psepu-clcd1
Pseudomonas putida Pseudomonas sp. B13 dienelactone hydrolase clcD gene
Comment
Relationship
Family : Dienelactone_hydrolase
Block : X
Position in NCBI Life Tree : Pseudomonas putida
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Proteobacteria N E > Gammaproteobacteria N E > Pseudomonadales N E > Pseudomonadaceae N E > Pseudomonas N E > Pseudomonas putida group N E > Pseudomonas putida N EMolecular evidence
No mutation
1DIN - Pseudomonas sp. B13 dienelactone hydrolase
1GGV - Pseudomonas sp. B13 C123S mutant of Dienelactone hydrolase with PMS
1ZI6 - Crystal Structure Analysis of the dienelactone hydrolase (C123S) mutant - 1.7 A.
1ZI8 - Crystal Structure Analysis of the dienelactone hydrolase mutant(E36D, C123S, A134T, S208G, A229V, K234R)- 1.4 A.
1ZI9 - Crystal Structure Analysis of the dienelactone hydrolase (E36D, C123S) mutant - 1.5 A.
1ZIC - Crystal Structure Analysis of the dienelactone hydrolase (C123S, R206A) mutant - 1.7 A.
1ZIX - Crystal Structure Analysis of the dienelactone hydrolase mutant (E36D, R105H, C123S, G211D, K234N)- 1.8 A.
1ZIY - Crystal Structure Analysis of the dienelactone hydrolase mutant (C123S) bound with the PMS moiety of the protease inhibitor, Phenylmethylsulfonyl fluoride (PMSF) - 1.9 A.
1ZJ4 - Crystal Structure Analysis of the dienelactone hydrolase mutant (E36D, C123S) bound with the PMS moiety of the protease inhibitor, Phenylmethylsulfonyl fluoride (PMSF) - 1.7 A.
1ZJ5 - Crystal Structure Analysis of the dienelactone hydrolase mutant (E36D, C123S, A134T, S208G, A229V, K234R) bound with the PMS moiety of the protease inhibitor, Phenylmethylsulfonyl fluoride (PMSF) - 1.7 A.
4P92 - Crystal structure of dienelactone hydrolase C123S mutant at 1.65 A resolution
4P93 - Structure of Dienelactone Hydrolase at 1.85 A resolution crystallised in the C2 space group
4U2B - Crystal structure of dienelactone hydrolase (C123S) at 1.70 A resolution
4U2C - Crystal structure of dienelactone hydrolase A-6 variant (S7T, A24V, Q35H, F38L, Q110L, C123S, Y145C, E199G and S208G) at 1.95 A resolution
4U2D - Crystal structure of dienelactone hydrolase S-2 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G and G211D) at 1.67 A resolution
4U2E - Crystal structure of dienelactone hydrolase S-3 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G, G211D and K234N) at 1.70 A resolution
4U2F - Crystal structure of dienelactone hydrolase B-1 variant (Q35H, F38L, Y64H, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G and G211D) at 1.80 A resolution
4U2G - Crystal structure of dienelactone hydrolase B-4 variant (Q35H, F38L, Y64H, Q76L, Q110L, C123S, Y137C, A141V, Y145C, N154D, E199G, S208G, G211D, S233G and 237Q) at 1.80 A resolution
No kinetic
No disease
Sequence
Peptide
MLTEGISIQS YDGHTFGALV GSPAKAPAPV IVIAQEIFGV NAFMRETVSW LVDQGYAAVC PDLYARQAPG TALDPQDERQ REQAYKLWQA FDMEAGVGDL EAAIRYARHQ PYSNGKVGLV GYCLGGALAF LVAAKGYVDR AVGYYGVGLE KQLKKVPEVK HPALFHMGGQ DHFVPAPSRQ LITEGFGANP LLQVHWYEEA GHSFARTSSS GYVASAAALA NERRLDFLAP LQSKKP
References (16)
| Title : Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multidirectional search - Porter_2015_ACS.Chem.Biol_10_611 |
| Author(s) : Porter JL , Boon PL , Murray TP , Huber T , Collyer CA , Ollis DL |
| Ref : ACS Chemical Biology , 10 :611 , 2015 |
| Abstract : |
| PubMedSearch : Porter_2015_ACS.Chem.Biol_10_611 |
| PubMedID: 25419863 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Crystallization of dienelactone hydrolase in two space groups: structural changes caused by crystal packing - Porter_2014_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_70_884 |
| Author(s) : Porter JL , Carr PD , Collyer CA , Ollis DL |
| Ref : Acta Crystallographica F Struct Biol Commun , 70 :884 , 2014 |
| Abstract : |
| PubMedSearch : Porter_2014_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_70_884 |
| PubMedID: 25005082 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase - Kim_2005_Acta.Crystallogr.D.Biol.Crystallogr_61_920 |
| Author(s) : Kim HK , Liu JW , Carr PD , Ollis DL |
| Ref : Acta Crystallographica D Biol Crystallogr , 61 :920 , 2005 |
| Abstract : |
| PubMedSearch : Kim_2005_Acta.Crystallogr.D.Biol.Crystallogr_61_920 |
| PubMedID: 15983415 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Characterization of two alternative promoters for integrase expression in the clc genomic island of Pseudomonas sp. strain B13 - Sentchilo_2003_Mol.Microbiol_49_93 |
| Author(s) : Sentchilo V , Zehnder AJ , van der Meer JR |
| Ref : Molecular Microbiology , 49 :93 , 2003 |
| Abstract : |
| PubMedSearch : Sentchilo_2003_Mol.Microbiol_49_93 |
| PubMedID: 12823813 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Structure of the C123S mutant of dienelactone hydrolase (DLH) bound with the PMS moiety of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF) - Robinson_2000_Acta.Crystallogr.D.Biol.Crystallogr_56_1376 |
| Author(s) : Robinson A , Edwards KJ , Carr PD , Barton JD , Ewart GD , Ollis DL |
| Ref : Acta Crystallographica D Biol Crystallogr , 56 :1376 , 2000 |
| Abstract : |
| PubMedSearch : Robinson_2000_Acta.Crystallogr.D.Biol.Crystallogr_56_1376 |
| PubMedID: 11053834 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Int-B13, an unusual site-specific recombinase of the bacteriophage P4 integrase family, is responsible for chromosomal insertion of the 105-kilobase clc element of Pseudomonas sp. Strain B13 - Ravatn_1998_J.Bacteriol_180_5505 |
| Author(s) : Ravatn R , Studer S , Zehnder AJ , van der Meer JR |
| Ref : Journal of Bacteriology , 180 :5505 , 1998 |
| Abstract : |
| PubMedSearch : Ravatn_1998_J.Bacteriol_180_5505 |
| PubMedID: 9791097 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Cloning, characterization, and sequence analysis of the clcE gene encoding the maleylacetate reductase of Pseudomonas sp. strain B13 - Kasberg_1997_J.Bacteriol_179_3801 |
| Author(s) : Kasberg T , Seibert V , Schlomann M , Reineke W |
| Ref : Journal of Bacteriology , 179 :3801 , 1997 |
| Abstract : |
| PubMedSearch : Kasberg_1997_J.Bacteriol_179_3801 |
| PubMedID: 9171435 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Substrate-induced activation of dienelactone hydrolase: an enzyme with a naturally occurring Cys-His-Asp triad - Cheah_1993_Protein.Eng_6_575 |
| Author(s) : Cheah E , Austin C , Ashley GW , Ollis D |
| Ref : Protein Engineering , 6 :575 , 1993 |
| Abstract : |
| PubMedSearch : Cheah_1993_Protein.Eng_6_575 |
| PubMedID: 8234228 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Catalysis by dienelactone hydrolase: a variation on the protease mechanism - Cheah_1993_Proteins_16_64 |
| Author(s) : Cheah E , Ashley GW , Gary J , Ollis D |
| Ref : Proteins , 16 :64 , 1993 |
| Abstract : |
| PubMedSearch : Cheah_1993_Proteins_16_64 |
| PubMedID: 8497485 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Thiol protease-like active site found in the enzyme dienelactone hydrolase: localization using biochemical, genetic, and structural tools - Pathak_1991_Proteins_9_267 |
| Author(s) : Pathak D , Ashley G , Ollis D |
| Ref : Proteins , 9 :267 , 1991 |
| Abstract : |
| PubMedSearch : Pathak_1991_Proteins_9_267 |
| PubMedID: 1866431 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Refined structure of dienelactone hydrolase at 1.8 A - Pathak_1990_J.Mol.Biol_214_497 |
| Author(s) : Pathak D , Ollis D |
| Ref : Journal of Molecular Biology , 214 :497 , 1990 |
| Abstract : |
| PubMedSearch : Pathak_1990_J.Mol.Biol_214_497 |
| PubMedID: 2380986 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : X-ray crystallographic structure of dienelactone hydrolase at 2.8 A - Pathak_1988_J.Mol.Biol_204_435 |
| Author(s) : Pathak D , Ngai KL , Ollis D |
| Ref : Journal of Molecular Biology , 204 :435 , 1988 |
| Abstract : |
| PubMedSearch : Pathak_1988_J.Mol.Biol_204_435 |
| PubMedID: 3221394 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Nucleotide sequence and expression of clcD, a plasmid-borne dienelactone hydrolase gene from Pseudomonas sp. strain B13 - Frantz_1987_J.Bacteriol_169_704 |
| Author(s) : Frantz B , Ngai KL , Chatterjee DK , Ornston LN , Chakrabarty AM |
| Ref : Journal of Bacteriology , 169 :704 , 1987 |
| Abstract : |
| PubMedSearch : Frantz_1987_J.Bacteriol_169_704 |
| PubMedID: 3804974 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Dienelactone hydrolase from Pseudomonas sp. strain B13 - Ngai_1987_J.Bacteriol_169_699 |
| Author(s) : Ngai KL , Schlomann M , Knackmuss HJ , Ornston LN |
| Ref : Journal of Bacteriology , 169 :699 , 1987 |
| Abstract : |
| PubMedSearch : Ngai_1987_J.Bacteriol_169_699 |
| PubMedID: 3804973 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Organization and nucleotide sequence determination of a gene cluster involved in 3-chlorocatechol degradation - Frantz_1987_Proc.Natl.Acad.Sci.U.S.A_84_4460 |
| Author(s) : Frantz B , Chakrabarty AM |
| Ref : Proceedings of the National Academy of Sciences of the United States of America , 84 :4460 , 1987 |
| Abstract : |
| PubMedSearch : Frantz_1987_Proc.Natl.Acad.Sci.U.S.A_84_4460 |
| PubMedID: 3299368 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : Crystallization and preliminary x-ray crystallographic data of dienelactone hydrolase from Pseudomonas sp. B13 - Ollis_1985_J.Biol.Chem_260_9818 |
| Author(s) : Ollis DL , Ngai KL |
| Ref : Journal of Biological Chemistry , 260 :9818 , 1985 |
| Abstract : |
| PubMedSearch : Ollis_1985_J.Biol.Chem_260_9818 |
| PubMedID: 4019496 |
| Gene_locus related to this paper: psepu-clcd1 |