Ohnishi Y

References (7)

Title : The alpha\/beta hydrolase AzpM catalyzes dipeptide synthesis in alazopeptin biosynthesis using two molecules of carrier protein-tethered amino acid - Kawai_2022_Chembiochem__
Author(s) : Kawai S , Katsuyama Y , Ohnishi Y
Ref : Chembiochem , : , 2022
Abstract : During the biosynthesis of alazopeptin, a tripeptide composed of two molecules of 6-diazo-5-oxo-L-norleucine (DON) and one of alanine, the alpha/beta hydrolase AzpM synthesizes the DON-DON dipeptide using DON tethered to the carrier protein AzpF (DON-AzpF). However, whether AzpM catalyzes the condensation of DON-AzpF with DON or DON-AzpF remains unclear. Here, to distinguish between these two condensation possibilities, the reaction catalyzed by AzpM was examined in vitro using a DON analog, azaserine (AZS). We found that AzpM catalyzed the condensation between AZS-AzpF and DON-AzpF, but not between AZS-AzpF and DON. Possible reaction intermediates, DON-DON-AzpF and AZS-AZS-AzpF, were also detected during AzpM-catalyzed dipeptide formation from DON-AzpF and AZS-AzpF, respectively. From these results, we concluded that AzpM catalyzed the condensation of the two molecules of DON-AzpF and subsequent hydrolysis to produce DON-DON. Thus, AzpM is an unprecedented alpha/beta hydrolase that catalyzes dipeptide synthesis from two molecules of a carrier protein-tethered amino acid.
ESTHER : Kawai_2022_Chembiochem__
PubMedSearch : Kawai_2022_Chembiochem__
PubMedID: 35132756
Gene_locus related to this paper: strgu-AzpM

Title : Complete Biosynthetic Pathway of Alazopeptin, a Tripeptide Consisting of Two Molecules of 6-Diazo-5-oxo-l-norleucine and One Molecule of Alanine - Kawai_2021_Angew.Chem.Int.Ed.Engl_60_10319
Author(s) : Kawai S , Sugaya Y , Hagihara R , Tomita H , Katsuyama Y , Ohnishi Y
Ref : Angew Chem Int Ed Engl , 60 :10319 , 2021
Abstract : DON (6-diazo-5-oxo-l-norleucine), a diazo-containing amino acid, has been studied for more than 60years as a potent antitumor agent, but its biosynthesis has not been elucidated. Here we reveal the complete biosynthetic pathway of alazopeptin, the tripeptide Ala-DON-DON, which has antitumor activity, by gene inactivation and in vitro analysis of recombinant enzymes. We also established heterologous production of N-acetyl-DON in Streptomyces albus. DON is synthesized from lysine by three enzymes and converted to alazopeptin by five enzymes and one carrier protein. Most interestingly, transmembrane protein AzpL was indicated to catalyze diazotization using 5-oxolysine and nitrous acid as substrates. Site-directed mutagenesis of AzpL indicated that the hydroxy group of Tyr-93 is important for the diazotization. These findings expand our knowledge of the enzymology of N-N bond formation.
ESTHER : Kawai_2021_Angew.Chem.Int.Ed.Engl_60_10319
PubMedSearch : Kawai_2021_Angew.Chem.Int.Ed.Engl_60_10319
PubMedID: 33624374
Gene_locus related to this paper: strgu-AzpM

Title : Mass Spectrometric Enzyme Histochemistry for Choline Acetyltransferase Reveals De Novo Acetylcholine Synthesis in Rodent Brain and Spinal Cord - Takeo_2021_ACS.Chem.Neurosci__
Author(s) : Takeo E , Sugiura Y , Ohnishi Y , Kishima H , Fukusaki E , Shimma S
Ref : ACS Chem Neurosci , : , 2021
Abstract : Choline acetyltransferase (ChAT), responsible for the synthesis of acetylcholine, plays an important role in neurotransmission. However, no method to visualize the ChAT activity in tissues has been reported to date. In this study, mass spectrometry imaging (MSI) was used to visualize ChAT activity in situ, which is difficult with conventional enzyme histochemistry. By using choline chloride-trimethyl-d9 (choline-d9) as a substrate and simultaneously supplying an inhibitor of cholinesterase to tissues, we succeeded in directly visualizing the ChAT activity in the rodent brain and spinal cord. The findings revealed heterogeneous ChAT activity in the striatum of the mouse brain and in the spinal lower motor neurons that connect the anterior horn to the ventral root. Furthermore, extending the developed method to spinal cord injury (SCI) model mice revealed the site-specific effect of primary and secondary injury on ChAT activity. This study shows that the MSI-based enzyme histochemistry of ChAT could be a useful tool for studying cholinergic neurons.
ESTHER : Takeo_2021_ACS.Chem.Neurosci__
PubMedSearch : Takeo_2021_ACS.Chem.Neurosci__
PubMedID: 34078081

Title : Genome sequence of Kitasatospora setae NBRC 14216T: an evolutionary snapshot of the family Streptomycetaceae - Ichikawa_2010_DNA.Res_17_393
Author(s) : Ichikawa N , Oguchi A , Ikeda H , Ishikawa J , Kitani S , Watanabe Y , Nakamura S , Katano Y , Kishi E , Sasagawa M , Ankai A , Fukui S , Hashimoto Y , Kamata S , Otoguro M , Tanikawa S , Nihira T , Horinouchi S , Ohnishi Y , Hayakawa M , Kuzuyama T , Arisawa A , Nomoto F , Miura H , Takahashi Y , Fujita N
Ref : DNA Research , 17 :393 , 2010
Abstract : Kitasatospora setae NBRC 14216(T) (=KM-6054(T)) is known to produce setamycin (bafilomycin B1) possessing antitrichomonal activity. The genus Kitasatospora is morphologically similar to the genus Streptomyces, although they are distinguishable from each other on the basis of cell wall composition and the 16S rDNA sequence. We have determined the complete genome sequence of K. setae NBRC 14216(T) as the first Streptomycetaceae genome other than Streptomyces. The genome is a single linear chromosome of 8,783,278 bp with terminal inverted repeats of 127,148 bp, predicted to encode 7569 protein-coding genes, 9 rRNA operons, 1 tmRNA and 74 tRNA genes. Although these features resemble those of Streptomyces, genome-wide comparison of orthologous genes between K. setae and Streptomyces revealed smaller extent of synteny. Multilocus phylogenetic analysis based on amino acid sequences unequivocally placed K. setae outside the Streptomyces genus. Although many of the genes related to morphological differentiation identified in Streptomyces were highly conserved in K. setae, there were some differences such as the apparent absence of the AmfS (SapB) class of surfactant protein and differences in the copy number and variation of paralogous components involved in cell wall synthesis.
ESTHER : Ichikawa_2010_DNA.Res_17_393
PubMedSearch : Ichikawa_2010_DNA.Res_17_393
PubMedID: 21059706
Gene_locus related to this paper: kitsk-e4n1a1 , kitsk-e4n2t9 , kitsk-e4n4j8 , kitsk-e4n6l6 , kitsk-e4n9v4 , kitsk-e4n533 , kitsk-e4n648 , kitsk-e4na97 , kitsk-e4nas1 , kitsk-e4nas4 , kitsk-e4nf33 , kitsk-e4ngi3 , kitsk-e4nj77 , kitsk-e4ngr7 , kitsk-e4n1n8 , kitsk-e4n5j9 , kitsk-e4ndj5 , kitsk-e4ni45 , kitsk-e4nik2

Title : Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350 - Ohnishi_2008_J.Bacteriol_190_4050
Author(s) : Ohnishi Y , Ishikawa J , Hara H , Suzuki H , Ikenoya M , Ikeda H , Yamashita A , Hattori M , Horinouchi S
Ref : Journal of Bacteriology , 190 :4050 , 2008
Abstract : We determined the complete genome sequence of Streptomyces griseus IFO 13350, a soil bacterium producing an antituberculosis agent, streptomycin, which is the first aminoglycoside antibiotic, discovered more than 60 years ago. The linear chromosome consists of 8,545,929 base pairs (bp), with an average G+C content of 72.2%, predicting 7,138 open reading frames, six rRNA operons (16S-23S-5S), and 66 tRNA genes. It contains extremely long terminal inverted repeats (TIRs) of 132,910 bp each. The telomere's nucleotide sequence and secondary structure, consisting of several palindromes with a loop sequence of 5'-GGA-3', are different from those of typical telomeres conserved among other Streptomyces species. In accordance with the difference, the chromosome has pseudogenes for a conserved terminal protein (Tpg) and a telomere-associated protein (Tap), and a novel pair of Tpg and Tap proteins is instead encoded by the TIRs. Comparisons with the genomes of two related species, Streptomyces coelicolor A3(2) and Streptomyces avermitilis, clarified not only the characteristics of the S. griseus genome but also the existence of 24 Streptomyces-specific proteins. The S. griseus genome contains 34 gene clusters or genes for the biosynthesis of known or unknown secondary metabolites. Transcriptome analysis using a DNA microarray showed that at least four of these clusters, in addition to the streptomycin biosynthesis gene cluster, were activated directly or indirectly by AdpA, which is a central transcriptional activator for secondary metabolism and morphogenesis in the A-factor (a gamma-butyrolactone signaling molecule) regulatory cascade in S. griseus.
ESTHER : Ohnishi_2008_J.Bacteriol_190_4050
PubMedSearch : Ohnishi_2008_J.Bacteriol_190_4050
PubMedID: 18375553
Gene_locus related to this paper: strgg-b1vku3 , strgg-b1vl52 , strgg-b1vlg6 , strgg-b1vm48 , strgg-b1vmn5 , strgg-b1vms2 , strgg-b1vmu0 , strgg-b1vn62 , strgg-b1vn76 , strgg-b1vnq8 , strgg-b1vp88 , strgg-b1vpj5.1 , strgg-b1vpj5.2 , strgg-b1vpn6 , strgg-b1vr06 , strgg-b1vr99 , strgg-b1vs60 , strgg-b1vsh1 , strgg-b1vsw0 , strgg-b1vt13 , strgg-b1vtd3 , strgg-b1vu88 , strgg-b1vw73 , strgg-b1vwl8 , strgg-b1vwx6 , strgg-b1vyg6 , strgg-b1vyh0 , strgg-b1vyj0 , strgg-b1vz78 , strgg-b1vzt4 , strgg-b1vzw6 , strgg-b1w1l1 , strgg-b1w1m6 , strgg-b1w2r7 , strgg-b1w3q4 , strgg-b1w4m3 , strgg-b1w5d5 , strgg-b1w5v0 , strgg-b1w021 , strgg-b1w150 , strgg-b1w151 , strgg-b1w254 , strgg-b1w1b1 , strgg-b1vkv5 , strgg-b1vqn4 , strgg-b1vv39

Title : Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation - Kuwahara_2004_Proc.Natl.Acad.Sci.U.S.A_101_14919
Author(s) : Kuwahara T , Yamashita A , Hirakawa H , Nakayama H , Toh H , Okada N , Kuhara S , Hattori M , Hayashi T , Ohnishi Y
Ref : Proc Natl Acad Sci U S A , 101 :14919 , 2004
Abstract : Bacteroides are predominant human colonic commensals, but the principal pathogenic species, Bacteroides fragilis (BF), lives closely associated with the mucosal surface, whereas a second major species, Bacteroides thetaiotaomicron (BT), concentrates within the colon. We find corresponding differences in their genomes, based on determination of the genome sequence of BF and comparative analysis with BT. Both species have acquired two mechanisms that contribute to their dominance among the colonic microbiota: an exceptional capability to use a wide range of dietary polysaccharides by gene amplification and the capacity to create variable surface antigenicities by multiple DNA inversion systems. However, the gene amplification for polysaccharide assimilation is more developed in BT, in keeping with its internal localization. In contrast, external antigenic structures can be changed more systematically in BF. Thereby, at the mucosal surface, where microbes encounter continuous attack by host defenses, BF evasion of the immune system is favored, and its colonization and infectious potential are increased.
ESTHER : Kuwahara_2004_Proc.Natl.Acad.Sci.U.S.A_101_14919
PubMedSearch : Kuwahara_2004_Proc.Natl.Acad.Sci.U.S.A_101_14919
PubMedID: 15466707
Gene_locus related to this paper: bacfn-q5lh43 , bacfr-q64mh3 , bacfr-q64n33 , bacfr-q64qs3 , bacfr-q64t24 , bacfr-q64uj8 , bacfr-q64vl4 , bacfr-q64vx6 , bacfr-q64wh2 , bacfr-q64xp9 , bacfr-q64yv4 , bacfr-q650j0

Title : Effect of donepezil on group II mGlu receptor agonist- or antagonist-induced amnesia on passive avoidance in mice - Sato_2003_Neural.Plast_10_319
Author(s) : Sato T , Tanaka K , Ohnishi Y , Irifune M , Nishikawa T
Ref : Neural Plast , 10 :319 , 2003
Abstract : We examined the effect of the acetylcholinesterase (AChE) inhibitor, donepezil hydrocloride (DONP), on group II metabotropic glutamate (mGlu) receptor agonist- or antagonist-induced amnesia in the step-through passive avoidance task in male mice. DCG-IV, a group II mGlu receptor agonist, at dose of 50 ng and LY341495, a group II mGlu receptor antagonist, at dose of 300 ng, significantly attenuated the latency on the step-through task. The subcutaneous injection of DONP at dose of 1 mg/kg 1 hour before passive avoidance performance ameliorated the amnesia induced by DCG-IV and LY341495, whereas donepezil alone did not affect task latency. The results suggest that activation of group II mGlu receptors and disinhibition of the cAMP/PKA signaling pathway (caused by group II mGlu receptor antagonist) have a negative action on step-through passive avoidance memory performance, and that group II mGlu receptors and ACh interact to modulate learning and memory function.
ESTHER : Sato_2003_Neural.Plast_10_319
PubMedSearch : Sato_2003_Neural.Plast_10_319
PubMedID: 15152985