Mikami LR


Full name : Mikami Liya R

First name : Liya R

Mail : Department of Genetics, Federal University of Parana, Curitiba, Parana

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Country : Brazil

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References (6)

Title : Association between RAPH1 Gene Haplotypes and CHE2 Locus Phenotypes - De Andrade_2016_Ann.Hum.Genet_80_203
Author(s) : de Andrade FA , Batistela MS , Amaral Sda C , Dos Santos W , Mikami LR , Chautard-Freire-Maia EA , Furtado-Alle L , de Souza RLR
Ref : Ann Hum Genet , 80 :203 , 2016
Abstract : The human butyrylcholinesterase (BChE) is a serum esterase that has been associated with body mass index (BMI) and obesity. Its activity is conditioned by alleles of BCHE gene and the CHE2 locus that codifies an unknown BChE-binding protein (C5 complex). The hypothesis that the CHE2 locus is the RAPH1 gene, which encodes lamellipodin (Lpd), was raised in a study that observed Lpd peptides released from denatured BChE tetramers. The aim of this study was to test this hypothesis by evaluating SNPs of RAPH1 gene (rs2246118:C > T, rs3814365:A > G and rs2465520:C > T) in 34 CHE2 C5+ and 92 CHE2 C5- individuals, corresponding to the presence and absence of C5 complex. The results showed association of two haplotypes (CAC and TGC) with CHE2 C5+ phenotype. RAPH1 haplotypes was also associated with intense (TGC) and faint (CAC) CHE2 C5+ phenotypes. BChE activity was higher in intense CHE2 C5+ than faint CHE2 C5+ phenotype. Our results corroborate the hypothesis that the RAPH1 gene is the CHE2 locus and suggest that the variable expressivity of the CHE2 C5+ phenotypes is, at least in part, due to its genetic heterogeneity, which is leading to increased BChE activity only in individuals with intense CHE2 C5+ phenotype.
ESTHER : De Andrade_2016_Ann.Hum.Genet_80_203
PubMedSearch : De Andrade_2016_Ann.Hum.Genet_80_203
PubMedID: 27346732

Title : Two new mutations of the human BCHE gene (IVS3-14T>C and L574fsX576) - Parmo-Folloni_2008_Chem.Biol.Interact_175_135
Author(s) : Parmo-Folloni F , Nunes K , Lepienski LM , Mikami LR , de Souza RLR , Tsuneto LT , Petzl-Erler ML , Chautard-Freire-Maia EA
Ref : Chemico-Biological Interactions , 175 :135 , 2008
Abstract : The genetic variation of human butyrylcholinesterase is associated with the majority of prolonged cases of apnea in patients submitted to the muscle relaxant succinylcholine. The present study reports two new mutations of the BCHE gene in 346 Euro-Brazilians: IVS3-14T>C found in five heterozygotes (allele frequency: 0.72+/-0.32%) and L574fsX576 found in one heterozygote (allele frequency: 0.14+/-0.14%). These two variants were not found in 85 Guarani Amerindians. It is not expected that the IVS3-14T>C mutation may interfere in the splicing process and that the mutation found in exon 4 (L574fsX576) may disturb BChE tetramerization and activity.
ESTHER : Parmo-Folloni_2008_Chem.Biol.Interact_175_135
PubMedSearch : Parmo-Folloni_2008_Chem.Biol.Interact_175_135
PubMedID: 18555211

Title : Five new naturally occurring mutations of the BCHE gene and frequencies of 12 butyrylcholinesterase alleles in a Brazilian population - Mikami_2008_Pharmacogenet.Genomics_18_213
Author(s) : Mikami LR , Wieseler S , de Souza RLR , Schopfer LM , Nachon F , Lockridge O , Chautard-Freire-Maia EA
Ref : Pharmacogenet Genomics , 18 :213 , 2008
Abstract : Human butyrylcholinesterase (BChE; EC is codified by the BCHE gene (3q26.1-q26.2) in which 65 variants have been identified. BChE is a scavenger of organophosphorus and carbamate compounds and hydrolyzes succinylcholine, mivacurium and cocaine. The present study describes 12 naturally occurring BCHE mutations including five new mutations (K12R, G15G, V294M, G333C and R470W) identified in 366 blood donors from Southern Brazil. Exons 2 and 4 of the BCHE gene were examined by PCR-SSCA and samples with unexpected electrophoretic patterns were sequenced. The respective nucleotide substitution that characterizes each of the four new nonsynonymous mutations was introduced into BCHE cDNA by site directed mutagenesis and transfected into human embryonic kidney 293T cells and/or Chinese hamster ovary cells. The catalyzed hydrolysis of butyrylthiocholine (BTC) by BChE was measured by the Ellman method. Enzyme kinetic parameters obtained after the expression of the respective recombinant BChE evaluated the effects of the four nonsynonymous mutations. Thirty-four out of 366 individuals carried a BChE mutation in exon 2. The K variant mutation, A539T in exon 4, was present in one out of three persons. Gene expression showed that only one of the newly identified mutations (G333C) altered BChE activity, leading to a decrease of about 80% in relation to the wild-type enzyme.
ESTHER : Mikami_2008_Pharmacogenet.Genomics_18_213
PubMedSearch : Mikami_2008_Pharmacogenet.Genomics_18_213
PubMedID: 18300943

Title : Association of variants of the -116 site of the butyrylcholinesterase BCHE gene to enzyme activity and body mass index - Furtado-Alle_2008_Chem.Biol.Interact_175_115
Author(s) : Furtado-Alle L , Andrade FA , Nunes K , Mikami LR , de Souza RLR , Chautard-Freire-Maia EA
Ref : Chemico-Biological Interactions , 175 :115 , 2008
Abstract : Butyrylcholinesterase (BChE) is coded by the BCHE gene that presents four exons. The non-codifying exon 1 presents two variants -116G and -116A, being -116A preferentially in cis conformation with the 539T variant (K) of exon 4 which was associated with lower BChE activity and lower body mass index (BMI) variance. This study analyzed the frequency of -116 variants and the relation of genotypes -116GG;539AA, -116GG;539AT and -116GA;539AT with BChE activity and with BMI in Euro-Brazilian blood donors. The frequency of -116A was significantly higher (18.9%) in the low BChE activity group when compared to obese (8.6%) and normal BMI (9.3%) groups. In obese and non-obese groups, the -116GA;539AT genotype showed significantly lower mean BChE activity when compared to the -116GG;539AA genotype and in obese individuals the -116GA;539AT genotype also showed lower BChE activity than the -116GG;539AT genotype. In a sample selected independently of BMI, the -116GA;539AT genotype showed significantly higher BMI variance (21.75) when compared to -116GG;539AA (12.14) and to -116GG;539AT (13.43) genotypes, indicating that the association with higher BMI variance only occurs in the presence of the -116A variant. In the obese sample, the -116GG;539AT genotype presented mean (32.1+/-0.3) and variance (2.3) of BMI significantly lower than those found in the -116GG;539AA (33.0+/-0.3 and 9.9, respectively) and -116GA;539AT (33.7+/-0.7 and 12.2, respectively) genotypes. These data show that: (1) the K (539T) variant alone is not associated with decreased BChE activity, being the 5' UTR -116A variant necessary for this decrease, probably by affecting transcription and/or translation of the BCHE gene; (2) samples with different BMI distributions present different relationships between BCHE genotypes and BMI, reinforcing the hypothesis of a role for the BCHE gene in BMI determination.
ESTHER : Furtado-Alle_2008_Chem.Biol.Interact_175_115
PubMedSearch : Furtado-Alle_2008_Chem.Biol.Interact_175_115
PubMedID: 18550040

Title : Expression of three naturally occurring genetic variants (G75R, E90D, I99M) of the BCHE gene of human butyrylcholinesterase - Mikami_2007_Pharmacogenet.Genomics_17_681
Author(s) : Mikami LR , Wieseler S , de Souza RLR , Schopfer LM , Lockridge O , Chautard-Freire-Maia EA
Ref : Pharmacogenet Genomics , 17 :681 , 2007
Abstract : The present paper examined the effects of three non synonymous BCHE mutations (G75R, E90D and /99M) on enzyme kinetic parameters obtained after the expression of the respective recombinant BChEs. The respective nucleotide substitution that characterizes each of the three variants was introduced into BCHE cDNA by site directed mutagenesis and transfected into human embryonic kidney 293 T cells and Chinese hamster ovary cells (for E90D). BChE catalysed hydrolysis of butyrylthiocoline (BTC) was measured by Ellman method. The expression results showed that: (1) the activity of the G75R enzyme represents approximately 45% of the wild-type activity, whereas that of the I99M enzyme does not differ from the wild-type; (2) the E90D enzyme presents a silent phenotype; disruption of the salt bridge between E90 and R42 may cause the enzyme to be rapidly degraded inside the cells. In homozygous form the E90D enzyme may confer increased susceptibility to succinylcholine, but may delay cognitive impairment in aged individuals. BChE genotyping may become important for estimating prognosis, and the knowledge of the genetic variants of BChE in a particular population may be useful for carrying out the genotyping assays.
ESTHER : Mikami_2007_Pharmacogenet.Genomics_17_681
PubMedSearch : Mikami_2007_Pharmacogenet.Genomics_17_681
PubMedID: 17700357

Title : Four new mutations in the BCHE gene of human butyrylcholinesterase in a Brazilian blood donor sample - de Souza_2005_Mol.Genet.Metab_84_349
Author(s) : de Souza RLR , Mikami LR , Maegawa RO , Chautard-Freire-Maia EA
Ref : Mol Genet Metab , 84 :349 , 2005
Abstract : The genetic variation of human butyrylcholinesterase has been associated with height, body mass index, Alzheimer's disease, and response to xenobiotic agents. The present study reports four new mutations, found in the exon 2 of the BCHE gene, in a sample from 3001 Brazilian blood donors. The three nonsynonymous mutations and one synonymous mutation detected are: 223G-->C, G75R; 270A-->C, E90 D; 297T-->G, I99 M; 486T-->C, A162 A, respectively. All these variants are rare: 0.093+/-0.093% for the missense mutations and 0.137+/-0.137% for the synonymous mutation. A table with the 58 non-usual variants of butyrylcholinesterase is also presented.
ESTHER : de Souza_2005_Mol.Genet.Metab_84_349
PubMedSearch : de Souza_2005_Mol.Genet.Metab_84_349
PubMedID: 15781196