Fatima I

References (5)

Title : Evaluation of potential inhibitory effects on acetylcholinesterase, pancreatic lipase, and cancer cell lines using raw leaves extracts of three fabaceae species - Fatima_2023_Heliyon_9_e15909
Author(s) : Fatima I , Safdar N , Akhtar W , Munir A , Saqib S , Ayaz A , Bahadur S , Alrefaei AF , Ullah F , Zaman W
Ref : Heliyon , 9 :e15909 , 2023
Abstract : The present study examined the biological potential and phytochemicals of Sophora mollis, Mucuna pruriens, and Indigofera atropurpurea methanolic leaf extracts. In vitro anti-acetylcholinesterase and anti-lipase assays were performed using different concentrations of plant extracts, and the IC(50) values were determined. The cytotoxic potential of the selected plant extracts was assessed against HeLa, PC3, and 3T3 cell lines using an MTT assay. S. mollis leaf extract displayed the highest inhibition percentage (114.60% +/- 19.95 at 1000 microg/mL) for the anti-acetylcholinesterase activity with a prominent IC(50) value of 75.9 microg/mL. The anti-lipase potential was highest with the M. pruriens leaf extract (355.5 microg/mL IC(50)), followed by the S. mollis extract (862.7 microg/mL IC(50)). Among the cell lines tested, the cytotoxic potential of the I. atropurpurea extract (91.1 ppm IC(50)) against the PC3 cell line was promising. High-performance liquid chromatography revealed gallic acid, chlorogenic acid, caffeic acid, vanillic acid, rutin trihydrate, and quercetin dihydrate in varying concentrations in all plant species. The concentration of chlorogenic acid (69.09 ppm) was highest in M. pruriens, and the caffeic acid concentration (45.20 ppm) was higher in S. mollis. This paper reports the presence of bioactive therapeutic compounds in selected species of the Fabaceae family that could be micro-propagated, isolated, and utilized in pharmaceutical industries.
ESTHER : Fatima_2023_Heliyon_9_e15909
PubMedSearch : Fatima_2023_Heliyon_9_e15909
PubMedID: 37206037

Title : Inactivation of tesA reduces cell wall lipid production and increases drug susceptibility in mycobacteria - Chavadi_2011_J.Biol.Chem_286_24616
Author(s) : Chavadi SS , Edupuganti UR , Vergnolle O , Fatima I , Singh SM , Soll CE , Quadri LE
Ref : Journal of Biological Chemistry , 286 :24616 , 2011
Abstract : Phthiocerol dimycocerosates (PDIMs) and phenolic glycolipids (PGLs) are structurally related lipids noncovalently bound to the outer cell wall layer of Mycobacterium tuberculosis, Mycobacterium leprae, and several opportunistic mycobacterial human pathogens. PDIMs and PGLs are important effectors of virulence. Elucidation of the biosynthesis of these complex lipids will not only expand our understanding of mycobacterial cell wall biosynthesis, but it may also illuminate potential routes to novel therapeutics against mycobacterial infections. We report the construction of an in-frame deletion mutant of tesA (encoding a type II thioesterase) in the opportunistic human pathogen Mycobacterium marinum and the characterization of this mutant and its corresponding complemented strain control in terms of PDIM and PGL production. The growth and antibiotic susceptibility of these strains were also probed and compared with the parental wild-type strain. We show that deletion of tesA leads to a mutant that produces only traces of PDIMs and PGLs, has a slight growth yield increase and displays a substantial hypersusceptibility to several antibiotics. We also provide a robust model for the three-dimensional structure of M. marinum TesA (TesAmm) and demonstrate that a Ser-to-Ala substitution in the predicted catalytic Ser of TesAmm renders a mutant that recapitulates the phenotype of the tesA deletion mutant. Overall, our studies demonstrate a critical role for tesA in mycobacterial biology, advance our understanding of the biosynthesis of an important group of polyketide synthase-derived mycobacterial lipids, and suggest that drugs aimed at blocking PDIM and/or PGL production might synergize with antibiotic therapy in the control of mycobacterial infections.
ESTHER : Chavadi_2011_J.Biol.Chem_286_24616
PubMedSearch : Chavadi_2011_J.Biol.Chem_286_24616
PubMedID: 21592957
Gene_locus related to this paper: myctu-yt28 , mycmm-tesa

Title : Butyrylcholinesterase, lipoxygenase inhibiting and antifungal alkaloids from Isatis tinctoria - Ahmad_2008_J.Enzyme.Inhib.Med.Chem_23_313
Author(s) : Ahmad I , Fatima I
Ref : J Enzyme Inhib Med Chem , 23 :313 , 2008
Abstract : Phytochemical investigations on the alkaloidal fraction of the whole plant of the Isatis tinctoria led to the isolation of the alkaloids 1-6. Compounds 3, 2 were found to be potent butyrylcholinesterase and lipoxygenase enzymes inhibitors in a concentration-dependent manner with the IC(50) values 16.3 +/- 0.06 and 19.7 +/- 0.03 microM against BChE and 30.6 +/- 0.02 and 33.7 +/- 0.05 microM against LOX, respectively. The compounds (1-6) showed significant antifungal activity against Trichophyton schoen leinii, Aspergillus niger, Candida albicans, Trichophyton simii, and Macrophomina phaseolina.
ESTHER : Ahmad_2008_J.Enzyme.Inhib.Med.Chem_23_313
PubMedSearch : Ahmad_2008_J.Enzyme.Inhib.Med.Chem_23_313
PubMedID: 18569333

Title : New butyrylcholinesterase inhibitory steroid and peroxy acid from Leucas urticifolia - Fatima_2008_Arch.Pharm.Res_31_999
Author(s) : Fatima I , Ahmad I , Anis I , Malik A , Afza N , Iqbal L , Latif M
Ref : Arch Pharm Res , 31 :999 , 2008
Abstract : A new steroid leucisterol (1) and a new peroxy acid urticic acid (2) along with methoxybenzyl benzoate (3), 4-hydroxy benzoic acid (4), beta-sitosterol (5), and ursolic acid (6), have been isolated from the chloroform soluble fraction of the whole plant of Leucas urticifolia. Their structures were elucidated on the basis of nuclear magnetic resonance (1D and 2D NMR) spectral data. Leucisterol showed potent inhibitory activity against butyrylcholinesterase enzyme.
ESTHER : Fatima_2008_Arch.Pharm.Res_31_999
PubMedSearch : Fatima_2008_Arch.Pharm.Res_31_999
PubMedID: 18787788

Title : Leufolins A and B, potent butyrylcholinesterase-inhibiting flavonoid glucosides from Leucas urticifolia - Atia_2007_Molecules_12_1447
Author(s) : Atia tun N , Fatima I , Ahmad I , Malik A , Afza N , Iqbal L , Latif M , Khan SB
Ref : Molecules , 12 :1447 , 2007
Abstract : New flavonoidal glucosides leufolins A (1) and B (2), have been isolated from the ethyl acetate soluble fraction of the whole plants of Leucas urticifolia. Their structures were elucidated on the basis of extensive analysis of nuclear magnetic resonance (1D and 2D NMR) spectral data. Both of these compounds exhibited significant inhibitory potential against the enzyme butyrylcholinesterase.
ESTHER : Atia_2007_Molecules_12_1447
PubMedSearch : Atia_2007_Molecules_12_1447
PubMedID: 17909500