Xing_2001_Sheng.Wu.Gong.Cheng.Xue.Bao_17_199

Reference

Title : [Cloning and high level expression of mosquito detoxifying gene] - Xing_2001_Sheng.Wu.Gong.Cheng.Xue.Bao_17_199
Author(s) : Xing JM , Qiao CL , Huang J , Li X , Jia XD , Li DM
Ref : Sheng Wu Gong Cheng Xue Bao , 17 :199 , 2001
Abstract :

The full-length cDNA of mosquito esterase B1 had been isolated, and subcloned into pBV220. The recombinant vector pBV220B1 was constructed and transformed into E. coli DH5 alpha. A 60 kD protein was induced by 42 degrees C and its expression was temperature-dependent. After 6 h induction, the target protein occupied 50% of the total protein. The expressed product existed in both inclusion body and soluble proteins in the cells. The amount of the soluble detoxifying enzyme increased along with the induction time. The data of detoxifying experiments indicated that the detoxifying enzyme in expression strain of E. coli can detoxified toxicity of organophosphate insecticides, it showed a clear detoxifying affect on hens poisoned by organophosphate insecticides.

PubMedSearch : Xing_2001_Sheng.Wu.Gong.Cheng.Xue.Bao_17_199
PubMedID: 11411231

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Citations formats

Xing JM, Qiao CL, Huang J, Li X, Jia XD, Li DM (2001)
[Cloning and high level expression of mosquito detoxifying gene]
Sheng Wu Gong Cheng Xue Bao 17 :199

Xing JM, Qiao CL, Huang J, Li X, Jia XD, Li DM (2001)
Sheng Wu Gong Cheng Xue Bao 17 :199