Zedelius_2011_Environ.Microbiol.Rep_3_125

Reference

Title : Alkane degradation under anoxic conditions by a nitrate-reducing bacterium with possible involvement of the electron acceptor in substrate activation - Zedelius_2011_Environ.Microbiol.Rep_3_125
Author(s) : Zedelius J , Rabus R , Grundmann O , Werner I , Brodkorb D , Schreiber F , Ehrenreich P , Behrends A , Wilkes H , Kube M , Reinhardt R , Widdel F
Ref : Environ Microbiol Rep , 3 :125 , 2011
Abstract :

Microorganisms can degrade saturated hydrocarbons (alkanes) not only under oxic but also under anoxic conditions. Three denitrifying isolates (strains HxN1, OcN1, HdN1) able to grow under anoxic conditions by coupling alkane oxidation to CO(2) with NO(3) (-) reduction to N(2) were compared with respect to their alkane metabolism. Strains HxN1 and OcN1, which are both Betaproteobacteria, utilized n-alkanes from C(6) to C(8) and C(8) to C(12) respectively. Both activate alkanes anaerobically in a fumarate-dependent reaction yielding alkylsuccinates, as suggested by present and previous metabolite and gene analyses. However, strain HdN1 was unique in several respects. It belongs to the Gammaproteobacteria and was more versatile towards alkanes, utilizing the range from C(6) to C(30). Neither analysis of metabolites nor analysis of genes in the complete genome sequence of strain HdN1 hinted at fumarate-dependent alkane activation. Moreover, whereas strains HxN1 and OcN1 grew with alkanes and NO(3) (-), NO(2) (-) or N(2)O added to the medium, strain HdN1 oxidized alkanes only with NO(3) (-) or NO(2) (-) but not with added N(2)O; but N(2)O was readily used for growth with long-chain alcohols or fatty acids. Results suggest that NO(2) (-) or a subsequently formed nitrogen compound other than N(2)O is needed for alkane activation in strain HdN1. From an energetic point of view, nitrogen-oxygen species are generally rather strong oxidants. They may enable enzymatic mechanisms that are not possible under conditions of sulfate reduction or methanogenesis and thus allow a special mode of alkane activation.

PubMedSearch : Zedelius_2011_Environ.Microbiol.Rep_3_125
PubMedID: 21837252
Gene_locus related to this paper: 9gamm-e1vi37 , 9gamm-e1vjh4 , 9gamm-e1vli3 , 9gamm-e1vqy3 , 9gamm-e1vnt0 , 9gamm-e1vj65 , 9gamm-e1vpf8

Related information

Gene_locus 9gamm-e1vi37    9gamm-e1vjh4    9gamm-e1vli3    9gamm-e1vqy3    9gamm-e1vnt0    9gamm-e1vj65    9gamm-e1vpf8
Gene_locus_frgt 9gamm-e1vi37    9gamm-e1vjh4    9gamm-e1vli3    9gamm-e1vqy3    9gamm-e1vnt0    9gamm-e1vj65    9gamm-e1vpf8    9gamm-e1vq62

Citations formats

Zedelius J, Rabus R, Grundmann O, Werner I, Brodkorb D, Schreiber F, Ehrenreich P, Behrends A, Wilkes H, Kube M, Reinhardt R, Widdel F (2011)
Alkane degradation under anoxic conditions by a nitrate-reducing bacterium with possible involvement of the electron acceptor in substrate activation
Environ Microbiol Rep 3 :125

Zedelius J, Rabus R, Grundmann O, Werner I, Brodkorb D, Schreiber F, Ehrenreich P, Behrends A, Wilkes H, Kube M, Reinhardt R, Widdel F (2011)
Environ Microbiol Rep 3 :125