Title : Isolation and characterization of arylacetamide deacetylase in cynomolgus macaques - Uno_2015_J.Vet.Med.Sci_77_721 |
Author(s) : Uno Y , Hosokawa M , Imai T |
Ref : J Vet Med Sci , 77 :721 , 2015 |
Abstract :
Arylacetamide deacetylase (AADAC), a microsomal serine esterase, hydrolyzes drugs, such as flutamide, phenacetin and rifampicin. Because AADAC has not been fully investigated at molecular levels in cynomolgus macaques, the non-human primate species widely used in drug metabolism studies, cynomolgus AADAC cDNA was isolated and characterized. The deduced amino acid sequence, highly homologous (92%) to human AADAC, was more closely clustered with human AADAC than the dog, rat or mouse ortholog in a phylogenetic tree. AADAC was flanked by AADACL2 and SUCNR1 in the cynomolgus and human genomes. Moreover, relatively abundant expression of AADAC mRNA was found in liver and jejunum, the drug-metabolizing organs, in cynomolgus macaques, similar to humans. The results suggest molecular similarities of AADAC between cynomolgus macaques and humans. |
PubMedSearch : Uno_2015_J.Vet.Med.Sci_77_721 |
PubMedID: 25715734 |
Substrate | Rifampicin |
Uno Y, Hosokawa M, Imai T (2015)
Isolation and characterization of arylacetamide deacetylase in cynomolgus macaques
J Vet Med Sci
77 :721
Uno Y, Hosokawa M, Imai T (2015)
J Vet Med Sci
77 :721