de Saint Germain_2016_Nat.Chem.Biol_12_787

Reference

Title : An histidine covalent receptor and butenolide complex mediates strigolactone perception - de Saint Germain_2016_Nat.Chem.Biol_12_787
Author(s) : de Saint Germain A , Clave G , Badet-Denisot MA , Pillot JP , Cornu D , Le Caer JP , Burger M , Pelissier F , Retailleau P , Turnbull C , Bonhomme S , Chory J , Rameau C , Boyer FD
Ref : Nat Chemical Biology , 12 :787 , 2016
Abstract :

Strigolactone plant hormones control plant architecture and are key players in both symbiotic and parasitic interactions. They contain an ABC tricyclic lactone connected to a butenolide group, the D ring. The DWARF14 (D14) strigolactone receptor belongs to the superfamily of alpha/beta-hydrolases, and is known to hydrolyze the bond between the ABC lactone and the D ring. Here we characterized the binding and catalytic functions of RAMOSUS3 (RMS3), the pea (Pisum sativum) ortholog of rice (Oryza sativa) D14 strigolactone receptor. Using new profluorescent probes with strigolactone-like bioactivity, we found that RMS3 acts as a single-turnover enzyme that explains its apparent low enzymatic rate. We demonstrated the formation of a covalent RMS3-D-ring complex, essential for bioactivity, in which the D ring was attached to histidine 247 of the catalytic triad. These results reveal an undescribed mechanism of plant hormone reception in which the receptor performs an irreversible enzymatic reaction to generate its own ligand.

PubMedSearch : de Saint Germain_2016_Nat.Chem.Biol_12_787
PubMedID: 27479744
Gene_locus related to this paper: pea-RMS3

Related information

Substrate GC242    GC240    GC486
Gene_locus GC242    GC240    GC486    pea-RMS3

Citations formats

de Saint Germain A, Clave G, Badet-Denisot MA, Pillot JP, Cornu D, Le Caer JP, Burger M, Pelissier F, Retailleau P, Turnbull C, Bonhomme S, Chory J, Rameau C, Boyer FD (2016)
An histidine covalent receptor and butenolide complex mediates strigolactone perception
Nat Chemical Biology 12 :787

de Saint Germain A, Clave G, Badet-Denisot MA, Pillot JP, Cornu D, Le Caer JP, Burger M, Pelissier F, Retailleau P, Turnbull C, Bonhomme S, Chory J, Rameau C, Boyer FD (2016)
Nat Chemical Biology 12 :787