Wang ZX

References (9)

Title : Cornuside ameliorates cognitive impairments in scopolamine induced AD mice: Involvement of neurotransmitter and oxidative stress - Wang_2022_J.Ethnopharmacol__115252
Author(s) : Wang ZX , Lian WW , He J , He XL , Wang YM , Pan CH , Li M , Zhang WK , Liu LQ , Xu JK
Ref : J Ethnopharmacol , :115252 , 2022
Abstract : ETHNOPHARMACOLOGICAL RELEVANCE: Cornus officinalis Sieb. et Zucc., traditional Chinese medicine, has been widely used in the treatment of dementia. Cornel iridoid glycosides of Cornus officinalis is therapeutic to Alzheimer's disease (AD), while its pharmacodynamic material basis is not clear. Cornuside, an iridoid glycoside extracted from of Cornus officinalis Sieb. et Zucc, might be a potential anti-AD candidate. AIM OF THE STUDY: Cornuside was evaluated for its effect on scopolamine induced AD mice, and its action mechanisms were explored. MATERIALS AND METHODS: ICR mice were administered with 1 mg/kg scopolamine intraperitoneally to induce amnesia. The therapeutic effect of cornuside of cognitive function was evaluated via series of behavioral tests, including Morris water maze test, step-through test and step-down test. In addition, specific enzyme reaction tests were used to detect the content of acetylcholine (ACh) and malondialdehyde (MDA), as well as the activities of acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), choline acetyltransferase (ChAT), superoxide dismutase (SOD), catalase (CAT), monoamine oxidase (MAO) in the brain. The levels of monoamine neurotransmitters were detected by high performance liquid chromatography-electrochemical detection (HPLC-ECD). RESULTS: Cornuside ameliorated the spatial memory impairment in Morris water maze test and cognitive disruption in step-through and step-down test. Furthermore, cornuside improved the level of ACh by reducing the activities of AChE and BuChE, and increasing the activity of ChAT in hippocampus. Cornuside also increased the levels of monoamine neurotransmitters by inhibiting MAO activity in hippocampus and cortex. In addition, cornuside attenuated MDA by enhancing the activities of SOD and CAT in hippocampus and cortex. CONCLUSION: Cornuside improved cognitive dysfunction induced by scopolamine in behavioral tests. The mechanisms of cornuside were further investigated from the aspects of neurotransmitters and oxidative stress. Cornuside could inhibit oxidative stress and neurotransmitter hydrolases, increase ACh and monoamine neurotransmitters, which finally contributed to its therapeutic effect on scopolamine induced amnesia.
ESTHER : Wang_2022_J.Ethnopharmacol__115252
PubMedSearch : Wang_2022_J.Ethnopharmacol__115252
PubMedID: 35405255

Title : OsMas1, a novel maspardin protein gene, confers the tolerance to salt and drought stresses by regulating ABA signaling in rice - Wang_2022_J.Integr.Agric__
Author(s) : Wang FB , Wan CZ , Niu HF , Qi MY , Li G , Zhang F , Hu LB , Ye YX , Wang ZX , Pei BL , Chen XH , Yuan CY
Ref : J.Integr.Agric , : , 2022
Abstract : Drought and salt stresses, as major environmental abiotic stresses in agriculture worldwide, affect plant growth and crop productivity and quality. The development of crops with higher drought and salt tolerance is therefore highly desirable. Here, we reported the isolation and biological function and molecular characterization of a novel maspardin gene OsMas1 from rice. The OsMas1 protein was localized to the cytoplasm. The expression levels of OsMas1 were up-regulated under mannitol, PEG6000, NaCl and ABA treatments in rice. The OsMas1 gene was introduced into rice cultivar Zhonghua 11 (wild-type, WT) and OsMas1-overexpression (OsMas1-OE) plants exhibited significantly enhanced salt and drought tolerance, while OsMas1-interference (OsMas1-RNAi) plants exhibited decreased tolerance to salt and drought stresses, compared with WT. OsMas1-OE plants exhibited enhanced hypersensitive, while OsMas1-RNAi plants showed less sensitive to exogenous ABA treatment at both germination and post-germination stages. ABA, proline and K+ content and superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and photosynthesis activities were significantly increased, while malonaldehyde (MDA), hydrogen peroxide (H2O2), superoxide anion radical (O2-) and Na+ content were significantly decreased in OsMas1-OE plants compared with OsMas1-RNAi and WT plants. Overexpression of OsMas1 up-regulated the genes involved in ABA signaling, proline biosynthesis, ROS-scavenging system, photosynthesis and ion transport under salt and drought stresses. Collectively, our results indicate that OsMas1 gene functions in improving salt and drought tolerance in rice, which may serve as a candidate gene for enhancing the resistance to abiotic stresses in crops.
ESTHER : Wang_2022_J.Integr.Agric__
PubMedSearch : Wang_2022_J.Integr.Agric__
Gene_locus related to this paper: orysa-q33aq0

Title : The influence of in vitro gastrointestinal digestion on the Perilla frutescens leaf extract: Changes in the active compounds and bioactivities - Wang_2020_J.Food.Biochem__e13530
Author(s) : Wang ZX , Lin QQ , Tu ZC , Zhang L
Ref : J Food Biochem , :e13530 , 2020
Abstract : In this study, the influence of in vitro gastrointestinal digestion on the Perilla frutescens leaf extract (PFLE) were measured. Results revealed that total phenolic content (TPC) and total flavonoid content (TFC) were significantly decreased after simulated digestion (ca. 53% of phenolics and 40% of flavonoids). The IC(50) value of DPPH. scavenging activity and ABTS(+) scavenging ability increased by 23% and 56%, respectively, while ferric reducing antioxidant power reduced by 53%. For the inhibition ability on alpha-glucosidase, acetylcholinesterase, and MCF-7 cell proliferation, their IC(50) values increased by 360%, 197%, and 25%, respectively. Three phenolic acids and one flavonoid in PFLE were quantified by high-performance liquid chromatography. Overall, although significant losses of the active components and biological activities occurred during in vitro gastrointestinal digestion, it still showed the potential as an oral agent for treatment and prevention of oxidative stress, cancer, diabetes, and Alzheimer's disease. PRACTICAL APPLICATIONS: As an important annual herbaceous plant with rich biochemical compounds and many biological functions, Perilla frutescens leave is widely used in the food and traditional Chinese medicine. However, the dynamic changes of its active compounds and activities during the digestion process are unclear. In this study, the digestion results in significant loss of the active ingredients and biological activities of P. frutescens leaf extract (PFLE), particularly in the gastric digestion. In addition, PFLE remains to show certain antioxidant activity, alpha-glucosidase inhibitory ability, acetylcholinesterase inhibitory ability, and MCF-7 cell proliferation inhibitory ability after digestion. Therefore, this research might facilitate further research and development of P. frutescens.
ESTHER : Wang_2020_J.Food.Biochem__e13530
PubMedSearch : Wang_2020_J.Food.Biochem__e13530
PubMedID: 33084119

Title : Synthesis of flavor esters by a novel lipase from Aspergillus niger in a soybean-solvent system - Cong_2019_3.Biotech_9_244
Author(s) : Cong S , Tian K , Zhang X , Lu F , Singh S , Prior B , Wang ZX
Ref : 3 Biotech , 9 :244 , 2019
Abstract : To find a lipase for synthesis of flavor esters in food processing, a total of 35 putative lipases from Aspergillus niger F0215 were heterologously expressed and their esterification properties in crude preparations were examined. One of them, named An-lipase with the highest esterification rate (23.1%) was selected for further study. The purified An-lipase had the maximal activity at 20s degreesC and pH 6.5 and the specific activity of 1293sU/mg. Sixty percent of the activity was maintained in a range of temperatures of 0-30s degreesC and pHs of 3.0-8.5. The highest hydrolysis activity of An-lipase was towards pNPC (C8), followed by pNPB (C4) and pNPA (C2), then pNPL (C12). K (m), V (max), k (cat,) and k (cat)/K (m) towards pNPC were 26.7smmol/L, 129.9smmol/(Lsh), 23.2ss(-1), and 0.8/mM/s, respectively. The ethyl lactate, butyl butyrate, and ethyl caprylate flavor esters were produced by esterification of the corresponding acids with conversion efficiencies of 15.8, 37.5, and 24.7%, respectively, in a soybean-oil-based solvent system. In conclusion, An lipase identified in this study significantly mediated synthesis of predominant flavor esters (ethyl lactate, butyl butyrate, and ethyl caprylate) in a soybean-oil-lacking other toxic organic solvents, which has potential application in food industries.
ESTHER : Cong_2019_3.Biotech_9_244
PubMedSearch : Cong_2019_3.Biotech_9_244
PubMedID: 31168437
Gene_locus related to this paper: aspnc-cuti1

Title : Safflower yellow reduces lipid peroxidation, neuropathology, tau phosphorylation and ameliorates amyloid beta-induced impairment of learning and memory in rats - Ma_2015_Biomed.Pharmacother_76_153
Author(s) : Ma Q , Ruan YY , Xu H , Shi XM , Wang ZX , Hu YL
Ref : Biomed Pharmacother , 76 :153 , 2015
Abstract : Insoluble plaques of amyloid beta proteins (Abeta) and neurofibrillary tangles of hyperphosphorylated tau are key markers for Alzheimer's disease (AD). Safflower yellow (SY) is one of traditional Chinese medicine extracted from safflower, which is suggested to have therapeutic potential for neurodegenerative disorders. However, whether SY can ameliorate impairment of learning and memory in AD model, and its causal mechanism are still unclear. Here, we applied different doses of SY intragastrically to Wistar rats injected with amyloid beta (1-42) for 1 month. By the Morris water maze test, we found that treatment of SY significantly attenuated amyloid beta (1-42)-induced impairment of memory in rats. Mechanistically, SY treatment increased the level of superoxidedismutase (SOD) and Glutathione peroxidase (GSH-Px), and decreased the level of malondialdehyde (MDA) and acetylcholinesterase (T-CHE) in brain tissues of AD rats. Pathological analysis also showed that SY treatment inhibited the morphological alteration of neurons and tau hyperphosphorylation induced by amyloid beta (1-42)-injection in the cortex and hippocampus. Moreover, SY treatment inhibited CDK-5 and GSK-3 signaling pathways, which are upregulated in AD rats. Our data indicate that safflower yellow can serve as a therapeutic candidate for Alzheimer's disease.
ESTHER : Ma_2015_Biomed.Pharmacother_76_153
PubMedSearch : Ma_2015_Biomed.Pharmacother_76_153
PubMedID: 26653563

Title : [Two novel mutations in palmitoyl-protein thioesterase gene in two Chinese babies with infantile neuronal ceroid lipofuscinosis] - Bi_2006_Zhonghua.Er.Ke.Za.Zhi_44_496
Author(s) : Bi HY , Yao S , Bu DF , Wang ZX , Zhang Y , Qin J , Yang YL , Yuan Y
Ref : Zhonghua Er Ke Za Zhi , 44 :496 , 2006
Abstract : OBJECTIVE: To search for possible novel mutations in palmitoyl-protein thioesterase 1 (PPT1) gene in two Chinese babies with infantile neuronal ceroid lipofuscinosis (INCL).
METHODS: Two probands with INCL, confirmed clinically and pathologically, were used for mutation search in PPT1 gene. Onset of the disease occurred before the age of 1 year and they mainly showed progressive mental and motor retardation. The 9 coding exons and their flanking intron sequences of palmitoyl-protein thioesterase 1 (PPT1) gene were amplified by using PCR and sequenced. The parents of proband 1 were also examined.
RESULTS: One splicing mutation and two missense mutations were identified in the two probands: the proband 1 carrying a compound heterozygous mutation of a IVS1 + 1G-->A mutation in intron 1 and a c550G-->A mutation in exon 6 leading to the amino acid substitution of E184K. Additionally, the parents of the proband 1 also harbored one of the mutations of the patient, respectively. The proband 2 carrying a homozygous mutation of c272A-->C in exon 3, which resulted in the amino acid substitutions of Q91P.
CONCLUSIONS: The IVS1 + 1G-->A mutation and Q91P mutation are novel mutations, which lead to INCL. The genetic abnormalities of PPT1 in Chinese patients may not be completely the same as those in the patients of other regions of the world.
ESTHER : Bi_2006_Zhonghua.Er.Ke.Za.Zhi_44_496
PubMedSearch : Bi_2006_Zhonghua.Er.Ke.Za.Zhi_44_496
PubMedID: 17044973
Gene_locus related to this paper: human-PPT1

Title : [A study on the inherited susceptibility of chromosomal damage in peripheral blood lymphocytes among coke oven workers] - Leng_2004_Zhonghua.Yu.Fang.Yi.Xue.Za.Zhi_38_94
Author(s) : Leng SG , Zheng YX , Pan ZF , Niu Y , Dai YF , Wang YW , Zhang WZ , Xiao J , Wang ZX , Li T , He FS
Ref : Zhonghua Yu Fang Yi Xue Za Zhi , 38 :94 , 2004
Abstract : OBJECTIVE: To investigate the association between polymorphisms of metabolic enzyme genes and chromosomal damage risk in peripheral blood lymphocytes among coke oven workers.
METHODS: One hundred and fourty-nine coke oven workers and 24 referents without occupational polycyclic aromatic hydrocarbons (PAH) exposure were recruited in this study. Urinary 1-hydroxypyrene levels were measured as the internal dose of PAH exposure. The 6 per 1 000 of micronucleus value was used as the cut-off value to determine whether the individual's chromosomal damage was positive. The genotypes of CYP1A1, GSTM1, GSTT1, GSTP1, CYP2E1, NQO1, NAT2 and mEH genes were determined by PCR-based methods. Multiple logistic regression was used to calculate the adjusted ORs and the 95% CI for the risk of chromosomal damage and to analyze the gene-gene interaction.
RESULTS: In 173 subjects, after adjusting the occupational exposure, age, sex, smoking and drinking status, the subjects with GSTM1 null genotype have significantly higher risk for chromosomal damage than subjects with GSTM1 positive genotype (adjusted OR = 2.01, 95% CI = 1.03 -3.91). Compared with the wild homozygotes at P187S site of NQO1 gene, the variant homozygotes have significantly higher risk for chromosomal damage (adjusted OR = 3.18, 95% CI = 1.18 - 8.62). The subjects with variant allele at H113Y site of mEH gene have significantly lower risk for chromosomal damage (adjusted OR = 0.40, 95% CI = 0.19 - 0.88). No significant associations were found for other gene polymorphisms and chromosomal damage risk. In addition, the gene-gene interactions were also found among GSTM1, NQO1 gene P187S and mEH gene H113Y polymorphisms for the risk of chromosomal damage risk. CONCLUSION: Significant associations between genetic polymorphisms in GSTM1, NQO1 and mEH gene and risk for chromosomal damage were found among occupational PAH-exposed workers, which related to the mechanism of PAH carcinogenesis.
ESTHER : Leng_2004_Zhonghua.Yu.Fang.Yi.Xue.Za.Zhi_38_94
PubMedSearch : Leng_2004_Zhonghua.Yu.Fang.Yi.Xue.Za.Zhi_38_94
PubMedID: 15061915

Title : Identification of the coumermycin A(1) biosynthetic gene cluster of Streptomyces rishiriensis DSM 40489 - Wang_2000_Antimicrob.Agents.Chemother_44_3040
Author(s) : Wang ZX , Li SM , Heide L
Ref : Antimicrobial Agents & Chemotherapy , 44 :3040 , 2000
Abstract : The biosynthetic gene cluster of the aminocoumarin antibiotic coumermycin A(1) was cloned by screening of a cosmid library of Streptomyces rishiriensis DSM 40489 with heterologous probes from a dTDP-glucose 4,6-dehydratase gene, involved in deoxysugar biosynthesis, and from the aminocoumarin resistance gyrase gene gyrB(r). Sequence analysis of a 30.8-kb region upstream of gyrB(r) revealed the presence of 28 complete open reading frames (ORFs). Fifteen of the identified ORFs showed, on average, 84% identity to corresponding ORFs in the biosynthetic gene cluster of novobiocin, another aminocoumarin antibiotic. Possible functions of 17 ORFs in the biosynthesis of coumermycin A(1) could be assigned by comparison with sequences in GenBank. Experimental proof for the function of the identified gene cluster was provided by an insertional gene inactivation experiment, which resulted in an abolishment of coumermycin A(1) production.
ESTHER : Wang_2000_Antimicrob.Agents.Chemother_44_3040
PubMedSearch : Wang_2000_Antimicrob.Agents.Chemother_44_3040
PubMedID: 11036020
Gene_locus related to this paper: strri-CUML

Title : [Effect of soman, sarin and VX on specific binding of 3H-quinuclidinyl benzilate in rat cerebral cortex homogenates] -
Author(s) : Zhao DL , Wang ZX , Pei SQ , Liu CH
Ref : Acta Pharmacol Sin , 4 :225 , 1983
PubMedID: 6230862