Lester JB

References (2)

Title : Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338 - Oliynyk_2007_Nat.Biotechnol_25_447
Author(s) : Oliynyk M , Samborskyy M , Lester JB , Mironenko T , Scott N , Dickens S , Haydock SF , Leadlay PF
Ref : Nat Biotechnol , 25 :447 , 2007
Abstract : Saccharopolyspora erythraea is used for the industrial-scale production of the antibiotic erythromycin A, derivatives of which play a vital role in medicine. The sequenced chromosome of this soil bacterium comprises 8,212,805 base pairs, predicted to encode 7,264 genes. It is circular, like those of the pathogenic actinomycetes Mycobacterium tuberculosis and Corynebacterium diphtheriae, but unlike the linear chromosomes of the model actinomycete Streptomyces coelicolor A3(2) and the closely related Streptomyces avermitilis. The S. erythraea genome contains at least 25 gene clusters for production of known or predicted secondary metabolites, at least 72 genes predicted to confer resistance to a range of common antibiotic classes and many sets of duplicated genes to support its saprophytic lifestyle. The availability of the genome sequence of S. erythraea will improve insight into its biology and facilitate rational development of strains to generate high-titer producers of clinically important antibiotics.
ESTHER : Oliynyk_2007_Nat.Biotechnol_25_447
PubMedSearch : Oliynyk_2007_Nat.Biotechnol_25_447
PubMedID: 17369815
Gene_locus related to this paper: sacen-a4f6m6 , sacen-a4f7j2 , sacen-a4f7j9 , sacen-a4f8h3 , sacen-a4f8m4 , sacen-a4f8u9 , sacen-a4f9g1 , sacen-a4f9k7 , sacen-a4f815 , sacen-a4f824 , sacen-a4fam2 , sacen-a4faz0 , sacen-a4fbh5 , sacen-a4fbt1 , sacen-a4fc70 , sacen-a4fc79 , sacen-a4fcc3 , sacen-a4fcq9 , sacen-a4fcs3 , sacen-a4fd56 , sacen-a4fdc0 , sacen-a4fdk1 , sacen-a4fdp9 , sacen-a4fdt6 , sacen-a4fdt8 , sacen-a4feu5 , sacen-a4few8 , sacen-a4ff15 , sacen-a4ffg0 , sacen-a4ffk3 , sacen-a4ffv6 , sacen-a4ffx1 , sacen-a4ffy1 , sacen-a4fgk3 , sacen-a4fgw0 , sacen-a4fh60 , sacen-a4fix9 , sacen-a4fiz9 , sacen-a4fja4 , sacen-a4fjd3 , sacen-a4fk07 , sacen-a4fk92 , sacen-a4fkg4 , sacen-a4fkn0 , sacen-a4flt0 , sacen-a4fmr1 , sacen-a4fmz0 , sacen-a4fn55 , sacen-a4fpb3 , sacen-a4fpf4 , sacen-a4fpp0 , sacen-a4fq24 , sacer-Q9JN61 , sacen-a4fnh7 , sacen-a4fd61 , sacen-a4ffa2

Title : The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin - Schwecke_1995_Proc.Natl.Acad.Sci.U.S.A_92_7839
Author(s) : Schwecke T , Aparicio JF , Molnar I , Konig A , Khaw LE , Haydock SF , Oliynyk M , Caffrey P , Cortes J , Lester JB , et al.
Ref : Proc Natl Acad Sci U S A , 92 :7839 , 1995
Abstract : The macrocyclic polyketides rapamycin and FK506 are potent immunosuppressants that prevent T-cell proliferation through specific binding to intracellular protein receptors (immunophilins). The cloning and specific alteration of the biosynthetic genes for these polyketides might allow the biosynthesis of clinically valuable analogues. We report here that three clustered polyketide synthase genes responsible for rapamycin biosynthesis in Streptomyces hygroscopicus together encode 14 homologous sets of enzyme activities (modules), each catalyzing a specific round of chain elongation. An adjacent gene encodes a pipecolate-incorporating enzyme, which completes the macrocycle. The total of 70 constituent active sites makes this the most complex multienzyme system identified so far. The DNA region sequenced (107.3 kbp) contains 24 additional open reading frames, some of which code for proteins governing other key steps in rapamycin biosynthesis.
ESTHER : Schwecke_1995_Proc.Natl.Acad.Sci.U.S.A_92_7839
PubMedSearch : Schwecke_1995_Proc.Natl.Acad.Sci.U.S.A_92_7839
PubMedID: 7644502
Gene_locus related to this paper: strhy-ORFZ