psepu-clcd1

Pseudomonas putida Pseudomonas sp. B13 dienelactone hydrolase clcD gene

Comment

Relationship

Family : Dienelactone_hydrolase

Block : X

Position in NCBI Life Tree : Pseudomonas putida

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Bacteria N E > Proteobacteria N E > Gammaproteobacteria N E > Pseudomonadales N E > Pseudomonadaceae N E > Pseudomonas N E > Pseudomonas putida group N E > Pseudomonas putida N E

Molecular evidence

No mutation

1DIN - Pseudomonas sp. B13 dienelactone hydrolase 1GGV - Pseudomonas sp. B13 C123S mutant of Dienelactone hydrolase with PMS 4U2B - Crystal structure of dienelactone hydrolase (C123S) at 1.70 A resolution 4U2C - Crystal structure of dienelactone hydrolase A-6 variant (S7T, A24V, Q35H, F38L, Q110L, C123S, Y145C, E199G and S208G) at 1.95 A resolution 4U2D - Crystal structure of dienelactone hydrolase S-2 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G and G211D) at 1.67 A resolution 4U2E - Crystal structure of dienelactone hydrolase S-3 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G, G211D and K234N) at 1.70 A resolution 4U2F - Crystal structure of dienelactone hydrolase B-1 variant (Q35H, F38L, Y64H, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G and G211D) at 1.80 A resolution 4U2G - Crystal structure of dienelactone hydrolase B-4 variant (Q35H, F38L, Y64H, Q76L, Q110L, C123S, Y137C, A141V, Y145C, N154D, E199G, S208G, G211D, S233G and 237Q) at 1.80 A resolution 4P93 - Structure of Dienelactone Hydrolase at 1.85 A resolution crystallised in the C2 space group 4P92 - Crystal structure of dienelactone hydrolase C123S mutant at 1.65 A resolution 1ZI6 - Crystal Structure Analysis of the dienelactone hydrolase (C123S) mutant - 1.7 A. 1ZI9 - Crystal Structure Analysis of the dienelactone hydrolase (E36D, C123S) mutant - 1.5 A. 1ZIC - Crystal Structure Analysis of the dienelactone hydrolase (C123S, R206A) mutant - 1.7 A. 1ZIX - Crystal Structure Analysis of the dienelactone hydrolase mutant (E36D, R105H, C123S, G211D, K234N)- 1.8 A. 1ZI8 - Crystal Structure Analysis of the dienelactone hydrolase mutant(E36D, C123S, A134T, S208G, A229V, K234R)- 1.4 A. 1ZIY - Crystal Structure Analysis of the dienelactone hydrolase mutant (C123S) bound with the PMS moiety of the protease inhibitor, Phenylmethylsulfonyl fluoride (PMSF) - 1.9 A. 1ZJ4 - Crystal Structure Analysis of the dienelactone hydrolase mutant (E36D, C123S) bound with the PMS moiety of the protease inhibitor, Phenylmethylsulfonyl fluoride (PMSF) - 1.7 A. 1ZJ5 - Crystal Structure Analysis of the dienelactone hydrolase mutant (E36D, C123S, A134T, S208G, A229V, K234R) bound with the PMS moiety of the protease inhibitor, Phenylmethylsulfonyl fluoride (PMSF) - 1.7 A.

No kinetic

No disease

Database

Sequence

Peptide

MLTEGISIQS YDGHTFGALV GSPAKAPAPV IVIAQEIFGV NAFMRETVSW LVDQGYAAVC PDLYARQAPG TALDPQDERQ REQAYKLWQA FDMEAGVGDL EAAIRYARHQ PYSNGKVGLV GYCLGGALAF LVAAKGYVDR AVGYYGVGLE KQLKKVPEVK HPALFHMGGQ DHFVPAPSRQ LITEGFGANP LLQVHWYEEA GHSFARTSSS GYVASAAALA NERRLDFLAP LQSKKP

References (16)

Title : Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multidirectional search - Porter_2015_ACS.Chem.Biol_10_611
Author(s) : Porter JL , Boon PL , Murray TP , Huber T , Collyer CA , Ollis DL
Ref : ACS Chemical Biology , 10 :611 , 2015
PubMedID: 25419863
Gene_locus related to this paper: psepu-clcd1

Title : Crystallization of dienelactone hydrolase in two space groups: structural changes caused by crystal packing - Porter_2014_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_70_884
Author(s) : Porter JL , Carr PD , Collyer CA , Ollis DL
Ref : Acta Crystallographica F Struct Biol Commun , 70 :884 , 2014
PubMedID: 25005082
Gene_locus related to this paper: psepu-clcd1

Title : Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase - Kim_2005_Acta.Crystallogr.D.Biol.Crystallogr_61_920
Author(s) : Kim HK , Liu JW , Carr PD , Ollis DL
Ref : Acta Crystallographica D Biol Crystallogr , 61 :920 , 2005
PubMedID: 15983415
Gene_locus related to this paper: psepu-clcd1

Title : Characterization of two alternative promoters for integrase expression in the clc genomic island of Pseudomonas sp. strain B13 - Sentchilo_2003_Mol.Microbiol_49_93
Author(s) : Sentchilo V , Zehnder AJ , van der Meer JR
Ref : Molecular Microbiology , 49 :93 , 2003
PubMedID: 12823813
Gene_locus related to this paper: psepu-clcd1

Title : Structure of the C123S mutant of dienelactone hydrolase (DLH) bound with the PMS moiety of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF) - Robinson_2000_Acta.Crystallogr.D.Biol.Crystallogr_56_1376
Author(s) : Robinson A , Edwards KJ , Carr PD , Barton JD , Ewart GD , Ollis DL
Ref : Acta Crystallographica D Biol Crystallogr , 56 :1376 , 2000
PubMedID: 11053834
Gene_locus related to this paper: psepu-clcd1

Title : Int-B13, an unusual site-specific recombinase of the bacteriophage P4 integrase family, is responsible for chromosomal insertion of the 105-kilobase clc element of Pseudomonas sp. Strain B13 - Ravatn_1998_J.Bacteriol_180_5505
Author(s) : Ravatn R , Studer S , Zehnder AJ , van der Meer JR
Ref : Journal of Bacteriology , 180 :5505 , 1998
PubMedID: 9791097
Gene_locus related to this paper: psepu-clcd1

Title : Cloning, characterization, and sequence analysis of the clcE gene encoding the maleylacetate reductase of Pseudomonas sp. strain B13 - Kasberg_1997_J.Bacteriol_179_3801
Author(s) : Kasberg T , Seibert V , Schlomann M , Reineke W
Ref : Journal of Bacteriology , 179 :3801 , 1997
PubMedID: 9171435
Gene_locus related to this paper: psepu-clcd1

Title : Substrate-induced activation of dienelactone hydrolase: an enzyme with a naturally occurring Cys-His-Asp triad - Cheah_1993_Protein.Eng_6_575
Author(s) : Cheah E , Austin C , Ashley GW , Ollis D
Ref : Protein Engineering , 6 :575 , 1993
PubMedID: 8234228
Gene_locus related to this paper: psepu-clcd1

Title : Catalysis by dienelactone hydrolase: a variation on the protease mechanism - Cheah_1993_Proteins_16_64
Author(s) : Cheah E , Ashley GW , Gary J , Ollis D
Ref : Proteins , 16 :64 , 1993
PubMedID: 8497485
Gene_locus related to this paper: psepu-clcd1

Title : Thiol protease-like active site found in the enzyme dienelactone hydrolase: localization using biochemical, genetic, and structural tools - Pathak_1991_Proteins_9_267
Author(s) : Pathak D , Ashley G , Ollis D
Ref : Proteins , 9 :267 , 1991
PubMedID: 1866431
Gene_locus related to this paper: psepu-clcd1

Title : Refined structure of dienelactone hydrolase at 1.8 A - Pathak_1990_J.Mol.Biol_214_497
Author(s) : Pathak D , Ollis D
Ref : Journal of Molecular Biology , 214 :497 , 1990
PubMedID: 2380986
Gene_locus related to this paper: psepu-clcd1

Title : X-ray crystallographic structure of dienelactone hydrolase at 2.8 A - Pathak_1988_J.Mol.Biol_204_435
Author(s) : Pathak D , Ngai KL , Ollis D
Ref : Journal of Molecular Biology , 204 :435 , 1988
PubMedID: 3221394
Gene_locus related to this paper: psepu-clcd1

Title : Nucleotide sequence and expression of clcD, a plasmid-borne dienelactone hydrolase gene from Pseudomonas sp. strain B13 - Frantz_1987_J.Bacteriol_169_704
Author(s) : Frantz B , Ngai KL , Chatterjee DK , Ornston LN , Chakrabarty AM
Ref : Journal of Bacteriology , 169 :704 , 1987
PubMedID: 3804974
Gene_locus related to this paper: psepu-clcd1

Title : Dienelactone hydrolase from Pseudomonas sp. strain B13 - Ngai_1987_J.Bacteriol_169_699
Author(s) : Ngai KL , Schlomann M , Knackmuss HJ , Ornston LN
Ref : Journal of Bacteriology , 169 :699 , 1987
PubMedID: 3804973
Gene_locus related to this paper: psepu-clcd1

Title : Organization and nucleotide sequence determination of a gene cluster involved in 3-chlorocatechol degradation - Frantz_1987_Proc.Natl.Acad.Sci.U.S.A_84_4460
Author(s) : Frantz B , Chakrabarty AM
Ref : Proceedings of the National Academy of Sciences of the United States of America , 84 :4460 , 1987
PubMedID: 3299368
Gene_locus related to this paper: psepu-clcd1

Title : Crystallization and preliminary x-ray crystallographic data of dienelactone hydrolase from Pseudomonas sp. B13 - Ollis_1985_J.Biol.Chem_260_9818
Author(s) : Ollis DL , Ngai KL
Ref : Journal of Biological Chemistry , 260 :9818 , 1985
PubMedID: 4019496
Gene_locus related to this paper: psepu-clcd1