Xu_2017_Comp.Biochem.Physiol.B.Biochem.Mol.Biol_205_46

Reference

Title : Molecular cloning, characterization and expression analysis of two juvenile hormone esterase-like carboxylesterase cDNAs in Chinese mitten crab, Eriocheir sinensis - Xu_2017_Comp.Biochem.Physiol.B.Biochem.Mol.Biol_205_46
Author(s) : Xu Y , Zhao M , Deng Y , Yang Y , Li X , Lu Q , Ge J , Pan J , Xu Z
Ref : Comparative Biochemistry & Physiology B Biochem Mol Biol , 205 :46 , 2017
Abstract :

Precise regulation of methyl farnesoate (MF) titer is of prime importance throughout the crustacean life-cycle. Although the synthetic pathway of MF is well-documented, little is known about its degradation and recycling in crustaceans. Juvenile hormone esterase-like (JHE-like) carboxylesterase (CXE) is a key enzyme in MF degradation, thus playing a significant role in regulating the MF titer. We identified and characterized two cDNAs, Es-CXE1 and Es-CXE2, encoding JHE-like CXEs in Chinese mitten crab. Full-length cDNAs of Es-CXE1 and Es-CXE2 encode proteins composed of 584 and 597 amino acids, respectively, both of which contain a typical carboxylesterase domain. Alignment and phylogenetic analyses revealed that the Es-CXEs are highly similar to those of other crustaceans. To further validate their functions, we evaluated the mRNA expression patterns of the Es-CXEs in various tissues and in different physiological conditions. Tissue-specific expression analysis showed that the two Es-CXEs were predominantly expressed in the hepatopancreas and ovaries, which are the major tissues for MF metabolism. Es-CXE2 expression levels in the hepatopancreas and ovaries were about 100 and 25-fold higher, than the respective Es-CXE1 expressions. During ovarian rapid development stage, the global expressions of Es-CXEs were up-regulated in the hepatopancreas and down-regulated in the ovaries. After eyestalk ablation (ESA), the mRNA expressions of the two Es-CXEs were up-regulated in the hepatopancreas, further indicating their potential in degrading MF. Taken together, our results suggest that Es-CXEs, the key component of the juvenile hormone degradation pathway, may play vital roles in the development and reproduction of the Chinese mitten crab.

PubMedSearch : Xu_2017_Comp.Biochem.Physiol.B.Biochem.Mol.Biol_205_46
PubMedID: 28077333
Gene_locus related to this paper: erisi-a0a1l5jht6

Related information

Substrate Methyl-farnesoate
Gene_locus erisi-a0a1l5jht6
Gene_locus_frgt erisi-a0a1l5jhu7

Citations formats

Xu Y, Zhao M, Deng Y, Yang Y, Li X, Lu Q, Ge J, Pan J, Xu Z (2017)
Molecular cloning, characterization and expression analysis of two juvenile hormone esterase-like carboxylesterase cDNAs in Chinese mitten crab, Eriocheir sinensis
Comparative Biochemistry & Physiology B Biochem Mol Biol 205 :46

Xu Y, Zhao M, Deng Y, Yang Y, Li X, Lu Q, Ge J, Pan J, Xu Z (2017)
Comparative Biochemistry & Physiology B Biochem Mol Biol 205 :46