George R

References (6)

Title : A Drosophila full-length cDNA resource - Stapleton_2002_Genome.Biol_3_RESEARCH0080
Author(s) : Stapleton M , Carlson J , Brokstein P , Yu C , Champe M , George R , Guarin H , Kronmiller B , Pacleb J , Park S , Wan K , Rubin GM , Celniker SE
Ref : Genome Biol , 3 :RESEARCH0080 , 2002
Abstract : BACKGROUND: A collection of sequenced full-length cDNAs is an important resource both for functional genomics studies and for the determination of the intron-exon structure of genes. Providing this resource to the Drosophila melanogaster research community has been a long-term goal of the Berkeley Drosophila Genome Project. We have previously described the Drosophila Gene Collection (DGC), a set of putative full-length cDNAs that was produced by generating and analyzing over 250,000 expressed sequence tags (ESTs) derived from a variety of tissues and developmental stages.
RESULTS: We have generated high-quality full-insert sequence for 8,921 clones in the DGC. We compared the sequence of these clones to the annotated Release 3 genomic sequence, and identified more than 5,300 cDNAs that contain a complete and accurate protein-coding sequence. This corresponds to at least one splice form for 40% of the predicted D. melanogaster genes. We also identified potential new cases of RNA editing.
CONCLUSIONS: We show that comparison of cDNA sequences to a high-quality annotated genomic sequence is an effective approach to identifying and eliminating defective clones from a cDNA collection and ensure its utility for experimentation. Clones were eliminated either because they carry single nucleotide discrepancies, which most probably result from reverse transcriptase errors, or because they are truncated and contain only part of the protein-coding sequence.
ESTHER : Stapleton_2002_Genome.Biol_3_RESEARCH0080
PubMedSearch : Stapleton_2002_Genome.Biol_3_RESEARCH0080
PubMedID: 12537569
Gene_locus related to this paper: drome-KRAKEN

Title : An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region - Ashburner_1999_Genetics_153_179
Author(s) : Ashburner M , Misra S , Roote J , Lewis SE , Blazej R , Davis T , Doyle C , Galle R , George R , Harris N , Hartzell G , Harvey D , Hong L , Houston K , Hoskins R , Johnson G , Martin C , Moshrefi A , Palazzolo M , Reese MG , Spradling A , Tsang G , Wan K , Whitelaw K , Celniker S , Rubin GM
Ref : Genetics , 153 :179 , 1999
Abstract : A contiguous sequence of nearly 3 Mb from the genome of Drosophila melanogaster has been sequenced from a series of overlapping P1 and BAC clones. This region covers 69 chromosome polytene bands on chromosome arm 2L, including the genetically well-characterized "Adh region." A computational analysis of the sequence predicts 218 protein-coding genes, 11 tRNAs, and 17 transposable element sequences. At least 38 of the protein-coding genes are arranged in clusters of from 2 to 6 closely related genes, suggesting extensive tandem duplication. The gene density is one protein-coding gene every 13 kb; the transposable element density is one element every 171 kb. Of 73 genes in this region identified by genetic analysis, 49 have been located on the sequence; P-element insertions have been mapped to 43 genes. Ninety-five (44%) of the known and predicted genes match a Drosophila EST, and 144 (66%) have clear similarities to proteins in other organisms. Genes known to have mutant phenotypes are more likely to be represented in cDNA libraries, and far more likely to have products similar to proteins of other organisms, than are genes with no known mutant phenotype. Over 650 chromosome aberration breakpoints map to this chromosome region, and their nonrandom distribution on the genetic map reflects variation in gene spacing on the DNA. This is the first large-scale analysis of the genome of D. melanogaster at the sequence level. In addition to the direct results obtained, this analysis has allowed us to develop and test methods that will be needed to interpret the complete sequence of the genome of this species. Before beginning a Hunt, it is wise to ask someone what you are looking for before you begin looking for it. Milne 1926
ESTHER : Ashburner_1999_Genetics_153_179
PubMedSearch : Ashburner_1999_Genetics_153_179
PubMedID: 10471707

Title : A model hypocholinergic syndrome produced by a false choline analog, N-aminodeanol - Jenden_1987_J.Neural.Transm.Suppl_24_325
Author(s) : Jenden DJ , Russell RW , Booth RA , Lauretz SD , Knusel BJ , Roch M , Rice KM , George R , Waite JJ
Ref : J Neural Transm Suppl , 24 :325 , 1987
Abstract : N-aminodeanol is an analog of choline that serves as a less effective substrate in all of its known enzymatic and transport mechanisms. It was utilized to test the hypothesis that the selective vulnerability of cholinergic neurones in Alzheimer's disease is due to competition for the available choline between pathways for acetylcholine and phospholipid synthesis. Rats placed on a choline free diet containing an equivalent amount of N-aminodeanol develop a model hypocholinergic state comprising hyperreactivity, hyperalgesia, aggressive behavior and a deficit in learning and memory. These effects are associated with a progressive replacement of free and lipid-bound choline and acetylcholine with N-aminodeanol and its corresponding esters. Choline acetyltransferase is reduced in some brain regions, suggesting a loss of cholinergic neurones. We propose that this represents a potentially useful animal model of Alzheimer's disease which deserves further investigation.
ESTHER : Jenden_1987_J.Neural.Transm.Suppl_24_325
PubMedSearch : Jenden_1987_J.Neural.Transm.Suppl_24_325
PubMedID: 3479528

Title : Acetylene compounds of potential pharmacological value. XII. Central and peripheral anticholinergic activity of t-aminoalkynyl esters of some carboxylic acids -
Author(s) : Dahlbom R , Erbing B , Olsson K , George R , Jenden DJ
Ref : Acta Pharm Suec , 6 :349 , 1969
PubMedID: 5824849

Title : Acetylene compounds of potential pharmacological value. IX. N-(4-dialkylamino-2-butynyl)-substituted carboxylic and sulphonic acid amides -
Author(s) : Dahlbom R , Karlen B , Lindquist A , George R , Jenden DJ
Ref : Acta Pharm Suec , 4 :247 , 1967
PubMedID: 6082975

Title : Acetylene compounds of potential pharmacological value. 8. N-(4-dialkylamino-2-butynyl)-substituted cyclic imides -
Author(s) : Dahlbom R , Karlen B , George R , Jenden DJ
Ref : Journal of Medicinal Chemistry , 9 :843 , 1966
PubMedID: 5972043