhancement of the activity. Furthermore, compound 2b exhibited no cytotoxicity in human hepatocyte LO2 cells up to 50muM. Subsequent in vivo evaluations revealed that the ester of 2b robustly improves the glucose tolerance in normal mice. The overall results have shown that compound 2b has the potential to a safe and efficacious treatment for T2DM.
ESTHER : Huang_2019_Bioorg.Med.Chem_27_644
PubMedSearch : Huang_2019_Bioorg.Med.Chem_27_644
PubMedID: 30642693

Title : Enhancing the Thermostability of Rhizomucor miehei Lipase with a Limited Screening Library by Rational-Design Point Mutations and Disulfide Bonds - Li_2018_Appl.Environ.Microbiol_84_
Author(s) : Li G , Fang X , Su F , Chen Y , Xu L , Yan Y
Ref : Applied Environmental Microbiology , 84 : , 2018
Abstract : Rhizomucor miehei lipase (RML), as a kind of eukaryotic protein catalyst, plays an important role in the food, organic chemical, and biofuel industries. However, RML retains its catalytic activity below 50 degrees C, which limits its industrial applications at higher temperatures. Soluble expression of this eukaryotic protein in Escherichia coli not only helps to screen for thermostable mutants quickly but also provides the opportunity to develop rapid and effective ways to enhance the thermal stability of eukaryotic proteins. Therefore, in this study, RML was engineered using multiple computational design methods, followed by filtration via conservation analysis and functional region assessment. We successfully obtained a limited screening library (only 36 candidates) to validate thermostable single point mutants, among which 24 of the candidates showed higher thermostability and 13 point mutations resulted in an apparent melting temperature ([Formula: see text]) of at least 1 degrees C higher. Furthermore, both of the two disulfide bonds predicted from four rational-design algorithms were further introduced and found to stabilize RML. The most stable mutant, with T18K/T22I/E230I/S56C-N63C/V189C-D238C mutations, exhibited a 14.3 degrees C-higher [Formula: see text] and a 12.5-fold increase in half-life at 70 degrees C. The catalytic efficiency of the engineered lipase was 39% higher than that of the wild type. The results demonstrate that rationally designed point mutations and disulfide bonds can effectively reduce the number of screened clones to enhance the thermostability of RML.IMPORTANCER. miehei lipase, whose structure is well established, can be widely applied in diverse chemical processes. Soluble expression of R. miehei lipase in E. coli provides an opportunity to explore efficient methods for enhancing eukaryotic protein thermostability. This study highlights a strategy that combines computational algorithms to predict single point mutations and disulfide bonds in RML without losing catalytic activity. Through this strategy, an RML variant with greatly enhanced thermostability was obtained. This study provides a competitive alternative for wild-type RML in practical applications and further a rapid and effective strategy for thermostability engineering.
ESTHER : Li_2018_Appl.Environ.Microbiol_84_
PubMedSearch : Li_2018_Appl.Environ.Microbiol_84_
PubMedID: 29101200
Gene_locus related to this paper: rhimi-lipas

Title : Perfluorododecanoic acid exposure induced developmental neurotoxicity in zebrafish embryos - Guo_2018_Environ.Pollut_241_1018
Author(s) : Guo X , Zhang S , Lu S , Zheng B , Xie P , Chen J , Li G , Liu C , Wu Q , Cheng H , Sang N
Ref : Environ Pollut , 241 :1018 , 2018
Abstract : Perfluorododecanoic acid (PFDoA), an artificial perfluorochemical, has been widely distributed in different ambient media and has been reported to have the potential to cause developmental neurotoxicity. However, the specific mechanism is largely unknown. In the current study, zebrafish embryos were treated with 0, 0.24, 1.2, and 6mg/L PFDoA for 120h. Exposure to PFDoA causes serious decreases in hatching delay, body length, as well as decreased locomotor speed in zebrafish larvae. Additionally, the acetylcholine (ACh) content as well as acetylcholinesterase (AChE) activity were determined to be significantly downregulated in PFDoA treatment groups. The level of dopamine was upregulated significantly after treating with 1.2 and 6mg/L of PFDoA. Gene expressions related to the nervous system development were also analyzed, with the exception of the gene mesencephalic astrocyte-derived neurotrophic factor (manf), which is upregulated in the 6mg/L treatment group. All other genes were significantly downregulated in larvae in the PFDoA group in different degrees. In general, the results demonstrated that PFDoA exposure could result in the disruption of the cholinergic system, dopaminergic signaling, and the central nervous system.
ESTHER : Guo_2018_Environ.Pollut_241_1018
PubMedSearch : Guo_2018_Environ.Pollut_241_1018
PubMedID: 30029309

Title : Synthesis and activity towards Alzheimer's disease in vitro: Tacrine, phenolic acid and ligustrazine hybrids - Li_2018_Eur.J.Med.Chem_148_238
Author(s) : Li G , Hong G , Li X , Zhang Y , Xu Z , Mao L , Feng X , Liu T
Ref : Eur Journal of Medicinal Chemistry , 148 :238 , 2018
Abstract : A series of novel tacrine-phenolic acid dihybrids and tacrine-phenolic acid-ligustrazine trihybrids were synthesized, characterized and screened as novel potential anti-Alzheimer drug candidates. These compounds showed potent inhibition activity towards cholinesterases (ChEs), among of them, 9i was the most potent one towards acetylcholinesterase (eeAChE, IC50=3.9nM; hAChE, IC50=65.2nM). 9i could also effectively block beta-amyloid (Abeta) self-aggregation with an inhibition ratio of 47% at 20muM. In addition, its strong anti-oxidation activity could protect PC12cells from CoCl2-damage in the experimental condition while no neurotoxicity. Furthermore, its hepatotoxicity was lower than tacrine in vitro and in vivo. Kinetic and molecular modeling studies revealed that 9i worked in a mixed-type way, could interact simultaneously with catalytic active site (CAS) and peripheral anionic site (PAS) of AChE. Therefore, 9i was a promising multifunctional candidate for the treatment of AD.
ESTHER : Li_2018_Eur.J.Med.Chem_148_238
PubMedSearch : Li_2018_Eur.J.Med.Chem_148_238
PubMedID: 29466774

Title : Alogliptin prevents diastolic dysfunction and preserves left ventricular mitochondrial function in diabetic rabbits - Zhang_2018_Cardiovasc.Diabetol_17_160
Author(s) : Zhang X , Zhang Z , Yang Y , Suo Y , Liu R , Qiu J , Zhao Y , Jiang N , Liu C , Tse G , Li G , Liu T
Ref : Cardiovasc Diabetol , 17 :160 , 2018
Abstract : BACKGROUND: There are increasing evidence that left ventricle diastolic dysfunction is the initial functional alteration in the diabetic myocardium. In this study, we hypothesized that alogliptin prevents diastolic dysfunction and preserves left ventricular mitochondrial function and structure in diabetic rabbits. METHODS: A total of 30 rabbits were randomized into control group (CON, n = 10), alloxan-induced diabetic group (DM, n = 10) and alogliptin-treated (12.5 mg/kd/day for 12 weeks) diabetic group (DM-A, n = 10). Echocardiographic and hemodynamic studies were performed in vivo. Mitochondrial morphology, respiratory function, membrane potential and reactive oxygen species (ROS) generation rate of left ventricular tissue were assessed. The serum concentrations of glucagon-like peptide-1, insulin, inflammatory and oxidative stress markers were measured. Protein expression of TGF-beta1, NF-kappaB p65 and mitochondrial biogenesis related proteins were determined by Western blotting. RESULTS: DM rabbits exhibited left ventricular hypertrophy, left atrial dilation, increased E/e' ratio and normal left ventricular ejection fraction. Elevated left ventricular end diastolic pressure combined with decreased maximal decreasing rate of left intraventricular pressure (- dp/dtmax) were observed. Alogliptin alleviated ventricular hypertrophy, interstitial fibrosis and diastolic dysfunction in diabetic rabbits. These changes were associated with decreased mitochondrial ROS production rate, prevented mitochondrial membrane depolarization and improved mitochondrial swelling. It also improved mitochondrial biogenesis by PGC-1alpha/NRF1/Tfam signaling pathway. CONCLUSIONS: The DPP-4 inhibitor alogliptin prevents cardiac diastolic dysfunction by inhibiting ventricular remodeling, explicable by improved mitochondrial function and increased mitochondrial biogenesis.
ESTHER : Zhang_2018_Cardiovasc.Diabetol_17_160
PubMedSearch : Zhang_2018_Cardiovasc.Diabetol_17_160
PubMedID: 30591063

Title : A machine learning approach for reliable prediction of amino acid interactions and its application in the directed evolution of enantioselective enzymes - Cadet_2018_Sci.Rep_8_16757
Author(s) : Cadet F , Fontaine N , Li G , Sanchis J , Ng Fuk Chong M , Pandjaitan R , Vetrivel I , Offmann B , Reetz MT
Ref : Sci Rep , 8 :16757 , 2018
Abstract : Directed evolution is an important research activity in synthetic biology and biotechnology. Numerous reports describe the application of tedious mutation/screening cycles for the improvement of proteins. Recently, knowledge-based approaches have facilitated the prediction of protein properties and the identification of improved mutants. However, epistatic phenomena constitute an obstacle which can impair the predictions in protein engineering. We present an innovative sequence-activity relationship (innov'SAR) methodology based on digital signal processing combining wet-lab experimentation and computational protein design. In our machine learning approach, a predictive model is developed to find the resulting property of the protein when the n single point mutations are permuted (2(n) combinations). The originality of our approach is that only sequence information and the fitness of mutants measured in the wet-lab are needed to build models. We illustrate the application of the approach in the case of improving the enantioselectivity of an epoxide hydrolase from Aspergillus niger. n = 9 single point mutants of the enzyme were experimentally assessed for their enantioselectivity and used as a learning dataset to build a model. Based on combinations of the 9 single point mutations (2(9)), the enantioselectivity of these 512 variants were predicted, and candidates were experimentally checked: better mutants with higher enantioselectivity were indeed found.
ESTHER : Cadet_2018_Sci.Rep_8_16757
PubMedSearch : Cadet_2018_Sci.Rep_8_16757
PubMedID: 30425279

Title : Association of genetic polymorphisms of telomere binding proteins with cholinesterase activity in omethoate-exposed workers - Ding_2018_Ecotoxicol.Environ.Saf_161_563
Author(s) : Ding M , Yang Y , Duan X , Wang S , Feng X , Wang T , Wang P , Liu S , Li L , Liu J , Tang L , Niu X , Zhang Y , Li G , Yao W , Cui L , Wang W
Ref : Ecotoxicology & Environmental Safety , 161 :563 , 2018
Abstract : Omethoate, an organophosphorous pesticide, can cause a variety of health effects, especially the decrease of cholinesterase activity. The aim of this study is to explore the association of genetic polymorphisms of telomere binding proteins with cholinesterase activity in omethoate-exposed population. Cholinesterase activities in whole blood, red blood cell and plasma were detected using acetylthiocholine and dithio-bis-(nitrobenzoic acid) method; Genetic Genotyping of POT1 rs1034794, POT1 rs10250202, TERF1 rs3863242 and TERT rs2736098 were performed with PCR-RFLP. The cholinesterase activities of whole blood, red blood cells and plasma in exposure group are significantly lower than that of the control group (P<0.001). Multivariate analysis indicates that exposure group (b=-1.016, P<0.001), agender (b=0.365, P<0.001), drinking (b=0.271, P=0.004) and TERF1rs3863242 (b=-0.368, P=0.016) had an impact on cholinesterase activities. The results suggest that individual carrying AG+GG genotypes in TERF1 gene rs3863242 polymorphism were susceptible to damage in cholinesterase induced by omethoate.
ESTHER : Ding_2018_Ecotoxicol.Environ.Saf_161_563
PubMedSearch : Ding_2018_Ecotoxicol.Environ.Saf_161_563
PubMedID: 29929132

Title : TaEDS1 genes positively regulate resistance to powdery mildew in wheat - Chen_2018_Plant.Mol.Biol_96_607
Author(s) : Chen G , Wei B , Li G , Gong C , Fan R , Zhang X
Ref : Plant Mol Biol , 96 :607 , 2018
Abstract : KEY MESSAGE: Three EDS1 genes were cloned from common wheat and were demonstrated to positively regulate resistance to powdery mildew in wheat. The EDS1 proteins play important roles in plant basal resistance and TIR-NB-LRR protein-triggered resistance in dicots. Until now, there have been very few studies on EDS1 in monocots, and none in wheat. Here, we report on three common wheat orthologous genes of EDS1 family (TaEDS1-5A, 5B and 5D) and their function in powdery mildew resistance. Comparisons of these genes with their orthologs in diploid ancestors revealed that EDS1 is a conserved gene family in Triticeae. The cDNA sequence similarity among the three TaEDS1 genes was greater than 96.5%, and they shared sequence similarities of more than 99.6% with the respective orthologs from diploid ancestors. The phylogenetic analysis revealed that the EDS1 family originated prior to the differentiation of monocots and dicots, and EDS1 members have since undergone clear structural differentiation. The transcriptional levels of TaEDS1 genes in the leaves were obviously higher than those of the other organs, and they were induced by Blumeria graminis f. sp. tritici (Bgt) infection and salicylic acid (SA) treatment. The BSMV-VIGS experiments indicated that knock-down the transcriptional levels of the TaEDS1 genes in a powdery mildew-resistant variety of common wheat compromised resistance. Contrarily, transient overexpression of TaEDS1 genes in a susceptible common wheat variety significantly reduced the haustorium index and attenuated the growth of Bgt. Furthermore, the expression of TaEDS1 genes in the Arabidopsis mutant eds1-1 complemented its susceptible phenotype to powdery mildew. The above evidences strongly suggest that TaEDS1 acts as a positive regulator and confers resistance against powdery mildew in common wheat.
ESTHER : Chen_2018_Plant.Mol.Biol_96_607
PubMedSearch : Chen_2018_Plant.Mol.Biol_96_607
PubMedID: 29582247
Gene_locus related to this paper: wheat-a0a1d5zte7

Title : Z-Ligustilide Exerted Hormetic Effect on Growth and Detoxification Enzymes of Spodoptera litura Larvae - Yi_2018_Evid.Based.Complement.Alternat.Med_2018_7104513
Author(s) : Yi Y , Dou G , Yu Z , He H , Wang C , Li L , Zhou J , Liu D , Shi J , Li G , Pang L , Yang N , Huang Q , Qi H
Ref : Evid Based Complement Alternat Med , 2018 :7104513 , 2018
Abstract : Plants have evolved a variety of phytochemicals to defense insect feeding, whereas insects have also evolved diverse detoxification enzymes, which are adaptively induced as a prosurvival mechanism. Herein, Z-ligustilide in Ligusticum chuanxiong Hort. was found to exhibit a similar trend in the accumulation from December to May as the occurrence of Spodoptera litura (Fabricius) larvae. Importantly, S. litura larvae feeding enhanced Z-ligustilide level in the stem and leaf (p < 0.01). Moreover, Z-ligustilide ranging from 1 to 5 mg.g(-1) exhibited remarkable larvicidal activity, antifeedant activity, and growth inhibition against S. litura larvae. The LC50 values of larvicidal activity for phthalides in L. chuanxiong were compared as follows: Z-ligustilide > levistilide A > senkyunolide A > 3-butylidenephthalide > senkyunolide I, implicating the critical role of conjugated structure. Notably, there was a biphasic dose response for glutathione S-transferase (GST), cytochrome P450 (CYP) 450, Acetylcholinesterase (AChE), and Carboxylesterase (CarE) activities and GSTs1, cytochrome P450 (CYP) 4S9, and CYP4M14 mRNA expression. Particularly, low dose (0.1 mg.g(-1)) of Z-ligustilide conferred the resistance of S. litura larvae against chlorpyrifos (p < 0.05). Together, our data suggest that Z-ligustilide may function in a hormetic way in the chemical defense of L. chuanxiong against S. litura larvae.
ESTHER : Yi_2018_Evid.Based.Complement.Alternat.Med_2018_7104513
PubMedSearch : Yi_2018_Evid.Based.Complement.Alternat.Med_2018_7104513
PubMedID: 30057645

Title : The dual DPP4 inhibitor and GPR119 agonist HBK001 regulates glycemic control and beta cell function ex and in vivo - Huan_2017_Sci.Rep_7_4351
Author(s) : Huan Y , Jiang Q , Li G , Bai G , Zhou T , Liu S , Li C , Liu Q , Sun S , Yang M , Guo N , Wang X , Wang S , Liu Y , Wang G , Huang H , Shen Z
Ref : Sci Rep , 7 :4351 , 2017
Abstract : Glucagon like peptide-1 (GLP-1) plays a vital role in glucose homeostasis and sustaining beta-cell function. Currently there are two major methods to enhance endogenous GLP-1 activity; inhibiting dipeptidyl peptidase-4 (DPP4) or activating G protein-coupled receptor 119 (GPR119). Here we describe and validate a novel dual-target compound, HBK001, which can both inhibit DPP4 and activate GPR119 ex and in vivo. We show that HBK001 can promote glucose-stimulated insulin secretion in mouse and human primary islets. A single administration of HBK001 in ICR mice can increase plasma incretins levels much more efficiently than linagliptin, a classic DPP4 inhibitor. Long-term treatment of HBK001 in KKAy mice can ameliorate hyperglycemia as well as improve glucose tolerance, while linagliptin fails to achieve such glucose-lowing effects despite inhibiting 95% of serum DPP4 activity. Moreover, HBK001 can increase first-phase insulin secretion in KKAy mice, suggesting a direct effect on islet beta-cells via GPR119 activation. Furthermore, HBK001 can improve islet morphology, increase beta-cell proliferation and up-regulate genes involved in improved beta-cell function. Thus, we have identified, designed and synthesized a novel dual-target compound, HBK001, which represents a promising therapeutic candidate for type 2 diabetes, especially for patients who are insensitive to current DPP4 inhibitors.
ESTHER : Huan_2017_Sci.Rep_7_4351
PubMedSearch : Huan_2017_Sci.Rep_7_4351
PubMedID: 28659588

Title : New diterpenoids isolated from Leonurus japonicus and their acetylcholinesterase inhibitory activity - Wu_2017_Chin.J.Nat.Med_15_860
Author(s) : Wu HK , Sun T , Zhao F , Zhang LP , Li G , Zhang J
Ref : Chin J Nat Med , 15 :860 , 2017
Abstract : Three new labdane diterpenoids, leojaponicone A (1), isoleojaponicone A (2) and methylisoleojaponicone A (3), were isolated from the herb of Leonurus japonicus. The chemical structures of these secondary metabolites were elucidated on the basis of 1D and 2D NMR, including HMQC, and HMBC spectroscopic techniques. All the new compounds were tested in vitro for their acetylcholinesterase and alpha-glucosidase inhibitory activity. Compounds 1-3 exhibited low inhibitory effects on alpha-glucosidase with respect to acarbose and exhibited high inhibitory effects on acetylcholinesterase with respect to huperzine A.
ESTHER : Wu_2017_Chin.J.Nat.Med_15_860
PubMedSearch : Wu_2017_Chin.J.Nat.Med_15_860
PubMedID: 29329613

Title : Parental transfer of microcystin-LR induced transgenerational effects of developmental neurotoxicity in zebrafish offspring - Wu_2017_Environ.Pollut_231_471
Author(s) : Wu Q , Yan W , Cheng H , Liu C , Hung TC , Guo X , Li G
Ref : Environ Pollut , 231 :471 , 2017
Abstract : Microcystin-LR (MCLR) has been reported to cause developmental neurotoxicity in zebrafish, but there are few studies on the mechanisms of MCLR-induced transgenerational effects of developmental neurotoxicity. In this study, zebrafish were exposed to 0, 1, 5, and 25 mug/L MCLR for 60 days. The F1 zebrafish embryos from the above-mentioned parents were collected and incubated in clean water for 120 h for hatching. After examining the parental zebrafish and F1 embryos, MCLR was detected in the gonad of adults and F1 embryos, indicating MCLR could potentially be transferred from parents to offspring. The larvae also showed a serious hypoactivity. The contents of dopamine, dihydroxyphenylacetic acid (DOPAC), serotonin, gamma-aminobutyric acid (GABA) and acetylcholine (ACh) were further detected, but only the first three neurotransmitters showed significant reduction in the 5 and 25 mug/L MCLR parental exposure groups. In addition, the acetylcholinesterase (AChE) activity was remarkably decreased in MCLR parental exposure groups, while the expression levels of manf, bdnf, ache, htr1ab, htr1b, htr2a, htr1aa, htr5a, DAT, TH1 and TH2 genes coincided with the decreased content of neurotransmitters (dopamine, DOPAC and serotonin) and the activity of AChE. Neuronal development related genes, alpha1-tubulin, syn2a, mbp, gfap, elavl3, shha and gap43 were also measured, but gap43 was the gene only up-regulated. Our results demonstrated MCLR could be transferred to offspring, and subsequently induce developmental neurotoxicity in F1 zebrafish larvae by disturbing the neurotransmitter systems and neuronal development.
ESTHER : Wu_2017_Environ.Pollut_231_471
PubMedSearch : Wu_2017_Environ.Pollut_231_471
PubMedID: 28837927

Title : Tris(1,3-dichloro-2-propyl) phosphate disrupts axonal growth, cholinergic system and motor behavior in early life zebrafish - Cheng_2017_Aquat.Toxicol_192_7
Author(s) : Cheng R , Jia Y , Dai L , Liu C , Wang J , Li G , Yu L
Ref : Aquat Toxicol , 192 :7 , 2017
Abstract : Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) could have neurotoxic effects and alter motor behaviors in zebrafish (Danio rerio) larvae, however, the underlying mechanisms are still unknown. In this study, zebrafish embryos were subjected to waterborne exposure of TDCIPP at 100, 300, 600, 900mug/L from 2 to 120-h post-fertilization (hpf). Behavioral measurements indicate that TDCIPP exposure significantly elevated spontaneous movement, and altered swimming behavior response of larvae to both light and dark stimulation. Interestingly, in accordance with these motor effects, TDCIPP significantly decreased expression of the neuron-specific GFP in transgenic (HuC-GFP) zebrafish larvae as well as decreased expression of the neural marker genes elavl3 and ngn1, inhibited the axonal growth of the secondary motoneurons and altered the expressions of axon-related genes (alpha1-tubulin, shha and netrin2) in zebrafish larvae. Furthermore, TDCIPP exposure at 900mug/L significantly increased the activity of acetylcholinesterase (AChE) enzyme, and decreased the total acetylcholine (ACh) concentration. Our data indicate that the alteration in motor neuron and inhibition of cholinergic system could together lead to the TDCIPP induced motor behavior alterations in zebrafish larvae.
ESTHER : Cheng_2017_Aquat.Toxicol_192_7
PubMedSearch : Cheng_2017_Aquat.Toxicol_192_7
PubMedID: 28898785

Title : Preventative effects of fermented Chimonobambusa quadrangularis shoot on activated carbon-induced constipation - Li_2017_Exp.Ther.Med_13_1093
Author(s) : Li G , Zou X , Kuang G , Ren Y , Deng C , Lin Q , Zhao X , Xu S , Song JL
Ref : Exp Ther Med , 13 :1093 , 2017
Abstract : The present study aimed to determine the preventative effects of fermented Chimonobambusa quadrangularis shoot (FCQS) on activated carbon constipation in Kun Ming mice. FCQS has a more loose fiber tissue structure than unfermented fresh C. quadrangularis shoot (CQS), which is preferable for relieving constipation. In mice fed with FCQS for 9 days the time from consumption to their first black stool defecation (117 min) was shorter than the control group (192 min) and the CQS group (148 min); however, it was longer than the normal (85 min) and bisacodyl treatment (99 min) groups. The gastrointestinal transit of the FCQS group (73.8%) was increased, as compared with the control (37.9%) and CQS (61.7%) groups; however, it was decreased as compared with the normal (100%) and bisacodyl (88.3%) groups. By observing the hemotoxylin and eosin-stained section of mice intestine, it was demonstrated that FCQS reduced injury to the intestinal tract resulting from constipation and alleviated the damage caused to the intestinal villi over the effects observed in the CQS group. Furthermore, FCQS was also able to increase the serum levels of motilin, endothelin-1, vasoactive intestinal peptide and acetylcholinesterase compared with the control group. c-Kit, stem cell factor (SCF), glial cell-derived neurotrophic factor (GDNF) mRNA and protein expression levels in the small intestinal cells of FCQS-fed mice were increased, as compared with CQS-fed mice. Transient receptor potential cation channel subfamily V member 1 (TRPV1) and nitric oxide synthase (NOS) expression levels of small intestinal cells of FCQS-fed mice were reduced, as compared with CQS-fed mice. These findings demonstrated that FCQS may induce improved preventative effects on constipation, compared with CQS.
ESTHER : Li_2017_Exp.Ther.Med_13_1093
PubMedSearch : Li_2017_Exp.Ther.Med_13_1093
PubMedID: 28450948

Title : Nonenzymatic all-solid-state coated wire electrode for acetylcholine determination in vitro - He_2016_Biosens.Bioelectron_85_679
Author(s) : He C , Wang Z , Wang Y , Hu R , Li G
Ref : Biosensors & Bioelectronics , 85 :679 , 2016
Abstract : A nonenzymatic all-solid-state coated wire acetylcholine electrode was investigated. Poly(3,4-ethylenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT/PSS) as conducting polymer was coated on one end of a gold wire (0.5mm in diameter). The acetylcholine selective membrane containing heptakis(2,3,6-tri-Omicron-methyl)-beta-cyclodextrin as an ionophore covered the conducting polymer layer. The electrode could work stably in a pH range of 6.5-8.5 and a temperature range of 15-40 degrees C. It covered an acetylcholine concentration range of 10(-5)-10(-1)M with a slope of 54.04+/-1.70mV/decade, while detection limit was 5.69+/-1.06microM. The selectivity, dynamic response, reproducibility and stability were evaluated. The electrode could work properly in the rat brain homogenate to detect different concentrations of acetylcholine.
ESTHER : He_2016_Biosens.Bioelectron_85_679
PubMedSearch : He_2016_Biosens.Bioelectron_85_679
PubMedID: 27254787

Title : Galantamine protects against lipopolysaccharide-induced acute lung injury in rats - Li_2016_Braz.J.Med.Biol.Res_49_
Author(s) : Li G , Zhou C , Zhou Q , Zou H
Ref : Brazilian Journal of Medical & Biological Research , 49 : , 2016
Abstract : Lipopolysaccharide (LPS)-induced endotoxemia triggers the secretion of proinflammatory cytokines and can cause acute lung injury (ALI). The high mobility group box 1 (HMGB1) protein plays an important role as a late mediator of sepsis and ALI. Galantamine (GAL) is a central acetylcholinesterase inhibitor that inhibits the expression of HMGB1. This study evaluated the effects of GAL by measuring levels of inflammatory mediators and observing histopathological features associated with LPS-induced ALI. Sixty 8-10 week old male Sprague-Dawley rats (200-240 g) were randomized into three groups as follows: control group, LPS group (7.5 mg/kg LPS), and LPS+GAL group (5 mg/kg GAL before LPS administration). Histopathological examination of lung specimens obtained 12 h after LPS administration was performed to analyze changes in wet-to-dry (W/D) weight ratio, myeloperoxidase (MPO) activity, and HMGB1 expression level. Additionally, plasma concentrations of tumor necrosis factor-alpha, interleukin-6, and HMGB1 were measured using an enzyme-linked immunosorbent assay at 0 (baseline), 3, 6, 9, and 12 h after LPS administration. Mortality in the three groups was recorded at 72 h. LPS-induced ALI was characterized by distortion of pulmonary architecture and elevation of MPO activity, W/D weight ratio, and levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha, interleukin-6, and HMGB1. Pretreatment with GAL significantly reduced the LPS-induced lung pathological changes, W/D weight ratio, levels of pro-inflammatory cytokines and MPO activity (ANOVA). Moreover, GAL treatment significantly decreased the mortality rate (ANOVA). In conclusion, we demonstrated that GAL exerted a protective effect on LPS-induced ALI in rats.
ESTHER : Li_2016_Braz.J.Med.Biol.Res_49_
PubMedSearch : Li_2016_Braz.J.Med.Biol.Res_49_
PubMedID: 26648090

Title : Sublethal Effects of Insecticide Exposure on Megacopta cribraria (Fabricius) Nymphs: Key Biological Traits and Acetylcholinesterase Activity - Miao_2016_J.Insect.Sci_16_
Author(s) : Miao J , Reisig DD , Li G , Wu Y
Ref : J Insect Sci , 16 : , 2016
Abstract : Megacopta cribraria F. (Hemiptera: Plataspidae), the kudzu bug, is an invasive insect pest of U.S. soybean. At present, insecticide application is the primary and most effective control option for M. cribraria In this study, the potential effects of sublethal and low-lethal concentrations (LC10 and LC40) of three common insecticides on key biological traits and acetylcholinesterase (AChE) activity of the treated nymphal stage of insect were assessed. The results show that the sublethal concentration of imidacloprid significantly reduced adult emergence rate of M. cribraria A low-lethal concentration of imidacloprid significantly increased nymphal development time, but significantly decreased adult emergence rate and adult longevity. Both sublethal and low-lethal concentrations of acephate caused an increase in nymphal development time and a reduction in adult emergence rate and adult longevity. Fecundity of females was significantly reduced only by exposure to low-lethal concentrations of acephate. Sublethal and low-lethal concentrations of bifenthrin increased nymphal development time, but significantly decreased adult emergence rate. In addition, we found that the AChE activity of M. cribraria was significantly increased only by LC40 imidacloprid, but strongly inhibited by acephate.
ESTHER : Miao_2016_J.Insect.Sci_16_
PubMedSearch : Miao_2016_J.Insect.Sci_16_
PubMedID: 27638957

Title : Enhanced performance of lipase via microcapsulation and its application in biodiesel preparation - Su_2016_Sci.Rep_6_29670
Author(s) : Su F , Li G , Fan Y , Yan Y
Ref : Sci Rep , 6 :29670 , 2016
Abstract : In the present study, a surface-active enzyme, lipase was immobilized in polyethyleneimine (PEI) microcapsules and then modified with oxidized multiwall carbon nanotubes (MWCNTs). The resulting lipase microcapsules exhibited higher activity and stability, since the activity of microcapsules was 21.9 folds than that of the free counterpart. Numerous interfaces which were created in polycondensation enhanced the performance of lipases. Illustrated by confocal laser scanning microscope (CLSM), it was found that microcapsules, whose barrier properties against molecules with diameter >4.6 nm, were with a semipermeable and porous membrane structure. The lipases preferred to locate in the wall of the microcapsules. The oxidized multiwall carbon nanotubes (MWCNTs) were further added to modify microcapsules, resulting in even higher activity. The nanocomposites were examined by scanning electron microscope (SEM) and zeta-potential analyzer. The results indicated the superior catalytic performances were attributed to the augmented interface and decreased positive charge. Finally, the MWCNTs modified microcapsules were utilized in producing biodiesel with a 97.15% yield and retained nearly 90% yield after running 10 cycles. This approach of microcapsulation will be highly beneficial for preparing various bio-active microcapsules with excellent catalytic performance.
ESTHER : Su_2016_Sci.Rep_6_29670
PubMedSearch : Su_2016_Sci.Rep_6_29670
PubMedID: 27424490

Title : Bacterial cellulose synthesis mechanism of facultative anaerobe Enterobacter sp. FY-07 - Ji_2016_Sci.Rep_6_21863
Author(s) : Ji K , Wang W , Zeng B , Chen S , Zhao Q , Chen Y , Li G , Ma T
Ref : Sci Rep , 6 :21863 , 2016
Abstract : Enterobacter sp. FY-07 can produce bacterial cellulose (BC) under aerobic and anaerobic conditions. Three potential BC synthesis gene clusters (bcsI, bcsII and bcsIII) of Enterobacter sp. FY-07 have been predicted using genome sequencing and comparative genome analysis, in which bcsIII was confirmed as the main contributor to BC synthesis by gene knockout and functional reconstitution methods. Protein homology, gene arrangement and gene constitution analysis indicated that bcsIII had high identity to the bcsI operon of Enterobacter sp. 638; however, its arrangement and composition were same as those of BC synthesizing operon of G. xylinum ATCC53582 except for the flanking sequences. According to the BC biosynthesizing process, oxygen is not directly involved in the reactions of BC synthesis, however, energy is required to activate intermediate metabolites and synthesize the activator, c-di-GMP. Comparative transcriptome and metabolite quantitative analysis demonstrated that under anaerobic conditions genes involved in the TCA cycle were downregulated, however, genes in the nitrate reduction and gluconeogenesis pathways were upregulated, especially, genes in three pyruvate metabolism pathways. These results suggested that Enterobacter sp. FY-07 could produce energy efficiently under anaerobic conditions to meet the requirement of BC biosynthesis.
ESTHER : Ji_2016_Sci.Rep_6_21863
PubMedSearch : Ji_2016_Sci.Rep_6_21863
PubMedID: 26911736
Gene_locus related to this paper: 9entr-a0a127l3e1

Title : Microcystin-LR exposure induces developmental neurotoxicity in zebrafish embryo - Wu_2016_Environ.Pollut_213_793
Author(s) : Wu Q , Yan W , Liu C , Li L , Yu L , Zhao S , Li G
Ref : Environ Pollut , 213 :793 , 2016
Abstract : Microcystin-LR (MCLR) is a commonly acting potent hepatotoxin and has been pointed out of potentially causing developmental neurotoxicity, but the exact mechanism is little known. In this study, zebrafish embryos were exposed to 0, 0.8, 1.6 or 3.2 mg/L MCLR for 120 h. MCLR exposure through submersion caused serious hatching delay and body length decrease. The content of MCLR in zebrafish larvae was analyzed and the results demonstrated that MCLR can accumulate in zebrafish larvae. The locomotor speed of zebrafish larvae was decreased. Furthermore, the dopamine and acetylcholine (ACh) content were detected to be significantly decreased in MCLR exposure groups. And the acetylcholinesterase (AChE) activity was significantly increased after exposure to 1.6 and 3.2 mg/L MCLR. The transcription pattern of manf, chrnalpha7 and ache gene was consistent with the change of the dopamine content, ACh content and AChE activity. Gene expression involved in the development of neurons was also measured. a1-tubulin and shha gene expression were down-regulated, whereas mbp and gap43 gene expression were observed to be significantly up-regulated upon exposure to MCLR. The above results indicated that MCLR-induced developmental toxicity might attribute to the disorder of cholinergic system, dopaminergic signaling, and the development of neurons.
ESTHER : Wu_2016_Environ.Pollut_213_793
PubMedSearch : Wu_2016_Environ.Pollut_213_793
PubMedID: 27038211

Title : Increased serum level of Lp-PLA2 is independently associated with the severity of coronary artery diseases: a cross-sectional study of Chinese population - Cai_2015_BMC.Cardiovasc.Disord_15_14
Author(s) : Cai A , Li G , Chen J , Li X , Li L , Zhou Y
Ref : BMC Cardiovasc Disord , 15 :14 , 2015
Abstract : BACKGROUND: Lipoprotein-associated phospholipase A2 (Lp-PLA2) plays complex and adverse roles on atherosclerosis. Current study was to investigate whether increased plasma Lp-PLA2 level is independently associated with the severity of coronary artery diseases (CAD).
METHODS: Totally 781 participants were enrolled and performed coronary angiography (CAG) to figure out the number of coronary artery stenosis. According to clinical presentation, electrocardiography, cardiac biomarker, and CAG result, participants were divided into control (excluded CAD), stable angina (SA), unstable angina (UA) and acute myocardial infarction (AMI) groups. Baseline characteristics were recorded. Statistical analyses were performed to evaluate the relationship between Lp-PLA2 level and CAD severity.
RESULTS: Plasma levels of Lp-PLA2 in control, SA, UA and AMI groups were 7.38(3.33-9.26) mug/L, 5.94(2.89-8.55) mug/L, 8.56(5.34-11.95) mug/L and 8.68(5.56-13.27) mug/L respectively (P < 0.001). After adjusted for age, gender, smoking, diabetes mellitus, hypertension, low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), apoprotein A (apoA) and statins, Lp-PLA2 level was still independently associated with CAD severity, with odd ratio (OR) of 1.055 (AMI group versus control group, 95% confidence interval (CI) 1.021-1.090, P < 0.05). Additionally, the relationship between Lp-PLA2 level and the number of stenosis coronary artery was also assessed. Lp-PLA2 levels in control, single-vessel, and multiple-vessels stenosis groups were 7.38(3.33-9.26) mug/L, 7.80 (4.05-10.76) mug/L and 8.29(5.18-11.76) mug/L respectively (P for trend < 0.001). After adjusted for age, gender, smoking, diabetes mellitus, hypertension, LDL-C and HDL-C, apoA and statins, Lp-PLA2 level remained independently associated with the number of coronary artery stenosis, with OR of 1.053 (multiple-vessels stenosis group versus control group, 95% CI 1.025-1.069, P < 0.05). CONCLUSION: Increased Lp-PLA2 level is independently associated with CAD severity, and Lp-PLA2 level may be used to discriminate those who are at increased risk of cardiovascular events.
ESTHER : Cai_2015_BMC.Cardiovasc.Disord_15_14
PubMedSearch : Cai_2015_BMC.Cardiovasc.Disord_15_14
PubMedID: 25879827

Title : Cholinergic activation of the murine trachealis muscle via non-vesicular acetylcholine release involving low-affinity choline transporters - Nassenstein_2015_Int.Immunopharmacol_29(1)_173
Author(s) : Nassenstein C , Wiegand S , Lips KS , Li G , Klein J , Kummer W
Ref : Int Immunopharmacol , 29 :173 , 2015
Abstract : In addition to quantal, vesicular release of acetylcholine (ACh), there is also non-quantal release at the motor endplate which is insufficient to evoke postsynaptic responses unless acetylcholinesterase (AChE) is inhibited. We here addressed potential non-quantal release in the mouse trachea by organ bath experiments and (immuno)histochemical methods. Electrical field stimulation (EFS) of nerve terminals elicited tracheal constriction that is largely due to ACh release. Classical enzyme histochemistry demonstrated acetylcholinesterase (AChE) activity in nerve fibers in the muscle and butyrylcholinesterase (BChE) activity in the smooth muscle cells. Acute inhibition of both esterases by eserine significantly raised tracheal tone which was fully sensitive to atropine. This effect was reduced, but not abolished, in AChE, but not in BChE gene-deficient mice. The eserine-induced increase in tracheal tone was unaffected by vesamicol (10(-5)M), an inhibitor of the vesicular acetylcholine transporter, and by corticosterone (10(-4)M), an inhibitor of organic cation transporters. Hemicholinium-3, in low concentrations an inhibitor of the high-affinity choline transporter-1 (CHT1), completely abrogated the eserine effects when applied in high concentrations (10(-4)M) pointing towards an involvement of low-affinity choline transporters. To evaluate the cellular sources of non-quantal ACh release in the trachea, expression of low-affinity choline transporter-like family (CTL1-5) was evaluated by RT-PCR analysis. Even though these transporters were largely abundant in the epithelium, denudation of airway epithelial cells had no effect on eserine-induced tracheal contraction, indicating a non-quantal release of ACh from non-epithelial sources in the airways. These data provide evidence for an epithelium-independent non-vesicular, non-quantal ACh release in the mouse trachea involving low-affinity choline transporters.
ESTHER : Nassenstein_2015_Int.Immunopharmacol_29(1)_173
PubMedSearch : Nassenstein_2015_Int.Immunopharmacol_29(1)_173
PubMedID: 26278668

Title : Dimeric bis (heptyl)-Cognitin Blocks Alzheimer's beta-Amyloid Neurotoxicity Via the Inhibition of Abeta Fibrils Formation and Disaggregation of Preformed Fibrils - Hu_2015_CNS.Neurosci.Ther_21_953
Author(s) : Hu SQ , Wang R , Cui W , Mak SH , Li G , Hu YJ , Lee MY , Pang YP , Han YF
Ref : CNS Neurosci Ther , 21 :953 , 2015
Abstract : AIMS: Fibrillar aggregates of beta-amyloid protein (Abeta) are the main constituent of senile plaques and considered to be one of the causative events in the pathogenesis of Alzheimer's disease (AD). Compounds that could inhibit Abeta fibrils formation, disaggregate preformed Abeta fibrils as well as reduce their associated neurotoxicity might have therapeutic values for treating AD. In this study, the inhibitory effects of bis (heptyl)-cognitin (B7C), a multifunctional dimer derived from tacrine, on aggregation and neurotoxicity of Abeta1-40 were evaluated both in vitro and in vivo.
METHODS: Thioflavin T fluorescence assay was carried out to evaluate Abeta aggregation, MTT and Hoechst-staining assays were performed to investigate Abeta-associated neurotoxicity. Fluorescent probe DCFH-DA was used to estimate the accumulation of intracellular reactive oxygen stress (ROS). Morris water maze was applied to determine learning and memory deficits induced by intracerebroventricular infusion of Abeta in rats.
RESULTS: B7C (0.1-10 muM), but not tacrine, effectively inhibited Abeta fibrils formation and disaggregated preformed Abeta fibrils following co-incubation of B7C and Abeta monomers or preformed fibrils, respectively. In addition, B7C markedly reduced Abeta fibrils-associated neurotoxicity in SH-SY5Y cell line, as evidenced by the increase in cell survival, the decrease in Hoechst-stained nuclei and in intracellular ROS. Most encouragingly, B7C (0.1 and 0.2 mg/kg), 10 times more potently than tacrine (1 and 2 mg/kg), inhibited memory impairments after intracerebroventricular infusion of Abeta in rats, as evidenced by the decrease in escape latency and the increase in the spatial bias in Morris water maze test along with upregulation of choline acetyltransferase activity and downregulation of acetylcholinesterase activity. CONCLUSION: These findings provide not only novel molecular insight into the potential application of B7C in treating AD, but also an effective approach for screening anti-AD agents.
ESTHER : Hu_2015_CNS.Neurosci.Ther_21_953
PubMedSearch : Hu_2015_CNS.Neurosci.Ther_21_953
PubMedID: 26507365

Title : Genome-wide transcriptomic analysis of a superior biomass-degrading strain of A. fumigatus revealed active lignocellulose-degrading genes - Miao_2015_BMC.Genomics_16_459
Author(s) : Miao Y , Liu D , Li G , Li P , Xu Y , Shen Q , Zhang R
Ref : BMC Genomics , 16 :459 , 2015
Abstract : BACKGROUND: Various saprotrophic microorganisms, especially filamentous fungi, can efficiently degrade lignocellulose that is one of the most abundant natural materials on earth. It consists of complex carbohydrates and aromatic polymers found in the plant cell wall and thus in plant debris. Aspergillus fumigatus Z5 was isolated from compost heaps and showed highly efficient plant biomass-degradation capability. RESULTS: The 29-million base-pair genome of Z5 was sequenced and 9540 protein-coding genes were predicted and annotated. Genome analysis revealed an impressive array of genes encoding cellulases, hemicellulases and pectinases involved in lignocellulosic biomass degradation. Transcriptional responses of A. fumigatus Z5 induced by sucrose, oat spelt xylan, Avicel PH-101 and rice straw were compared. There were 444, 1711 and 1386 significantly differently expressed genes in xylan, cellulose and rice straw, respectively, when compared to sucrose as a control condition. CONCLUSIONS: Combined analysis of the genomic and transcriptomic data provides a comprehensive understanding of the responding mechanisms to the most abundant natural polysaccharides in A. fumigatus. This study provides a basis for further analysis of genes shown to be highly induced in the presence of polysaccharide substrates and also the information which could prove useful for biomass degradation and heterologous protein expression.
ESTHER : Miao_2015_BMC.Genomics_16_459
PubMedSearch : Miao_2015_BMC.Genomics_16_459
PubMedID: 26076650
Gene_locus related to this paper: neofi-a1d0b8

Title : Aestuariivita atlantica sp. nov., isolated from deep-sea sediment - Li_2015_Int.J.Syst.Evol.Microbiol_65_3281
Author(s) : Li G , Lai Q , Du Y , Liu X , Sun F , Shao Z
Ref : Int J Syst Evol Microbiol , 65 :3281 , 2015
Abstract : A novel strain, 22II-S11-z3T, was isolated from the deep-sea sediment of the Atlantic Ocean. The bacterium was aerobic, Gram-staining-negative, oxidase-positive and catalase-negative, oval- to rod-shaped, and non-motile. Growth was observed at salinities of 1-9 % NaCl and temperatures of 10-45 degrees C. The isolate could hydrolyse aesculin and Tweens 20, 40 and 80, but not gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S11-z3T belonged to the genus Aestuariivita, with highest sequence similarity to Aestuariivita boseongensis KCTC 42052T (97.5 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain 22II-S11-z3T and A. boseongensis KCTC 42052T were 71.5 % and 20.0 +/- 2.3 %, respectively. The G+C content of the chromosomal DNA was 65.5 mol%. The principal fatty acids (>5 %) were summed feature 8 (C18 : 1omega7c/omega6c) (35.2 %), C19 : 0 cyclo omega8c (20.9 %), C16 : 0 (11.8 %), 11-methyl C18 : 1omega7c (11.4 %) and C12 : 1 3-OH (9.4 %). The respiratory quinone was determined to be Q-10. Diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, nine unidentified phospholipids, one unidentified aminolipid and two unidentified lipids were present. The combined genotypic and phenotypic data show that strain 22II-S11-z3T represents a novel species of the genus Aestuariivita, for which the name Aestuariivita atlantica sp. nov. is proposed, with the type strain 22II-S11-z3T ( = KCTC 42276T = MCCC 1A09432T).
ESTHER : Li_2015_Int.J.Syst.Evol.Microbiol_65_3281
PubMedSearch : Li_2015_Int.J.Syst.Evol.Microbiol_65_3281
PubMedID: 26297013
Gene_locus related to this paper: 9rhob-a0a0l1ju54

Title : Pseudooceanicola atlanticus gen. nov. sp. nov., isolated from surface seawater of the Atlantic Ocean and reclassification of Oceanicola batsensis, Oceanicola marinus, Oceanicola nitratireducens, Oceanicola nanhaiensis, Oceanicola antarcticus and Oceanicola flagellatus, as Pseudooceanicola batsensis comb. nov., Pseudooceanicola marinus comb. nov., Pseudooceanicola nitratireducens comb. nov., Pseudooceanicola nanhaiensis comb. nov., Pseudooceanicola antarcticus comb. nov., and Pseudooceanicola flagellatus comb. nov - Lai_2015_Antonie.Van.Leeuwenhoek_107_1065
Author(s) : Lai Q , Li G , Liu X , Du Y , Sun F , Shao Z
Ref : Antonie Van Leeuwenhoek , 107 :1065 , 2015
Abstract : A taxonomic study was carried out on strain 22II-S11g(T), which was isolated from the surface seawater of the Atlantic Ocean. The bacterium was found to be Gram-negative, rod shaped without flagellum, oxidase positive and weakly catalase positive. Growth was observed at NaCl concentrations of 0.5-9 % and at temperatures of 10-41 degrees C. The isolate was incapable of gelatin hydrolysis and unable to reduce nitrate to nitrite, degrade aesculin and Tween 80. On the basis of 16S rRNA gene sequence similarity, strain 22II-S11g(T) was found to be most closely related to Oceanicola batsensis HTCC2597(T) (97.26 %), followed by Oceanicola nitratireducens JLT1210(T) (96.39 %), whilst other species of genus Oceanicola shared 94.00-96.34 % sequence similarity. However, it showed low similarity to Oceanicola granulosus HTCC2516(T) (94.79 %), the type species of the genus Oceanicola. Phylogenetic analysis showed that strain 22II-S11g(T) formed a clade with six species currently classified in the genus Oceanicola, but strain O. granulosus HTCC2516(T) and strain O. litoreus M-M22(T) clustered with two other genera respectively. The ANI values between strain 22II-S11g(T) and two type strains (O. batsensis HTCC2597(T) and O. granulosus HTCC2516(T)) are 91.86 and 91.81 % respectively. The digital DNA-DNA hybridization estimate values between strain 22II-S11g(T) and two type strains (O. batsensis HTCC2597(T) and O. granulosus HTCC2516(T)) are 23.4 +/- 2.4 and 20.0 +/- 2.3 %, respectively. The principal fatty acids were identified as summed feature 8 (C18:1 omega7c/omega6c), C16:0, C18:1 omega7c11-methyl and C12:0 3OH. The G+C content determined from the draft genome sequence is 64.1 mol%. The respiratory quinone was determined to be Q-10 (100 %). Phosphatidylethanolamine, phosphatidylglycerol, an aminolipid, phosphatidylcholine, a phospholipid and three lipids were identified in the polar lipids. The combined genotypic and phenotypic data also show that strain 22II-S11g(T) should not be assigned to the genus Oceanicola; consequently strain 22II-S11g(T) is concluded to represent a novel species of a novel genus in the family Rhodobacteraceae, for which the name Pseudooceanicola atlanticus gen. nov., sp. nov. is proposed (type strain 22II-S11g(T) = KCTC 42004(T) = LMG 27424(T) = MCCC 1A09160(T)). Six misclassified species should be transferred to the novel genus Pseudooceanicola as follows: O. batsensis should be transferred to the genus Pseudooceanicola as Pseudooceanicola batsensis comb. nov. (type strain HTCC2597(T) = ATCC BAA-863(T) = DSM 15984(T) = KCTC 12145(T)); Oceanicola marinus should be transferred to the genus Pseudooceanicola as Pseudooceanicola marinus comb. nov. (type strain AZO-C(T) = LMG 23705(T) = BCRC 17591(T)); O. nitratireducens should be transferred to the genus Pseudooceanicola as Pseudooceanicola nitratireducens comb. nov. (type strain JLT1210(T) = LMG 24663(T) = CGMCC 1.7292(T)); Oceanicola nanhaiensis should be transferred to the genus Pseudooceanicola as Pseudooceanicola nanhaiensis comb. nov. (type strain SS011B1-20(T) = LMG 23508(T) = CGMCC 1.6293(T)); Oceanicola antarcticus should be transferred to the genus Pseudooceanicola as Pseudooceanicola antarcticus comb. nov. (type strain Ar-45(T) = CGMCC 1.12662(T) = LMG 27868(T)); and Oceanicola flagellatus should be transferred to the genus Pseudooceanicola as Pseudooceanicola flagellatus comb. nov. (type strain DY470(T) = CGMCC 1.12664(T) = LMG 27871(T)).
ESTHER : Lai_2015_Antonie.Van.Leeuwenhoek_107_1065
PubMedSearch : Lai_2015_Antonie.Van.Leeuwenhoek_107_1065
PubMedID: 25663028
Gene_locus related to this paper: 9rhob-a0a0a0eep0

Title : Preventive Effect of Lactobacillus fermentum Zhao on Activated Carbon-Induced Constipation in Mice - Zhao_2015_J.Nutr.Sci.Vitaminol.(Tokyo)_61_131
Author(s) : Zhao X , Qian Y , Suo H , Du M , Li G , Liu Z , Li J
Ref : J Nutr Sci Vitaminol (Tokyo) , 61 :131 , 2015
Abstract : The aim of this study was to investigate the effects of Lactobacillus fermentum Zhao (LF-Zhao) on activated carbon-induced constipation in ICR mice. ICR mice were administered lactic acid bacteria by gavage for 9 d. Body weight, diet intake, drinking amount, stool status, gastrointestinal transit distance and stool time, in addition to motilin (MTL), gastrin (Gas), endothelin (ET), somatostatin (SS), acetylcholinesterase (AChE), substance P (SP) and vasoactive intestinal peptide (VIP) levels in serum were monitored to evaluate the preventive effects of LF-Zhao on constipation. Bisacodyl, a laxative drug, was used as a positive control. Times to the first black stool for normal (untreated), control (no lactic acid bacteria treatment but activated carbon treated), bisacodyl-treated and L. delbrueckii subsp. bulgaricus (LB), LF-Zhao (L) (low concentration of 1x10(8) CFU/mL)- and LF-Zhao (H) (high concentration of 1x10(9) CFU/mL)-treated mice induced by activated carbon were 90, 218, 117, 180, 169 and 156 min, respectively. Following the consumption of LB, LF-Zhao (L) and LF-Zhao (H) or the oral administration of bisacodyl, the gastrointestinal transit distances were reduced by 55.2%, 61.3%, 70.6% and 94.6%, respectively. The serum levels of MTL, Gas, ET, AChE, SP and VIP were significantly increased and the serum levels of SS were reduced in the mice treated with LF-Zhao compared with those in the control mice (p<0.05). These results demonstrated that lactic acid bacteria demonstrate preventive effects on mouse constipation and that LF-Zhao alleviated constipation symptoms better than LB.
ESTHER : Zhao_2015_J.Nutr.Sci.Vitaminol.(Tokyo)_61_131
PubMedSearch : Zhao_2015_J.Nutr.Sci.Vitaminol.(Tokyo)_61_131
PubMedID: 26052143

Title : Tamlana nanhaiensis sp. nov., isolated from surface seawater collected from the South China Sea - Liu_2015_Antonie.Van.Leeuwenhoek_107_1189
Author(s) : Liu X , Lai Q , Du Y , Li G , Sun F , Shao Z
Ref : Antonie Van Leeuwenhoek , 107 :1189 , 2015
Abstract : A polyphasic taxonomic study was performed on a strain, designated FHC16(T), which was isolated from surface seawater collected from the South China Sea. Cells of strain FHC16(T) are Gram stain-negative, oxidase- and catalase-positive and non-motile rods. Growth was observed at 15-37 degreesC (optimum, 25-30 degreesC), at pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-5 % (w/v) NaCl (optimum, 3%). 16S rRNA gene sequence analysis showed that strain FHC16(T) is most closely related to Tamlana sedimentorum JCM 19808(T) (98.2% sequence similarity). The ANI value between strain FHC16(T) and T. sedimentorum JCM 19808(T) was found to be 81.82-81.81%. The DNA-DNA hybridization estimated value between strain FHC16(T) and T. sedimentorum JCM 19808(T) was determined to be 25.8 +/- 2.41%. The principal fatty acids (>5% of the total) were found to be iso-C(15:0), iso G-C(15:1), iso-C(17:0) 3-OH, iso-C(15:0) 3-OH and summed feature 3 (comprising C(16:1)omega7c/C(16:1)omega6c). The strain was found to have MK-6 as the major respiratory menaquinone, which is consistent with the other three recognized Tamlana species, T. sedimentorum, Tamlana crocina and Tamlana agarivorans. The polar lipids were found to comprise phosphatidylethanolamine, one unidentified aminophospholipid, two unidentified aminolipids and seven unidentified lipids. The G+C content of the chromosomal DNA was determined to be 34.2 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain FHC16(T) is considered to represent a novel species of the genus Tamlana, for which the name Tamlana nanhaiensis sp. nov. is proposed. The type strain is FHC16(T) (.LMG 27420(T) = CGMCC 1.12469(T) = MCCC 1A06648(T)).
ESTHER : Liu_2015_Antonie.Van.Leeuwenhoek_107_1189
PubMedSearch : Liu_2015_Antonie.Van.Leeuwenhoek_107_1189
PubMedID: 25735434
Gene_locus related to this paper: 9flao-a0a0d7w993

Title : Lignin binding to pancreatic lipase and its influence on enzymatic activity - Zhang_2014_Food.Chem_149_99
Author(s) : Zhang J , Xiao L , Yang Y , Wang Z , Li G
Ref : Food Chem , 149 :99 , 2014
Abstract : In this paper, we find that the effect of lignin on pancreatic lipase (PL) is dependent on reaction medium and substrate used. Experimental results reveal that lignin can gradually bind to PL to form a PL-lignin complex, resulting in an increased activity of the enzyme. The binding process is spontaneous and the PL-lignin complex formation is an endothermic reaction induced by hydrophobic and electrostatic interaction. There is a non-radiation energy transfer from PL to lignin during the binding process, and the binding of lignin to PL conforms to a secondary exponential decay function. Moreover, the alpha-helix content of the enzyme will be changed and the rigidity of its side chain will be enhanced due to the formation of lignin-PL complex. This study has not only provided the activation effect of lignin on PL, but also given an insight into the interaction between lignin and the enzyme, which would benefit the application of lignin in the pharmacy and food industry, as well as other fields.
ESTHER : Zhang_2014_Food.Chem_149_99
PubMedSearch : Zhang_2014_Food.Chem_149_99
PubMedID: 24295682

Title : Therapeutic Effect of Activated Carbon-Induced Constipation Mice with Lactobacillus fermentum Suo on Treatment - Suo_2014_Int.J.Mol.Sci_15_21875
Author(s) : Suo H , Zhao X , Qian Y , Li G , Liu Z , Xie J , Li J
Ref : Int J Mol Sci , 15 :21875 , 2014
Abstract : The aim of this study was to investigate the effects of Lactobacillus fermentum Suo (LF-Suo) on activated carbon-induced constipation in ICR (Institute of Cancer Research) mice. ICR mice were orally administered with lactic acid bacteria for 9 days. Body weight, diet intake, drinking amount, defecation status, gastrointestinal transit and defecation time, and the serum levels of MTL (motilin), Gas (gastrin), ET (endothelin), SS (somatostatin), AChE (acetylcholinesterase), SP (substance P), VIP (vasoactive intestinal peptide) were used to evaluate the preventive effects of LF-Suo on constipation. Bisacodyl, a laxative drug, was used as a positive control. The normal, control, 100 mg/kg bisacodyl treatment, LB (Lactobacillus bulgaricus)-, LF-Suo (L)- and LF-Suo (H)-treated mice showed the time to the first black stool defecation at 90, 218, 117, 180, 155 and 137 min, respectively. By the oral administration of LB-, LF-Suo (L), LF-Suo (H) or bisacodyl (100 mg/kg), the gastrointestinal transit was reduced to 55.2%, 72.3%, 85.5% and 94.6%, respectively, of the transit in normal mice, respectively. In contrast to the control mice, the serum levels of MTL, Gas, ET, AChE, SP and VIP were significantly increased and the serum levels of SS were reduced in the mice treated with LF-Suo (p < 0.05). By the RT-PCR (reverse transcription-polymerase chain reaction) and western blot assays, LF-Suo increased the c-Kit, SCF (stem cell factor), GDNF (glial cell line-derived neurotrophic factor) and decreased TRPV1 (transient receptor potential vanilloid 1), NOS (nitric oxide synthase) expressions of small intestine tissue in mice. These results demonstrate that lactic acid bacteria has preventive effects on mouse constipation and LF-Suo demonstrated the best functional activity.
ESTHER : Suo_2014_Int.J.Mol.Sci_15_21875
PubMedSearch : Suo_2014_Int.J.Mol.Sci_15_21875
PubMedID: 25464378

Title : Hyphomonas beringensis sp. nov. and Hyphomonas chukchiensis sp. nov., isolated from surface seawater of the Bering Sea and Chukchi Sea - Li_2014_Antonie.Van.Leeuwenhoek_106_657
Author(s) : Li C , Lai Q , Li G , Dong C , Wang J , Liao Y , Shao Z
Ref : Antonie Van Leeuwenhoek , 106 :657 , 2014
Abstract : Two Gram-negative, non-spore-forming, oval to pear shaped motile strains, designated 25B14_1(T) and BH-BN04-4(T), isolated from surface seawater from the Bering Sea and Chukchi Sea, respectively, were subjected to polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strains 25B14_1(T) and BH-BN04-4(T) clustered together with Hyphomonas atlanticus 22II1-22F38(T) and Hyphomonas oceanitis DSM 5155(T), respectively, within genus Hyphomonas. Based on whole genome sequence analysis, the calculated DDH and ANIm values between strain 25B14_1(T) and BH-BN04-4(T) are 18.8 and 83.19% respectively. The calculated DDH values of strain 25B14_1(T) and BH-BN04-4(T) with seven type strains ranged from 18.2 to 19.9% and from 18.4 to 40.4%, respectively. The ANIm values of strain 25B14_1(T) and BH-BN04-4(T) with seven type strains ranged from 83.00 to 84.67% and from 83.14 to 90.58%, respectively. Both isolates were found to contain Q-11 as the predominant respiratory quinone. The major fatty acids of strain 25B14_1(T) were identified as C(16:0), C(17:0), C(18:1)omega7c-methyl and Summed Feature 8 (C(18:1)omega6c/omega7c as defined by MIDI), while in the case of strain BH-BN04-4(T) they were identified as C(16:0), C(18:1)omega7c-methyl and Summed Feature 8 (C(18:1)omega6c/omega7c). The G+C contents of 25B14_1(T) and BH-BN04-4(T) were determined to be 58.4 and 61.0 mol%, respectively. The combined phenotypic and genotypic data show that the two isolates each represent novel species of the genus Hyphomonas, for which the names Hyphomonas beringensis sp. nov. and Hyphomonas chukchiensis sp. nov. are proposed, with the type strain 25B14_1(T) (=MCCC 1A07321(T) = LMG 27914(T)) and BH-BN04-4(T) (=MCCC 1A07481(T) = LMG 27915(T)), respectively.
ESTHER : Li_2014_Antonie.Van.Leeuwenhoek_106_657
PubMedSearch : Li_2014_Antonie.Van.Leeuwenhoek_106_657
PubMedID: 25070064
Gene_locus related to this paper: 9rhob-a0a059fvx5 , 9rhob-a0a062ve25 , 9rhob-a0a059e3y9 , 9rhob-a0a062tww8 , 9rhob-a0a062u829 , 9rhob-a0a059e1b7 , 9rhob-a0a059g4l9 , 9rhob-a0a059f7b2 , 9rhob-a0a069e8d0 , 9rhob-a0a059g313 , 9rhob-a0a059g3u2 , 9rhob-a0a059fuw7 , 9rhob-a0a062ui03 , 9rhob-a0a059fgt4 , 9rhob-a0a059dz53 , 9rhob-a0a069e7i5 , 9rhob-a0a059fgq3 , 9rhob-a0a069e822 , 9rhob-a0a062vhu5 , 9rhob-a0a062uj80 , 9rhob-a0a062udx1 , 9rhob-a0a059eck3 , 9rhob-a0a062u513 , 9rhob-a0a062vh83 , 9rhob-a0a062u677 , 9rhob-a0a069e9p2 , 9rhob-a0a062ui16 , 9rhob-a0a059g4x1 , 9rhob-a0a062u908 , 9rhob-a0a062uqj8 , 9rhob-a0a062u917 , 9rhob-a0a059fty9 , 9rhob-a0a062uq79 , 9rhob-a0a059g214 , 9rhob-a0a062uez6 , 9rhob-a0a062v9r9 , 9rhob-a0a059g243 , 9rhob-a0a059fbn4 , 9rhob-a0a069e4q5 , 9rhob-a0a069e8h3 , 9rhob-a0a059dyu6 , 9rhob-a0a059fva5 , 9rhob-a0a059g4g8 , 9rhob-a0a059f9m8 , 9rhob-a0a062vgs9

Title : Highly sensitive assay for acetylcholinesterase activity and inhibition based on a specifically reactive photonic nanostructure - Tian_2014_ACS.Appl.Mater.Interfaces_6_15456
Author(s) : Tian T , Li X , Cui J , Li J , Lan Y , Wang C , Zhang M , Wang H , Li G
Ref : ACS Appl Mater Interfaces , 6 :15456 , 2014
Abstract : Assays for acetylcholinesterase (AChE) with high sensitivity and high selectivity as well as facile manipulation have been urgently required in various fields. In this work, a reaction-based photonic strategy was developed for the efficient assay of AChE activity and inhibition based on the synergetic combination of the specific thiol-maleimide addition reaction with photonic porous structure. It was found that various applications including detection of AChE activity, measurement of the related enzymatic kinetics, and screening of inhibitors could be efficiently implemented using such strategy. Remarkably, the unique photonic nanostructure endows the constructed sensing platform with high sensitivity with a limit of detection (LOD) of 5 mU/mL for AChE activity, high selectivity, and self-reporting signaling. Moreover, the label-free solid film-based sensing approach described here has advantages of facile manipulation and bare-eye readout, compared with conventional liquid-phase methods, exhibiting promising potential in practical application for the AChE assay.
ESTHER : Tian_2014_ACS.Appl.Mater.Interfaces_6_15456
PubMedSearch : Tian_2014_ACS.Appl.Mater.Interfaces_6_15456
PubMedID: 25130420

Title : Roseivivax atlanticus sp. nov., isolated from surface seawater of the Atlantic Ocean - Li_2014_Antonie.Van.Leeuwenhoek_105_863
Author(s) : Li G , Lai Q , Liu X , Sun F , Shao Z
Ref : Antonie Van Leeuwenhoek , 105 :863 , 2014
Abstract : A taxonomic study was carried out on strain 22II-S10s(T), which was isolated from the surface seawater of the Atlantic Ocean. The bacterium was found to be Gram-negative, oxidase and catalase positive, rod shaped and motile by subpolar flagella. The isolate was capable of gelatine hydrolysis but unable to reduce nitrate to nitrite or degrade Tween 80 or aesculin. Growth was observed at salinities of 0.5-18 % (optimum, 2-12 %), at pH of 3-10 (optimum, 7) and at temperatures of 10-41 degrees C (optimum 28 degrees C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S10s(T) belongs to the genus Roseivivax, with highest sequence similarity to Roseivivax halodurans JCM 10272(T) (97.2 %), followed by Roseivivax isoporae LMG 25204(T) (97.0 %); other species of genus Roseivivax shared 95.2-96.7 % sequence similarity. The DNA-DNA hybridization estimate values between strain 22II-S10s(T) and the two type strains (R. halodurans JCM 10272(T) and R. isoporae LMG 25204(T)) were 22.00 and 21.40 %. The principal fatty acids were identified as Summed Feature 8 (C18:1 omega7c/omega6c) (67.4 %), C18:0 (7.2 %), C19:0 cyclo omega8c (7.1 %), C18:1 omega7c 11-methyl (6.8 %) and C16:0 (5.9 %). The respiratory quinone was determined to be Q-10 (100 %). Phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an aminolipid, a glycolipid and three phospholipids were present. The G+C content of the chromosomal DNA was determined to be 67.5 mol%. The combined genotypic and phenotypic data show that strain 22II-S10s(T) represents a novel species within the genus Roseivivax, for which the name Roseivivax atlanticus sp. nov. is proposed, with the type strain 22II-S10s(T) (= MCCC 1A09150(T) = LMG 27156(T)).
ESTHER : Li_2014_Antonie.Van.Leeuwenhoek_105_863
PubMedSearch : Li_2014_Antonie.Van.Leeuwenhoek_105_863
PubMedID: 24567080
Gene_locus related to this paper: 9rhob-w4hpi1 , 9rhob-w4hj54 , 9rhob-w4hpb0

Title : Pathophysiological amyloid concentrations induce sustained upregulation of readthrough acetylcholinesterase mediating anti-apoptotic effects - Li_2013_Neurosci_240_349
Author(s) : Li G , Klein J , Zimmermann M
Ref : Neuroscience , 240 :349 , 2013
Abstract : Cholinergically differentiated SH-SY5Y neuroblastoma cells were treated with a pathophysiologically relevant, low (300 nM), and a high (3 muM) dose of amyloid beta 1-42 (Abeta) or 42-1 (revAbeta). At early (1 and 4h) and late (24h) time points, the pro- and anti-apoptotic factors--caspase-3 and p53, and B-cell lymphoma 2 protein (Bcl-2), respectively--were assessed together with lactate dehydrogenase (LDH) release as measure of cell viability and ATP levels as marker of mitochondrial activity. The low peptide dose significantly increased Bcl-2 and, time-delayed, caspase-3 and ATP levels, but barely impacted on LDH release, while the high concentration remarkably depressed Bcl-2 levels, depleted ATP and led to increased LDH release. We also monitored acetylcholinesterase (AChE) enzymatic activity and splice variant levels (tailed and readthrough AChE; AChE-T and AChE-R), and assessed choline acetyltransferase (ChAT) and high-affinity choline uptake (HACU). The low Abeta concentration drastically upregulated AChE-R and increased both ChAT and HACU, while the high dose caused cholinergic toxicity. We believe this study offers the first insight into the highly concentration-dependent effects of Abeta on cholinergic dynamics. In particular, it highlights the rescuing role of AChE-R as being, together with mitochondrial activity, involved in cholinergic adaptation to low doses of Abeta.
ESTHER : Li_2013_Neurosci_240_349
PubMedSearch : Li_2013_Neurosci_240_349
PubMedID: 23485809

Title : Complete Genome Sequence of Vibrio anguillarum M3, a Serotype O1 Strain Isolated from Japanese Flounder in China - Li_2013_Genome.Announc_1_e00769
Author(s) : Li G , Mo Z , Li J , Xiao P , Hao B
Ref : Genome Announc , 1 :e00769 , 2013
Abstract : Vibrio anguillarum is an important bacterial pathogen that causes vibriosis in marine fish. We present the complete genome sequence of V. anguillarum M3, a serotype O1 clinical strain isolated from Japanese flounder (Paralichthys olivaceus) in Shandong, China.
ESTHER : Li_2013_Genome.Announc_1_e00769
PubMedSearch : Li_2013_Genome.Announc_1_e00769
PubMedID: 24072867
Gene_locus related to this paper: viba7-f7ylq1 , viba7-f7ypr9

Title : Draft Genome Sequence of a Benzothiophene-Desulfurizing Bacterium, Gordona terrae Strain C-6 - Wang_2013_Genome.Announc_1_e00381
Author(s) : Wang W , Ma T , Ren Y , Li G
Ref : Genome Announc , 1 : , 2013
Abstract : Gordona terrae strain C-6 was isolated from oil-contaminated soil and is capable of desulfurizing benzothiophene (BT). Here we report the draft genome sequence of G. terrae strain C-6, which may help to reveal the genetic basis of the BT biodesulfurization pathway.
ESTHER : Wang_2013_Genome.Announc_1_e00381
PubMedSearch : Wang_2013_Genome.Announc_1_e00381
PubMedID: 23788548
Gene_locus related to this paper: 9acto-h5ue78 , 9acto-h5ujj3 , 9acto-j9rte6 , 9acto-j9rnp4 , 9acto-h5ui26 , 9actn-r7y6f1 , 9actn-r7ybz2

Title : Draft Genome Sequence of Ralstonia solanacearum Race 4 Biovar 4 Strain SD54 - Shan_2013_Genome.Announc_1_e00890
Author(s) : Shan W , Yang X , Ma W , Yang Y , Guo X , Guo J , Zheng H , Li G , Xie B
Ref : Genome Announc , 1 : , 2013
Abstract : Ralstonia solanacearum is an important etiological agent that can cause serious bacterial wilt in a very wide range of potential host plants, including ginger. Here, we report the complete genome sequence of R. solanacearum SD54, a race 4 biovar 4 (R4B4) strain from a diseased ginger plant in China.
ESTHER : Shan_2013_Genome.Announc_1_e00890
PubMedSearch : Shan_2013_Genome.Announc_1_e00890
PubMedID: 24356823
Gene_locus related to this paper: ralso-PCAD

Title : Microsomal epoxide hydrolase (EPHX1) polymorphisms are associated with aberrant promoter methylation of ERCC3 and hematotoxicity in benzene-exposed workers - Xing_2013_Environ.Mol.Mutagen_54_397
Author(s) : Xing C , Chen Q , Li G , Zhang L , Zheng M , Zou Z , Hou L , Wang QF , Liu X , Guo X
Ref : Environmental & Molecular Mutagenesis , 54 :397 , 2013
Abstract : Benzene is an important industrial chemical and widespread environmental pollutant known to induce leukemia and other blood disorders. To be carcinogenic, benzene must be metabolized to produce toxic metabolites. To investigate whether single nucleotide polymorphisms (SNPs) in the metabolic enzyme genes are associated with benzene-induced alterations in DNA methylation and hematotoxicity, we genotyped four commonly studied SNPs in three metabolic enzymes genes CYP1A1, EPHX1 and NQO1; and analyzed promoter DNA methylation status in 11 genes which have been reported to be associated with benzene-induced hematotoxicity (BLM, CYP1A1, EPHX1, ERCC3, NQO1, NUDT1, p15, p16, RAD51, TP53 and WRAP53) in 77 benzene-exposed workers and 25 unexposed controls in China. ERCC3, a DNA repair gene, showed a small but statistically significant increase of promoter DNA methylation in the exposed group compared with the unexposed group (mean +/- SD: 4.73 +/- 3.46% vs. 3.63 +/- 1.96%, P = 0.048). We also observed that an increased number of C allele for rs1051740 in EPHX1 was associated with decreased ERCC3 methylation levels in benzene-exposed workers (P(trend) = 0.001), but not in unexposed controls (P(trend) = 0.379). Interestingly, another EPHX1 SNP (rs2234922) was associated with lower white blood cell (WBC) counts (P(trend) = 0.044) in benzene-exposed workers. These associations remained the same when ERCC3 promoter methylation and WBCs were dichotomized according to the 90th percentile (>/=6%) of methylation levels in controls and a leucopenia cutoff (<4 x 10(9) /L), respectively. Our findings suggest that benzene exposure may be associated with hypermethylation in ERCC3, and that genetic variants in EPHX1 may play an important role in epigenetic changes and hematotoxicity among benzene-exposed workers.
ESTHER : Xing_2013_Environ.Mol.Mutagen_54_397
PubMedSearch : Xing_2013_Environ.Mol.Mutagen_54_397
PubMedID: 23797950

Title : Investigation Binding Patterns of Human Carboxylesterase I (hCES I) with Broad Substrates by MD Simulations - Chu_2013_Curr.Top.Med.Chem_13_1222
Author(s) : Chu H , Min H , Zhang M , Shen H , Li G
Ref : Curr Top Med Chem , 13 :1222 , 2013
Abstract : Human carboxylesterase I (hCES 1) plays an important role in the metabolism and activation of prodrugs, such as, the hydrolysis of a variety of drugs of prodrugs featuring an ester, amide or carbamate function. The bindings of the substrates of different lengths and cocaine to hCES1 at two different binding sites, catalytic site and Z-site, were studies through MD simulations. For each case, the correlation analysis has been performed to explore the binding patterns of a broad range of substrates binding to the hCES1.
ESTHER : Chu_2013_Curr.Top.Med.Chem_13_1222
PubMedSearch : Chu_2013_Curr.Top.Med.Chem_13_1222
PubMedID: 23647544

Title : Complete genome sequence of the avian pathogenic Escherichia coli strain APEC O78 - Mangiamele_2013_Genome.Announc_1_e0002613
Author(s) : Mangiamele P , Nicholson B , Wannemuehler Y , Seemann T , Logue CM , Li G , Tivendale KA , Nolan LK
Ref : Genome Announc , 1 :e0002613 , 2013
Abstract : Colibacillosis, caused by avian pathogenic Escherichia coli (APEC), is a significant disease, causing extensive animal and financial losses globally. Because of the significance of this disease, more knowledge is needed regarding APEC's mechanisms of virulence. Here, we present the fully closed genome sequence of a typical avian pathogenic E. coli strain belonging to the serogroup O78.
ESTHER : Mangiamele_2013_Genome.Announc_1_e0002613
PubMedSearch : Mangiamele_2013_Genome.Announc_1_e0002613
PubMedID: 23516182
Gene_locus related to this paper: ecoli-ycfp , ecoli-yqia , ecoli-YfhR

Title : Genome sequence of Nitratireductor pacificus type strain pht-3B - Lai_2012_J.Bacteriol_194_6958
Author(s) : Lai Q , Li G , Shao Z
Ref : Journal of Bacteriology , 194 :6958 , 2012
Abstract : Nitratireductor pacificus strain pht-3B(T) was isolated from a pyrene-degrading consortium enriched from the deep sea sediment of the Pacific Ocean. Here, we present the draft genome of strain pht-3B(T), which contains 4,466,205 bp with a G+C content of 65.51% and contains 4,197 protein-coding genes and 46 tRNA genes.
ESTHER : Lai_2012_J.Bacteriol_194_6958
PubMedSearch : Lai_2012_J.Bacteriol_194_6958
PubMedID: 23209217
Gene_locus related to this paper: 9rhiz-k2lkd0 , 9rhiz-k2mk55

Title : Glycosyl-phosphatidylinositol (GPI)-anchored membrane association of the porcine reproductive and respiratory syndrome virus GP4 glycoprotein and its co-localization with CD163 in lipid rafts - Du_2012_Virology_424_18
Author(s) : Du Y , Pattnaik AK , Song C , Yoo D , Li G
Ref : Virology , 424 :18 , 2012
Abstract : The porcine reproductive and respiratory syndrome virus (PRRSV) glycoprotein 4 (GP4) resembles a typical type I membrane protein in its structure but lacks a hydrophilic tail at the C-terminus, suggesting that GP4 may be a lipid-anchored membrane protein. Using the human decay-accelerating factor (DAF; CD55), a known glycosyl-phosphatidylinositol (GPI) lipid-anchored protein, chimeric constructs were made to substitute the GPI-anchor domain of DAF with the putative lipid-anchor domain of GP4, and their membrane association and lipase cleavage were determined in cells. The DAF-GP4 fusion protein was transported to the plasma membrane and was cleaved by phosphatidylinositol-specific phospholipase C (PI-PLC), indicating that the C-terminal domain of GP4 functions as a GPI anchor. Mutational studies for residues adjacent to the GPI modification site and characterization of respective mutant viruses generated from infectious cDNA clones show that the ability of GP4 for membrane association corresponded to virus viability and growth characteristics. The residues T158 (omega-2, where omega is the GPI moiety at E160), P159 (omega-1), and M162 (omega+2) of GP4 were determined to be important for virus replication, with M162 being of particular importance for virus infectivity. The complete removal of the peptide-anchor domain in GP4 resulted in a complete loss of virus infectivity. The depletion of cholesterol from the plasma membrane of cells reduced the virus production, suggesting a role of lipid rafts in PRRSV infection. Remarkably, GP4 was found to co-localize with CD163 in the lipid rafts on the plasma membrane. Since CD163 has been reported as a cellular receptor for PRRSV and GP4 has been shown to interact with this receptor, our data implicates an important role of lipid rafts during entry of the virus.
ESTHER : Du_2012_Virology_424_18
PubMedSearch : Du_2012_Virology_424_18
PubMedID: 22222209

Title : Genome sequence of Nitratireductor indicus type strain C115 - Lai_2012_J.Bacteriol_194_6990
Author(s) : Lai Q , Li G , Yu Z , Shao Z
Ref : Journal of Bacteriology , 194 :6990 , 2012
Abstract : Nitratireductor indicus strain C115(T) was isolated from a crude-oil-degrading consortium enriched from deep seawater of the Indian Ocean. Here, we present the draft genome of strain C115(T), which contains 4,992,479 bp with a G+C content of 60.8% and contains 4,825 protein-coding genes and 45 tRNA genes.
ESTHER : Lai_2012_J.Bacteriol_194_6990
PubMedSearch : Lai_2012_J.Bacteriol_194_6990
PubMedID: 23209238
Gene_locus related to this paper: 9rhiz-k2nwj6 , 9rhiz-k2pjb4 , 9rhiz-k2ns58 , 9rhiz-k2piz2

Title : Biocatalytic Synthesis of Poly(delta-Valerolactone) Using a Thermophilic Esterase from Archaeoglobus fulgidus as Catalyst - Cao_2012_Int.J.Mol.Sci_13_12232
Author(s) : Cao H , Han H , Li G , Yang J , Zhang L , Yang Y , Fang X , Li Q
Ref : Int J Mol Sci , 13 :12232 , 2012
Abstract : The ring-opening polymerization of delta-valerolactone catalyzed by a thermophilic esterase from the archaeon Archaeoglobus fulgidus was successfully conducted in organic solvents. The effects of enzyme concentration, temperature, reaction time and reaction medium on monomer conversion and product molecular weight were systematically evaluated. Through the optimization of reaction conditions, poly(delta-valerolactone) was produced in 97% monomer conversion, with a number-average molecular weight of 2225 g/mol, in toluene at 70 degrees C for 72 h. This paper has produced a new biocatalyst for the synthesis of poly(delta-valerolactone), and also deeper insight has been gained into the mechanism of thermophilic esterase-catalyzed ring-opening polymerization.
ESTHER : Cao_2012_Int.J.Mol.Sci_13_12232
PubMedSearch : Cao_2012_Int.J.Mol.Sci_13_12232
PubMedID: 23202895

Title : Staurosporine-induced apoptosis presents with unexpected cholinergic effects in a differentiated neuroblastoma cell line - Li_2012_Neurochem.Int_61_1011
Author(s) : Li G , Gleinich A , Lau H , Zimmermann M
Ref : Neurochem Int , 61 :1011 , 2012
Abstract : Apoptosis of cholinergic neurons is one of the core hallmarks of Alzheimer's disease. SH-SY5Y neuroblastoma cells differentiated to the cholinergic phenotype were exposed to 100nM staurosporine. Over a treatment period of 24h, the pro- and anti-apoptotic factors, caspase-3 and Bcl-2, as well as LDH release as a measure of cell viability, were assessed in conjunction with the number of apoptotic cells by means of fluorescence-activated cell sorting. Caspase-3 activity and LDH release increased by 30% and 20% over controls, respectively, while Bcl-2 levels rose by 200% over controls. Furthermore, staurosporine treatment resulted in decreased acetylcholinesterase (AChE) enzymatic activity and decreased protein levels of the AChE splice variant tailed AChE (AChE-T). Only a slight increase in levels of readthrough AChE (AChE-R) was observed. Likewise, staurosporine reduced levels and activity of the cholinergic players choline acetyltransferase and high affinity choline uptake. The present study demonstrates that treatment with staurosporine leads to apoptotic events, which, however, are not reflected in the increased AChE activity and the alterations of AChE isoforms expression that are usually seen in apoptotic conditions. The effects of various additional phosphorylation inhibitors on AChE activity suggest that these unexpected cholinergic effects, firstly, are linked to the impact of staurosporine on phosphorylation and, secondly, reveal themselves in a first phase of cellular adaption that precedes neurotoxicity and subsequent cell death.
ESTHER : Li_2012_Neurochem.Int_61_1011
PubMedSearch : Li_2012_Neurochem.Int_61_1011
PubMedID: 22841891

Title : Complete genome sequence of Pusillimonas sp. T7-7, a cold-tolerant diesel oil-degrading bacterium isolated from the Bohai Sea in China - Cao_2011_J.Bacteriol_193_4021
Author(s) : Cao B , Ma T , Ren Y , Li G , Li P , Guo X , Ding P , Feng L
Ref : Journal of Bacteriology , 193 :4021 , 2011
Abstract : Pusillimonas sp. T7-7 is a diesel oil-degrading cold-tolerant bacterium isolated from the benthal mud of a petroleum-contaminated site in Bohai Sea, China. We present here the complete genome sequence of T7-7. Genome analysis revealed many features of typical marine bacteria, including the absence of intact sugar metabolic pathways, the presence of glyoxylate and gluconeogenesis pathways, and the abilities for nitrate assimilation and denitrification, as well as sulfate reduction and sulfite oxidation. The presence of novel genes for the degradation of diesel oils was suggested.
ESTHER : Cao_2011_J.Bacteriol_193_4021
PubMedSearch : Cao_2011_J.Bacteriol_193_4021
PubMedID: 21622753
Gene_locus related to this paper: pusst-f4grw7

Title : Accelerated evolution of CES7, a gene encoding a novel major urinary protein in the cat family - Li_2011_Mol.Biol.Evol_28_911
Author(s) : Li G , Janecka JE , Murphy WJ
Ref : Molecular Biology Evolution , 28 :911 , 2011
Abstract : Cauxin is a novel urinary protein recently identified in the domestic cat that regulates the excretion of felinine, a pheromone precursor involved in sociochemical communication and territorial marking of domestic and wild felids. Understanding the evolutionary history of cauxin may therefore illuminate molecular adaptations involved in the evolution of pheromone-based communication, recognition, and mate selection in wild animals. We sequenced the gene encoding cauxin, CES7, in 22 species representing all major felid lineages, and multiple outgroups and showed that it has undergone rapid evolutionary change preceding and during the diversification of the cat family. A comparison between feline cauxin and orthologous carboxylesterases from other mammalian lineages revealed evidence of strong positive Darwinian selection within and between several cat lineages, enriched at functionally important sites of the protein. The higher rate of radical amino acid replacements in small felids, coupled with the lack of felinine and extremely low levels of cauxin in the urine of the great cats (Panthera), correlates with functional divergence of this gene in Panthera, and its putative loss in the snow leopard. Expression studies found evidence for several alternatively spliced transcripts in testis and brain, suggesting additional roles in male reproductive fitness and behavior. Our work presents the first report of strong positive natural selection acting on a major urinary protein of nonrodent mammals, providing evidence for parallel selection pressure on the regulation of pheromones in different mammalian lineages, despite the use of different metabolic pathways. Our results imply that natural selection may drive rapid changes in the regulation of pheromones in urine among the different cat species, which in turn may influence social behavior, such as territorial marking and conspecific recognition, therefore serving as an important mechanism for the radiation of this group of mammals.
ESTHER : Li_2011_Mol.Biol.Evol_28_911
PubMedSearch : Li_2011_Mol.Biol.Evol_28_911
PubMedID: 20966115

Title : Complete genome sequence of Aeromonas veronii strain B565 - Li_2011_J.Bacteriol_193_3389
Author(s) : Li Y , Liu Y , Zhou Z , Huang H , Ren Y , Zhang Y , Li G , Wang L
Ref : Journal of Bacteriology , 193 :3389 , 2011
Abstract : Aeromonas veronii strain B565 was isolated from aquaculture pond sediment in China. We present here the complete genome sequence of B565 and compare it with 2 published genome sequences of pathogenic strains in the Aeromonas genus. The result represents an independent stepwise acquisition of virulence factors of pathogenic strains in this genus.
ESTHER : Li_2011_J.Bacteriol_193_3389
PubMedSearch : Li_2011_J.Bacteriol_193_3389
PubMedID: 21551296
Gene_locus related to this paper: aervb-f4d864 , aervb-f4d9b7 , 9gamm-u1fsk6 , aervb-f4dgq1

Title : Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4 - Janto_2011_Environ.Microbiol_13_3289
Author(s) : Janto B , Ahmed A , Ito M , Liu J , Hicks DB , Pagni S , Fackelmayer OJ , Smith TA , Earl J , Elbourne LD , Hassan K , Paulsen IT , Kolsto AB , Tourasse NJ , Ehrlich GD , Boissy R , Ivey DM , Li G , Xue Y , Ma Y , Hu FZ , Krulwich TA
Ref : Environ Microbiol , 13 :3289 , 2011
Abstract : Bacillus pseudofirmus OF4 is an extreme but facultative alkaliphile that grows non-fermentatively in a pH range from 7.5 to above 11.4 and can withstand large sudden increases in external pH. It is a model organism for studies of bioenergetics at high pH, at which energy demands are higher than at neutral pH because both cytoplasmic pH homeostasis and ATP synthesis require more energy. The alkaliphile also tolerates a cytoplasmic pH>9.0 at external pH values at which the pH homeostasis capacity is exceeded, and manages other stresses that are exacerbated at alkaline pH, e.g. sodium, oxidative and cell wall stresses. The genome of B.pseudofirmus OF4 includes two plasmids that are lost from some mutants without viability loss. The plasmids may provide a reservoir of mobile elements that promote adaptive chromosomal rearrangements under particular environmental conditions. The genome also reveals a more acidic pI profile for proteins exposed on the outer surface than found in neutralophiles. A large array of transporters and regulatory genes are predicted to protect the alkaliphile from its overlapping stresses. In addition, unanticipated metabolic versatility was observed, which could ensure requisite energy for alkaliphily under diverse conditions.
ESTHER : Janto_2011_Environ.Microbiol_13_3289
PubMedSearch : Janto_2011_Environ.Microbiol_13_3289
PubMedID: 21951522
Gene_locus related to this paper: alkpo-d3fst0

Title : Genomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation - Yang_2011_PLoS.Pathog_7_e1002179
Author(s) : Yang J , Wang L , Ji X , Feng Y , Li X , Zou C , Xu J , Ren Y , Mi Q , Wu J , Liu S , Liu Y , Huang X , Wang H , Niu X , Li J , Liang L , Luo Y , Ji K , Zhou W , Yu Z , Li G , Li L , Qiao M , Feng L , Zhang KQ
Ref : PLoS Pathog , 7 :e1002179 , 2011
Abstract : Nematode-trapping fungi are "carnivorous" and attack their hosts using specialized trapping devices. The morphological development of these traps is the key indicator of their switch from saprophytic to predacious lifestyles. Here, the genome of the nematode-trapping fungus Arthrobotrys oligospora Fres. (ATCC24927) was reported. The genome contains 40.07 Mb assembled sequence with 11,479 predicted genes. Comparative analysis showed that A. oligospora shared many more genes with pathogenic fungi than with non-pathogenic fungi. Specifically, compared to several sequenced ascomycete fungi, the A. oligospora genome has a larger number of pathogenicity-related genes in the subtilisin, cellulase, cellobiohydrolase, and pectinesterase gene families. Searching against the pathogen-host interaction gene database identified 398 homologous genes involved in pathogenicity in other fungi. The analysis of repetitive sequences provided evidence for repeat-induced point mutations in A. oligospora. Proteomic and quantitative PCR (qPCR) analyses revealed that 90 genes were significantly up-regulated at the early stage of trap-formation by nematode extracts and most of these genes were involved in translation, amino acid metabolism, carbohydrate metabolism, cell wall and membrane biogenesis. Based on the combined genomic, proteomic and qPCR data, a model for the formation of nematode trapping device in this fungus was proposed. In this model, multiple fungal signal transduction pathways are activated by its nematode prey to further regulate downstream genes associated with diverse cellular processes such as energy metabolism, biosynthesis of the cell wall and adhesive proteins, cell division, glycerol accumulation and peroxisome biogenesis. This study will facilitate the identification of pathogenicity-related genes and provide a broad foundation for understanding the molecular and evolutionary mechanisms underlying fungi-nematodes interactions.
ESTHER : Yang_2011_PLoS.Pathog_7_e1002179
PubMedSearch : Yang_2011_PLoS.Pathog_7_e1002179
PubMedID: 21909256
Gene_locus related to this paper: artoa-g1wyr4 , artoa-g1x1a7 , artoa-g1x3f4 , artoa-g1x3h6 , artoa-g1x9s5 , artoa-g1x9z4 , artoa-g1xcb5 , artoa-g1xhl6 , artoa-g1xjb3 , artoa-g1xjy0 , artoa-g1xkw3 , artoa-g1xnf2 , artoa-g1xnf8 , artoa-g1xqd4 , artoa-g1xqt1 , artoa-g1xte8 , artoa-g1xu91 , artoa-g1xv59 , artoa-g1x382 , artoa-g1x3q3 , artoa-g1wxl5 , artoa-g1xj75 , artoa-g1xd25 , artoa-g1wzu7 , artoa-g1xt42 , artoa-g1xhm8 , artoa-g1wy43

Title : Molecular cloning, overexpression and characterization of a novel feruloyl esterase from a soil metagenomic library - Sang_2011_J.Mol.Microbiol.Biotechnol_20_196
Author(s) : Sang SL , Li G , Hu XP , Liu YH
Ref : J Molecular Microbiology Biotechnol , 20 :196 , 2011
Abstract : The gene estF27, encoding a protein with feruloyl esterase activity, was cloned through functional screening from a soil metagenomic library and expressed in Escherichiacoli BL21 (DE3) with high solubility. Sequence analysis showed that estF27 encoded a protein of 291 amino acids with a predicted molecular mass of 31.16 kDa. According to the substrate specificity, EstF27 was classified as a type A feruloyl esterase. EstF27 displayed optimal activity at 40 degreesC and pH 6.8. This enzyme was stable in a broad pH range of 5.0-10.0 over 24 h, and retained more than 50% of its activity after 96 or 120 h incubation in the presence of 3 M KCl or 5 M NaCl. The enzyme activity was slightly enhanced by the addition of Mg(2+) and Fe(3+) at a low concentration, and completely inhibited by Cu(2+). In the enzymatic hydrolysis of destarched wheat bran, EstF27 could release ferulic acid from it in the presence of xylanase from Thermomyces lanuginosus. Given its alkalitolerance, halotolerance and highly soluble expression, EstF27 is a promising candidate for industrial applications.
ESTHER : Sang_2011_J.Mol.Microbiol.Biotechnol_20_196
PubMedSearch : Sang_2011_J.Mol.Microbiol.Biotechnol_20_196
PubMedID: 21757926
Gene_locus related to this paper: 9bact-e7djy5

Title : Genome sequence and analysis of the tuber crop potato - Xu_2011_Nature_475_189
Author(s) : Xu X , Pan S , Cheng S , Zhang B , Mu D , Ni P , Zhang G , Yang S , Li R , Wang J , Orjeda G , Guzman F , Torres M , Lozano R , Ponce O , Martinez D , De la Cruz G , Chakrabarti SK , Patil VU , Skryabin KG , Kuznetsov BB , Ravin NV , Kolganova TV , Beletsky AV , Mardanov AV , Di Genova A , Bolser DM , Martin DM , Li G , Yang Y , Kuang H , Hu Q , Xiong X , Bishop GJ , Sagredo B , Mejia N , Zagorski W , Gromadka R , Gawor J , Szczesny P , Huang S , Zhang Z , Liang C , He J , Li Y , He Y , Xu J , Zhang Y , Xie B , Du Y , Qu D , Bonierbale M , Ghislain M , Herrera Mdel R , Giuliano G , Pietrella M , Perrotta G , Facella P , O'Brien K , Feingold SE , Barreiro LE , Massa GA , Diambra L , Whitty BR , Vaillancourt B , Lin H , Massa AN , Geoffroy M , Lundback S , DellaPenna D , Buell CR , Sharma SK , Marshall DF , Waugh R , Bryan GJ , Destefanis M , Nagy I , Milbourne D , Thomson SJ , Fiers M , Jacobs JM , Nielsen KL , Sonderkaer M , Iovene M , Torres GA , Jiang J , Veilleux RE , Bachem CW , De Boer J , Borm T , Kloosterman B , van Eck H , Datema E , Hekkert B , Goverse A , van Ham RC , Visser RG
Ref : Nature , 475 :189 , 2011
Abstract : Potato (Solanum tuberosum L.) is the world's most important non-grain food crop and is central to global food security. It is clonally propagated, highly heterozygous, autotetraploid, and suffers acute inbreeding depression. Here we use a homozygous doubled-monoploid potato clone to sequence and assemble 86% of the 844-megabase genome. We predict 39,031 protein-coding genes and present evidence for at least two genome duplication events indicative of a palaeopolyploid origin. As the first genome sequence of an asterid, the potato genome reveals 2,642 genes specific to this large angiosperm clade. We also sequenced a heterozygous diploid clone and show that gene presence/absence variants and other potentially deleterious mutations occur frequently and are a likely cause of inbreeding depression. Gene family expansion, tissue-specific expression and recruitment of genes to new pathways contributed to the evolution of tuber development. The potato genome sequence provides a platform for genetic improvement of this vital crop.
ESTHER : Xu_2011_Nature_475_189
PubMedSearch : Xu_2011_Nature_475_189
PubMedID: 21743474
Gene_locus related to this paper: soltu-q2tqv0 , soltu-q4h433 , soltu-m0zl00 , soltu-m1aw23 , soltu-m0zxh5 , soltu-m1d3q4 , soltu-m1bz14 , soltu-m1d3q6 , sollc-k4b1g3 , soltu-m0zzn8 , soltu-m1ba60 , sollc-k4bf33 , soltu-m1c8d8 , soltu-m1ced9 , soltu-m1a385 , soltu-m1bz15 , soltu-m1a7s9 , soltu-m1bc84 , soltu-m1bpd1 , sollc-k4bm34 , soltu-m1a487 , soltu-m1a5u0 , soltu-m1cjx7 , soltu-m1bvq8 , soltu-m1baq1 , soltu-m1cfh4 , soltu-m1azl4 , soltu-m0ztj0 , soltu-m1d6d0 , soltu-m1cap1 , soltu-m1a7m1 , soltu-m1d3s6

Title : A thermostable esterase from Thermoanaerobacter tengcongensis opening up a new family of bacterial lipolytic enzymes - Rao_2011_Biochim.Biophys.Acta_1814_1695
Author(s) : Rao L , Xue Y , Zhou C , Tao J , Li G , Lu JR , Ma Y
Ref : Biochimica & Biophysica Acta , 1814 :1695 , 2011
Abstract : An unidentified alpha/beta hydrolase gene lipA3 from thermostable eubacterium species Thermoanaerobacter tengcongensis MB4 was cloned and heterologously expressed by Escherichia coli BL21(DE3)pLysS. The purified recombinant enzyme EstA3 turned out to be a monomeric thermostable esterase with optimal activity at 70degC and pH 9.5. The enzyme showed lipolytic activity towards a wide range of ester substrates including p-nitrophenyl esters and triacylglycerides, with the highest activity being observed for p-nitrophenyl caproate at 150 U/mg and for Triacetin at 126U/mg, respectively. Phylogenetic analysis revealed that EstA3 did not show homology to any identified bacterial lipolytic hydrolases. Sequence alignment showed that there was a common pentapeptide CHSMG with a cysteine replacing the first glycine in most esterase and lipase conserved motif GXSXG. The catalytic triad of EstA3 is Ser92, Asp269 and His292, which was confirmed by site directed mutagenesis. Based on the enzymatic properties and sequence alignment we concluded that the esterase EstA3 represented a novel bacterial lipolytic enzyme group and in chronological order this group was assigned as Family XIV.
ESTHER : Rao_2011_Biochim.Biophys.Acta_1814_1695
PubMedSearch : Rao_2011_Biochim.Biophys.Acta_1814_1695
PubMedID: 21907313
Gene_locus related to this paper: thete-LIPA3

Title : Sequence analysis and characterization of a transferable hybrid plasmid encoding multidrug resistance and enabling zoonotic potential for extraintestinal Escherichia coli - Johnson_2010_Infect.Immun_78_1931
Author(s) : Johnson TJ , Jordan D , Kariyawasam S , Stell AL , Bell NP , Wannemuehler YM , Alarcon CF , Li G , Tivendale KA , Logue CM , Nolan LK
Ref : Infect Immun , 78 :1931 , 2010
Abstract : ColV plasmids of extraintestinal pathogenic Escherichia coli (ExPEC) encode a variety of fitness and virulence factors and have long been associated with septicemia and avian colibacillosis. These plasmids are found significantly more often in ExPEC, including ExPEC associated with human neonatal meningitis and avian colibacillosis, than in commensal E. coli. Here we describe pAPEC-O103-ColBM, a hybrid RepFIIA/FIB plasmid harboring components of the ColV pathogenicity island and a multidrug resistance (MDR)-encoding island. This plasmid is mobilizable and confers the ability to cause septicemia in chickens, the ability to cause bacteremia resulting in meningitis in the rat model of human disease, and the ability to resist the killing effects of multiple antimicrobial agents and human serum. The results of a sequence analysis of this and other ColV plasmids supported previous findings which indicated that these plasmid types arose from a RepFIIA/FIB plasmid backbone on multiple occasions. Comparisons of pAPEC-O103-ColBM with other sequenced ColV and ColBM plasmids indicated that there is a core repertoire of virulence genes that might contribute to the ability of some ExPEC strains to cause high-level bacteremia and meningitis in a rat model. Examination of a neonatal meningitis E. coli (NMEC) population revealed that approximately 58% of the isolates examined harbored ColV-type plasmids and that 26% of these plasmids had genetic contents similar to that of pAPEC-O103-ColBM. The linkage of the ability to confer MDR and the ability contribute to multiple forms of human and animal disease on a single plasmid presents further challenges for preventing and treating ExPEC infections.
ESTHER : Johnson_2010_Infect.Immun_78_1931
PubMedSearch : Johnson_2010_Infect.Immun_78_1931
PubMedID: 20160015
Gene_locus related to this paper: ecoli-IROD , ecoli-IROE , ecoli-rdmC , ecoli-ypt1

Title : Balanced nuclear and cytoplasmic activities of EDS1 are required for a complete plant innate immune response - Garcia_2010_PLoS.Pathog_6_e1000970
Author(s) : Garcia AV , Blanvillain-Baufume S , Huibers RP , Wiermer M , Li G , Gobbato E , Rietz S , Parker JE
Ref : PLoS Pathog , 6 :e1000970 , 2010
Abstract : An important layer of plant innate immunity to host-adapted pathogens is conferred by intracellular nucleotide-binding/oligomerization domain-leucine rich repeat (NB-LRR) receptors recognizing specific microbial effectors. Signaling from activated receptors of the TIR (Toll/Interleukin-1 Receptor)-NB-LRR class converges on the nucleo-cytoplasmic immune regulator EDS1 (Enhanced Disease Susceptibility1). In this report we show that a receptor-stimulated increase in accumulation of nuclear EDS1 precedes or coincides with the EDS1-dependent induction and repression of defense-related genes. EDS1 is capable of nuclear transport receptor-mediated shuttling between the cytoplasm and nucleus. By enhancing EDS1 export from inside nuclei (through attachment of an additional nuclear export sequence (NES)) or conditionally releasing EDS1 to the nucleus (by fusion to a glucocorticoid receptor (GR)) in transgenic Arabidopsis we establish that the EDS1 nuclear pool is essential for resistance to biotrophic and hemi-biotrophic pathogens and for transcriptional reprogramming. Evidence points to post-transcriptional processes regulating receptor-triggered accumulation of EDS1 in nuclei. Changes in nuclear EDS1 levels become equilibrated with the cytoplasmic EDS1 pool and cytoplasmic EDS1 is needed for complete resistance and restriction of host cell death at infection sites. We propose that coordinated nuclear and cytoplasmic activities of EDS1 enable the plant to mount an appropriately balanced immune response to pathogen attack.
ESTHER : Garcia_2010_PLoS.Pathog_6_e1000970
PubMedSearch : Garcia_2010_PLoS.Pathog_6_e1000970
PubMedID: 20617163
Gene_locus related to this paper: arath-eds1

Title : Gene structure, recombinant expression and functional characterization of grass carp leptin - Li_2010_Gen.Comp.Endocrinol_166_117
Author(s) : Li GG , Liang XF , Xie Q , Li G , Yu Y , Lai K
Ref : General & Comparative Endocrinology , 166 :117 , 2010
Abstract : Leptin is an important hormone for the regulation of food intake, energy expenditure and reproduction in mammals, but information regarding its role in teleosts remains scant. In the present study, the gene structures of grass carp (Ctenopharyngodon idellus) and silver carp (Hypophthalmichthys molitrix) leptins were characterized. Recombinant grass carp leptin (rgc-LEP) was expressed in Escherichia coli and purified, and identified by mass spectrometric analysis. A strong anorexic effect on food intake was observed in grass carp on the first day after intraperitoneal (IP) injection of rgc-LEP, but not during the following days. Body weight of the leptin group (LEP group) and the pair-fed group (PF group) showed no difference throughout the experimental period. The acute and chronic effects on the expression of key genes correlating to food intake, energy expenditure, lipid metabolism and digestion were further characterized by real-time PCR. Accordingly, the mRNA levels of neuropeptide Y (NPY), Stearoyl-CoA desaturase 1 (SCD1) and lipoprotein lipase (LPL) were significantly reduced whereas the mRNA levels of uncoupling protein 2 (UCP2), bile salt-activated lipase (BSAL) and fatty acid elongase (ELO) were significantly elevated on the first day after injection. No effect on the expression of these genes (except LPL) was observed on day 13. In contrast to the down-regulation by exogenous leptin in mammals, the mRNA level of grass carp leptin was elevated 5.76-fold on the first day after rgc-LEP treatment. Our results suggest that leptin has an acute effect on the regulation of food intake, energy expenditure and lipid metabolism in grass carp, but the effect can be rapidly counteracted through mechanisms that are currently unknown.
ESTHER : Li_2010_Gen.Comp.Endocrinol_166_117
PubMedSearch : Li_2010_Gen.Comp.Endocrinol_166_117
PubMedID: 19857495

Title : The sequence and de novo assembly of the giant panda genome - Li_2010_Nature_463_311
Author(s) : Li R , Fan W , Tian G , Zhu H , He L , Cai J , Huang Q , Cai Q , Li B , Bai Y , Zhang Z , Zhang Y , Wang W , Li J , Wei F , Li H , Jian M , Nielsen R , Li D , Gu W , Yang Z , Xuan Z , Ryder OA , Leung FC , Zhou Y , Cao J , Sun X , Fu Y , Fang X , Guo X , Wang B , Hou R , Shen F , Mu B , Ni P , Lin R , Qian W , Wang G , Yu C , Nie W , Wang J , Wu Z , Liang H , Min J , Wu Q , Cheng S , Ruan J , Wang M , Shi Z , Wen M , Liu B , Ren X , Zheng H , Dong D , Cook K , Shan G , Zhang H , Kosiol C , Xie X , Lu Z , Li Y , Steiner CC , Lam TT , Lin S , Zhang Q , Li G , Tian J , Gong T , Liu H , Zhang D , Fang L , Ye C , Zhang J , Hu W , Xu A , Ren Y , Zhang G , Bruford MW , Li Q , Ma L , Guo Y , An N , Hu Y , Zheng Y , Shi Y , Li Z , Liu Q , Chen Y , Zhao J , Qu N , Zhao S , Tian F , Wang X , Wang H , Xu L , Liu X , Vinar T , Wang Y , Lam TW , Yiu SM , Liu S , Huang Y , Yang G , Jiang Z , Qin N , Li L , Bolund L , Kristiansen K , Wong GK , Olson M , Zhang X , Li S , Yang H
Ref : Nature , 463 :311 , 2010
Abstract : Using next-generation sequencing technology alone, we have successfully generated and assembled a draft sequence of the giant panda genome. The assembled contigs (2.25 gigabases (Gb)) cover approximately 94% of the whole genome, and the remaining gaps (0.05 Gb) seem to contain carnivore-specific repeats and tandem repeats. Comparisons with the dog and human showed that the panda genome has a lower divergence rate. The assessment of panda genes potentially underlying some of its unique traits indicated that its bamboo diet might be more dependent on its gut microbiome than its own genetic composition. We also identified more than 2.7 million heterozygous single nucleotide polymorphisms in the diploid genome. Our data and analyses provide a foundation for promoting mammalian genetic research, and demonstrate the feasibility for using next-generation sequencing technologies for accurate, cost-effective and rapid de novo assembly of large eukaryotic genomes.
ESTHER : Li_2010_Nature_463_311
PubMedSearch : Li_2010_Nature_463_311
PubMedID: 20010809
Gene_locus related to this paper: ailme-ABH15 , ailme-ACHE , ailme-BCHE , ailme-d2gtv3 , ailme-d2gty9 , ailme-d2gu87 , ailme-d2gu97 , ailme-d2gve7 , ailme-d2gwu1 , ailme-d2gx08 , ailme-d2gyt0 , ailme-d2gz36 , ailme-d2gz37 , ailme-d2gz38 , ailme-d2gz39 , ailme-d2gz40 , ailme-d2h5r9 , ailme-d2h7b7 , ailme-d2h9c9 , ailme-d2h794 , ailme-d2hau7 , ailme-d2hau8 , ailme-d2hcd9 , ailme-d2hdi6 , ailme-d2heu6 , ailme-d2hga4 , ailme-d2hqw5 , ailme-d2hs98 , ailme-d2hsx4 , ailme-d2hti6 , ailme-d2htv3 , ailme-d2htz6 , ailme-d2huc7 , ailme-d2hwj8 , ailme-d2hwy7 , ailme-d2hxm1 , ailme-d2hyc8 , ailme-d2hyv2 , ailme-d2hz11 , ailme-d2hza3 , ailme-d2hzr4 , ailme-d2i1l4 , ailme-d2i2g8 , ailme-g1l7m3 , ailme-g1lu36 , ailme-g1m769 , ailme-g1mc29 , ailme-g1mdj8 , ailme-g1mdr5 , ailme-g1mfp4 , ailme-g1mfx5 , ailme-g1lj41 , ailme-g1lm28 , ailme-g1l3u1 , ailme-g1l7l1 , ailme-g1m5i3 , ailme-g1l2f6 , ailme-g1lji5 , ailme-g1lqk3 , ailme-g1l8s9 , ailme-d2h717 , ailme-d2h718 , ailme-d2h719 , ailme-d2h720 , ailme-g1m5v0 , ailme-g1m5y7 , ailme-g1lkt7 , ailme-g1l2a1 , ailme-g1lsc8 , ailme-g1lrp4 , ailme-d2gv02 , ailme-g1mik5 , ailme-g1ljr1 , ailme-g1lxw7 , ailme-d2h8b5 , ailme-d2h2r2 , ailme-d2h9w7 , ailme-g1meh3 , ailme-g1m719

Title : A novel nicotinic acetylcholine receptor subtype in basal forebrain cholinergic neurons with high sensitivity to amyloid peptides - Liu_2009_J.Neurosci_29_918
Author(s) : Liu Q , Huang Y , Xue F , Simard AR , DeChon J , Li G , Zhang J , Lucero L , Wang M , Sierks M , Hu G , Chang Y , Lukas RJ , Wu J
Ref : Journal of Neuroscience , 29 :918 , 2009
Abstract : Nicotinic acetylcholine receptors (nAChRs) containing alpha7 subunits are thought to assemble as homomers. alpha7-nAChR function has been implicated in learning and memory, and alterations of alpha7-nAChR have been found in patients with Alzheimer's disease (AD). Here we report findings consistent with a novel, naturally occurring nAChR subtype in rodent, basal forebrain cholinergic neurons. In these cells, alpha7 subunits are coexpressed, colocalize, and coassemble with beta2 subunit(s). Compared with homomeric alpha7-nAChRs from ventral tegmental area neurons, functional, presumably heteromeric alpha7beta2-nAChRs on cholinergic neurons freshly dissociated from medial septum/diagonal band (MS/DB) exhibit relatively slow kinetics of whole-cell current responses to nicotinic agonists and are more sensitive to the beta2 subunit-containing nAChR-selective antagonist, dihydro-beta-erythroidine (DHbetaE). Interestingly, presumed, heteromeric alpha7beta2-nAChRs are highly sensitive to functional inhibition by pathologically relevant concentrations of oligomeric, but not monomeric or fibrillar, forms of amyloid beta(1-42) (Abeta(1-42)). Slow whole-cell current kinetics, sensitivity to DHbetaE, and specific antagonism by oligomeric Abeta(1-42) also are characteristics of heteromeric alpha7beta2-nAChRs, but not of homomeric alpha7-nAChRs, heterologously expressed in Xenopus oocytes. Moreover, choline-induced currents have faster kinetics and less sensitivity to Abeta when elicited from MS/DB neurons derived from nAChR beta2 subunit knock-out mice rather than from wild-type mice. The presence of novel, functional, heteromeric alpha7beta2-nAChRs on basal forebrain cholinergic neurons and their high sensitivity to blockade by low concentrations of oligomeric Abeta(1-42) suggests possible mechanisms for deficits in cholinergic signaling that could occur early in the etiopathogenesis of AD and might be targeted by disease therapies.
ESTHER : Liu_2009_J.Neurosci_29_918
PubMedSearch : Liu_2009_J.Neurosci_29_918
PubMedID: 19176801

Title : The genome of the cucumber, Cucumis sativus L - Huang_2009_Nat.Genet_41_1275
Author(s) : Huang S , Li R , Zhang Z , Li L , Gu X , Fan W , Lucas WJ , Wang X , Xie B , Ni P , Ren Y , Zhu H , Li J , Lin K , Jin W , Fei Z , Li G , Staub J , Kilian A , van der Vossen EA , Wu Y , Guo J , He J , Jia Z , Tian G , Lu Y , Ruan J , Qian W , Wang M , Huang Q , Li B , Xuan Z , Cao J , Asan , Wu Z , Zhang J , Cai Q , Bai Y , Zhao B , Han Y , Li Y , Li X , Wang S , Shi Q , Liu S , Cho WK , Kim JY , Xu Y , Heller-Uszynska K , Miao H , Cheng Z , Zhang S , Wu J , Yang Y , Kang H , Li M , Liang H , Ren X , Shi Z , Wen M , Jian M , Yang H , Zhang G , Yang Z , Chen R , Ma L , Liu H , Zhou Y , Zhao J , Fang X , Fang L , Liu D , Zheng H , Zhang Y , Qin N , Li Z , Yang G , Yang S , Bolund L , Kristiansen K , Li S , Zhang X , Wang J , Sun R , Zhang B , Jiang S , Du Y
Ref : Nat Genet , 41 :1275 , 2009
Abstract : Cucumber is an economically important crop as well as a model system for sex determination studies and plant vascular biology. Here we report the draft genome sequence of Cucumis sativus var. sativus L., assembled using a novel combination of traditional Sanger and next-generation Illumina GA sequencing technologies to obtain 72.2-fold genome coverage. The absence of recent whole-genome duplication, along with the presence of few tandem duplications, explains the small number of genes in the cucumber. Our study establishes that five of the cucumber's seven chromosomes arose from fusions of ten ancestral chromosomes after divergence from Cucumis melo. The sequenced cucumber genome affords insight into traits such as its sex expression, disease resistance, biosynthesis of cucurbitacin and 'fresh green' odor. We also identify 686 gene clusters related to phloem function. The cucumber genome provides a valuable resource for developing elite cultivars and for studying the evolution and function of the plant vascular system.
ESTHER : Huang_2009_Nat.Genet_41_1275
PubMedSearch : Huang_2009_Nat.Genet_41_1275
PubMedID: 19881527
Gene_locus related to this paper: cucsa-a0a0a0ktw5 , cucsa-a0a0a0lnt6 , cucsa-a0a0a0kpn7 , cucsa-a0a0a0lvt9 , cucsa-a0a0a0kdx8 , cucsa-a0a0a0m228 , cucsa-a0a0a0kz31 , cucsa-a0a0a0k5t5 , cucsa-a0a0a0kfs7 , cucsa-a0a0a0kjj7 , cucsa-a0a0a0kzs7 , cucsa-a0a0a0l0a6 , cucsa-a0a0a0l4w4 , cucsa-a0a0a0lpz0 , cucsa-a0a0a0ls66

Title : Molecular cloning and characterization of a novel pyrethroid-hydrolyzing esterase originating from the Metagenome - Li_2008_Microb.Cell.Fact_7_38
Author(s) : Li G , Wang K , Liu YH
Ref : Microb Cell Fact , 7 :38 , 2008
Abstract : BACKGROUND: Pyrethroids and pyrethrins are widely used insecticides. Extensive applications not only result in pest resistance to these insecticides, but also may lead to environmental issues and human exposure. Numerous studies have shown that very high exposure to pyrethroids might cause potential problems to man and aquatic organisms. Therefore, it is important to develop a rapid and efficient disposal process to eliminate or minimize contamination of surface water, groundwater and agricultural products by pyrethroid insecticides. Bioremediation is considered to be a reliable and cost-effective technique for pesticides abatement and a major factor determining the fate of pyrethroid pesticides in the environment, and suitable esterase is expected to be useful for potential application for detoxification of pyrethroid residues. Soil is a complex environment considered as one of the main reservoirs of microbial diversity on the planet. However, most of the microorganisms in nature are inaccessible as they are uncultivable in the laboratory. Metagenomic approaches provide a powerful tool for accessing novel valuable genetic resources (novel enzymes) and developing various biotechnological applications.
RESULTS: The pyrethroid pesticides residues on foods and the environmental contamination are a public safety concern. Pretreatment with pyrethroid-hydrolyzing esterase has the potential to alleviate the conditions. To this end, a pyrethroid-hydrolyzing esterase gene was successfully cloned using metagenomic DNA combined with activity-based functional screening from soil, sequence analysis of the DNA responsible for the pye3 gene revealed an open reading frame of 819 bp encoding for a protein of 272 amino acid residues. Extensive multiple sequence alignments of the deduced amino acid of Pye3 with the most homologous carboxylesterases revealed moderate identity (45-49%). The recombinant Pye3 was heterologously expressed in E. coli BL21(DE3), purified and characterized. The molecular mass of the native enzyme was approximately 31 kDa as determined by gel filtration. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the deduced amino acid sequence of the Pye3 indicated molecular mass of 31 kDa and 31.5 kDa, respectively, suggesting that the Pye3 is a monomer. The purified Pye3 not only degraded all pyrethroid pesticides tested, but also hydrolyzed rho-nitrophenyl esters of medium-short chain fatty acids, indicating that the Pye3 is an esterase with broader specificity. The Km values for trans-Permethrin and cis-permethrin are 0.10 muM and 0.18 muM, respectively, and these catalytic properties were superior to carboxylesterases from resistant insects and mammals. The catalytic activity of the Pye3 was strongly inhibited by Hg2+, Ag+, rho-chloromercuribenzoate, whereas less pronounced effect was observed in the presence of divalent cations, the chelating agent EDTA and phenanthroline. CONCLUSION: A novel pyrethroid-hydrolyzing esterase gene was successfully cloned using metagenomic DNA combined with activity-based functional screening from soil, the broader substrate specificities and higher activity of the pyrethroid-hydrolyzing esterase (Pye3) make it an ideal candidate for in situ for detoxification of pyrethroids where they cause environmental contamination problems. Consequently, metagenomic DNA clone library offers possibilities to discover novel bio-molecules through the expression of genes from uncultivated bacteria.
ESTHER : Li_2008_Microb.Cell.Fact_7_38
PubMedSearch : Li_2008_Microb.Cell.Fact_7_38
PubMedID: 19116015
Gene_locus related to this paper: 9zzzz-b6vg94

Title : Mapping and expression analysis of chicken NDRG1 and NDRG3 genes - Tian_2008_Biochem.Genet_46_677
Author(s) : Tian Y , Xu M , Fu Y , Yuan A , Wang D , Li G , Liu G , Lu L
Ref : Biochemical Genetics , 46 :677 , 2008
Abstract : N-myc downstream-regulated genes 1 and 3 (NDRG1 and NDRG3) are members of the alpha/beta hydrolase superfamily. Phylogenetic analysis of the family demonstrated that human NDRG1 and 3 belong to a subfamily. The mapping and gene expression patterns of these genes represent one step toward further investigation into their possible roles in the chicken (Gallus gallus). To map these genes in the chicken chromosome, a 6000 rads chicken-hamster radiation hybrid panel (ChickRH6) was used. Primers were designed according to the published human sequences for amplification of those two genes. We compared the corresponding human mRNA sequences with the predicted coding sequences of the chicken NDRG1 and 3 genes and found that the assembled contigs shared a high percentage of similarity with the human genes. PCR of samples from ChickRH6 revealed that the locations of NDRG1 and 3 are linked to the markers MYC (58 cRs away, LOD score 4.52) and SEQ0265 (10 cRs away, LOD score 17.81), respectively. This result adds two new markers to the chicken RH map, and it reinforces that the RH technique is indeed a powerful tool for mapping genes due to its rapidity, precision, convenience, and reproducibility. In addition, we detected the gene expression and distribution of chicken NDRG1 and 3 in seven tissues, including heart, liver, spleen, lung, muscle, brain, and thymus, by RT-PCR, and found that NDRG1 is relatively ubiquitously expressed in all the tested tissues and highly expressed in heart and liver, whereas NDRG3 is high in heart, muscle, and brain.
ESTHER : Tian_2008_Biochem.Genet_46_677
PubMedSearch : Tian_2008_Biochem.Genet_46_677
PubMedID: 18751885

Title : New insight into the mechanism of methyl transfer during the biosynthesis of fosfomycin - Woodyer_2007_Chem.Commun.(Camb)__359
Author(s) : Woodyer RD , Li G , Zhao H , van der Donk WA
Ref : Chem Commun (Camb) , :359 , 2007
Abstract : Hydroxyethylphosphonate is a required intermediate in fosfomycin biosynthesis.
ESTHER : Woodyer_2007_Chem.Commun.(Camb)__359
PubMedSearch : Woodyer_2007_Chem.Commun.(Camb)__359
PubMedID: 17220970

Title : Protective effects of Hippophae rhamnoides L. juice on lead-induced neurotoxicity in mice - Xu_2005_Biol.Pharm.Bull_28_490
Author(s) : Xu Y , Li G , Han C , Sun L , Zhao R , Cui S
Ref : Biol Pharm Bull , 28 :490 , 2005
Abstract : We examined the effect of Hippophae rhamnoides L. (HRL) juice on lead-induced memory impairment and neuronal damage in the brains of adult mice. Kunming mice were exposed to lead acetate 10 mg/kg body weight for 20 d. Twenty percent and 40% HRL prevented the lead-induced decrease in step-through latency. In the water maze test, the swimming time was lengthened in mice treated with lead acetate, but this time was decreased in mice that received 20% and 40% HRL. The malondialdehyde (MDA) levels were increased in lead-treated mice, which were reduced by 20% and 40% HRL in dose-dependent manner. The activities of acetylcholinesterase (AchE) and monoamine oxidase-A and -B were significantly increased in the lead-treated group, which were decreased by 40% HRL but not by 20% HRL. The levels of norepinephrine, serotonin, and 5-hydroxyindole acetic acid were decreased significantly in the lead-treated mice, and the decreases were antagonized by 40% HRL, except for than in dopamine, but 20% HRL had no effect on this change. These data suggest that the different doses of the HRL juice protect against the lead acetate-induced deficits in learning and memory and changes in neurobiochemical parameters.
ESTHER : Xu_2005_Biol.Pharm.Bull_28_490
PubMedSearch : Xu_2005_Biol.Pharm.Bull_28_490
PubMedID: 15744075

Title : The Genomes of Oryza sativa: a history of duplications - Yu_2005_PLoS.Biol_3_e38
Author(s) : Yu J , Wang J , Lin W , Li S , Li H , Zhou J , Ni P , Dong W , Hu S , Zeng C , Zhang J , Zhang Y , Li R , Xu Z , Li X , Zheng H , Cong L , Lin L , Yin J , Geng J , Li G , Shi J , Liu J , Lv H , Li J , Deng Y , Ran L , Shi X , Wang X , Wu Q , Li C , Ren X , Li D , Liu D , Zhang X , Ji Z , Zhao W , Sun Y , Zhang Z , Bao J , Han Y , Dong L , Ji J , Chen P , Wu S , Xiao Y , Bu D , Tan J , Yang L , Ye C , Xu J , Zhou Y , Yu Y , Zhang B , Zhuang S , Wei H , Liu B , Lei M , Yu H , Li Y , Xu H , Wei S , He X , Fang L , Huang X , Su Z , Tong W , Tong Z , Ye J , Wang L , Lei T , Chen C , Chen H , Huang H , Zhang F , Li N , Zhao C , Huang Y , Li L , Xi Y , Qi Q , Li W , Hu W , Tian X , Jiao Y , Liang X , Jin J , Gao L , Zheng W , Hao B , Liu S , Wang W , Yuan L , Cao M , McDermott J , Samudrala R , Wong GK , Yang H
Ref : PLoS Biol , 3 :e38 , 2005
Abstract : We report improved whole-genome shotgun sequences for the genomes of indica and japonica rice, both with multimegabase contiguity, or almost 1,000-fold improvement over the drafts of 2002. Tested against a nonredundant collection of 19,079 full-length cDNAs, 97.7% of the genes are aligned, without fragmentation, to the mapped super-scaffolds of one or the other genome. We introduce a gene identification procedure for plants that does not rely on similarity to known genes to remove erroneous predictions resulting from transposable elements. Using the available EST data to adjust for residual errors in the predictions, the estimated gene count is at least 38,000-40,000. Only 2%-3% of the genes are unique to any one subspecies, comparable to the amount of sequence that might still be missing. Despite this lack of variation in gene content, there is enormous variation in the intergenic regions. At least a quarter of the two sequences could not be aligned, and where they could be aligned, single nucleotide polymorphism (SNP) rates varied from as little as 3.0 SNP/kb in the coding regions to 27.6 SNP/kb in the transposable elements. A more inclusive new approach for analyzing duplication history is introduced here. It reveals an ancient whole-genome duplication, a recent segmental duplication on Chromosomes 11 and 12, and massive ongoing individual gene duplications. We find 18 distinct pairs of duplicated segments that cover 65.7% of the genome; 17 of these pairs date back to a common time before the divergence of the grasses. More important, ongoing individual gene duplications provide a never-ending source of raw material for gene genesis and are major contributors to the differences between members of the grass family.
ESTHER : Yu_2005_PLoS.Biol_3_e38
PubMedSearch : Yu_2005_PLoS.Biol_3_e38
PubMedID: 15685292
Gene_locus related to this paper: orysa-Q7XTC5 , orysa-Q852M6 , orysa-Q8GSE8 , orysa-Q9S7P1 , orysa-Q9FYP7 , orysa-Q5ZBH3 , orysa-Q5ZA26 , orysa-Q5JLP6 , orysa-Q8H5P9 , orysa-Q8H5P5 , orysa-Q7F1Y5 , orysa-Q949C9 , orysa-cbp1 , orysa-cbp3 , orysa-cbpx , orysa-Q33B71 , orysa-Q8GSJ3 , orysa-LPL1 , orysa-Q6YSZ8 , orysa-Q8S5X5 , orysa-Q8LIG3 , orysa-Q6K7F5 , orysa-Q7F1B1 , orysa-Q8H4S9 , orysa-Q69UB1 , orysa-Q9FW17 , orysa-Q337C3 , orysa-Q7F959 , orysa-Q84QZ6 , orysa-Q84QY7 , orysa-Q851E3 , orysa-Q6YTH5 , orysa-Q0JK71 , orysa-Q8S1D9 , orysa-Q5N8V4 , orysa-Q0JCY4 , orysa-Q8GTK2 , orysa-B9EWJ8 , orysa-Q8H3K6 , orysa-Q6ZDG8 , orysa-Q6ZDG6 , orysa-Q6ZDG5 , orysa-Q6ZDG4 , orysa-Q5NAI4 , orysa-Q658B2 , orysa-Q5JMQ8 , orysa-Q5QMD9 , orysa-Q5N7L1 , orysa-Q8RYV9 , orysa-Q8H3R3 , orysa-Q5SNH3 , orysa-Q8W0F0 , orysa-pir7a , orysa-pir7b , orysa-q2qlm4 , orysa-q2qm78 , orysa-q2qm82 , orysa-q2qn31 , orysa-q2qnj4 , orysa-q2qnt9 , orysa-q2qur1 , orysa-q2qx94 , orysa-q2qyi1 , orysa-q2qyj1 , orysa-q2r051 , orysa-q2r077 , orysa-q2ram0 , orysa-q2rat1 , orysa-q2rbb3 , orysa-Q4VWY7 , orysa-q5na00 , orysa-q5nbu1 , orysa-Q5QLC0 , orysa-q5smv5 , orysa-Q5VP27 , orysa-q5vrt2 , orysa-q5w6c5 , orysa-q5z5a3 , orysa-q5z9i2 , orysa-q5z417 , orysa-q5z901 , orysa-Q5ZAM8 , orysa-Q5ZBI5 , orysa-Q5ZCR3 , orysa-q6atz0 , orysa-q6ave2 , orysa-q6f358 , orysa-q6h6s1 , orysa-q6h7i6 , orysa-q6i5q3 , orysa-q6i5u7 , orysa-q6j657 , orysa-q6k3d9 , orysa-q6k4q2 , orysa-q6k880 , orysa-q6l5b6 , orysa-Q6L5F5 , orysa-q6l556 , orysj-q6yse8 , orysa-q6yy42 , orysa-q6yzk1 , orysa-q6z8b1 , orysa-q6z995 , orysa-q6zc62 , orysa-q6zia4 , orysa-q6zjq6 , orysa-q7x7y5 , orysa-Q7XC50 , orysa-q7xej4 , orysa-q7xem8 , orysa-q7xkj9 , orysa-q7xr62 , orysa-q7xr63 , orysa-q7xr64 , orysa-q7xsg1 , orysa-q7xsq2 , orysa-q7xts6 , orysa-q7xv53 , orysa-Q7XVB5 , orysa-Q8L562 , orysa-Q8LQS5 , orysa-Q8RZ40 , orysa-Q8RZ79 , orysa-Q8S0U8 , orysa-Q8S0V0 , orysa-Q8S125 , orysa-Q8SAY7 , orysa-Q8SAY9 , orysa-Q8W3C6 , orysa-Q8W3F2 , orysa-Q8W3F4 , orysa-Q8W3F6 , orysa-Q9LHX5 , orysa-q33aq0 , orysa-q53lh1 , orysa-q53m20 , orysa-q53nd8 , orysa-q60e79 , orysa-q60ew8 , orysa-q67iz2 , orysa-q67iz3 , orysa-q67iz7 , orysa-q67iz8 , orysa-q67j02 , orysa-q67j05 , orysa-q67j07 , orysa-q67j09 , orysa-q67j10 , orysa-q67tr6 , orysa-q67tv0 , orysa-q67uz1 , orysa-q67v34 , orysa-q67wz5 , orysa-q69j38 , orysa-q69k08 , orysa-q69md7 , orysa-q69me0 , orysa-q69pf3 , orysa-q69ti3 , orysa-q69xr2 , orysa-q69y12 , orysa-q69y21 , orysa-q75hy2 , orysa-q75i01 , orysa-Q94JD7 , orysa-Q0J0A4 , orysa-q651a8 , orysa-q651z3 , orysa-q652g4 , orysa-q688m0 , orysa-q688m8 , orysa-q688m9 , orysa-Q6H8G1 , orysi-a2wn01 , orysi-a2xc83 , orysi-a2yh83 , orysi-a2z179 , orysi-a2zef2 , orysi-b8a7e6 , orysi-b8a7e7 , orysi-b8bfe5 , orysi-b8bhp9 , orysj-a3b9l8 , orysj-b9eub8 , orysj-b9eya5 , orysj-b9fi05 , orysj-b9fkb0 , orysj-b9fn42 , orysj-b9gbb7 , orysj-cgep , orysj-PLA7 , orysj-q0d4u5 , orysj-q0djj0 , orysj-q0jaf0 , orysj-q5jl22 , orysj-q5jlw7 , orysj-q5z419 , orysj-q6h7q9 , orysj-q6yvk6 , orysj-q6z6i1 , orysj-q7f8x1 , orysj-q7xcx3 , orysj-q9fwm6 , orysj-q10j20 , orysj-q10ss2 , orysj-q69uw6 , orysj-q94d71 , orysj-q338c0 , orysi-b8bly4 , orysj-b9gbs4 , orysi-a2zb88 , orysj-b9gbs1 , orysi-b8b698 , orysj-pla4 , orysj-pla1

Title : Lipase-mediated stereoselective hydrolysis of stampidine and other phosphoramidate derivatives of stavudine - Venkatachalam_2004_Bioorg.Med.Chem_12_3371
Author(s) : Venkatachalam TK , Samuel P , Li G , Qazi S , Mao C , Pendergrass S , Uckun FM
Ref : Bioorganic & Medicinal Chemistry , 12 :3371 , 2004
Abstract : Enzymatic hydrolysis of stampidine and other aryl phosphate derivatives of stavudine were investigated using the Candida Antarctica Type B lipase. Modeling studies and comparison of the hydrolysis rate constants revealed a chiral preference of the lipase active site for the putative S-stereoisomer. The in vitro anti-HIV activity of these compounds correlated with their susceptibility to lipase- (but not esterase-) mediated hydrolysis. We propose that stampidine undergoes rapid enzymatic hydrolysis in the presence of lipase according to the following biochemical pathway: During the first step, hydrolysis of the ester group results in the formation of carboxylic acid. Subsequent step involves an intramolecular cyclization at the phosphorous center with simultaneous elimination of the phenoxy group to form a cyclic intermediate. In the presence of water, this intermediate is converted into the active metabolite Ala-d4T-MP. We postulate that the lipase hydrolyzes the methyl ester group of the l-alanine side chain to form the cyclic intermediate in a stereoselective fashion. This hypothesis was supported by experimental data showing that chloroethyl substituted derivatives of stampidine, which possess a chloroethyl linker unit instead of a methyl ester side chain, were resistant to lipase-mediated hydrolysis, which excludes the possibility of a direct hydrolysis of stampidine at the phosphorous center. Thus, our model implies that the lipase-mediated formation of the cyclic intermediate is a key step in metabolism of stampidine and relies on the initial configuration of the stereoisomers.
ESTHER : Venkatachalam_2004_Bioorg.Med.Chem_12_3371
PubMedSearch : Venkatachalam_2004_Bioorg.Med.Chem_12_3371
PubMedID: 15158806

Title : The influence of aging on whole body choline release and clearance - Jenden_1996_Life.Sci_58(22)_2003
Author(s) : Jenden DJ , Scremin OU , Roch M , Li G
Ref : Life Sciences , 58 :2003 , 1996
Abstract : We have confirmed that hypoxia elicits a substantial rise in blood choline levels in young adult rats. An intravenous infusion of tracer quantities of [2H4]-Ch, serial measurements of blood [2H0]-Ch and [2H4]-Ch, and a simple pharmacokinetic model were used to assess the bidirectional flux of choline between the central pool and peripheral pools before, during and after a period of imposed hypoxia, in rats ranging from 56 to 780 days of age. The results indicate that the age-dependence of the hypercholinemic response to hypoxia is predominantly due to an increase in the amount of choline released in response to hypoxia, and that changes in its clearance are relatively unimportant.
ESTHER : Jenden_1996_Life.Sci_58(22)_2003
PubMedSearch : Jenden_1996_Life.Sci_58(22)_2003
PubMedID: 8637430

Title : Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease. -
Author(s) : Sherrington R , Rogaev EI , Liang Y , Rogaeva EA , Levesque G , Ikeda M , Chi H , Lin C , Li G , Holman K , Tsuda T , Mar L , Foncin J-F , Bruni AC , Montesi MP , Sorbi S , Rainero I , Pinessi L , Nee L , Chumakov I , Pollen D , Brookes A , Sanseau P , Polinsky RJ , Wasco W , da Silva HAR , Haines JL , Pericak-Vance MA , Tanzi RE , Roses AD , Fraser PE , Rommens JM , St George-Hyslop PH
Ref : Nature , 375 :754 , 1995
PubMedID: 7596406
Gene_locus related to this paper: human-ACOT2