Szaniszlo PJ

References (4)

Title : Comparative genomic and transcriptomic analysis of wangiella dermatitidis, a major cause of phaeohyphomycosis and a model black yeast human pathogen - Chen_2014_G3.(Bethesda)_4_561
Author(s) : Chen Z , Martinez DA , Gujja S , Sykes SM , Zeng Q , Szaniszlo PJ , Wang Z , Cuomo CA
Ref : G3 (Bethesda) , 4 :561 , 2014
Abstract : Black or dark brown (phaeoid) fungi cause cutaneous, subcutaneous, and systemic infections in humans. Black fungi thrive in stressful conditions such as intense light, high radiation, and very low pH. Wangiella (Exophiala) dermatitidis is arguably the most studied phaeoid fungal pathogen of humans. Here, we report our comparative analysis of the genome of W. dermatitidis and the transcriptional response to low pH stress. This revealed that W. dermatitidis has lost the ability to synthesize alpha-glucan, a cell wall compound many pathogenic fungi use to evade the host immune system. In contrast, W. dermatitidis contains a similar profile of chitin synthase genes as related fungi and strongly induces genes involved in cell wall synthesis in response to pH stress. The large portfolio of transporters may provide W. dermatitidis with an enhanced ability to remove harmful products as well as to survive on diverse nutrient sources. The genome encodes three independent pathways for producing melanin, an ability linked to pathogenesis; these are active during pH stress, potentially to produce a barrier to accumulated oxidative damage that might occur under stress conditions. In addition, a full set of fungal light-sensing genes is present, including as part of a carotenoid biosynthesis gene cluster. Finally, we identify a two-gene cluster involved in nucleotide sugar metabolism conserved with a subset of fungi and characterize a horizontal transfer event of this cluster between fungi and algal viruses. This work reveals how W. dermatitidis has adapted to stress and survives in diverse environments, including during human infections.
ESTHER : Chen_2014_G3.(Bethesda)_4_561
PubMedSearch : Chen_2014_G3.(Bethesda)_4_561
PubMedID: 24496724
Gene_locus related to this paper: exodn-h6bmp3 , exodn-h6btr2 , exodn-h6c4y3

Title : Characterization of two polyketide synthase genes in Exophiala lecanii-corni, a melanized fungus with bioremediation potential - Cheng_2004_Bioorg.Chem_32_92
Author(s) : Cheng Q , Kinney KA , Whitman CP , Szaniszlo PJ
Ref : Bioorg Chem , 32 :92 , 2004
Abstract : Exophiala lecanii-corni has significant bioremediation potential because it can degrade a wide range of volatile organic compounds. In order to identify sites for the insertion of genes that might enhance this potential, a genetic analysis of E. lecanii-corni was undertaken. Two polyketide synthase genes, ElPKS1 and ElPKS2, have now been discovered by a PCR-based strategy. ElPKS1 was isolated by a marker rescue technique. The nucleotide sequence of ElPKS1 consists of a 6576-bp open reading frame encoding a protein with 2192 amino acids, which was interrupted by a 60-bp intron near the 5' end and a 54-bp intron near the 3' end. Sequence analysis, results from disruption experiments, and physiological tests showed that ElPKS1 encoded a polyketide synthase required for melanin biosynthesis. Since ElPKS1 is non-essential, it is a desirable bioengineering target site for the insertion of native and foreign genes. The successful expression of these genes could enhance the bioremediation capability of the organism. ElPKS2 was cloned by colony hybridization screening of a partial genomic library with an ElPKS2 PCR product. ElPKS2 had a 6465-bp open reading frame that encoded 2155 amino acids and had introns of 56, 67, 54, and 71 bp. Although sequence analysis of the derived protein of ElPKS2 confirmed the polyketide synthase nature of its protein product, the function of that product remains unclear.
ESTHER : Cheng_2004_Bioorg.Chem_32_92
PubMedSearch : Cheng_2004_Bioorg.Chem_32_92
PubMedID: 14990308
Gene_locus related to this paper: 9pezi-q7z9k6 , exole-PKS2

Title : Molecular Cloning and Characterization of WdPKS1, a Gene Involved in Dihydroxynaphthalene Melanin Biosynthesis and Virulence in Wangiella (Exophiala) dermatitidis. -
Author(s) : Feng B , Wang X , Hauser M , Kaufmann S , Jentsch S , Haase G , Becker JM , Szaniszlo PJ
Ref : Infect Immun , 69 :1781 , 2001
PubMedID: 11179356
Gene_locus related to this paper: exode-PKS1

Title : A color-selectable and site-specific integrative transformation system for gene expression studies in the dematiaceous fungus Wangiella (Exophiala) dermatitidis - Ye_1999_Curr.Genet_36_241
Author(s) : Ye X , Feng B , Szaniszlo PJ
Ref : Curr Genet , 36 :241 , 1999
Abstract : To explore potential virulence factors in the dematiaceous (melanized) fungus Wangiella dermatitidis, we established a gene expression system with properties of homologous transformation and color identification. Using a polyketide synthase gene (WdPKS1) fragment for targeting, we found that 52% of transformants became albinos easily distinguishable from nonspecific transformants. Southern analysis confirmed that the integrations were at the WdPKS1 locus, which however did not affect transformant growth. With a heterologous promoter, P-glaA, enhanced expression of lacZ was found at 37 degrees C. Our results indicated that this system allows the efficient production of isogenic strains for gene function analysis in W. dermatitidis.
ESTHER : Ye_1999_Curr.Genet_36_241
PubMedSearch : Ye_1999_Curr.Genet_36_241
PubMedID: 10541862
Gene_locus related to this paper: exode-PKS1