References (2)

Title : OsMas1, a novel maspardin protein gene, confers the tolerance to salt and drought stresses by regulating ABA signaling in rice - Wang_2022_J.Integr.Agric__
Author(s) : Wang FB , Wan CZ , Niu HF , Qi MY , Li G , Zhang F , Hu LB , Ye YX , Wang ZX , Pei BL , Chen XH , Yuan CY
Ref : J.Integr.Agric , : , 2022
Abstract : Drought and salt stresses, as major environmental abiotic stresses in agriculture worldwide, affect plant growth and crop productivity and quality. The development of crops with higher drought and salt tolerance is therefore highly desirable. Here, we reported the isolation and biological function and molecular characterization of a novel maspardin gene OsMas1 from rice. The OsMas1 protein was localized to the cytoplasm. The expression levels of OsMas1 were up-regulated under mannitol, PEG6000, NaCl and ABA treatments in rice. The OsMas1 gene was introduced into rice cultivar Zhonghua 11 (wild-type, WT) and OsMas1-overexpression (OsMas1-OE) plants exhibited significantly enhanced salt and drought tolerance, while OsMas1-interference (OsMas1-RNAi) plants exhibited decreased tolerance to salt and drought stresses, compared with WT. OsMas1-OE plants exhibited enhanced hypersensitive, while OsMas1-RNAi plants showed less sensitive to exogenous ABA treatment at both germination and post-germination stages. ABA, proline and K+ content and superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and photosynthesis activities were significantly increased, while malonaldehyde (MDA), hydrogen peroxide (H2O2), superoxide anion radical (O2-) and Na+ content were significantly decreased in OsMas1-OE plants compared with OsMas1-RNAi and WT plants. Overexpression of OsMas1 up-regulated the genes involved in ABA signaling, proline biosynthesis, ROS-scavenging system, photosynthesis and ion transport under salt and drought stresses. Collectively, our results indicate that OsMas1 gene functions in improving salt and drought tolerance in rice, which may serve as a candidate gene for enhancing the resistance to abiotic stresses in crops.
ESTHER : Wang_2022_J.Integr.Agric__
PubMedSearch : Wang_2022_J.Integr.Agric__
Gene_locus related to this paper: orysa-q33aq0

Title : Microcystin-LR-induced phytotoxicity in rice crown root is associated with the cross-talk between auxin and nitric oxide - Chen_2013_Chemosphere_93_283
Author(s) : Chen J , Zhang HQ , Hu LB , Shi ZQ
Ref : Chemosphere , 93 :283 , 2013
Abstract : Irrigation with cyanobacterial-blooming water containing microcystin-LR (MC-LR) poses threat to the growth of agricultural plants. Large amounts of rice (Oryza sativa) field in the middle part of China has been irrigating with cyanobacterial-blooming water. Nevertheless, the mechanism of MC-LR-induced phytotoxicity in the root of monocot rice remains unclear. In the present study, we demonstrate that MC-LR stress significantly inhibits the growth of rice root by impacting the morphogenesis rice crown root. MC-LR treatment results in the decrease in IAA (indole-3-acetic acid) concentration as well as the expression of CRL1 and WOX11 in rice roots. The application of NAA (1-naphthylacetic acid), an IAA homologue, is able to attenuate the inhibitory effect of MC-LR on rice root development. MC-LR treatment significantly inhibits OsNia1-dependent NO generation in rice roots. The application of NO donor SNP (sodium nitroprusside) is able to partially reverse the inhibitory effects of MC-LR on the growth of rice root and the expression of CRL1 and WOX11 by enhancing endogenous NO level in rice roots. The application of NO scavenger cPTIO [2-(4-carboxy-2-phenyl)-4,4,5,5-tetramethylinidazoline-1-oxyl-3-oxide] eliminates the effects of SNP. Treatment with NAA stimulates the generation of endogenous NO in MC-LR-treated rice roots. Treatment with NO scavenger cPTIO abolishes the ameliorated effect of NAA on MC-LR-induced growth inhibition of rice root. Treatment with SNP enhanced IAA concentration in MC-LR-treated rice roots. Altogether, our data suggest that NO acts both downstream and upstream of auxin in regulating rice root morphogenesis under MC-LR stress.
ESTHER : Chen_2013_Chemosphere_93_283
PubMedSearch : Chen_2013_Chemosphere_93_283
PubMedID: 23726011