Yoshino N

References (2)

Title : Demonstration of In Vitro Resurrection of Aged Acetylcholinesterase after Exposure to Organophosphorus Chemical Nerve Agents - Zhuang_2018_J.Med.Chem_61_7034
Author(s) : Zhuang Q , Franjesevic AJ , Corrigan TS , Coldren WH , Dicken R , Sillart S , DeYong A , Yoshino N , Smith J , Fabry S , Fitzpatrick K , Blanton TG , Joseph J , Yoder RJ , McElroy CA , Ekici OD , Callam CS , Hadad CM
Ref : Journal of Medicinal Chemistry , 61 :7034 , 2018
Abstract : After the inhibition of acetylcholinesterase (AChE) by organophosphorus (OP) nerve agents, a dealkylation reaction of the phosphylated serine, referred to as aging, can occur. When aged, known reactivators of OP-inhibited AChE are no longer effective. Realkylation of aged AChE may provide a route to reversing aging. We designed and synthesized a library of quinone methide precursors (QMPs) as proposed realkylators of aged AChE. Our lead compound (C8) from an in vitro screen successfully resurrected 32.7 and 20.4% of the activity of methylphosphonate-aged and isopropyl phosphate-aged electric-eel AChE, respectively, after 4 days. C8 displays properties of both resurrection (recovery from the aged to the native state) and reactivation (recovery from the inhibited to the native state). Resurrection of methylphosphonate-aged AChE by C8 was significantly pH-dependent, recovering 21% of activity at 4 mM and pH 9 after only 1 day. C8 is also effective against isopropyl phosphate-aged human AChE.
ESTHER : Zhuang_2018_J.Med.Chem_61_7034
PubMedSearch : Zhuang_2018_J.Med.Chem_61_7034
PubMedID: 29870665

Title : Regulation of self-incompatibility by acetylcholine and cAMP in Lilium longiflorum - Tezuka_2007_J.Plant.Physiol_164_878
Author(s) : Tezuka T , Akita I , Yoshino N , Suzuki Y
Ref : J Plant Physiol , 164 :878 , 2007
Abstract : Elongation of pollen tubes in pistils of Lilium longiflorum cv. Hinomoto after self-incompatible pollination was here found to be promoted by acetylcholine (ACh) and other choline derivatives, such as acetylthiocholine, l-alpha-phosphatidylcholine and chlorocholinechloride [CCC; (2-chloroethyl) trimethyl ammonium chloride]. Moreover, the elongation was promoted by neostigmine, a potent inhibitor of acetylcholinesterase (AChE; acetylcholine-decomposing enzyme) (EC and activities of this and choline acetyltransferase (ChAT; acetylcholine-forming enzyme) (EC in pistils were associated with self-incompatibility. The activity of ChAT was lower after self-incompatible as compared with cross-compatible pollination. Application of cAMP promoted ChAT activities in both cases, whereas activity of AChE in pistils after self-pollination was higher than that after cross-compatible pollination and was suppressed by cAMP in both cases. Furthermore, AChE activity was inhibited by treatment with neostigmine or heating. Our results indicate that the self-incompatibility with self-pollination is due to decrease of ACh and cAMP, causing reduction of ChAT and AC (adenylate cyclase) and concise elevation of AChE and PDE (cAMP phosphodiesterase), and therefore suppressed growth of pollen tubes.
ESTHER : Tezuka_2007_J.Plant.Physiol_164_878
PubMedSearch : Tezuka_2007_J.Plant.Physiol_164_878
PubMedID: 16882455