| Title : Synergy between enzymes from Aspergillus involved in the degradation of plant cell wall polysaccharides - de Vries_2000_Carbohydr.Res_327_401 |
| Author(s) : de Vries RP , Kester HC , Poulsen CH , Benen JA , Visser J |
| Ref : Carbohydr Res , 327 :401 , 2000 |
| Abstract : Synergy in the degradation of two plant cell wall polysaccharides, water insoluble pentosan from wheat flour (an arabinoxylan) and sugar beet pectin, was studied using several main-chain cleaving and accessory enzymes. Synergy was observed between most enzymes tested, although not always to the same extent. Degradation of the xylan backbone by endo-xylanase and beta-xylosidase was influenced most strongly by the action of alpha-L-arabinofuranosidase and arabinoxylan arabinofuranohydrolase resulting in a 2.5-fold and twofold increase in release of xylose, respectively. Ferulic acid release by feruloyl esterase A and 4-O-methyl glucuronic acid release by alpha-glucuronidase depended largely on the degradation of the xylan backbone by endo-xylanase but were also influenced by other enzymes. Degradation of the backbone of the pectin hairy regions resulted in a twofold increase in the release of galactose by beta-galactosidase and endo-galactanase but did not significantly influence the arabinose release by arabinofuranosidase and endo-arabinase. Ferulic acid release from sugar beet pectin by feruloyl esterase A was affected most strongly by the presence of other accessory enzymes. |
| ESTHER : de Vries_2000_Carbohydr.Res_327_401 |
| PubMedSearch : de Vries_2000_Carbohydr.Res_327_401 |
| PubMedID: 10990025 |
de Vries RP, Kester HC, Poulsen CH, Benen JA, Visser J (2000)
Synergy between enzymes from Aspergillus involved in the degradation of plant cell wall polysaccharides
Carbohydr Res
327 :401
de Vries RP, Kester HC, Poulsen CH, Benen JA, Visser J (2000)
Carbohydr Res
327 :401