Teodor R

References (2)

Title : The opium poppy genome and morphinan production - Guo_2018_Science_362_343
Author(s) : Guo L , Winzer T , Yang X , Li Y , Ning Z , He Z , Teodor R , Lu Y , Bowser TA , Graham IA , Ye K
Ref : Science , 362 :343 , 2018
Abstract : Morphinan-based painkillers are derived from opium poppy (Papaver somniferum L.). We report a draft of the opium poppy genome, with 2.72 gigabases assembled into 11 chromosomes with contig N50 and scaffold N50 of 1.77 and 204 megabases, respectively. Synteny analysis suggests a whole-genome duplication at ~7.8 million years ago and ancient segmental or whole-genome duplication(s) that occurred before the Papaveraceae-Ranunculaceae divergence 110 million years ago. Syntenic blocks representative of phthalideisoquinoline and morphinan components of a benzylisoquinoline alkaloid cluster of 15 genes provide insight into how this cluster evolved. Paralog analysis identified P450 and oxidoreductase genes that combined to form the STORR gene fusion essential for morphinan biosynthesis in opium poppy. Thus, gene duplication, rearrangement, and fusion events have led to evolution of specialized metabolic products in opium poppy.
ESTHER : Guo_2018_Science_362_343
PubMedSearch : Guo_2018_Science_362_343
PubMedID: 30166436
Gene_locus related to this paper: papso-a0a4y7jba3 , papso-a0a4y7jac2 , papso-a0a4y7kic2

Title : A Papaver somniferum 10-gene cluster for synthesis of the anticancer alkaloid noscapine - Winzer_2012_Science_336_1704
Author(s) : Winzer T , Gazda V , He Z , Kaminski F , Kern M , Larson TR , Li Y , Meade F , Teodor R , Vaistij FE , Walker C , Bowser TA , Graham IA
Ref : Science , 336 :1704 , 2012
Abstract : Noscapine is an antitumor alkaloid from opium poppy that binds tubulin, arrests metaphase, and induces apoptosis in dividing human cells. Elucidation of the biosynthetic pathway will enable improvement in the commercial production of noscapine and related bioactive molecules. Transcriptomic analysis revealed the exclusive expression of 10 genes encoding five distinct enzyme classes in a high noscapine-producing poppy variety, HN1. Analysis of an F(2) mapping population indicated that these genes are tightly linked in HN1, and bacterial artificial chromosome sequencing confirmed that they exist as a complex gene cluster for plant alkaloids. Virus-induced gene silencing resulted in accumulation of pathway intermediates, allowing gene function to be linked to noscapine synthesis and a novel biosynthetic pathway to be proposed.
ESTHER : Winzer_2012_Science_336_1704
PubMedSearch : Winzer_2012_Science_336_1704
PubMedID: 22653730
Gene_locus related to this paper: papso-cxe1