Ye X

References (23)

Title : Molecular simulations guide immobilization of lipase on nest-like ZIFs with regulatable hydrophilic\/hydrophobic surface - Zhong_2024_J.Colloid.Interface.Sci_667_199
Author(s) : Zhong L , Wang Z , Ye X , Cui J , Jia S
Ref : J Colloid Interface Sci , 667 :199 , 2024
Abstract : The catalytic performance of immobilized lipase is greatly influenced by functional support, which attracts growing interest for designing supports to achieve their promotive catalytic activity. Many lipases bind strongly to hydrophobic surfaces where they undergo interfacial activation. Herein, the behavioral differences of lipases with distinct lid structures on interfaces of varying hydrophobicity levels were firstly investigated by molecular simulations. It was found that a reasonable hydrophilic/hydrophobic surface could facilitate the lipase to undergo interfacial activation. Building on these findings, a novel "nest"-like superhydrophobic ZIFs (ZIFN) composed of hydrophobic ligands was prepared for the first time and used to immobilize lipase from Aspergillus oryzae (AOL@ZIFN). The AOL@ZIFN exhibited 2.0-folds higher activity than free lipase in the hydrolysis of p-Nitrophenyl palmitate (p-NPP). Especially, the modification of superhydrophobic ZIFN with an appropriate amount of hydrophilic tannic acid can significantly improve the activity of the immobilized lipase (AOL@ZIFN-TA). The AOL@ZIFN-TA exhibited 30-folds higher activity than free lipase, and still maintained 82% of its initial activity after 5 consecutive cycles, indicating good reusability. These results demonstrated that nanomaterials with rational arrangement of the hydrophilic/hydrophobic surface could facilitate the lipase to undergo interfacial activation and improve its activity, displaying the potential of the extensive application.
ESTHER : Zhong_2024_J.Colloid.Interface.Sci_667_199
PubMedSearch : Zhong_2024_J.Colloid.Interface.Sci_667_199
PubMedID: 38636222

Title : Personalized venlafaxine dose prediction using artificial intelligence technology: a retrospective analysis based on real-world data - Liu_2024_Int.J.Clin.Pharm__
Author(s) : Liu Y , Yu Z , Ye X , Zhang J , Hao X , Gao F , Yu J , Zhou C
Ref : Int J Clin Pharm , : , 2024
Abstract : BACKGROUND: Venlafaxine dose regimens vary considerably between individuals, requiring personalized dosing. AIM: This study aimed to identify dose-related influencing factors of venlafaxine through real-world data analysis and to construct a personalized dose model using advanced artificial intelligence techniques. METHOD: We conducted a retrospective study on patients with depression treated with venlafaxine. Significant variables were selected through a univariate analysis. Subsequently, the predictive performance of seven models (XGBoost, LightGBM, CatBoost, GBDT, ANN, TabNet, and DT) was compared. The algorithm that demonstrated optimal performance was chosen to establish the dose prediction model. Model validation used confusion matrices and ROC analysis. Additionally, a dose subgroup analysis was conducted. RESULTS: A total of 298 patients were included. TabNet was selected to establish the venlafaxine dose prediction model, which exhibited the highest performance with an accuracy of 0.80. The analysis identified seven crucial variables correlated with venlafaxine daily dose, including blood venlafaxine concentration, total protein, lymphocytes, age, globulin, cholinesterase, and blood platelet count. The area under the curve (AUC) for predicting venlafaxine doses of 75 mg, 150 mg, and 225 mg were 0.90, 0.85, and 0.90, respectively. CONCLUSION: We successfully developed a TabNet model to predict venlafaxine doses using real-world data. This model demonstrated substantial predictive accuracy, offering a personalized dosing regimen for venlafaxine. These findings provide valuable guidance for the clinical use of the drug.
ESTHER : Liu_2024_Int.J.Clin.Pharm__
PubMedSearch : Liu_2024_Int.J.Clin.Pharm__
PubMedID: 38733475

Title : Biotransformation of HBCDs by the microbial communities enriched from mangrove sediments - Yu_2024_J.Hazard.Mater_469_134036
Author(s) : Yu F , Zhang B , Liu Y , Luo W , Chen H , Gao J , Ye X , Li J , Xie Q , Peng T , Wang H , Huang T , Hu Z
Ref : J Hazard Mater , 469 :134036 , 2024
Abstract : 1,2,5,6,9,10-Hexabromocyclododecanes (HBCDs) are a sort of persistent organic pollutants (POPs). This research investigated 12 microbial communities enriched from sediments of four mangroves in China to transform HBCDs. Six microbial communities gained high transformation rates (27.5-97.7%) after 12 generations of serial transfer. Bacteria were the main contributors to transform HBCDs rather than fungi. Analyses on the bacterial compositions and binning genomes showed that Alcanivorax (55.246-84.942%) harboring haloalkane dehalogenase genes dadAH and dadBH dominated the microbial communities with high transformation rates. Moreover, expressions of dadAH and dadBH in the microbial communities and Alcanivorax isolate could be induced by HBCDs. Further, it was found that purified proteins DadAH and DadBH showed high conversion rates on HBCDs in 36 h (91.9 +/- 7.4 and 101.0 +/- 1.8%, respectively). The engineered Escherichia coli BL21 strains harbored two genes could convert 5.7 +/- 0.4 and 35.1 +/- 0.1% HBCDs, respectively, lower than their cell-free crude extracts (61.2 +/- 5.2 and 56.5 +/- 8.7%, respectively). The diastereoisomer-specific transforming trend by both microbial communities and enzymes were gamma- > alpha- > beta-HBCD, differed from alpha- > beta- > gamma-HBCD by the Alcanivorax isolate. The identified transformation products indicated that HBCDs were dehalogenated via HBr elimination (dehydrobromination), hydrolytic and reductive debromination pathways in the enriched cultures. Two enzymes converted HBCDs via hydrolytic debromination. The present research provided theoretical bases for the biotransformation of HBCDs by microbial community and the bioremediation of HBCDs contamination in the environment.
ESTHER : Yu_2024_J.Hazard.Mater_469_134036
PubMedSearch : Yu_2024_J.Hazard.Mater_469_134036
PubMedID: 38493623

Title : Serum cholinesterase is associated with incident diabetic retinopathy: the Shanghai Nicheng cohort study - Yu_2023_Nutr.Metab.(Lond)_20_26
Author(s) : Yu R , Ye X , Wang X , Wu Q , Jia L , Dong K , Zhu Z , Bao Y , Hou X , Jia W
Ref : Nutr Metab (Lond) , 20 :26 , 2023
Abstract : BACKGROUND: Serum cholinesterase (ChE) is positively associated with incident diabetes and dyslipidemia. We aimed to investigate the relationship between ChE and the incidence of diabetic retinopathy (DR). METHODS: Based on a community-based cohort study followed for 4.6 years, 1133 participants aged 55-70 years with diabetes were analyzed. Fundus photographs were taken for each eye at both baseline and follow-up investigations. The presence and severity of DR were categorized into no DR, mild non-proliferative DR (NPDR), and referable DR (moderate NPDR or worse). Binary and multinomial logistic regression models were used to estimate the risk ratio (RR) and 95% confidence interval (CI) between ChE and DR. RESULTS: Among the 1133 participants, 72 (6.4%) cases of DR occurred. The multivariable binary logistic regression showed that the highest tertile of ChE (<= 422 U/L) was associated with a 2.01-fold higher risk of incident DR (RR 2.01, 95%CI 1.01-4.00; P for trend < 0.05) than the lowest tertile (< 354 U/L). The multivariable binary and multinomial logistic regression showed that the risk of DR increased by 41% (RR 1.41, 95%CI 1.05-1.90), and the risk of incident referable DR was almost 2-fold higher than no DR (RR 1.99, 95%CI 1.24-3.18) with per 1-SD increase of log(e)-transformed ChE. Furthermore, multiplicative interactions were found between ChE and elderly participants (aged 60 and older; P for interaction = 0.003) and men (P for interaction = 0.044) on the risk of DR. CONCLUSIONS: In this study, ChE was associated with the incidence of DR, especially referable DR. ChE was a potential biomarker for predicting the incident DR.
ESTHER : Yu_2023_Nutr.Metab.(Lond)_20_26
PubMedSearch : Yu_2023_Nutr.Metab.(Lond)_20_26
PubMedID: 37138337

Title : Branched poly(ethylenimine) carbon dots-MnO(2) nanosheets based fluorescent sensory system for sensing of malachite green in fish samples - Mu_2022_Food.Chem_394_133517
Author(s) : Mu X , Liu X , Ye X , Zhang W , Li L , Ma P , Song D
Ref : Food Chem , 394 :133517 , 2022
Abstract : Malachite green (MG) is an organic dye compound that is frequently used as a fungicide and antiseptic in aquaculture. However, human or animal exposure to MG causes carcinogenic, teratogenic and mutagenic effects. Herein, a novel fluorescent assay was designed for the detection of MG using manganese dioxide nanosheets (MnO(2) NS) as an energy acceptor to quench the fluorescence of branched poly(ethylenimine) carbon dots (BPEI-CDs) via Forster resonance energy transfer. When butyrylcholinesterase is introduced to form thiocholine in the presence of S-butyrylthiocholine iodide, MnO(2) NS can be recovered by thiocholine to Mn(2+), resulting in restoration of the fluorescence of BPEI-CDs. Exploiting these changes in fluorescence intensity in the above system, a fluorescence probe was successfully developed for the quantitative detection of MG. Besides, this assay was applied to fish samples, verifying the high potential for practical application of the proposed sensor for the monitoring of MG in aquatic products.
ESTHER : Mu_2022_Food.Chem_394_133517
PubMedSearch : Mu_2022_Food.Chem_394_133517
PubMedID: 35749877

Title : The chromosome-level genome of Chinese praying mantis Tenodera sinensis (Mantodea: Mantidae) reveals its biology as a predator - Yuan_2022_Gigascience_12_
Author(s) : Yuan R , Zheng B , Li Z , Ma X , Shu X , Qu Q , Ye X , Li S , Tang P , Chen X
Ref : Gigascience , 12 : , 2022
Abstract : BACKGROUND: The Chinese praying mantis, Tenodera sinensis (Saussure), is a carnivorous insect that preys on a variety of arthropods and small vertebrates, including pest species. Several studies have been conducted to understand its behavior and physiology. However, there is limited knowledge about the genetic information underlying its genome evolution, digestive demands, and predatory behaviors. FINDINGS: Here we have assembled the chromosome-level genome of T. sinensis, representing the first sequenced genome of the family Mantidae, with a genome size of 2.54 Gb and scaffold N50 of 174.78 Mb. Our analyses revealed that 98.6% of BUSCO genes are present, resulting in a well-annotated assembly compared to other insect genomes, containing 25,022 genes. The reconstructed phylogenetic analysis showed the expected topology placing the praying mantis in an appropriate position. Analysis of transposon elements suggested the Gypsy/Dirs family, which belongs to long terminal repeat (LTR) transposons, may be a key factor resulting in the larger genome size. The genome shows expansions in several digestion and detoxification associated gene families, including trypsin and glycosyl hydrolase (GH) genes, ATP-binding cassette (ABC) transporter, and carboxylesterase (CarE), reflecting the possible genomic basis of digestive demands. Furthermore, we have found 1 ultraviolet-sensitive opsin and 2 long-wavelength-sensitive (LWS) opsins, emphasizing the core role of LWS opsins in regulating predatory behaviors. CONCLUSIONS: The high-quality genome assembly of the praying mantis provides a valuable repository for studying the evolutionary patterns of the mantis genomes and the gene expression profiles of insect predators.
ESTHER : Yuan_2022_Gigascience_12_
PubMedSearch : Yuan_2022_Gigascience_12_
PubMedID: 37882605

Title : Conformational selection in biocatalytic plastic degradation by PETase - Guo_2022_ACS.Catal_12_3397
Author(s) : Guo B , Vanga SR , Lopez-Lorenzo X , Saenz-Mendez P , Ericsson SR , Fang Y , Ye X , Schriever K , Backstrom E , Biundo A , Zubarev RA , Furo I , Hakkarainen M , Syren PO
Ref : ACS Catal , 12 :3397 , 2022
Abstract : Due to the steric effects imposed by bulky polymers, the formation of catalytically competent enzyme and substrate conformations is critical in the biodegradation of plastics. In poly(ethylene terephthalate) (PET), the backbone adopts different conformations, gauche and trans, coexisting to different extents in amorphous and crystalline regions. However, which conformation is susceptible to biodegradation and the extent of enzyme and substrate conformational changes required for expedient catalysis remain poorly understood. To overcome this obstacle, we utilized molecular dynamics simulations, docking, and enzyme engineering in concert with high-resolution microscopy imaging and solid-state nuclear magnetic resonance (NMR) to demonstrate the importance of conformational selection in biocatalytic plastic hydrolysis. Our results demonstrate how single-amino acid substitutions in Ideonella sakaiensis PETase can alter its conformational landscape, significantly affecting the relative abundance of productive ground-state structures ready to bind discrete substrate conformers. We experimentally show how an enzyme binds to plastic and provide a model for key residues involved in the recognition of gauche and trans conformations supported by in silico simulations. We demonstrate how enzyme engineering can be used to create a trans-selective variant, resulting in higher activity when combined with an all-trans PET-derived oligomeric substrate, stemming from both increased accessibility and conformational preference. Our work cements the importance of matching enzyme and substrate conformations in plastic hydrolysis, and we show that also the noncanonical trans conformation in PET is conducive for degradation. Understanding the contribution of enzyme and substrate conformations to biocatalytic plastic degradation could facilitate the generation of designer enzymes with increased performance.
ESTHER : Guo_2022_ACS.Catal_12_3397
PubMedSearch : Guo_2022_ACS.Catal_12_3397
PubMedID:

Title : Chemical Constituents and their Antioxidant, Anti-Inflammatory and Anti-Acetylcholinesterase Activities from Pholidota cantonensis - Liu_2021_Plant.Foods.Hum.Nutr_76_105
Author(s) : Liu L , Zou M , Zeng K , Ye X , Wang R , Wang W , Zhang X
Ref : Plant Foods Hum Nutr , 76 :105 , 2021
Abstract : Alzheimer's disease (AD) has the third highest health expenditures after heart disease and cancer. It has emerged as a serious global health issue. The discovery of new drugs to prevent and treat AD is of utmost importance. Pholidota cantonensis is an edible medicinal plant consumed in China. It is widely used in traditional Chinese medicine to treat various diseases. P. cantonensis has been reported to have antioxidant, anti-inflammatory, antitumor and antibacterial activities. Among these properties, its potent antioxidant activity has attracted our attention, since oxidative stress is one of the important pathological mechanisms involved in AD. This study aimed to isolate the compounds from the active extract and evaluate their bioactivities. Fifteen compounds, including one new compound, were obtained. The isolates were tested for 2,2'-diphenyl-1-picrylhydrazyl (DPPH)/2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities, anti-acetylcholinesterase (anti-AChE) activities and inhibitory effects on nitrogen monoxide (NO) release in the BV-2 cells. Compounds 1, 2, 4, 6, 8, and 13-15 exhibited two kinds of AD-associated bioactivities. More importantly, compound 13 showed more potent NO inhibitory activity (IC(50) = 0.72 +/- 0.08 microM) than the positive control quercetin (IC(50) = 12.94 +/- 0.08 microM). Compound 13 also had a higher inhibitory rate (99.59 +/- 0.43%) on AChE than that of the positive control galantamine (78.32 +/- 1.16%) at the concentrate of 50 microg/mL. Our studies provide new insights into this plant in terms of its potential in the development of new multi-target anti-Alzheimer's disease (anti-AD) drugs.
ESTHER : Liu_2021_Plant.Foods.Hum.Nutr_76_105
PubMedSearch : Liu_2021_Plant.Foods.Hum.Nutr_76_105
PubMedID: 33620638

Title : Antioxidant and pancreatic lipase inhibitory effects of flavonoids from different citrus peel extracts: An in vitro study - Huang_2020_Food.Chem_326_126785
Author(s) : Huang R , Zhang Y , Shen S , Zhi Z , Cheng H , Chen S , Ye X
Ref : Food Chem , 326 :126785 , 2020
Abstract : Obesity and oxidative damage are two important risk factors associated closely with metabolic syndrome. Utilization of functional food ingredients is considered as a feasible way to tackle these challenges. In the present study, eight representative species of citrus peel extracts (CPEs) were evaluated and compared for their flavonoid profiles, antioxidant activities, and pancreatic lipase (PL) inhibitory capacities and mechanisms. Results indicated that hesperidin, naringin, neohesperidin, narirutin and eriocitrin were the five major flavonoids in CPEs, among which hesperidin was the main active PL inhibitor. Moreover, hesperidin could interact with PL by hydrogen bonds and van der Waals forces, and the interaction would not obviously change the secondary structure of PL. Overall, ponkan peel extract, having the strongest overall antioxidant activity, the highest content of hesperidin and total phenolic compounds among all tested CPEs, is a promising natural ingredient to scavenge free radicals and manage obesity.
ESTHER : Huang_2020_Food.Chem_326_126785
PubMedSearch : Huang_2020_Food.Chem_326_126785
PubMedID: 32438224

Title : Identification of key residues of carboxylesterase PxEst-6 involved in pyrethroid metabolism in Plutella xylostella (L.) - Li_2020_J.Hazard.Mater_407_124612
Author(s) : Li Y , Sun H , Tian Z , Ye X , Li R , Li X , Zheng S , Liu J , Zhang Y
Ref : J Hazard Mater , 407 :124612 , 2020
Abstract : The long-term and excessive use of insecticides has led to severe environmental problems and the evolution of insecticide resistance in insects. Carboxylesterases (CarEs) are important detoxification enzymes conferring insecticide resistance on insects. Herein, the detoxification process of Plutella xylostella (L.) carboxylesterase 6 (PxEst-6), one representative P. xylostella carboxylesterase, is investigated with cypermethrin, bifenthrin, cyfluthrin and lambda-cyhalothrin. RT-qPCR shows that PxEst-6 is highly expressed in the midgut and cuticles of the third instar larvae. Exposure to pyrethroid insecticides resulted in PxEst-6 up-regulation in a short time. Metabolic assays indicate that PxEst-6 has the capacity to metabolize these pyrethroid insecticides. The combination of molecular docking, binding mode analyses and alanine mutations demonstrated that His451, Lys458 and Gln431 were key residues of PxEst-6 for metabolizing pyrethroids and the acetate groups derived from pyrethroids were key sites for being metabolized by PxEst-6. H451- and K458-derived hydrogen bond (H-bond) interactions with the pyrethroid acetate groups and the polar interactions with the pyrethroid acetate group provided by the Q431 sidechain were crucial to the pyrethroids' metabolism by PxEst-6. Our study contributes to revealing the reasons for pyrethroid resistance in P. xylostella, and provides a fundamental basis for the development of novel pyrethroid insecticides.
ESTHER : Li_2020_J.Hazard.Mater_407_124612
PubMedSearch : Li_2020_J.Hazard.Mater_407_124612
PubMedID: 33338816
Gene_locus related to this paper: pluxy-f1c4b8

Title : Characterization of an acidic pectin methylesterase from Paenibacillus xylanexedens and its application in fruit processing - Zhong_2020_Protein.Expr.Purif_179_105798
Author(s) : Zhong L , Wang X , Fan L , Ye X , Li Z , Cui Z , Huang Y
Ref : Protein Expr Purif , 179 :105798 , 2020
Abstract : A pectinase-producing bacterial isolate, identified as Paenibacillus xylanexedens SZ 29, was screened by using the soil dilution plate with citrus pectin and congo red. A pectin methylesterase gene (Pxpme) was cloned and expressed in Escherichia coli. The gene coded for a protein with 334 amino acids and a calculated molecular mass of 36.76 kDa. PxPME showed the highest identity of 32.4% with the characterized carbohydrate esterase family 8 pectin methylesterase from Daucus carota. The recombined PxPME showed a specific activity with 39.38 U/mg against citrus pectin with >65% methylesterification. The optimal pH and temperature for PxPME activity were 5.0 and 45 degreeC. Its K(m) and V(max) value were determined to be 1.43 mg/mL and 71.5 mol/mg.min, respectively. Moreover, PxPME could increase the firmness of pineapple cubes by 114% when combined with CaCl(2). The acidic and mesophilic properties make PxPME a potential candidate for application in the fruit processing.
ESTHER : Zhong_2020_Protein.Expr.Purif_179_105798
PubMedSearch : Zhong_2020_Protein.Expr.Purif_179_105798
PubMedID: 33232801

Title : Catalpol prevents denervated muscular atrophy related to the inhibition of autophagy and reduces BAX\/BCL2 ratio via mTOR pathway - Wang_2019_Drug.Des.Devel.Ther_13_243
Author(s) : Wang Y , Shao Y , Gao Y , Wan G , Wan D , Zhu H , Qiu Y , Ye X
Ref : Drug Des Devel Ther , 13 :243 , 2019
Abstract : Aim: To investigate the effects of catalpol on muscular atrophy induced by sciatic nerve crush injury (SNCI). Methods: Seventy male Kunming mice were randomized into five groups (n=10): model, sham, catalpol (Cat), rapamycin (Rapa), and catalpol+rapamycin (Rapa+Cat). The ratio of gastrocnemius muscle wet weight (right/left, R/L) between the operated leg (right) and the normal leg (left) was calculated, and acetylcholinesterase (AChE) immunohistochemistry assays were performed to observe the change of motor end plate (MEP), along with the sizes of denervated and innervated muscle fibers. The expression levels of LC3II, TUNEL, BAX/BCL-2, LC3II/LC3I and P62, Beclin1, mTOR, and p-mTOR (ser2448) proteins in muscle were examined by fluorescence immunohistochemistry or Western blotting. Results: Results show that catalpol improved the results of the grid walking tests by reducing the percentage of foot slips, which increased the gastrocnemius muscle wet weight (R/L), enhanced AChE expression at the MEP, and enlarged the section area of the muscle. The expression of LC3II and TUNEL was significantly inhibited by catalpol. The BAX/BCL-2 ratio was significantly increased in muscles of denervated and control groups. Lower LC3II/LC3I and BAX/BCL-2 ratios in denervated muscles were also detected after catalpol treatment. Conclusion: These results indicated that apoptosis and autophagy play a role in the regulation of denervation-induced muscle atrophy after SNCI, and catalpol alleviates muscle atrophy through the regulation of muscle apoptosis and autophagy via the mTOR signaling pathway.
ESTHER : Wang_2019_Drug.Des.Devel.Ther_13_243
PubMedSearch : Wang_2019_Drug.Des.Devel.Ther_13_243
PubMedID: 30643390

Title : Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways - Ye_2017_Sci.Rep_7_4985
Author(s) : Ye X , Yang L , Stanley D , Li F , Fang Q
Ref : Sci Rep , 7 :4985 , 2017
Abstract : Among its other biological roles, acetylcholinesterase (AChE, EC 3.1.1.7), encoded by two ace in most insects, catalyses the breakdown of acetylcholine, thereby terminating synaptic transmission. ace1 encodes the synaptic enzyme and ace2 has other essential actions in many insect species, such as Chilo suppressalis and Plutella xylostella. The silkworm, Bombyx mori, has been domesticated for more than two thousand years and its aces have no history of pesticide exposure. Here, we investigated the functional differences between two ace genes, BmAce1 and BmAce2, in the silkworm. qPCR analysis indicated that BmAce1 is highly expressed in muscle and BmAce2 is more ubiquitously expressed among tissues and enriched in the head. Both genes were separately suppressed using chemically synthesized siRNAs. The mRNA abundance of the two ace genes was significantly reduced to about 13% - 75% of the control levels after siRNA injection. The AChE activities were decreased to 32% to 85% of control levels. Silencing BmAce2 resulted in about 26% mortality, faster and higher than the 20% in the siBmAce1-treated group. Silencing BmAce1 impacted motor control and development to a greater extent than silencing BmAce2, although both treatment groups suffered motor disability, slowed development and reduced cocoons. Both genes have essential, differing biological significance.
ESTHER : Ye_2017_Sci.Rep_7_4985
PubMedSearch : Ye_2017_Sci.Rep_7_4985
PubMedID: 28694460
Gene_locus related to this paper: bommo-ACHE1 , bommo-ACHE2

Title : Contribution of upregulated dipeptidyl peptidase 9 (DPP9) in promoting tumoregenicity, metastasis and the prediction of poor prognosis in non-small cell lung cancer (NSCLC) - Tang_2017_Int.J.Cancer_140_1620
Author(s) : Tang Z , Li J , Shen Q , Feng J , Liu H , Wang W , Xu L , Shi G , Ye X , Ge M , Zhou X , Ni S
Ref : International Journal of Cancer , 140 :1620 , 2017
Abstract : Dipeptidyl peptidase 9 (DPP9) is encoded by DPP9, which belongs to the DPP4 gene family. Proteins encoded by these genes have unique peptidase and extra-enzymatic functions that have been linked to various diseases including cancers. Here, we describe the expression pattern and biological function of DPP9 in non-small-cell lung cancer (NSCLC). The repression of DPP9 expression by small interfering RNA inhibited cell proliferation, migration, and invasion. Moreover, we explored the role of DPP9 in regulating epithelial-mesenchymal transition (EMT). The epithelial markers E-cadherin and MUC1 were significantly increased, while mesenchymal markers vimentin and S100A4 were markedly decreased in DPP9 knockdown cells. The downregulation of DPP9 in the NSCLC cells induced the expression of apoptosis-associated proteins both in vitro and in vivo. We investigated the protein expression levels of DPP9 by tissue microarray immunohistochemical assay (TMA-IHC) (n = 217). Further we found mRNA expression levels of DPP9 in 30 pairs of clinical NSCLC tissues were significantly lower than in the adjacent non-cancerous tissues. Survival analysis showed that the overexpression of DPP9 was a significant independent factor for poor 5-year overall survival in patients with NSCLC (p = 0.003). Taken together, DPP9 expression correlates with poor overall survival in NSCLC.
ESTHER : Tang_2017_Int.J.Cancer_140_1620
PubMedSearch : Tang_2017_Int.J.Cancer_140_1620
PubMedID: 27943262

Title : Direct dorsal hippocampal-prelimbic cortex connections strengthen fear memories - Ye_2017_Nat.Neurosci_20_52
Author(s) : Ye X , Kapeller-Libermann D , Travaglia A , Inda MC , Alberini CM
Ref : Nat Neurosci , 20 :52 , 2017
Abstract : The ability to regulate the consolidation and strengthening of memories for threatening experiences is critical for mental health, and its dysregulation may lead to psychopathologies. Re-exposure to the context in which the threat was experienced can either increase or decrease fear response through distinct processes known, respectively, as reconsolidation or extinction. Using a context retrieval-dependent memory-enhancement model in rats, we report that memory strengthens through activation of direct projections from dorsal hippocampus to prelimbic (PL) cortex and activation of critical PL molecular mechanisms that are not required for extinction. Furthermore, while sustained PL brain-derived neurotrophic factor (BDNF) expression is required for memory consolidation, retrieval engages PL BDNF to regulate excitatory and inhibitory synaptic proteins neuroligin 1 and neuroligin 2, which promote memory strengthening while inhibiting extinction. Thus, context retrieval-mediated fear-memory enhancement results from a concerted action of mechanisms that strengthen memory through reconsolidation while suppressing extinction.
ESTHER : Ye_2017_Nat.Neurosci_20_52
PubMedSearch : Ye_2017_Nat.Neurosci_20_52
PubMedID: 27869801

Title : A novel cold-adapted and highly salt-tolerant esterase from Alkalibacterium sp. SL3 from the sediment of a soda lake - Wang_2016_Sci.Rep_6_19494
Author(s) : Wang G , Wang Q , Lin X , Ng TB , Yan R , Lin J , Ye X
Ref : Sci Rep , 6 :19494 , 2016
Abstract : A novel esterase gene (estSL3) was cloned from the Alkalibacterium sp. SL3, which was isolated from the sediment of soda lake Dabusu. The 636-bp full-length gene encodes a polypeptide of 211 amino acid residues that is closely related with putative GDSL family lipases from Alkalibacterium and Enterococcus. The gene was successfully expressed in E. coli, and the recombinant protein (rEstSL3) was purified to electrophoretic homogeneity and characterized. rEstSL3 exhibited the highest activity towards pNP-acetate and had no activity towards pNP-esters with acyl chains longer than C8. The enzyme was highly cold-adapted, showing an apparent temperature optimum of 30 degrees C and remaining approximately 70% of the activity at 0 degrees C. It was active and stable over the pH range from 7 to 10, and highly salt-tolerant up to 5 M NaCl. Moreover, rEstSL3 was strongly resistant to most tested metal ions, chemical reagents, detergents and organic solvents. Amino acid composition analysis indicated that EstSL3 had fewer proline residues, hydrogen bonds and salt bridges than mesophilic and thermophilic counterparts, but more acidic amino acids and less hydrophobic amino acids when compared with other salt-tolerant esterases. The cold active, salt-tolerant and chemical-resistant properties make it a promising enzyme for basic research and industrial applications.
ESTHER : Wang_2016_Sci.Rep_6_19494
PubMedSearch : Wang_2016_Sci.Rep_6_19494
PubMedID: 26915906

Title : Upregulation of Acetylcholinesterase Mediated by p53 Contributes to Cisplatin-Induced Apoptosis in Human Breast Cancer Cell - Ye_2015_J.Cancer_6_48
Author(s) : Ye X , Zhang C , Chen Y , Zhou T
Ref : J Cancer , 6 :48 , 2015
Abstract : BACKGROUND: The expression of acetylcholinesterase (AChE) could be induced during apoptosis in various cell types. And reduced AChE expression either by siRNA could prevent apoptosis. However, the detailed mechanisms underlying the AChE regulation are largely unknown in human breast cancer cell. MATERIAL AND
METHODS: MCF-7 cells were cultured and treated by cisplatin in the absence or presence of p53 siRNA.
RESULTS: In this study, the regulation of AChE expression during apoptosis induced by cisplatin, a current used anticancer drug, was investigated in human breast cancer cell line MCF-7. Exposure of MCF-7 cells to cisplatin resulted in apoptosis in a time- and concentration-dependent manner. Meanwhile, the upregulated AChE and p53 were also observed during apoptosis. Silencing interfering RNA directed against p53 blocked the expression of AChE. CONCLUSION: Taken together, these results suggested that AChE expression could be upregulated by the activation of p53 during apoptosis induced by cisplatin in MCF-7 cells.
ESTHER : Ye_2015_J.Cancer_6_48
PubMedSearch : Ye_2015_J.Cancer_6_48
PubMedID: 25553088

Title : Endogenous and Synthetic ABHD5 Ligands Regulate ABHD5-Perilipin Interactions and Lipolysis in Fat and Muscle - Sanders_2015_Cell.Metab_22_851
Author(s) : Sanders MA , Madoux F , Mladenovic L , Zhang H , Ye X , Angrish M , Mottillo EP , Caruso JA , Halvorsen G , Roush WR , Chase P , Hodder P , Granneman JG
Ref : Cell Metab , 22 :851 , 2015
Abstract : Fat and muscle lipolysis involves functional interactions of adipose triglyceride lipase (ATGL), alpha-beta hydrolase domain-containing protein 5 (ABHD5), and tissue-specific perilipins 1 and 5 (PLIN1 and PLIN5). ABHD5 potently activates ATGL, but this lipase-promoting activity is suppressed when ABHD5 is bound to PLIN proteins on lipid droplets. In adipocytes, protein kinase A (PKA) phosphorylation of PLIN1 rapidly releases ABHD5 to activate ATGL, but mechanisms for rapid regulation of PLIN5-ABHD5 interaction in muscle are unknown. Here, we identify synthetic ligands that release ABHD5 from PLIN1 or PLIN5 without PKA activation and rapidly activate adipocyte and muscle lipolysis. Molecular imaging and affinity probe labeling demonstrated that ABHD5 is directly targeted by these synthetic ligands and additionally revealed that ABHD5-PLIN interactions are regulated by endogenous ligands, including long-chain acyl-CoA. Our results reveal a new locus of lipolysis control and suggest ABHD5 ligands might be developed into novel therapeutics that directly promote fat catabolism.
ESTHER : Sanders_2015_Cell.Metab_22_851
PubMedSearch : Sanders_2015_Cell.Metab_22_851
PubMedID: 26411340
Gene_locus related to this paper: human-ABHD5 , mouse-abhd5

Title : Does preconception paternal exposure to a physiologically relevant level of bisphenol A alter spatial memory in an adult rat? - Fan_2013_Horm.Behav_64_598
Author(s) : Fan Y , Ding S , Ye X , Manyande A , He D , Zhao N , Yang H , Jin X , Liu J , Tian C , Xu S , Ying C
Ref : Horm Behav , 64 :598 , 2013
Abstract : Bisphenol A (BPA) is a ubiquitous environmental endocrine disrupting compound (EDC); public health concerns have been fueled by findings that maternal BPA exposure can change sex differences in the brain and in some behaviors. We investigated whether a physiologically relevant dose of BPA ingested by male rats before conception would affect spatial memory and hippocampal acetylcholinesterase (AchE) in their adult offspring. Twenty-two 60-day-old male rats (F0) received either a BPA diet (50mug/kg/day) or vehicle alone for 10weeks before being mated with non-exposed females. The paternal rats and their forty adult offspring's (F1) behaviors were then examined in the Morris Water Maze (MWM) and their AchE activities in the hippocampus were evaluated. BPA exposure led to spatial memory deficits along with decreased AchE activities in the hippocampus (p=0.01) in adult F0 rats. This paternal exposure also induced impairment in spatial memory acquisition in both sexes while retention only in females in F1 rats, as well as abolished sex differences in the hippocampus AchE. Overall, these data provide new evidence that paternal BPA exposure, at a "safe" dose, may induce transgenerational alterations in spatial memory in a sex-specific manner.
ESTHER : Fan_2013_Horm.Behav_64_598
PubMedSearch : Fan_2013_Horm.Behav_64_598
PubMedID: 24005185

Title : Transsynaptic coordination of presynaptic and postsynaptic modifications underlying enduring synaptic plasticity - Ye_2011_Neuron_70_379
Author(s) : Ye X , Carew TJ
Ref : Neuron , 70 :379 , 2011
Abstract : Neurexins and neuroligins are cell adhesion molecules that form transsynaptic interactions. In this issue of Neuron, Choi et al. report that neurexin-neuroligin signaling plays a critical role in functional and structural synaptic plasticity underlying memory formation in Aplysia.
ESTHER : Ye_2011_Neuron_70_379
PubMedSearch : Ye_2011_Neuron_70_379
PubMedID: 21555066

Title : Acetylcholinesterase biosensor design based on carbon nanotube-encapsulated polypyrrole and polyaniline copolymer for amperometric detection of organophosphates - Du_2010_Biosens.Bioelectron_25_2503
Author(s) : Du D , Ye X , Cai J , Liu J , Zhang A
Ref : Biosensors & Bioelectronics , 25 :2503 , 2010
Abstract : A simple method to immobilize acetylcholinesterase (AChE) on polypyrrole (PPy) and polyaniline (PANI) copolymer doped with multi-walled carbon nanotubes (MWCNTs) was proposed. The synthesized PAn-PPy-MWCNTs copolymer presented a porous and homogeneous morphology which provided an ideal size to entrap enzyme molecules. Due to the biocompatible microenvironment provided by the copolymer network, the obtained composite was devised for AChE attachment, resulting in a stable AChE biosensor for screening of organophosphates (OPs) exposure. MWCNTs promoted electron-transfer reactions at a lower potential and catalyzed the electro-oxidation of thiocholine, thus increasing detection sensitivity. Based on the inhibition of OPs on the AChE activity, using malathion as a model compound, the inhibition of malathion was proportional to its concentration ranging from 0.01 to 0.5 microg/mL and from 1 to 25 microg/mL, with a detection limit of 1.0 ng/mL. The developed biosensor exhibited good reproducibility and acceptable stability, thus providing a new promising tool for analysis of enzyme inhibitors.
ESTHER : Du_2010_Biosens.Bioelectron_25_2503
PubMedSearch : Du_2010_Biosens.Bioelectron_25_2503
PubMedID: 20472422

Title : Purification and preliminary crystallographic analysis of a Penicillium expansum lipase - Bian_2005_Biochim.Biophys.Acta_1752_99
Author(s) : Bian C , Yuan C , Lin L , Lin J , Shi X , Ye X , Huang Z , Huang M
Ref : Biochimica & Biophysica Acta , 1752 :99 , 2005
Abstract : PF898 is a strain of Penicillium expansum optimized for the high level production of Penicillium expansum lipase (PEL). This PEL is unique compared with other lipases in several aspects, For example, the PEL shows low sequence identities (<30%) to all other known lipases, and high percentage of hydrophobic residues in the N-terminal region. The PEL was purified to homogeneity and shown to be 28 kDa by SDS-PAGE. Crystals suitable for X-ray diffraction analysis were obtained by the sitting-drop method of vapor diffusion with ammonia sulfate as the precipitating agent at 298 K. The crystals have tetragonal lattice and unit-cell parameters of a=b=88.09 A, c=126.54 A. Diffraction data were collected to a resolution of 2.08 A on an in-house rotating-anode generator.
ESTHER : Bian_2005_Biochim.Biophys.Acta_1752_99
PubMedSearch : Bian_2005_Biochim.Biophys.Acta_1752_99
PubMedID: 16112629

Title : A color-selectable and site-specific integrative transformation system for gene expression studies in the dematiaceous fungus Wangiella (Exophiala) dermatitidis - Ye_1999_Curr.Genet_36_241
Author(s) : Ye X , Feng B , Szaniszlo PJ
Ref : Curr Genet , 36 :241 , 1999
Abstract : To explore potential virulence factors in the dematiaceous (melanized) fungus Wangiella dermatitidis, we established a gene expression system with properties of homologous transformation and color identification. Using a polyketide synthase gene (WdPKS1) fragment for targeting, we found that 52% of transformants became albinos easily distinguishable from nonspecific transformants. Southern analysis confirmed that the integrations were at the WdPKS1 locus, which however did not affect transformant growth. With a heterologous promoter, P-glaA, enhanced expression of lacZ was found at 37 degrees C. Our results indicated that this system allows the efficient production of isogenic strains for gene function analysis in W. dermatitidis.
ESTHER : Ye_1999_Curr.Genet_36_241
PubMedSearch : Ye_1999_Curr.Genet_36_241
PubMedID: 10541862
Gene_locus related to this paper: exode-PKS1