Porzio_2018_Sci.Rep_8_13773

Reference

Title : Innovative Biocatalysts as Tools to Detect and Inactivate Nerve Agents - Porzio_2018_Sci.Rep_8_13773
Author(s) : Porzio E , Bettazzi F , Mandrich L , Del Giudice I , Restaino OF , Laschi S , Febbraio F , De Luca V , Borzacchiello MG , Carusone TM , Worek F , Pisanti A , Porcaro P , Schiraldi C , De Rosa M , Palchetti I , Manco G
Ref : Sci Rep , 8 :13773 , 2018
Abstract : Pesticides and warfare nerve agents are frequently organophosphates (OPs) or related compounds. Their acute toxicity highlighted more than ever the need to explore applicable strategies for the sensing, decontamination and/or detoxification of these compounds. Herein, we report the use of two different thermostable enzyme families capable to detect and inactivate OPs. In particular, mutants of carboxylesterase-2 from Alicyclobacillus acidocaldarius and of phosphotriesterase-like lactonases from Sulfolobus solfataricus and Sulfolobus acidocaldarius, have been selected and assembled in an optimized format for the development of an electrochemical biosensor and a decontamination formulation, respectively. The features of the developed tools have been tested in an ad-hoc fabricated chamber, to mimic an alarming situation of exposure to a nerve agent. Choosing ethyl-paraoxon as nerve agent simulant, a limit of detection (LOD) of 0.4 nM, after 5 s of exposure time was obtained. Furthermore, an optimized enzymatic formulation was used for a fast and efficient environmental detoxification (>99%) of the nebulized nerve agent simulants in the air and on surfaces. Crucial, large-scale experiments have been possible thanks to production of grams amounts of pure (>90%) enzymes.
ESTHER : Porzio_2018_Sci.Rep_8_13773
PubMedSearch : Porzio_2018_Sci.Rep_8_13773
PubMedID: 30214052

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Porzio E, Bettazzi F, Mandrich L, Del Giudice I, Restaino OF, Laschi S, Febbraio F, De Luca V, Borzacchiello MG, Carusone TM, Worek F, Pisanti A, Porcaro P, Schiraldi C, De Rosa M, Palchetti I, Manco G (2018)
Innovative Biocatalysts as Tools to Detect and Inactivate Nerve Agents
Sci Rep 8 :13773

Porzio E, Bettazzi F, Mandrich L, Del Giudice I, Restaino OF, Laschi S, Febbraio F, De Luca V, Borzacchiello MG, Carusone TM, Worek F, Pisanti A, Porcaro P, Schiraldi C, De Rosa M, Palchetti I, Manco G (2018)
Sci Rep 8 :13773