Bettazzi F

References (1)

Title : Innovative Biocatalysts as Tools to Detect and Inactivate Nerve Agents - Porzio_2018_Sci.Rep_8_13773
Author(s) : Porzio E , Bettazzi F , Mandrich L , Del Giudice I , Restaino OF , Laschi S , Febbraio F , De Luca V , Borzacchiello MG , Carusone TM , Worek F , Pisanti A , Porcaro P , Schiraldi C , De Rosa M , Palchetti I , Manco G
Ref : Sci Rep , 8 :13773 , 2018
Abstract : Pesticides and warfare nerve agents are frequently organophosphates (OPs) or related compounds. Their acute toxicity highlighted more than ever the need to explore applicable strategies for the sensing, decontamination and/or detoxification of these compounds. Herein, we report the use of two different thermostable enzyme families capable to detect and inactivate OPs. In particular, mutants of carboxylesterase-2 from Alicyclobacillus acidocaldarius and of phosphotriesterase-like lactonases from Sulfolobus solfataricus and Sulfolobus acidocaldarius, have been selected and assembled in an optimized format for the development of an electrochemical biosensor and a decontamination formulation, respectively. The features of the developed tools have been tested in an ad-hoc fabricated chamber, to mimic an alarming situation of exposure to a nerve agent. Choosing ethyl-paraoxon as nerve agent simulant, a limit of detection (LOD) of 0.4 nM, after 5 s of exposure time was obtained. Furthermore, an optimized enzymatic formulation was used for a fast and efficient environmental detoxification (>99%) of the nebulized nerve agent simulants in the air and on surfaces. Crucial, large-scale experiments have been possible thanks to production of grams amounts of pure (>90%) enzymes.
ESTHER : Porzio_2018_Sci.Rep_8_13773
PubMedSearch : Porzio_2018_Sci.Rep_8_13773
PubMedID: 30214052