Misra S

References (13)

Title : Role of Genetic Variants and Gene Expression in the Susceptibility and Severity of COVID-19 - Choudhary_2021_Ann.Lab.Med_41_129
Author(s) : Choudhary S , Sreenivasulu K , Mitra P , Misra S , Sharma P
Ref : Ann Lab Med , 41 :129 , 2021
Abstract : Since its first report in December 2019, coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly emerged as a pandemic affecting nearly all countries worldwide. As the COVID-19 pandemic progresses, the need to identify genetic risk factors for susceptibility to this serious illness has emerged. Host genetic factors, along with other risk factors may help determine susceptibility to respiratory tract infections. It is hypothesized that the ACE2 gene, encoding angiotensin-converting enzyme 2 (ACE2), is a genetic risk factor for SARS-CoV-2 infection and is required by the virus to enter cells. Together with ACE2, transmembrane protease serine 2 (TMPRSS2) and dipeptidyl peptidase-4 (DPP4) also play an important role in disease severity. Evaluating the role of genetic variants in determining the direction of respiratory infections will help identify potential drug target candidates for further study in COVID-19 patients. We have summarized the latest reports demonstrating that ACE2 variants, their expression, and epigenetic factors may influence an individual's susceptibility to SARS-CoV-2 infection and disease outcome.
ESTHER : Choudhary_2021_Ann.Lab.Med_41_129
PubMedSearch : Choudhary_2021_Ann.Lab.Med_41_129
PubMedID: 33063674

Title : Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest - Rajamanikyam_2017_EXCLI.J_16_375
Author(s) : Rajamanikyam M , Vadlapudi V , Parvathaneni SP , Koude D , Sripadi P , Misra S , Amanchy R , Upadhyayula SM
Ref : EXCLI J , 16 :375 , 2017
Abstract : Bacteria belonging to the family Brevibacterieae are ubiquitous Gram positive organisms that are responsible for the feet odour and cheese aroma. Brevibacterium mcbrellneri is a relatively new member belonging to Brevibacterieae. In the current manuscript we discuss isolation of biologically active metabolites from Brevibacterium mcbrellneri. Two aromatic esters were isolated from Brevibacterium mcbrellneri by "Bioassay guided fractionation strategy" and identified as di-(2-ethylhexyl) phthalate and dibutyl phthalate by chemical characterization using biophysical techniques. The phthalate compounds show broad spectrum antibacterial activity and mosquito larvicidal activity. Mosquito larvicidal activity has been attributed to inhibition of acetylcholinesterase enzyme activity. These compounds were found to be cytotoxic in multiple cell lines causing cell cycle arrest in G1 phase.
ESTHER : Rajamanikyam_2017_EXCLI.J_16_375
PubMedSearch : Rajamanikyam_2017_EXCLI.J_16_375
PubMedID: 28507481

Title : Neuroprotective potential of sesamol and its loaded solid lipid nanoparticles in ICV-STZ-induced cognitive deficits: Behavioral and biochemical evidence - Sachdeva_2015_Eur.J.Pharmacol_747_132
Author(s) : Sachdeva AK , Misra S , Pal Kaur I , Chopra K
Ref : European Journal of Pharmacology , 747 :132 , 2015
Abstract : Neuroinflammation is a prominent feature of Alzheimer disease (AD) and other chronic neurodegenerative disorders. Intracerebroventricular (ICV) streptozotocin (STZ) induced-cognitive impairment has been widely used as an experimental paradigm of Alzheimers disease. Sesamol is a potent inhibitor of cytokine production as well as an antioxidant. The present study was designed to evaluate the effectiveness of sesamol in ICV-STZ-induced cognitive deficits in rats by incorporating it into solid lipid nanoparticles (SLNs). ICV-STZ administration produced significant cognitive deficits as assessed by both Morris water maze and elevated plus maze task which is accompanied by significantly enhanced nitrodative stress, altered acetylcholinesterase in rat brain along with significantly increased serum TNF-alpha levels. Chronic treatment with sesamol and sesamol loaded SLNs dose dependently restored cognitive deficits in ICV-STZ rats along with mitigation of nitrodative stress and cytokine release. Effectiveness of SLNs to deliver sesamol to the brain was shown by a significantly better alleviation of the oxidative stress parameters. Our findings demonstrate that loading of sesamol in SLNs is an effective strategy to mitigate ICV-STZ-induced neuronal dysfunction and memory deficits.
ESTHER : Sachdeva_2015_Eur.J.Pharmacol_747_132
PubMedSearch : Sachdeva_2015_Eur.J.Pharmacol_747_132
PubMedID: 25449035

Title : Galantamine-loaded solid-lipid nanoparticles for enhanced brain delivery: preparation, characterization, in vitro and in vivo evaluations - Misra_2015_Drug.Deliv__1
Author(s) : Misra S , Chopra K , Sinha VR , Medhi B
Ref : Drug Deliv , :1 , 2015
Abstract : Galantamine hydrobromide, a promising acetylcholinesterase inhibitor is reported to be associated with cholinergic side effects. Its poor brain penetration results in lower bioavailability to the target site. With an aim to overcome these limitations, solid-lipid nanoparticulate formulation of galantamine hydrobromide was developed employing biodegradable and biocompatible components. The selected galantamine hydrobromide-loaded solid-lipid nanoparticles offered nanocolloidal with size lower than 100 nm and maximum drug entrapment 83.42 +/- 0.63%. In vitro drug release from these spherical drug-loaded nanoparticles was observed to be greater than 90% for a period of 24 h in controlled manner. In vivo evaluations demonstrated significant memory restoration capability in cognitive deficit rats in comparison with naive drug. The developed carriers offered approximately twice bioavailability to that of plain drug. Hence, the galantamine hydrobromide-loaded solid-lipid nanoparticles can be a promising vehicle for safe and effective delivery especially in disease like Alzheimer's.
ESTHER : Misra_2015_Drug.Deliv__1
PubMedSearch : Misra_2015_Drug.Deliv__1
PubMedID: 26405825

Title : Draft genome of Ochrobactrum intermedium strain M86 isolated from non-ulcer dyspeptic individual from India - Kulkarni_2013_Gut.Pathog_5_7
Author(s) : Kulkarni G , Dhotre D , Dharne M , Shetty S , Chowdhury S , Misra V , Misra S , Patole M , Shouche Y
Ref : Gut Pathog , 5 :7 , 2013
Abstract : BACKGROUND: Ochrobactrum intermedium is an emerging opportunistic pathogen of humans that is closely related to members of the genus Brucella. Earlier, we reported the case of an Indian subject with non-ulcer dyspeptic symptoms whose urease positive gastric biopsy revealed the presence of Helicobacter pylori along with non-Helicobacter like bacteria, eventually cultured and identified as O. intermedium strain M86.
RESULTS: Here, we describe the unclosed draft genome of the strain M86 with a length of 5,188,688 bp and mean G+C content of 57.9%. We have also identified many putative gene clusters that might be responsible for its persistence in the gastric mucosa.Comparative analysis of genomic features of Ochrobactrum intermedium strain M86 and Ochrobactrum intermedium LMG 3301T was also done.
CONCLUSIONS: This paper attempts to gain whole-genome based insights into the putative gene determinants of O. intermedium for survival in the highly acidic stomach lumen environment .Identification of genes putatively involved in the various metabolic pathways may lead to a better understanding of the survival of O. intermdedium in acidic condition.
ESTHER : Kulkarni_2013_Gut.Pathog_5_7
PubMedSearch : Kulkarni_2013_Gut.Pathog_5_7
PubMedID: 23557353
Gene_locus related to this paper: 9rhiz-c4wlu2

Title : Modulation of nitrergic pathway by sesamol prevents cognitive deficits and associated biochemical alterations in intracerebroventricular streptozotocin administered rats - Misra_2011_Eur.J.Pharmacol_659_177
Author(s) : Misra S , Tiwari V , Kuhad A , Chopra K
Ref : European Journal of Pharmacology , 659 :177 , 2011
Abstract : Alzheimer's disease is a neurodegenerative disorder characterized by progressive cognitive decline and widespread loss of neurons and their synapses in the cerebral cortex and hippocampus. Increasing evidence indicates that factors such as oxidative-nitrergic stress, glutathione depletion, impaired protein metabolism and cholinergic deficit can interact in a vicious cycle, which is central to Alzheimer's disease pathogenesis. Intracerebroventricular (i.c.v.) streptozotocin induced-cognitive impairment has been widely used as an experimental paradigm to study Alzheimer's disease. In the present study, i.c.v. streptozotocin produced significant cognitive deficits as measured in Morris water maze and elevated plus maze task coupled with increased serum TNF-alpha levels and marked rise in brain acetylcholinesterase and oxidative-nitrergic stress in female Wistar rats. Sesamol (5-hydroxy-1,3-benzodioxole or 3,4-methylenedioxyphenol), a potent anti-oxidant and anti-inflammatory molecule markedly improved cognitive impairment, reduced acetylcholinesterase activity, TNF-alpha levels and attenuated oxidative-nitrergic stress in brain of i.c.v.-streptozotocin treated rats. Administration of L-arginine (125 mg/kg i.p), a nitric oxide donor, alone to i.c.v.-streptozotocin treated rats accentuated behavioral and biochemical deficits and also abolished the protective effect of sesamol (8 mg/kg). L-NAME (10 mg/kgi.p.), a non-specific NOS inhibitor significantly restored all the behavioral and biochemical indices in i.c.v.-streptozotocin rats. Moreover, combination of L-NAME with sub-effective dose of sesamol (4 mg/kg) potentiated its protective effect. Our findings demonstrate the effectiveness of sesamol in preventing intracerebroventricular streptozotocin-induced cognitive deficits by modulating nitrergic signaling and oxido-inflammatory cascade.
ESTHER : Misra_2011_Eur.J.Pharmacol_659_177
PubMedSearch : Misra_2011_Eur.J.Pharmacol_659_177
PubMedID: 21463622

Title : Characterization of cross-linked immobilized lipase from thermophilic mould Thermomyces lanuginosa using glutaraldehyde - Gupta_2009_Bioresour.Technol_100_4074
Author(s) : Gupta P , Dutt K , Misra S , Raghuwanshi S , Saxena RK
Ref : Bioresour Technol , 100 :4074 , 2009
Abstract : Cross-linked enzyme aggregates (CLEAs) have emerged as an interesting biocatalyst design for immobilization. Using this approach, a 1,3 regiospecific, alkaline and thermostable lipase from Thermomyces lanuginosa was immobilized. Efficient cross-linking was observed when ammonium sulphate was used as precipitant along with a two fold increase in activity in presence of SDS. The TEM and SEM microphotographs of the CLEAs formed reveal that the enzyme aggregates are larger in size as compared to the free lipase due to the cross-linking of enzyme aggregates with glutaraldehyde. The stability and reusability of the CLEA with respect to olive oil hydrolysis was evaluated. The CLEA showed more than 90% residual activity even after 10 cycles of repeated use.
ESTHER : Gupta_2009_Bioresour.Technol_100_4074
PubMedSearch : Gupta_2009_Bioresour.Technol_100_4074
PubMedID: 19403306

Title : Annotation of the Drosophila melanogaster euchromatic genome: a systematic review - Misra_2002_Genome.Biol_3_RESEARCH0083
Author(s) : Misra S , Crosby MA , Mungall CJ , Matthews BB , Campbell KS , Hradecky P , Huang Y , Kaminker JS , Millburn GH , Prochnik SE , Smith CD , Tupy JL , Whitfied EJ , Bayraktaroglu L , Berman BP , Bettencourt BR , Celniker SE , de Grey AD , Drysdale RA , Harris NL , Richter J , Russo S , Schroeder AJ , Shu SQ , Stapleton M , Yamada C , Ashburner M , Gelbart WM , Rubin GM , Lewis SE
Ref : Genome Biol , 3 :RESEARCH0083 , 2002
Abstract : BACKGROUND: The recent completion of the Drosophila melanogaster genomic sequence to high quality and the availability of a greatly expanded set of Drosophila cDNA sequences, aligning to 78% of the predicted euchromatic genes, afforded FlyBase the opportunity to significantly improve genomic annotations. We made the annotation process more rigorous by inspecting each gene visually, utilizing a comprehensive set of curation rules, requiring traceable evidence for each gene model, and comparing each predicted peptide to SWISS-PROT and TrEMBL sequences.
RESULTS: Although the number of predicted protein-coding genes in Drosophila remains essentially unchanged, the revised annotation significantly improves gene models, resulting in structural changes to 85% of the transcripts and 45% of the predicted proteins. We annotated transposable elements and non-protein-coding RNAs as new features, and extended the annotation of untranslated (UTR) sequences and alternative transcripts to include more than 70% and 20% of genes, respectively. Finally, cDNA sequence provided evidence for dicistronic transcripts, neighboring genes with overlapping UTRs on the same DNA sequence strand, alternatively spliced genes that encode distinct, non-overlapping peptides, and numerous nested genes.
CONCLUSIONS: Identification of so many unusual gene models not only suggests that some mechanisms for gene regulation are more prevalent than previously believed, but also underscores the complex challenges of eukaryotic gene prediction. At present, experimental data and human curation remain essential to generate high-quality genome annotations.
ESTHER : Misra_2002_Genome.Biol_3_RESEARCH0083
PubMedSearch : Misra_2002_Genome.Biol_3_RESEARCH0083
PubMedID: 12537572
Gene_locus related to this paper: drome-a1z6g9 , drome-abhd2 , drome-ACHE , drome-CG8058 , drome-CG8093 , drome-CG8233 , drome-CG8425 , drome-CG9059 , drome-CG9186 , drome-CG9542 , drome-CG10982 , drome-CG11309 , drome-CG11406 , drome-CG11598 , drome-CG17097 , drome-glita , drome-KRAKEN , drome-nrtac , drome-OME , drome-q7k274 , drome-q9vux3

Title : An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region - Ashburner_1999_Genetics_153_179
Author(s) : Ashburner M , Misra S , Roote J , Lewis SE , Blazej R , Davis T , Doyle C , Galle R , George R , Harris N , Hartzell G , Harvey D , Hong L , Houston K , Hoskins R , Johnson G , Martin C , Moshrefi A , Palazzolo M , Reese MG , Spradling A , Tsang G , Wan K , Whitelaw K , Celniker S , Rubin GM
Ref : Genetics , 153 :179 , 1999
Abstract : A contiguous sequence of nearly 3 Mb from the genome of Drosophila melanogaster has been sequenced from a series of overlapping P1 and BAC clones. This region covers 69 chromosome polytene bands on chromosome arm 2L, including the genetically well-characterized "Adh region." A computational analysis of the sequence predicts 218 protein-coding genes, 11 tRNAs, and 17 transposable element sequences. At least 38 of the protein-coding genes are arranged in clusters of from 2 to 6 closely related genes, suggesting extensive tandem duplication. The gene density is one protein-coding gene every 13 kb; the transposable element density is one element every 171 kb. Of 73 genes in this region identified by genetic analysis, 49 have been located on the sequence; P-element insertions have been mapped to 43 genes. Ninety-five (44%) of the known and predicted genes match a Drosophila EST, and 144 (66%) have clear similarities to proteins in other organisms. Genes known to have mutant phenotypes are more likely to be represented in cDNA libraries, and far more likely to have products similar to proteins of other organisms, than are genes with no known mutant phenotype. Over 650 chromosome aberration breakpoints map to this chromosome region, and their nonrandom distribution on the genetic map reflects variation in gene spacing on the DNA. This is the first large-scale analysis of the genome of D. melanogaster at the sequence level. In addition to the direct results obtained, this analysis has allowed us to develop and test methods that will be needed to interpret the complete sequence of the genome of this species. Before beginning a Hunt, it is wise to ask someone what you are looking for before you begin looking for it. Milne 1926
ESTHER : Ashburner_1999_Genetics_153_179
PubMedSearch : Ashburner_1999_Genetics_153_179
PubMedID: 10471707

Title : Secretory acetylcholinesterase of Setaria cervi microfilariae and its antigenic cross-reactivity with Wuchereria bancrofti - Sharma_1998_Trop.Med.Int.Health_3_46
Author(s) : Sharma S , Misra S , Rathaur S
Ref : Trop Med Int Health , 3 :46 , 1998
Abstract : Setaria cervi, a bovine filarial parasite, secretes acetylcholinesterase during in vitro cultivation. A significant amount of enzyme activity was detected both in culture media and somatic extracts of different developmental stages of the parasite. The microfilarial stage showed a higher level of AChE activity than adult worms, with females being considerably more active than males. The secretory enzyme from microfilariae preferentially utilized acetylthiocholine iodide as substrate and showed two electrophoretically distinct isoforms in native PAGE. Secretory enzyme was purified from the excretory/secretory products of microfilariae using edrophonium chloride linked to epoxy-activated sepharose. Analysis of purified acetylcholinesterase by SDS-PAGE revealed the existence of two proteins of 75kD and 45kD under nonreducing conditions. These secretory enzymes are antigenic and cross-reactive with Wuchereria bancrofti-infected asymptomatic microfilaraemic human sera when tested by enzyme linked immunosorbent assay and immunoblotting. The secretory AChE(s) from S. cervi microfilariae may be utilized for diagnosis of early filarial infections.
ESTHER : Sharma_1998_Trop.Med.Int.Health_3_46
PubMedSearch : Sharma_1998_Trop.Med.Int.Health_3_46
PubMedID: 9484968

Title : Diethylcarbamazine: effect on lysosomal enzymes and acetylcholine in Wuchereria bancrofti infection - Bhattacharya_1997_Trop.Med.Int.Health_2_686
Author(s) : Bhattacharya C , Singh RN , Misra S , Rathaur S
Ref : Trop Med Int Health , 2 :686 , 1997
Abstract : We measured the levels of lysosomal enzymes and acetylcholine in Wuchereria bancrofti-infected asymptomatic microfilaraemic human serum, and found a significant decrease in the activity of beta-glucuronidase and acid phosphatase compared to normal serum. Acetylcholine levels were also decreased during infection. However, after giving diethylcarbamazine (6 mg/kg body wt/day) the level of lysosomal enzymes and acetylcholine increased and reached a normal value after two weeks of therapy. It is proposed that parasites secrete acetylcholinesterase in the circulation which degrades acetylcholine. Since acetylcholine stimulates the release of lysosomal enzymes and phagocytosis, the immune response of the host is suppressed during infection. During diethylcarbamazine (DEC) therapy the parasitic enzyme is inhibited by the drug and the normal level of acetylcholine is resumed, which again stimulates the release of lysosomal enzyme and the process of phagocytosis.
ESTHER : Bhattacharya_1997_Trop.Med.Int.Health_2_686
PubMedSearch : Bhattacharya_1997_Trop.Med.Int.Health_2_686
PubMedID: 9270736

Title : Wuchereria bancrofti: identification of parasitic acetylcholinesterase in microfilariae infected human serum - Misra_1993_Trop.Med.Parasitol_44_75
Author(s) : Misra S , Mohapatra TM , Rathaur S
Ref : Trop Med Parasitol , 44 :75 , 1993
Abstract : An antigen with cholinesterase activity was detected in the sera of patients infected with Wuchereria bancrofti. The asymptomatic microfilaremic sera showed 3 to 4 times more cholinesterase activity for acetylthiocholine (ATCh) as compared to sera of symptomatic amicrofilaremic, hookworm infected and endemic normals, whereas the activities for butyrylthiocholine (BTCh) did not significantly differ. The enzyme activities from both sources, namely from sera of microfilaremic cases and from endemic normals, were partially purified and according to substrate specificity for ATCh and BTCh as well as inhibition of the former activity by excess substrate classified as acetylcholinesterase (AChE; EC and pseudocholinesterase (AChE; EC, respectively. The Km-value for ATCh of the cholinesterase from the microfilaremic sera was determined to be 0.87 mM. Eserine competitively inhibited the AChE activity; the inhibition constant was found to be 1.3 microM. The BChE from the normal sera had Km-values of 0.15 and 0.20 mM for BTCh and ATCh, respectively, and did not show significant inhibition by eserine. These and other dissimilarities suggest a difference in nature of the cholinesterases in microfilaremic and normal sera and propose that the former enzyme, a true acetylcholinesterase, originates from the parasite. Additional evidence for the origin of the AChE-activity from the parasite was provided by ELISA-studies; anti-Brugia malayi AChE antibodies confirmed antigenecity and cross reactivity of the AChE in infected sera, whereas the antibodies did not show any cross reactivity with the BChE in normal sera.
ESTHER : Misra_1993_Trop.Med.Parasitol_44_75
PubMedSearch : Misra_1993_Trop.Med.Parasitol_44_75
PubMedID: 8367669

Title : In vitro and in vivo effect of diethylcarbamazine on the activity of acetylcholinesterase from Wuchereria bancrofti infected human serum - Misra_1992_Trop.Med.Parasitol_43_95
Author(s) : Misra S , Taneja V , Rathaur S
Ref : Trop Med Parasitol , 43 :95 , 1992
Abstract : The acetylcholinesterase (AChE) activity was measured in human serum from persons infected with the filarial parasite Wuchereria bancrofti. The asymptomatic microfilaremic serum showed five times increase in AChE-activity as compared with normal serum, whereas only little difference was observed in serum from patients with elephantiasis. Similar results were obtained when the enzyme activity was measured in the immune complexes precipitated with polyethyleneglycol. Further, the effect of the antifilarial drug diethylcarbamazine (DEC), on the AChE activity of infected and normal serum was studied in in vivo and in vitro experiments. In vitro, DEC was found to be effective only with respect to AChE from asymptomatic microfilaremic serum where 75% decrease in enzyme activity was observed at 100 mumol. The oral administration of DEC (5 mg/kg of body weight/day) effected the activity of AChE from microfilaremic serum as shown after 1 hr, 1 and 3 weeks. A regular decrease in enzyme activity of asymptomatic microfilaremic serum was observed. By increasing time periods and after three weeks the level of AChE reaches the normal value. In vitro and in vivo the same concentration of DEC has negligible effect on the normal serum suggesting that in case of asymptomatic microfilaremic serum the increased activity of AChE is different in nature than the host acetylcholinesterase
ESTHER : Misra_1992_Trop.Med.Parasitol_43_95
PubMedSearch : Misra_1992_Trop.Med.Parasitol_43_95