Chen YY

References (13)

Title : Neuroprotection by tetramethylpyrazine and its synthesized analogues for central nervous system diseases: a review - Feng_2024_Mol.Biol.Rep_51_159
Author(s) : Feng F , Xu DQ , Yue SJ , Chen YY , Tang YP
Ref : Mol Biol Rep , 51 :159 , 2024
Abstract : BACKGROUND: Due to the global increase in aging populations and changes in modern lifestyles, the prevalence of neurodegenerative diseases, cerebrovascular disorders, neuropsychiatrcic conditions, and related ailments is rising, placing an increasing burden on the global public health system. MATERIALS AND METHODS: All studies on tetramethylpyrazine (TMP) and its derivatives were obtained from reputable sources such as PubMed, Elsevier, Library Genesis, and Google Scholar. Comprehensive data on TMP and its derivatives was meticulously compiled. RESULTS: This comprehensive analysis explains the neuroprotective effects demonstrated by TMP and its derivatives in diseases of the central nervous system. These compounds exert their influence on various targets and signaling pathways, playing crucial roles in the development of various central nervous system diseases. Their multifaceted mechanisms include inhibiting oxidative damage, inflammation, cell apoptosis, calcium overload, glutamate excitotoxicity, and acetylcholinesterase activity. CONCLUSION: This review provides a brief summary of the most recent advancements in research on TMP and its derivatives in the context of central nervous system diseases. It involves synthesizing analogs of TMP and evaluating their effectiveness in models of central nervous system diseases. The ultimate goal is to facilitate the practical application of TMP and its derivatives in the future treatment of central nervous system diseases.
ESTHER : Feng_2024_Mol.Biol.Rep_51_159
PubMedSearch : Feng_2024_Mol.Biol.Rep_51_159
PubMedID: 38252346

Title : Preparation and characterization of immobilized 5-HT(1A) receptor stationary phase for high throughput screening of the receptor-binding ligands from complex systems like Curcuma wenyujin Y. H. Chen et C. Ling extract - Chen_2022_J.Pharm.Biomed.Anal_211_114632
Author(s) : Chen YY , Jin YH , Shayiranbieke A , Zhao X , Fan HS , Li Q , Zhao XF
Ref : J Pharm Biomed Anal , 211 :114632 , 2022
Abstract : The incidence of depression has increased significantly during the COVID-19 pandemic. This disease is closely associated with serotonin (1A) (5-HT(1A)) receptor and often treated by complex prescription containing Curcuma wenyujin Y. H. Chen et C. Ling. Therefore, we hypothesized that this herb contains bioactive compounds specially binding to the receptor. However, the rapid discovery of new ligands of 5-HT(1A) receptor is still challenging due to the lack of efficient screening methods. To address this problem, we developed and characterized a novel approach for the rapid screening of ligands by using immobilized 5-HT(1A) receptor as the chromatographic stationary phase. Briefly, haloalkane dehalogenase was fused at the C-terminal of 5-HT(1A) receptor, and the modified 5-HT(1A) receptor was immobilized on amino-microspheres by the reaction between haloalkane dehalogenase and 6-chlorohexanoic acid linker. Scanning electron microscope and X-ray photo-electron were used to characterize the morphology and element of the immobilized receptor. The binding of three specific ligands to 5-HT(1A) receptor was investigated by two different methods. Moreover, we examined the feasibility of 5-HT(1A) receptor colume in high throughput screening of new ligands from complex systems as exemplified by Curcuma wenyujin Y. H. Chen et C. Ling. Gweicurculactone, 2-hydroxy-1-(3,4-dihydroxybenzene)-7-(4'-hydroxybezene)-heptane and curcuminol F were identified as the ligands of 5-HT(1A) receptor with the binding energies of -7.06 kcal/mol, -7.77 kcal/mol and -5.26 kcal/mol, respectively. Collectively, these results indicated that the immobilized 5-HT(1A) receptor was capable of screening bioactive compound from complex system, providing an effective methodology for high throughput screening.
ESTHER : Chen_2022_J.Pharm.Biomed.Anal_211_114632
PubMedSearch : Chen_2022_J.Pharm.Biomed.Anal_211_114632
PubMedID: 35131672

Title : Multiple biochemical indices and metabolomics of Clonorchis sinensis provide a novel interpretation of biomarkers - Qiu_2022_Parasit.Vectors_15_172
Author(s) : Qiu YY , Chang QC , Gao JF , Bao MJ , Luo HT , Song JH , Hong SJ , Mao RF , Sun YY , Chen YY , Liu MY , Wang CR , Liu XL
Ref : Parasit Vectors , 15 :172 , 2022
Abstract : BACKGROUND: Clonorchiasis, an infectious disease caused by the liver fluke Clonorchis sinensis, may lead to the development of liver and gallbladder diseases, and even cholangiocarcinoma (CCA). However, the pathogenesis, host-pathogen interaction, and diagnostic markers for clonorchiasis remain unclear. METHODS: Eighteen rabbits were randomly divided into control group (n = 9) and C. sinensis-infected group (n = 9), and their plasma samples were collected at 7, 14, 28, and 63 days post-infection (dpi). Biochemical indices and metabolites in different infection periods were detected. A non-targeted ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach was employed to investigate the metabolic profiles of plasma in rabbits, and related metabolic pathways of differential metabolites and correlation between candidate biochemical indices and differential metabolites were analyzed. Finally, the candidate biomarkers were verified with human samples using a targeted metabolomics method. RESULTS: The result of biochemical indices indicated C. sinensis infection would affect the liver function biochemical indices, especially alanine aminotransferase, aspartate transaminase (AST), glutamyl transpeptidase (GGT), total bile acid, high-density lipoprotein, and cholinesterase. The metabonomic results showed that 58, 212, 23, and 21 differential metabolites were identified in different phases of the infection. Multivariate statistical analysis of differential metabolites revealed distinct metabolic signatures during different phases of infection, with most of these signatures being observed at 14 dpi, which mainly influences the amino acid metabolisms. For metabolites and biochemical indices, AST, GGT, hypoxanthine, L-pipecolic acid, and D-glucuronate represented potential noninvasive biomarkers for the diagnosis of C. sinensis (P < 0.05 and AUC > 0.8). Furthermore, GGT and D-glucuronate levels were positively correlated with the infection (r(28) = 0.98, P < 0.0001) and showed excellent diagnostic performance (AUC = 0.972; 95% confidence interval, 0.921 to 1.000). CONCLUSIONS: The present results provide new insights into plasma metabolic changes in rabbits during C. sinensis infection, and the potential biomarker may be used for developing an effective method to diagnose clonorchiasis in the future.
ESTHER : Qiu_2022_Parasit.Vectors_15_172
PubMedSearch : Qiu_2022_Parasit.Vectors_15_172
PubMedID: 35590378

Title : Blockade of soluble epoxide hydrolase attenuates post-ischemic neuronal hyperexcitation and confers resilience against stroke with TrkB activation - Chang_2018_Sci.Rep_8_118
Author(s) : Chang LH , Lin HC , Huang SS , Chen IC , Chu KW , Chih CL , Liang YW , Lee YC , Chen YY , Lee YH , Lee IH
Ref : Sci Rep , 8 :118 , 2018
Abstract : Inhibition and deletion of soluble epoxide hydrolase (sEH) has been suggested to ameliorate infarction in experimental ischemic stroke possibly via vasoactive epoxyeicosatrienoic acids. However, it is unknown whether the neuroprotective mechanisms involve alteration of post-ischemic neuronal transmission and neurotrophic signaling. We used a permanent middle cerebral artery occlusion (MCAO) model in adult wild-type mice with the sEH inhibitor 12-(3-adamantan-1-yl-ureido)dodecanoic acid (AUDA) post-treatment and in sEH knockout (sEH KO) mice. We found that sensorimotor recovery was significantly enhanced after MCAO in both AUDA-treated and sEH KO mice, with decreased sEH activity and brain infarction. Decreased post-ischemic long-term potentiation (iLTP) was observed in an ex vivo hippocampal oxygen-glucose deprivation model. Tropomyosin receptor kinase B (TrkB) activation, rather than glutamate receptor alteration, was consistently found after the different manipulations. Immunohistochemistry further revealed peri-infarct neuronal TrkB activation and microvasculature augmentation in AUDA-treated and sEH KO mice, suggesting parallel neurovascular enhancement. Mechanistically, pretreatment with a selective TrkB antagonist ANA12 countered the effect of iLTP attenuation induced by sEH deletion ex vivo and abolished the infarct reduction in vivo. Together, the neuroprotective effects of sEH inhibition and gene deletion can both be mediated partially via enhancement of TrkB signaling which attenuated post-ischemic neuroexcitation and neurological deficits.
ESTHER : Chang_2018_Sci.Rep_8_118
PubMedSearch : Chang_2018_Sci.Rep_8_118
PubMedID: 29311641

Title : The Apostasia genome and the evolution of orchids - Zhang_2017_Nature_549_379
Author(s) : Zhang GQ , Liu KW , Li Z , Lohaus R , Hsiao YY , Niu SC , Wang JY , Lin YC , Xu Q , Chen LJ , Yoshida K , Fujiwara S , Wang ZW , Zhang YQ , Mitsuda N , Wang M , Liu GH , Pecoraro L , Huang HX , Xiao XJ , Lin M , Wu XY , Wu WL , Chen YY , Chang SB , Sakamoto S , Ohme-Takagi M , Yagi M , Zeng SJ , Shen CY , Yeh CM , Luo YB , Tsai WC , Van de Peer Y , Liu ZJ
Ref : Nature , 549 :379 , 2017
Abstract : Constituting approximately 10% of flowering plant species, orchids (Orchidaceae) display unique flower morphologies, possess an extraordinary diversity in lifestyle, and have successfully colonized almost every habitat on Earth. Here we report the draft genome sequence of Apostasia shenzhenica, a representative of one of two genera that form a sister lineage to the rest of the Orchidaceae, providing a reference for inferring the genome content and structure of the most recent common ancestor of all extant orchids and improving our understanding of their origins and evolution. In addition, we present transcriptome data for representatives of Vanilloideae, Cypripedioideae and Orchidoideae, and novel third-generation genome data for two species of Epidendroideae, covering all five orchid subfamilies. A. shenzhenica shows clear evidence of a whole-genome duplication, which is shared by all orchids and occurred shortly before their divergence. Comparisons between A. shenzhenica and other orchids and angiosperms also permitted the reconstruction of an ancestral orchid gene toolkit. We identify new gene families, gene family expansions and contractions, and changes within MADS-box gene classes, which control a diverse suite of developmental processes, during orchid evolution. This study sheds new light on the genetic mechanisms underpinning key orchid innovations, including the development of the labellum and gynostemium, pollinia, and seeds without endosperm, as well as the evolution of epiphytism; reveals relationships between the Orchidaceae subfamilies; and helps clarify the evolutionary history of orchids within the angiosperms.
ESTHER : Zhang_2017_Nature_549_379
PubMedSearch : Zhang_2017_Nature_549_379
PubMedID: 28902843
Gene_locus related to this paper: 9aspa-a0a2i0b2l6 , 9aspa-a0a2i0w093 , 9aspa-a0a2i0asr1 , 9aspa-a0a2i0vyy1 , 9aspa-a0a2i0a218 , 9aspa-a0a2i0x5j6 , 9aspa-a0a2i0aji0 , 9aspa-a0a2i0a3k8 , 9aspa-a0a2i0win6 , 9aspa-a0a2i0vg82 , 9aspa-a0a2h9zyy3

Title : The Dendrobium catenatum Lindl. genome sequence provides insights into polysaccharide synthase, floral development and adaptive evolution - Zhang_2016_Sci.Rep_6_19029
Author(s) : Zhang GQ , Xu Q , Bian C , Tsai WC , Yeh CM , Liu KW , Yoshida K , Zhang LS , Chang SB , Chen F , Shi Y , Su YY , Zhang YQ , Chen LJ , Yin Y , Lin M , Huang H , Deng H , Wang ZW , Zhu SL , Zhao X , Deng C , Niu SC , Huang J , Wang M , Liu GH , Yang HJ , Xiao XJ , Hsiao YY , Wu WL , Chen YY , Mitsuda N , Ohme-Takagi M , Luo YB , Van de Peer Y , Liu ZJ
Ref : Sci Rep , 6 :19029 , 2016
Abstract : Orchids make up about 10% of all seed plant species, have great economical value, and are of specific scientific interest because of their renowned flowers and ecological adaptations. Here, we report the first draft genome sequence of a lithophytic orchid, Dendrobium catenatum. We predict 28,910 protein-coding genes, and find evidence of a whole genome duplication shared with Phalaenopsis. We observed the expansion of many resistance-related genes, suggesting a powerful immune system responsible for adaptation to a wide range of ecological niches. We also discovered extensive duplication of genes involved in glucomannan synthase activities, likely related to the synthesis of medicinal polysaccharides. Expansion of MADS-box gene clades ANR1, StMADS11, and MIKC(*), involved in the regulation of development and growth, suggests that these expansions are associated with the astonishing diversity of plant architecture in the genus Dendrobium. On the contrary, members of the type I MADS box gene family are missing, which might explain the loss of the endospermous seed. The findings reported here will be important for future studies into polysaccharide synthesis, adaptations to diverse environments and flower architecture of Orchidaceae.
ESTHER : Zhang_2016_Sci.Rep_6_19029
PubMedSearch : Zhang_2016_Sci.Rep_6_19029
PubMedID: 26754549
Gene_locus related to this paper: 9aspa-a0a2i0w093 , 9aspa-a0a2i0vyy1 , 9aspa-a0a2i0x5j6 , 9aspa-a0a2i0win6 , 9aspa-a0a2i0vg82

Title : Armillaridin, a Honey Medicinal Mushroom, Armillaria mellea (Higher Basidiomycetes) Component, Inhibits Differentiation and Activation of Human Macrophages - Liu_2015_Int.J.Med.Mushrooms_17_161
Author(s) : Liu TP , Chen CC , Shiao PY , Shieh HR , Chen YY , Chen YJ
Ref : Int J Med Mushrooms , 17 :161 , 2015
Abstract : Armillaridin (AM) is an aromatic ester compound isolated from honey medicinal mushroom, Armillaria mellea, which has anti-cancer potential. This study was designed to examine the effects of AM on differentiation and activation macrophages, the major ontogeny of innate immunity. Macrophages were derived from CD14+ monocytes which were sorted from human peripheral blood mononuclear cells. Cell viability was assessed by trypan blue exclusion test. Cells were stained with Liu's dye for observation of morphology. Expression of surface antigens was examined by flow cytometric analysis. Phagocytosis and generation of reactive oxygen species (ROS), as functional assays, were evaluated by counting engulfed yeasts and DCFH-DA reaction. The viability of macrophages was not significantly reduced by AM. AM at nontoxic concentrations markedly increased cytoplasmic vacuoles. The expression of surface CD14, CD16, CD36, and HLA-DR was suppressed. The phagocytosis function, but not ROS production, of macrophages was inhibited by AM. Armillaridin could inhibit the differentiation and activation of human macrophages. It may have potential to be developed as a biological response modifier for inflammatory diseases.
ESTHER : Liu_2015_Int.J.Med.Mushrooms_17_161
PubMedSearch : Liu_2015_Int.J.Med.Mushrooms_17_161
PubMedID: 25746621
Gene_locus related to this paper: armos-armb

Title : Differential expression profile of membrane proteins in Aplysia pleural-pedal ganglia under the stress of methyl parathion - Chen_2014_Environ.Sci.Pollut.Res.Int_21_3371
Author(s) : Chen YY , Huang L , Zhang Y , Ke CH , Huang HQ
Ref : Environ Sci Pollut Res Int , 21 :3371 , 2014
Abstract : This study was aimed to analyze the alteration of membrane protein profiles in Aplysia juliana Quoy & Gaimard (A. juliana) pleural-pedal ganglia under MP exposure. Both the results of GC-MS analysis and the activity assay of acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT) reveal that MP toxicological effects on Aplysia left and right pleural-pedal ganglia are different under 7 and 14 days of exposure. Therefore, Aplysia were subjected for exposure at two concentrations (1 and 2 mg/l) of MP for 7 and 14 days for membrane proteomic study. As a result, 19 and 14 protein spots were differentially expressed in A. juliana left pleural-pedal ganglia under 7 and 14 days treatment, and 20 and 14 protein spots found with differential expressions in their right ganglia under the same treatment, respectively. Several proteins with expression variations were detected from both the left and right pleural-pedal ganglia; however, most proteins have distinctive expressions, indicating different mechanisms might be involved in initiating MP toxicology in left and right ganglia. Among the total differential protein spots obtained, 29 proteins were classed as membrane proteins. These proteins are mainly involved in the metabolism process, cell redox homeostasis, signal transduction, immunology, intracellular transport and catalysis, indicating MP toxicity in mollusks seems to be complex and diverse. Some differentially expressed proteins were further confirmed by Western blotting and quantitative real-time PCR. These results might provide renovated insights to reveal the mechanism of MP-induced neurotoxicity, and the novel candidate biomarkers might have potential application for environmental evaluation of MP pollution level.
ESTHER : Chen_2014_Environ.Sci.Pollut.Res.Int_21_3371
PubMedSearch : Chen_2014_Environ.Sci.Pollut.Res.Int_21_3371
PubMedID: 24234813

Title : Antibacterial bisabolane-type sesquiterpenoids from the sponge-derived fungus Aspergillus sp - Li_2012_Mar.Drugs_10_234
Author(s) : Li D , Xu Y , Shao CL , Yang RY , Zheng CJ , Chen YY , Fu XM , Qian PY , She ZG , de Voogd NJ , Wang CY
Ref : Mar Drugs , 10 :234 , 2012
Abstract : Four new bisabolane-type sesquiterpenoids, aspergiterpenoid A (1), (-)-sydonol (2), (-)-sydonic acid (3), and (-)-5-(hydroxymethyl)-2-(2',6',6'-trimethyltetrahydro-2H- pyran-2-yl)phenol (4) together with one known fungal metabolite (5) were isolated from the fermentation broth of a marine-derived fungus Aspergillus sp., which was isolated from the sponge Xestospongia testudinaria collected from the South China Sea. Four of them (1-4) are optically active compounds. Their structures and absolute configurations were elucidated by using NMR spectroscopic techniques and mass spectrometric analysis, and by comparing their optical rotations with those related known analogues. Compounds 1-5 showed selective antibacterial activity against eight bacterial strains with the MIC (minimum inhibiting concentrations) values between 1.25 and 20.0 microM. The cytotoxic, antifouling, and acetylcholinesterase inhibitory activities of these compounds were also examined.
ESTHER : Li_2012_Mar.Drugs_10_234
PubMedSearch : Li_2012_Mar.Drugs_10_234
PubMedID: 22363233

Title : Complete genome and transcriptomes of Streptococcus parasanguinis FW213: phylogenic relations and potential virulence mechanisms - Geng_2012_PLoS.One_7_e34769
Author(s) : Geng J , Chiu CH , Tang P , Chen Y , Shieh HR , Hu S , Chen YY
Ref : PLoS ONE , 7 :e34769 , 2012
Abstract : Streptococcus parasanguinis, a primary colonizer of the tooth surface, is also an opportunistic pathogen for subacute endocarditis. The complete genome of strain FW213 was determined using the traditional shotgun sequencing approach and further refined by the transcriptomes of cells in early exponential and early stationary growth phases in this study. The transcriptomes also discovered 10 transcripts encoding known hypothetical proteins, one pseudogene, five transcripts matched to the Rfam and additional 87 putative small RNAs within the intergenic regions defined by the GLIMMER analysis. The genome contains five acquired genomic islands (GIs) encoding proteins which potentially contribute to the overall pathogenic capacity and fitness of this microbe. The differential expression of the GIs and various open reading frames outside the GIs at the two growth phases suggested that FW213 possess a range of mechanisms to avoid host immune clearance, to colonize host tissues, to survive within oral biofilms and to overcome various environmental insults. Furthermore, the comparative genome analysis of five S. parasanguinis strains indicates that albeit S. parasanguinis strains are highly conserved, variations in the genome content exist. These variations may reflect differences in pathogenic potential between the strains.
ESTHER : Geng_2012_PLoS.One_7_e34769
PubMedSearch : Geng_2012_PLoS.One_7_e34769
PubMedID: 22529932

Title : Complete genome sequence of the ureolytic Streptococcus salivarius strain 57.I - Geng_2011_J.Bacteriol_193_5596
Author(s) : Geng J , Huang SC , Li S , Hu S , Chen YY
Ref : Journal of Bacteriology , 193 :5596 , 2011
Abstract : Streptococcus salivarius 57.I is one of the most abundant and highly ureolytic bacteria in the human mouth. It can utilize urea as the sole nitrogen source via the activity of urease. Complete genome sequencing of S. salivarius 57.I revealed a chromosome and a phage which are absent in strain SK126.
ESTHER : Geng_2011_J.Bacteriol_193_5596
PubMedSearch : Geng_2011_J.Bacteriol_193_5596
PubMedID: 21914897

Title : Diethyl 2,6-dimethyl-4-phenyl-1,4-dihydro-pyridine-3,5-dicarboxyl-ate - Bai_2009_Acta.Crystallogr.Sect.E.Struct.Rep.Online_65_o799
Author(s) : Bai MS , Chen YY , Niu DL , Peng L
Ref : Acta Crystallographica Sect E Struct Rep Online , 65 :o799 , 2009
Abstract : The title mol-ecule, C(19)H(23)NO(4), was synthesized by the reaction of benzaldehyde, ethyl acetoacetate and NH(4)HCO(3). The dihydro-pyridine ring adopts a flattened boat conformation and the plane of the base of the boat forms a dihedral angle of 88.78 (9) degrees with the phenyl ring. The packing is stabilized by strong inter-molecular N-Hcdots, three dots, centeredO and weak inter-molecular C-Hcdots, three dots, centeredO hydrogen bonds.
ESTHER : Bai_2009_Acta.Crystallogr.Sect.E.Struct.Rep.Online_65_o799
PubMedSearch : Bai_2009_Acta.Crystallogr.Sect.E.Struct.Rep.Online_65_o799
PubMedID: 21582523

Title : Lipase and pancreatic amylase activities in diagnosis of acute pancreatitis in patients with hyperamylasemia - Yang_2005_Hepatobiliary.Pancreat.Dis.Int_4_600
Author(s) : Yang RW , Shao ZX , Chen YY , Yin Z , Wang WJ
Ref : Hepatobiliary Pancreat Dis Int , 4 :600 , 2005
Abstract : BACKGROUND: Measurement of total serum amylase (AMY) is the most widely used biochemical test for the diagnosis of acute pancreatitis, but it is commonly considered a nonspecific marker. To improve the biochemical diagnosis of acute pancreatitis, lipase (LIP) and pancreatic amylase (PAMY) have been tested in recent years. The present study was designed to evaluate whether serum LIP and pancreatic PAMY tests could replace total amylase test to improve diagnostic efficiency in the evaluation of acute pancreatitis in patients with hyperamylasemia.
METHODS: LIP and PAMY values were determined in serum samples from 92 patients with hyperamylasemia. Reference values for each enzyme were derived from serum samples of 147 healthy subjects. The activities of LIP and PAMY in patients with various diseases were shown directly by the boxplot graph. The diagnostic accuracy of LIP and PAMY was defined as the area under the receiver operating characteristic (ROC) curve. Their sensitivity and specificity in detecting acute pancreatitis at varying cutoff points were shown by the curve, and the best cutoff value for each enzyme was shown by the modified ROC curve. The diagnostic values of LIP, PAMY and LIP+AMY with each upper limit of reference range (ULR) were compared with the corresponding best cutoff values.
RESULTS: The references values of LIP and PAMY were 12.2-47.6 U/L and 28-95 U/L, respectively. These values in patients with acute pancreatitis were higher than those patients with other diseases. The areas under the ROC curve (AUC) of LIP and PAMY were 0.799 and 0.792, respectively. With the best diagnostic cutoff point of maximum (sensitivity + specificity)-100%, we obtained values of 97.9 U/L (LIP(97.9)=2.06 X ULR) for LIP and 209 U/L (PAMY(209)=2.20 X ULR) for PAMY. The best cutoff values for LIP, PAMY and LIP+AMY demonstrated the specificity, positive predictive value, and diagnostic efficiency higher than the corresponding ULRs.
CONCLUSIONS: Serum LIP and PAMY are specific for the pancreas and might replace total amylase for the diagnosis of acute pancreatitis in hyperamylasemia patients. LIP(97.9) is more efficient than PAMY(209) in the diagnosis of acute pancreatitis. A combined test of both enzymes is not superior to single test of either enzyme in diagnostic accuracy.
ESTHER : Yang_2005_Hepatobiliary.Pancreat.Dis.Int_4_600
PubMedSearch : Yang_2005_Hepatobiliary.Pancreat.Dis.Int_4_600
PubMedID: 16286272