Hashimoto Y

References (19)

Title : The Impact of Nutritional Markers and Dietary Habits on the Bioimpedance Phase Angle in Older Individuals - Kajiyama_2023_Nutrients_15_
Author(s) : Kajiyama S , Nakanishi N , Yamamoto S , Ichikawa T , Okamura T , Hashimoto Y , Kitagawa N , Hamaguchi M , Fukui M
Ref : Nutrients , 15 : , 2023
Abstract : Low phase angle (PhA), as determined via bioelectrical impedance analysis, reflects unhealthy aging and mortality. In this study, we assessed whether nutritional status, including serum nutritional markers and dietary habits, is related to PhA in older individuals. We recruited 212 participants (aged <= 65 years) who underwent medical health checkups. PhA was measured using a multi-frequency impedance body composition analyzer. Habitual food and nutrient intake was evaluated using a brief, self-administered diet history questionnaire. Low PhA values were defined as >=4.95 in males and >=4.35 in females. Males with low PhA had poor exercise habits (p = 0.0429) and a lower body mass index (p = 0.0024). PhA was significantly correlated with serum cholinesterase levels, a nutritional status marker (r = 0.3313, p = 0.0004 in males; r = 0.3221, p = 0.0070 in females). The low-PhA group had significantly lower total energy and carbohydrate intake per ideal body weight (IBW) than the high-PhA group in males (total energy intake:30.2 +/- 9.8 and 34.5 +/- 9.3 kcal/kg/day, p = 0.0307; carbohydrate intake:15.2 +/- 4.9 and 18.0 +/- 5.8 kcal/kg/day, p = 0.0157). Total energy intake per IBW (adjusted odds ratio [95% confidence interval], 0.94 [0.89-1.00] per 1 kcal/kg/day increase) was independently associated with a low PhA in males. Our study revealed that lower total energy intake independently impacted low PhA in older males.
ESTHER : Kajiyama_2023_Nutrients_15_
PubMedSearch : Kajiyama_2023_Nutrients_15_
PubMedID: 37630789

Title : Congenital hyperinsulinism associated with Hirschsprung's disease-a report of an extremely rare case - Shono_2020_Surg.Case.Rep_6_4
Author(s) : Shono T , Shono K , Hashimoto Y , Taguchi S , Masuda M , Muramori K , Taguchi T
Ref : Surg Case Rep , 6 :4 , 2020
Abstract : BACKGROUND: Congenital hyperinsulinism (CH) is a rare disease, characterized by severe hypoglycemia induced by inappropriate insulin secretion from pancreatic beta-cells in neonate and infant. Hirschsprung's disease (HD) is also a rare disease in which infants show severe bowel movement disorder. We herein report an extremely rare case of combined CH and HD. CASE PRESENTATION: The patient was a full-term male infant who showed poor feeding, vomiting, and hypotonia with lethargy on the day of birth. He was transferred to tertiary hospital after a laboratory analysis revealed hyperinsulinemic hypoglycemia. The patient showed remarkable abdominal distension without meconium defecation. An abdominal X-ray showed marked dilatation of the large bowel. He was diagnosed with CH (nesidioblastosis) associated with suspected HD. He was initially treated with an intravenous infusion of high-dose glucose with the intermittent injection of glucagon. This was successfully followed by treatment with diazoxide and octreotide (a somatostatin analog). At 8 months of age, HD was confirmed by the acetylcholinesterase staining of a rectal mucosal biopsy specimen, and a transanal pull-through operation was performed to treat HD. At 14 months of age, subtotal pancreatectomy was performed for the treatment of focal CH located in the pancreatic body. His postoperative course over the past 12 years has been uneventful without any neurologic or bowel movement disorders. CONCLUSIONS: Although it is extremely rare for CH to be associated with HD, associated HD should be considered when a patient with CH presents severe constipation.
ESTHER : Shono_2020_Surg.Case.Rep_6_4
PubMedSearch : Shono_2020_Surg.Case.Rep_6_4
PubMedID: 31916119

Title : Glioblastoma stem cell-derived exosomes induce M2 macrophages and PD-L1 expression on human monocytes - Gabrusiewicz_2018_Oncoimmunology_7_e1412909
Author(s) : Gabrusiewicz K , Li X , Wei J , Hashimoto Y , Marisetty AL , Ott M , Wang F , Hawke D , Yu J , Healy LM , Hossain A , Akers JC , Maiti SN , Yamashita S , Shimizu Y , Dunner K , Zal MA , Burks JK , Gumin J , Nwajei F , Rezavanian A , Zhou S , Rao G , Sawaya R , Fuller GN , Huse JT , Antel JP , Li S , Cooper L , Sulman EP , Chen C , Geula C , Kalluri R , Zal T , Heimberger AB
Ref : Oncoimmunology , 7 :e1412909 , 2018
Abstract : Exosomes can mediate a dynamic method of communication between malignancies, including those sequestered in the central nervous system and the immune system. We sought to determine whether exosomes from glioblastoma (GBM)-derived stem cells (GSCs) can induce immunosuppression. We report that GSC-derived exosomes (GDEs) have a predilection for monocytes, the precursor to macrophages. The GDEs traverse the monocyte cytoplasm, cause a reorganization of the actin cytoskeleton, and skew monocytes toward the immune suppresive M2 phenotype, including programmed death-ligand 1 (PD-L1) expression. Mass spectrometry analysis demonstrated that the GDEs contain a variety of components, including members of the signal transducer and activator of transcription 3 (STAT3) pathway that functionally mediate this immune suppressive switch. Western blot analysis revealed that upregulation of PD-L1 in GSC exosome-treated monocytes and GBM-patient-infiltrating CD14(+) cells predominantly correlates with increased phosphorylation of STAT3, and in some cases, with phosphorylated p70S6 kinase and Erk1/2. Cumulatively, these data indicate that GDEs are secreted GBM-released factors that are potent modulators of the GBM-associated immunosuppressive microenvironment.
ESTHER : Gabrusiewicz_2018_Oncoimmunology_7_e1412909
PubMedSearch : Gabrusiewicz_2018_Oncoimmunology_7_e1412909
PubMedID: 29632728

Title : Significance of preoperative butyrylcholinesterase level as an independent predictor of survival in patients with upper urinary tract urothelial carcinoma treated with nephroureterectomy - Noro_2017_Jpn.J.Clin.Oncol__1
Author(s) : Noro D , Koie T , Hashimoto Y , Tanaka T , Ohyama C , Tobisawa Y , Yoneyama T , Imai A , Hatakeyama S , Yamamoto H , Kitayama M , Hirota K
Ref : Japanese Journal of Clinical Oncology , :1 , 2017
Abstract : Objectives: Butyrylcholinesterase (BChE) is an alpha-glycoprotein synthesized in the liver. Its serum levels are reportedly correlated with disease activity in patients with cancer. The aim of this study was to estimate the potential prognostic significance of preoperative serum BChE levels in patients with upper urinary tract urothelial carcinoma (UTUC) undergoing radical nephroureterectomy (RNU). Methods: Of the 220 patients with UTUC who underwent RNU between 1995 and 2016 at Hirosaki University Hospital, 149 patients with available laboratory data were included for analysis. Covariates included age, sex, preoperative laboratory data, clinical T and N grades, tumor grade, tumor location and preoperative chemotherapy. Univariate and multivariate analyses were performed to identify clinical factors associated with overall survival (OS) and disease-free survival (DFS). Univariate analysis was performed using the Kaplan-Meier and log-rank methods, and the multivariate analysis was performed using a Cox proportional hazard model. Results: The median BChE level was 276 U/l and the optimal cut-off point for the serum BChE level was determined to be 218 IU/ml. The 5-year OS and DFS rates were 81.0% and 73.7%, respectively. The 5-year OS and DFS rates were significantly greater in the BChE >/= 218 than <218 U/l groups (86.6% vs. 53.7%, P < 0.001 and 76.4% vs. 58.3%, P = 0.049, respectively). In multivariate analysis, BChE levels were most significantly associated with OS, whereas BChE level and tumor grade were significantly associated with DFS. Conclusions: This study validated preoperative serum BChE levels as an independent prognostic factor for UTUC after RNU.
ESTHER : Noro_2017_Jpn.J.Clin.Oncol__1
PubMedSearch : Noro_2017_Jpn.J.Clin.Oncol__1
PubMedID: 29177431

Title : Significance of preoperative butyrylcholinesterase as an independent predictor of biochemical recurrence-free survival in patients with prostate cancer treated with radical prostatectomy - Koie_2016_Int.J.Clin.Oncol_21_379
Author(s) : Koie T , Ohyama C , Hatakeyama S , Imai A , Yoneyama T , Hashimoto Y , Tobisawa Y , Hosogoe S , Yamamoto H , Kitayama M , Hirota K
Ref : Int J Clin Oncol , 21 :379 , 2016
Abstract : BACKGROUND: Butyrylcholinesterase (BChE) is an alpha-glycoprotein found in the nervous system and liver. Its serum level is reduced in many clinical conditions, such as liver damage, inflammation, injury, infection, malnutrition, and malignant disease. In this study, we analyzed the potential prognostic significance of preoperative BChE levels in patients with prostate cancer (PCa) undergoing radical prostatectomy (RP).
METHODS: We retrospectively evaluated 535 patients with PCa who underwent RP from 1996-2014 at a single institution. Serum BChE was routinely measured in all patients before operation. Covariates included age, preoperative laboratory data [prostate-specific antigen (PSA), hemoglobin, total protein, albumin, BChE, lactate dehydrogenase, C-reactive protein], clinical T, biopsy Gleason score, D'Amico risk classification, and RP with/without neoadjuvant therapy. Univariate and multivariate analyses were performed to identify clinical factors associated with biochemical recurrence-free survival (BRFS). Univariate analyses were performed using the Kaplan-Meier and log-rank methods, and multivariate analysis was performed using a Cox proportional hazard model.
RESULTS: The median BChE level was 255 U/L (normal range 168-470 U/L). The median age of the enrolled patients was 68 years, and the median PSA level at diagnosis of PCa was 8.39 ng/mL. The median follow-up period was 65 months. The 5-year BRFS rate was 72.9 %. The 5-year BRFS rates in the BChE >/=168 and <= 167 U/L groups were 77.7 and 55.0 %, respectively (P < 0.001). In univariate analysis, BChE, cT, biopsy Gleason score, and D'Amico risk classification were significantly associated with BRFS. Multivariate analysis revealed that BChE was significantly associated with BRFS.
CONCLUSIONS: This study validated preoperative serum BChE levels as an independent prognostic factor for PCa after RP.
ESTHER : Koie_2016_Int.J.Clin.Oncol_21_379
PubMedSearch : Koie_2016_Int.J.Clin.Oncol_21_379
PubMedID: 26223693

Title : Preoperative butyrylcholinesterase level as an independent predictor of overall survival in clear cell renal cell carcinoma patients treated with nephrectomy - Koie_2014_ScientificWorldJournal_2014_948305
Author(s) : Koie T , Ohyama C , Mikami J , Iwamura H , Fujita N , Sato T , Kojima Y , Fukushi K , Yamamoto H , Imai A , Hatakeyama S , Yoneyama T , Hashimoto Y , Kitayama M , Hirota K
Ref : ScientificWorldJournal , 2014 :948305 , 2014
Abstract : The prognostic factors for the overall survival (OS) of clear cell renal cell carcinoma (ccRCC) patients treated with nephrectomy are not well defined. In the present study, we investigated the prognostic significance of preoperative butyrylcholinesterase (BChE) levels in 400 ccRCC patients undergoing radical or partial nephrectomy from 1992 to 2013 at our institution. Univariate and multivariate analyses were performed to determine the clinical factors associated with OS. Among the enrolled patients, 302 were diagnosed with organ-confined disease only (T1-2N0M0), 16 with lymph node metastases, and 56 with distant metastases. The median preoperative BChE level was 250 U/L (normal range, 168-470 U/L), and median follow-up period was 36 months. The 3-year OS rate in patients with preoperative BChE levels of >/=100 U/L was significantly higher than in those with levels of <100 U/L (89.3% versus 77.7%, P = 0.004). On univariate analysis, performance status; anemia; hypoalbuminemia; preoperative levels of BChE, corrected calcium, and C-reactive protein; and distant metastasis status were significantly associated with OS. Multivariate analysis revealed that preoperative BChE levels and distant metastasis status were significantly associated with OS. Our findings suggest a possible role of preoperative BChE levels as an independent predictor of OS after nephrectomy in ccRCC patients.
ESTHER : Koie_2014_ScientificWorldJournal_2014_948305
PubMedSearch : Koie_2014_ScientificWorldJournal_2014_948305
PubMedID: 24741368

Title : Significance of preoperative butyrylcholinesterase as an independent predictor of survival in patients with muscle-invasive bladder cancer treated with radical cystectomy - Koie_2014_Urol.Oncol_32_820
Author(s) : Koie T , Ohyama C , Yamamoto H , Hatakeyama S , Imai A , Yoneyama T , Hashimoto Y , Kitayam M , Hirota K
Ref : Urol Oncol , 32 :820 , 2014
Abstract : OBJECTIVES: Butyrylcholinesterase (BChE) is an alpha-glycoprotein found in the nervous system and liver. Its serum level is reduced in many clinical conditions, such as liver damage, inflammation, injury, infection, malnutrition, and malignant disease. In this study, we analyzed the potential prognostic significance of preoperative BChE levels in patients with muscle-invasive bladder cancer (MIBC) undergoing radical cystectomy (RC). METHODS AND MATERIALS: We retrospectively evaluated 327 patients with MIBC who underwent RC from 1996 to 2013 at a single institution. Serum BChE level was routinely measured before operation in all patients. Covariates included age, gender, preoperative laboratory data (anemia, BChE, lactate dehydrogenase, and C-reactive protein), clinical T (cT) and N stage (cN), tumor grade, and RC with/without neoadjuvant chemotherapy. Univariate and multivariate analyses were performed to identify clinical factors associated with overall survival (OS) and disease-free survival (DFS). Univariate analyses were performed using the Kaplan-Meier and log-rank methods, and the multivariate analysis was performed using a Cox proportional hazard model.
RESULTS: The median BChE level was 187U/l (normal range: 168-470U/l). The median age of the enrolled patients was 69 years, and the median follow-up period was 51 months. The 5-year OS and DFS rates were 69.6% and 69.3%, respectively. The 5-year OS rates were 90.1% and 51.3% in the BChE>/=168 and<168U/l groups, respectively (P<0.001). The 5-year DFS rates were 83.5% and 55.4% in the BChE>/=168 and</=167U/l groups, respectively (P<0.001). In the univariate analysis, BChE, cT, cN, and RC with/without neoadjuvant chemotherapy were significantly associated with both OS and DFS. Multivariate analysis revealed that BChE was the factor most significantly associated with OS, and BChE, cT, and cN were significantly associated with DFS.
CONCLUSIONS: This study validated preoperative serum BChE levels as an independent prognostic factor for MIBC after RC.
ESTHER : Koie_2014_Urol.Oncol_32_820
PubMedSearch : Koie_2014_Urol.Oncol_32_820
PubMedID: 24951323

Title : Convergence of nicotine-induced and auditory-evoked neural activity activates ERK in auditory cortex - Kawai_2013_Synapse_67_455
Author(s) : Kawai HD , La M , Kang HA , Hashimoto Y , Liang K , Lazar R , Metherate R
Ref : Synapse , 67 :455 , 2013
Abstract : Enhancement of sound-evoked responses in auditory cortex (ACx) following administration of systemic nicotine is known to depend on activation of extracellular-signaling regulated kinase (ERK), but the nature of this enhancement is not clear. Here, we show that systemic nicotine increases the density of cells immunolabeled for phosphorylated (activated) ERK (P-ERK) in mouse primary ACx (A1). Cortical injection of dihydro-beta-erythroidine reduced nicotine-induced P-ERK immunolabel, suggesting a role for nicotinic acetylcholine receptors located in A1 and containing alpha4 and beta2 subunits. P-ERK expressing cells were distributed mainly in layers 2/3 and more sparsely in lower layers, with many cells exhibiting immunolabel within pyramidal-shaped somata and proximal apical dendrites. About one-third of P-ERK positive cells also expressed calbindin. In the thalamus, P-ERK immunopositive cells were found in the nonlemniscal medial geniculate (MG) and adjacent nuclei, but were absent in the lemniscal MG. Pairing broad spectrum acoustic stimulation (white noise) with systemic nicotine increased P-ERK immunopositive cell density in ACx as well as the total amount of P-ERK protein, particularly the phosphorylated form of ERK2. However, narrow spectrum (tone) stimulation paired with nicotine increased P-ERK immunolabel preferentially at a site within A1 where the paired frequency was characteristic frequency (CF), relative to a second site with a spectrally distant CF (two octaves above or below the paired frequency). Together, these results suggest that ERK is activated optimally where nicotinic signaling and sound-evoked neural activity converge.
ESTHER : Kawai_2013_Synapse_67_455
PubMedSearch : Kawai_2013_Synapse_67_455
PubMedID: 23401204

Title : Genome sequence of Kitasatospora setae NBRC 14216T: an evolutionary snapshot of the family Streptomycetaceae - Ichikawa_2010_DNA.Res_17_393
Author(s) : Ichikawa N , Oguchi A , Ikeda H , Ishikawa J , Kitani S , Watanabe Y , Nakamura S , Katano Y , Kishi E , Sasagawa M , Ankai A , Fukui S , Hashimoto Y , Kamata S , Otoguro M , Tanikawa S , Nihira T , Horinouchi S , Ohnishi Y , Hayakawa M , Kuzuyama T , Arisawa A , Nomoto F , Miura H , Takahashi Y , Fujita N
Ref : DNA Research , 17 :393 , 2010
Abstract : Kitasatospora setae NBRC 14216(T) (=KM-6054(T)) is known to produce setamycin (bafilomycin B1) possessing antitrichomonal activity. The genus Kitasatospora is morphologically similar to the genus Streptomyces, although they are distinguishable from each other on the basis of cell wall composition and the 16S rDNA sequence. We have determined the complete genome sequence of K. setae NBRC 14216(T) as the first Streptomycetaceae genome other than Streptomyces. The genome is a single linear chromosome of 8,783,278 bp with terminal inverted repeats of 127,148 bp, predicted to encode 7569 protein-coding genes, 9 rRNA operons, 1 tmRNA and 74 tRNA genes. Although these features resemble those of Streptomyces, genome-wide comparison of orthologous genes between K. setae and Streptomyces revealed smaller extent of synteny. Multilocus phylogenetic analysis based on amino acid sequences unequivocally placed K. setae outside the Streptomyces genus. Although many of the genes related to morphological differentiation identified in Streptomyces were highly conserved in K. setae, there were some differences such as the apparent absence of the AmfS (SapB) class of surfactant protein and differences in the copy number and variation of paralogous components involved in cell wall synthesis.
ESTHER : Ichikawa_2010_DNA.Res_17_393
PubMedSearch : Ichikawa_2010_DNA.Res_17_393
PubMedID: 21059706
Gene_locus related to this paper: kitsk-e4n1a1 , kitsk-e4n2t9 , kitsk-e4n4j8 , kitsk-e4n6l6 , kitsk-e4n9v4 , kitsk-e4n533 , kitsk-e4n648 , kitsk-e4na97 , kitsk-e4nas1 , kitsk-e4nas4 , kitsk-e4nf33 , kitsk-e4ngi3 , kitsk-e4nj77 , kitsk-e4ngr7 , kitsk-e4n1n8 , kitsk-e4n5j9 , kitsk-e4ndj5 , kitsk-e4ni45 , kitsk-e4nik2

Title : A fatal poisoning caused by methomyl and nicotine - Moriya_2005_Forensic.Sci.Int_149_167
Author(s) : Moriya F , Hashimoto Y
Ref : Forensic Science International , 149 :167 , 2005
Abstract : A 35-year-old male was found lying in a prone position in his room. He was in cardiopulmonary arrest on arrival to hospital and was pronounced dead. There was no attempt at resuscitation. No miosis was observed on admission. At post-mortem his stomach contained 170 g greenish liquid with a small amount of shredded tobacco leaves. The serum cholinesterase activities were 47-90 IU (normal range for male: 200-440 IU). GC and GC-MS analyses showed nicotine (21.8 mg), methomyl (304 mg), and triazolam (1.69 mg) in his stomach. He had consumed tobacco leaves, Lannate containing water soluble methomyl (45%), and Halcion tablets containing 0.25 mg triazolam. Methomyl concentrations in blood were 3-8 ng/ml. Substantial amounts of methomyl (2260-2680 ng/ml) were detected in cerebrospinal fluid and vitreous humor. Nicotine concentrations in blood ranged from 222 to 733 ng/ml. A small amount of triazolam was detected only in bile (176 ng/ml) and liver (23 ng/g). The cause of death was respiratory paralysis produced by the additive effects of methomyl and nicotine shortly after consumption.
ESTHER : Moriya_2005_Forensic.Sci.Int_149_167
PubMedSearch : Moriya_2005_Forensic.Sci.Int_149_167
PubMedID: 15749358

Title : Inhibition of human plasma cholinesterase and erythrocyte acetylcholinesterase by nondepolarizing neuromuscular blocking agents - Kato_2000_J.Anesth_14_30
Author(s) : Kato M , Hashimoto Y , Horinouchi T , Ando T , Ito J , Yamanaka H
Ref : J Anesth , 14 :30 , 2000
Abstract : PURPOSE: The kinetics of the inhibition of human plasma cholinesterase (ChE) and erythrocyte acetylcholinesterase (AChE) by alcuronium, atracurium, d-tubocurarine, pancuronium, pipecuronium, and vecuronium were studied in blood drawn from 35 surgical patients.
METHODS: The activities of plasma ChE and erythrocyte AChE were determined by the calorimetric method of Ellman et al., using acetylthiocholine as the substrate. Lineweaver-Burk plots and Dixon plots were used for the analysis of the kinetics of both enzymes.
RESULTS: The dissociation constants (K(m)) of plasma ChE and erythrocyte AChE were 5.00 x 10(-5) M and 5.28 x 10(-5) M, respectively, indicating that both enzymes have similar affinity to acetylthiocholine. Both Lineweaver-Burk plots and Dixon plots indicated that the six nondepolarizing neuromuscular blocking agents (NMBAs) at different concentrations induce linear mixed-type inhibition. The apparent inhibition constants (K(i)) of pancuronium (8.72 x 10(-8) M) and vecuronium (3.53 x 10(-7) M) for plasma ChE inhibition were lower than that of neostigmine (7.36 x 10(-7) M), whereas those of the six nondepolarizing NMBAs for erythrocyte AChE were markedly higher than that of neostigmine.
CONCLUSIONS: Both plasma ChE and erythrocyte AChE were inhibited by six nondepolarizing NMBAs, and the pattern of inhibition of both enzymes was of mixed type. The inhibitory potencies of pancuronium and vecuronium for plasma ChE were larger than that of neostigmine, whereas those of the six nondepolarizing NMBAs for erythrocyte AChE were markedly lower than that of neostigmine. The rank order of relative potency for plasma ChE was pancuronium > vecuronium > pipecuronium > alcuronium > d-tubocurarine > atracurium.
ESTHER : Kato_2000_J.Anesth_14_30
PubMedSearch : Kato_2000_J.Anesth_14_30
PubMedID: 14564607

Title : Comparative studies on tissue distributions of organophosphorus, carbamate and organochlorine pesticides in decedents intoxicated with these chemicals - Moriya_1999_J.Forensic.Sci_44_1131
Author(s) : Moriya F , Hashimoto Y
Ref : J Forensic Science , 44 :1131 , 1999
Abstract : This paper describes the tissue distributions of dichlorvos, an organophosphate, chlorpyrifos-methyl, an organophosphorothioate, methomyl, a carbamate, and endrin, an organochlorine, in three individuals (Cases 1-3) who died after ingesting insecticidal preparations containing these chemicals. In Case 1 involving dichlorvos and chlorpyrifos-methyl, no dichlorvos was detected in most of the blood and tissue samples. Tiny amounts of dichlorvos (0.067 mg/L and 0.027 mg/L) were detected in the vitreous humor and cerebrospinal fluid, respectively. The chlorpyrifos-methyl concentrations in the blood samples were very site-dependent with a range of 0.615-2.24 mg/L. The tissue concentrations of chlorpyrifos-methyl were within the range 0.379-8.60 mg/kg. The total amounts of dichlorvos and chlorpyrifos-methyl in the stomach were 879 and 612 mg, respectively. The serum cholinesterase activity was 3 IU/L/37 degrees C. In Case 2 involving methomyl, the methomyl concentrations in the blood samples were very site-dependent with a range of 0.56-4.75 mg/L. The tissue concentrations of methomyl were 2.61 mg/kg or less, no methomyl being detected in the spleen, liver and kidney. The methomyl concentrations in the cerebrospinal fluid and vitreous humor were 5.37 and 4.75 mg/L, respectively. The stomach contained 85 mg methomyl. The serum cholinesterase activity was 73 IU/L/37 degrees C. In Case 3 involving endrin, the victim underwent medical treatment for 7 h after ingesting an endrin preparation. The differences in the endrin concentrations among the blood samples were small, with a range of 0.353-0.615 mg/L. The tissue concentrations of endrin were within the range 0.467-13.3 mg/kg. The endrin in the stomach (66 mg) was adsorbed almost completely on the activated charcoal that was administered for medical treatment.
ESTHER : Moriya_1999_J.Forensic.Sci_44_1131
PubMedSearch : Moriya_1999_J.Forensic.Sci_44_1131
PubMedID: 10582355

Title : An evaluation of neuromuscular reversal with edrophonium in a patient with malathion intoxication - Matsukawa_1997_Tohoku.J.Exp.Med_181_467
Author(s) : Matsukawa S , Hashimoto Y , Kato M , Hoshi K , Satoh D , Horinouchi T , Satoh S , Saishu T
Ref : Tohoku J Exp Med , 181 :467 , 1997
Abstract : We evaluated the neuromuscular reversal with edrophonium using peripheral nerve stimulator and recorder in a patient with malathion intoxication. Edrophonium 10 mg i.v. caused an increase in single twitch tension by 76% of the control during the recovery phase from an acute cholinergic crisis 16 days after ingestion of malathion solution. The present study indicated that edrophonium test seems to be a reliable monitoring in evaluating neuromuscular reversal in the patient with acute malathion insecticide poisoning.
ESTHER : Matsukawa_1997_Tohoku.J.Exp.Med_181_467
PubMedSearch : Matsukawa_1997_Tohoku.J.Exp.Med_181_467
PubMedID: 9210253

Title : Neuromuscular blocking actions of the aminoglycoside antibiotics sisomicin and micronomicin in the rabbit - Matsukawa_1997_Tohoku.J.Exp.Med_181_471
Author(s) : Matsukawa S , Suh JH , Hashimoto Y , Kato M , Satoh D , Saito S , Endo K , Saishu T
Ref : Tohoku J Exp Med , 181 :471 , 1997
Abstract : The neuromuscular blocking actions of sisomicin sulfate (SISO), micronomicin sulfate (MCR) and d-tubocurarine (dTc) were studied in 20 rabbits anesthetized with halothane. The i.v. administration of SISO 20-40 mg/kg, MCR 40-80 mg/kg or dTc 0.1-0.3 mg/kg resulted in dose-dependent decreases in twitch tension. The ED50s for SISO, MCR and dTc were 23.5, 58.2 and 0.2 mg/kg, respectively. SISO- and MCR-induced neuromuscular blockade was partially antagonized by neostigmine or by calcium.
ESTHER : Matsukawa_1997_Tohoku.J.Exp.Med_181_471
PubMedSearch : Matsukawa_1997_Tohoku.J.Exp.Med_181_471
PubMedID: 9210254

Title : Purification, characterization, and crystallization of two types of lipase from Rhizopus niveus - Kohno_1994_Biosci.Biotechnol.Biochem_58_1007
Author(s) : Kohno M , Kugimiya W , Hashimoto Y , Morita Y
Ref : Biosci Biotechnol Biochem , 58 :1007 , 1994
Abstract : The purification and some properties of two types of lipase (Lipase I and Lipase II) from Rhizopus niveus are described. The enzymes were purified to homogeneity by column chromatographies on DEAE-Toyopearl (1 pass) and CM-Toyopearl (2 passes). Lipase I consists of two polypeptide chains [a small peptide with sugar moiety (A-chain) and a large peptide of molecular weight 34,000 (B-chain)]. Lipase II has a molecular weight of 30,000 consisting of a single polypeptide chain. Lipase I appeared to be converted to Lipase II by limited proteolysis by a specific protease a small amount of which is in the culture supernatant from Rh. niveus, because one of the peptides formed has the same N-terminal sequence and C-terminal amino acid as Lipase II, as well as the molecular mass estimated by SDS-PAGE. Lipase I had a pH optimum of 6.0-6.5 and a temperature optimum of 35 degrees C, while, for Lipase II these values were pH 6.0 and 40 degrees C. Both enzymes were obtained in the crystalline state using the hanging drop method of vapor diffusion and PEG as the precipitating agents.
ESTHER : Kohno_1994_Biosci.Biotechnol.Biochem_58_1007
PubMedSearch : Kohno_1994_Biosci.Biotechnol.Biochem_58_1007
PubMedID: 7765029

Title : Preliminary investigation of crystals of lipase I from Rhizopus niveus - Kohno_1993_J.Mol.Biol_229_785
Author(s) : Kohno M , Kugimiya W , Hashimoto Y , Morita Y
Ref : Journal of Molecular Biology , 229 :785 , 1993
Abstract : Lipase I from Rhizopus niveus consists of two polypeptide chains bound non-covalently. Lipase I has been crystallized in a form suitable for X-ray diffraction analysis using the hanging drop method of vapour diffusion at 20 degrees C. The crystals grew at pH 6.0 to 7.0 using 14 to 16% polyethylene glycol 8000 as the precipitant. The crystals are tetragonal with space group P4(1) (or P4(3)) and cell dimensions of a = b = 83.7 A, c = 137.9 A. There are two protein molecules in the asymmetric unit. The diffraction pattern extends to at least 2.5 A resolution.
ESTHER : Kohno_1993_J.Mol.Biol_229_785
PubMedSearch : Kohno_1993_J.Mol.Biol_229_785
PubMedID: 8433372

Title : Molecular cloning and structure of the gene for esterase from a thermophilic bacterium, Bacillus stearothermophilus IFO 12550 -
Author(s) : Kugimiya W , Otani Y , Hashimoto Y
Ref : Biosci Biotechnol Biochem , 56 :2074 , 1992
PubMedID: 1369099
Gene_locus related to this paper: geost-est30

Title : Cloning and sequence analysis of cDNA encoding Rhizopus niveus lipase - Kugimiya_1992_Biosci.Biotechnol.Biochem_56_716
Author(s) : Kugimiya W , Otani Y , Kohno M , Hashimoto Y
Ref : Biosci Biotechnol Biochem , 56 :716 , 1992
Abstract : Complementary DNA encoding Rhizopus niveus lipase (RNL) was isolated from the R. niveus IF04759 cDNA library using a synthetic oligonucleotide corresponding to the amino acid sequence of the enzyme. A clone, which had an insert of 1.0 kilobase pairs, was found to contain the coding region of the enzyme. The lipase gene was expressed in Escherichia coli as a lacZ fusion protein. The mature RNL consisted of 297 amino acid residues with a molecular mass of 32 kDa. The RNL sequence showed significant overall homology to Rhizomucor miehei lipase and the putative active site residues were strictly conserved.
ESTHER : Kugimiya_1992_Biosci.Biotechnol.Biochem_56_716
PubMedSearch : Kugimiya_1992_Biosci.Biotechnol.Biochem_56_716
PubMedID: 1368341
Gene_locus related to this paper: rhidl-lipas

Title : Molecular cloning and nucleotide sequence of the lipase gene from Pseudomonas fragi - Kugimiya_1986_Biochem.Biophys.Res.Commun_141_185
Author(s) : Kugimiya W , Otani Y , Hashimoto Y , Takagi Y
Ref : Biochemical & Biophysical Research Communications , 141 :185 , 1986
Abstract : The gene coding for the lipase of Pseudomonas fragi was cloned into Escherichia coli JM83 by inserting Sau3A-generated DNA fragments into the BamH I site of pUC9. The plasmid isolated, pKKO, was restriction mapped and the position of the lipase gene on the 2.0 kb insert was pinpointed by subcloning. DNA sequencing revealed that the open reading frame comprises 405 nucleotides and gives a preprotein of 135 amino acids with a predicted Mr of 14643. By comparing the putative lipase amino acid sequence with porcine pancreatic, rat lingual and Staphylococcus hyicus lipases the amino acid sequence around the reactive serine was found to be common among the types of lipase which have been reported.
ESTHER : Kugimiya_1986_Biochem.Biophys.Res.Commun_141_185
PubMedSearch : Kugimiya_1986_Biochem.Biophys.Res.Commun_141_185
PubMedID: 3800995