Liu XL

References (9)

Title : Cloning and rational modification of a cold-adapted esterase for phthalate esters and parabens degradation - Liu_2023_Chemosphere_325_138393
Author(s) : Liu YY , Zhang YX , Wen HM , Liu XL , Fan XJ
Ref : Chemosphere , 325 :138393 , 2023
Abstract : Phthalate esters (PAEs) and parabens are environmental pollutants that can be toxic to human health. Herein, a cold-adapted esterase from the Mao-tofu metagenome named Est1260 was screened for its PAE-hydrolyzing potential in cold temperatures. The results showed that purified Est1260 could degrade a variety of PAEs and parabens at temperatures as low as 0 degreesC. After careful analysis of the structural information and molecular docking, site-saturation mutation was conducted at the identified hotspots. Protein expression of variant A1B6 doubled, and its thermal stability significantly improved (24 times) without sacrificing activity at low temperatures. In addition, Est1260 and its variants were activated by NaCl and demonstrated resistance to high concentrations of saline (up to 5 M), making it a potential biocatalyst for bioremediation of PAE and paraben-polluted environments.
ESTHER : Liu_2023_Chemosphere_325_138393
PubMedSearch : Liu_2023_Chemosphere_325_138393
PubMedID: 36925017

Title : Multiple biochemical indices and metabolomics of Clonorchis sinensis provide a novel interpretation of biomarkers - Qiu_2022_Parasit.Vectors_15_172
Author(s) : Qiu YY , Chang QC , Gao JF , Bao MJ , Luo HT , Song JH , Hong SJ , Mao RF , Sun YY , Chen YY , Liu MY , Wang CR , Liu XL
Ref : Parasit Vectors , 15 :172 , 2022
Abstract : BACKGROUND: Clonorchiasis, an infectious disease caused by the liver fluke Clonorchis sinensis, may lead to the development of liver and gallbladder diseases, and even cholangiocarcinoma (CCA). However, the pathogenesis, host-pathogen interaction, and diagnostic markers for clonorchiasis remain unclear. METHODS: Eighteen rabbits were randomly divided into control group (n = 9) and C. sinensis-infected group (n = 9), and their plasma samples were collected at 7, 14, 28, and 63 days post-infection (dpi). Biochemical indices and metabolites in different infection periods were detected. A non-targeted ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach was employed to investigate the metabolic profiles of plasma in rabbits, and related metabolic pathways of differential metabolites and correlation between candidate biochemical indices and differential metabolites were analyzed. Finally, the candidate biomarkers were verified with human samples using a targeted metabolomics method. RESULTS: The result of biochemical indices indicated C. sinensis infection would affect the liver function biochemical indices, especially alanine aminotransferase, aspartate transaminase (AST), glutamyl transpeptidase (GGT), total bile acid, high-density lipoprotein, and cholinesterase. The metabonomic results showed that 58, 212, 23, and 21 differential metabolites were identified in different phases of the infection. Multivariate statistical analysis of differential metabolites revealed distinct metabolic signatures during different phases of infection, with most of these signatures being observed at 14 dpi, which mainly influences the amino acid metabolisms. For metabolites and biochemical indices, AST, GGT, hypoxanthine, L-pipecolic acid, and D-glucuronate represented potential noninvasive biomarkers for the diagnosis of C. sinensis (P < 0.05 and AUC > 0.8). Furthermore, GGT and D-glucuronate levels were positively correlated with the infection (r(28) = 0.98, P < 0.0001) and showed excellent diagnostic performance (AUC = 0.972; 95% confidence interval, 0.921 to 1.000). CONCLUSIONS: The present results provide new insights into plasma metabolic changes in rabbits during C. sinensis infection, and the potential biomarker may be used for developing an effective method to diagnose clonorchiasis in the future.
ESTHER : Qiu_2022_Parasit.Vectors_15_172
PubMedSearch : Qiu_2022_Parasit.Vectors_15_172
PubMedID: 35590378

Title : Enhanced hydrolysis of beta-cypermethrin caused by deletions in the glycin-rich region of carboxylesterase 001G from Helicoverpa armigera - Bai_2021_Pest.Manag.Sci_77_2129
Author(s) : Bai LS , Xu JJ , Zhao CX , Chang YL , Dong YL , Zhang KG , Li YQ , Li YP , Ma ZQ , Liu XL
Ref : Pest Manag Sci , 77 :2129 , 2021
Abstract : BACKGROUND: Carboxylesterase (CarE) is a major class of enzyme involved in the detoxification of toxic xenobiotics in various insect species. Previous work has shown that the carboxylesterase gene CarE001G found in Helicoverpa armigera is more active and can metabolize synthesized pyrethroids, such as beta-cypermethrin, one of the commonly used commercial insecticides for lepidopteran pest control. In addition, CarE001G is very special as it has a very specific glycine-rich region located adjacent to its C-terminal. But whether mutations in this unique sequence can change the biochemistry and function of CarE001G are unknown. RESULTS: In this study, four variants of CarE001G with different deletions in the glycine-rich region were obtained and functionally expressed in Escherichia coli. The recombinant proteins were purified and confirmed by Western blot and mass spectrometry analyses. These mutant enzymes showed high catalytic efficiency toward the model substrate alpha-naphthyl acetate. Inhibition study showed that beta-cypermethrin had relatively strong inhibition on CarE activities. In vitro metabolism assay showed that the mutant enzymes significantly enhanced their metabolic activities toward beta-cypermethrin with specific activities between 4.0 and 5.6 nmol L(-1) min(-1) mg(-1) protein. Molecular docking analyses consistently demonstrated that deletion mutations in the glycine-rich region may facilitate the anchoring of the beta-cypermethrin molecule in the active binding pocket of the mutant enzymes. CONCLUSION: The data show that deletion mutations can cause qualitative change in the capacity of CarEs in the detoxification of beta-cypermethrin. This indicates that deletion mutations in the glycine-rich region may have the potential to cause synthesized pyrethroid (SP) resistance in H. armigera in the future. 2020 Society of Chemical Industry.
ESTHER : Bai_2021_Pest.Manag.Sci_77_2129
PubMedSearch : Bai_2021_Pest.Manag.Sci_77_2129
PubMedID: 33336552
Gene_locus related to this paper: helam-d5g3d4

Title : Two single mutations in carboxylesterase 001C improve fenvalerate hydrolase activity in Helicoverpa armigera - Xu_2021_Pestic.Biochem.Physiol_179_104969
Author(s) : Xu JJ , Chang YM , Lu M , Tie Y , Dong YL , Chen GY , Ma ZQ , Liu XL , Li YQ
Ref : Pestic Biochem Physiol , 179 :104969 , 2021
Abstract : Carboxylesterases (CarEs) usually play critical roles in the detoxification of toxic chemicals and therefore may be involved in insecticide resistance in agricultural pests. Previous work has shown that CarE 001C from Helicoverpa armigera was able to metabolize the isomers of cypermethrin and fenvalerate. In this study, seven mutants of CarE 001C with single amino acid substitution were produced and expressed in the Escherichia coli. Enzyme kinetic analysis indicated that all seven mutations dramatically reduced enzymatic activities toward the generic substrate alpha-naphthyl acetate, but in vitro metabolism assay showed that two of the mutations, H423I and R322L, significantly improved hydrolase activities toward fenvalerate, with their recorded specific activities being 3.5 and 5.1 nM.s(-1).mg (-1) proteins, respectively. Further, thermostability assay showed that the stability of one mutant enzyme was enhanced. This study will help us better understand the potential of CarEs in insecticide detoxification and resistance in H. armigera.
ESTHER : Xu_2021_Pestic.Biochem.Physiol_179_104969
PubMedSearch : Xu_2021_Pestic.Biochem.Physiol_179_104969
PubMedID: 34802519
Gene_locus related to this paper: helam-d5kx87

Title : Functional Characterization of two Carboxylesterase Genes Involved in Pyrethroid Detoxification in Helicoverpa armigera - Li_2020_J.Agric.Food.Chem_68_3390
Author(s) : Li Y , Bai L , Zhao C , Xu J , Sun Z , Dong Y , Li D , Liu XL , Ma ZQ
Ref : Journal of Agricultural and Food Chemistry , 68 :3390 , 2020
Abstract : Insect carboxylesterases are major enzymes involved in metabolism of xenobiotics including insecticides. Two carboxylesterase genes, CarE001A and CarE001H, were cloned from the destructive agricultural pest Helicoverpa armigera. Quantitative Real-Time PCR showed that CarE001A and CarE001H were predominantly expressed in fat body and midgut, respectively; developmental expression analyses found that the expression levels of both CarEs were significantly higher in fifth- instar larvae than in other life stages. Recombinant CarE001A and CarE001H expressed in the Escherichia coli exhibited high enzymatic activity toward alpha-naphthyl acetate. Inhibition assays showed that organophosphates had strong inhibition on CarEs activity compared to pyrethroids. Metabolism assays indicated that CarE001A and CarE001H were able to metabolize beta-cypermethrin and lambda-cyhalothrin. Homology modeling and molecular docking analyses demonstrated that beta-cypermethrin could fit nicely into the active pocket of both carboxylesterases. These results suggested that CarE001A and CarE001H could play important roles in the detoxification of pyrehtroids in H. armigera.
ESTHER : Li_2020_J.Agric.Food.Chem_68_3390
PubMedSearch : Li_2020_J.Agric.Food.Chem_68_3390
PubMedID: 32096985
Gene_locus related to this paper: helam-d5g3d5 , helam-d9iv61

Title : Three-dimensional visualization of the functional fascicular groups of a long-segment peripheral nerve - Qi_2018_Neural.Regen.Res_13_1465
Author(s) : Qi J , Wang WY , Zhong YC , Zhou JM , Luo P , Tang P , He CF , Zhu S , Liu XL , Zhang Y
Ref : Neural Regen Res , 13 :1465 , 2018
Abstract : The three-dimensional (3D) visualization of the functional bundles in the peripheral nerve provides direct and detailed intraneural spatial information. It is useful for selecting suitable surgical methods to repair nerve defects and in optimizing the construction of tissue-engineered nerve grafts. However, there remain major technical hurdles in obtaining, registering and interpreting 2D images, as well as in establishing 3D models. Moreover, the 3D models are plagued by poor accuracy and lack of detail and cannot completely reflect the stereoscopic microstructure inside the nerve. To explore and help resolve these key technical problems of 3D reconstruction, in the present study, we designed a novel method based on re-imaging techniques and computer image layer processing technology. A 20-cm ulnar nerve segment from the upper arm of a fresh adult cadaver was used for acetylcholinesterase (AChE) staining. Then, 2D panoramic images were obtained before and after AChE staining under the stereomicroscope. Using layer processing techniques in Photoshop, a space transformation method was used to fulfill automatic registration. The contours were outlined, and the 3D rendering of functional fascicular groups in the long-segment ulnar nerve was performed with Amira 4.1 software. The re-imaging technique based on layer processing in Photoshop produced an image that was detailed and accurate. The merging of images was accurate, and the whole procedure was simple and fast. The least square support vector machine was accurate, with an error rate of only 8.25%. The 3D reconstruction directly revealed changes in the fusion of different nerve functional fascicular groups. IN CONCLUSION: The technique is fast with satisfactory visual reconstruction.
ESTHER : Qi_2018_Neural.Regen.Res_13_1465
PubMedSearch : Qi_2018_Neural.Regen.Res_13_1465
PubMedID: 30106060

Title : Discovery of a SNP in exon 7 of the lipoprotein lipase gene and its association with fatness traits in native and Cherry Valley Peking ducks - Wu_2008_Anim.Genet_39_564
Author(s) : Wu Y , Zhang HL , Wang J , Liu XL
Ref : Anim Genet , 39 :564 , 2008
Abstract : Lipoprotein lipase (LPL) is a multifunctional protein, playing a major role in the hydrolysis of triglycerides in chylomicrons and very low-density lipoproteins. This study was designed to investigate the effects of variants in exon 7 of the LPL gene on duck growth and fatness traits. Two populations (native and Cherry Valley Peking duck populations) were used in this study. Growth and fatness traits were measured in them. Primers for exon 7 of the LPL gene were designed from chicken genomic and cDNA sequences. Polymorphisms were detected by DNA sequencing, and a PCR-SSCP method was developed to genotype the two populations. The results showed one novel polymorphism: a synonymous alteration in exon 7 of the LPL gene (c.91C>T; EU834120 and EU834121). It was associated with abdominal fat weight and percentage of abdominal fat weight of native Peking duck and with percentage of subcutaneous fat plus skin weight and abdominal fat weight of Cherry Valley Peking duck. The alteration in exon 7 of LPL may be linked with potential major loci or genes affecting some fatness traits.
ESTHER : Wu_2008_Anim.Genet_39_564
PubMedSearch : Wu_2008_Anim.Genet_39_564
PubMedID: 18671687

Title : [Histochemical study of intrinsic laryngeal muscles in neonates]. [Chinese] - Liu_1993_Zhonghua.Er.Bi.Yan.Hou.Ke.Za.Zhi_28_278
Author(s) : Liu XL
Ref : Chung Hua Erh Pi Yen Hou Ko Tsa Chih Chinese Journal of Otorhinolaryngology , 28 :278 , 1993
Abstract : The motor end-plates and muscle fiber types in muscles of cricothyroid, posterior cricoarytenoid, thyroarytenoid were in five neonates and two adults studied by staining with acetylcholinesterase (AChE) and succinic dehydrogenase (SDH). The muscle fibers in neonate could be classified into three types: red, white and intermediate. The diameters of muscle fibers were different, with the white muscle fiber widest and the red ones narrowest. Each of the laryngeal muscles was composed of three muscle types in proportion. The motor end-plate showed a concentrated distribution band.
ESTHER : Liu_1993_Zhonghua.Er.Bi.Yan.Hou.Ke.Za.Zhi_28_278
PubMedSearch : Liu_1993_Zhonghua.Er.Bi.Yan.Hou.Ke.Za.Zhi_28_278
PubMedID: 8192929

Title : The histochemical study of rat sciatic nerve cholinesterase in degeneration and regeneration - Liu_1990_J.Reconstr.Microsurg_6_43
Author(s) : Liu XL , Zhu JK
Ref : J Reconstr Microsurg , 6 :43 , 1990
Abstract : With a modified, less time-consuming, Karnovsky-Roots method, this study evaluated the reduction and recovery of rat sciatic nerve cholinesterase (ChE) in degeneration and regeneration. Characteristics of motor fascicles included not only partial staining of acetylcholinesterase (AchE) in the axon, but also a small amount of more intense staining of butyrylcholinesterase (BuchE) in the unmyelinated fibers, scattered between the myelinated fibers in a spot or fleck form. Characteristics of sensory fascicles included more intense staining of BuchE in the unmyelinated fibers, (appearing as irregular balls or pieces), and a scattering between unstained myelinated fibers. Mixed fascicles had characteristics of both motor and sensory fascicles. Unmyelinated fibers exhibited BuchE activity, but myelinated fibers exhibited AchE activity. Preparation time for a specimen to react to ChE-positive staining was about 1 to 2 hr; the method is very suitable for diagnosis during surgery. After nerve transection, AchE activity at the distal nerve end began to reduce gradually; no AchE could be tested after two weeks. But BuchE activity in unmyelinated fibers could be found until 30 days after transection. After epineurial suturing of the peripheral nerve transection, new AchE activity could be found at the anastomosis site until about two weeks and at 1 cm distal to the anastomosis site until about three weeks. It became more intense with the passage of time and, at about six weeks, regenerated AchE-positive myelinated fibers could be seen at the distal end of the nerve.
ESTHER : Liu_1990_J.Reconstr.Microsurg_6_43
PubMedSearch : Liu_1990_J.Reconstr.Microsurg_6_43
PubMedID: 2308127