Dienelactone_hydrolase

Relationship

Family: Dienelactone_hydrolase

Block: X

Parent Family: No family

Comment

Dienelactone hydrolases play a crucial role in chlorocatechol degradation via the modified ortho cleavage pathway. Enzymes induced in 4-fluorobenzoate-utilizing bacteria have been classified into three groups on the basis of their specificity towards cis- and trans-dienelactone. The catalytic triad of the prototype psepu-clcd1 consists of Cys123, His202 and Asp171

Database

Interpro : IPR002925 Dienelactone hydrolase

PIRSF : No PIRSF

Pdoc : No Pdoc

Pfam : PF01738 DLH

Prints : No Print

EC Number : 3.1.1.45

Sequences

Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour
Alignment with MAFFT No colour
Dendrogram The dnd file

Structures (23)

Genes Proteins in Dienelactone_hydrolase family (431)

Fragments of genes in Dienelactone_hydrolase family (15)

Structures in Dienelactone_hydrolase family (23)

Substrates in Dienelactone_hydrolase family (3)

Inhibitors in Dienelactone_hydrolase family (2)

References (11)

Title : Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multidirectional search - Porter_2015_ACS.Chem.Biol_10_611
Author(s) : Porter JL , Boon PL , Murray TP , Huber T , Collyer CA , Ollis DL
Ref : ACS Chemical Biology , 10 :611 , 2015
PubMedID: 25419863
Gene_locus related to this paper: psepu-clcd1

Title : Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics - Colin_2015_Nat.Commun_6_10008
Author(s) : Colin PY , Kintses B , Gielen F , Miton CM , Fischer G , Mohamed MF , Hyvonen M , Morgavi DP , Janssen DB , Hollfelder F
Ref : Nat Commun , 6 :10008 , 2015
PubMedID: 26639611
Gene_locus related to this paper: 9bact-KP212148

Title : Crystallization of dienelactone hydrolase in two space groups: structural changes caused by crystal packing - Porter_2014_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_70_884
Author(s) : Porter JL , Carr PD , Collyer CA , Ollis DL
Ref : Acta Crystallographica F Struct Biol Commun , 70 :884 , 2014
PubMedID: 25005082
Gene_locus related to this paper: psepu-clcd1

Title : Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase - Kim_2005_Acta.Crystallogr.D.Biol.Crystallogr_61_920
Author(s) : Kim HK , Liu JW , Carr PD , Ollis DL
Ref : Acta Crystallographica D Biol Crystallogr , 61 :920 , 2005
PubMedID: 15983415
Gene_locus related to this paper: psepu-clcd1

Title : Monitoring key reactions in degradation of chloroaromatics by in situ (1)H nuclear magnetic resonance: solution structures of metabolites formed from cis-dienelactone - Pieper_2002_J.Bacteriol_184_1466
Author(s) : Pieper DH , Pollmann K , Nikodem P , Gonzalez B , Wray V
Ref : Journal of Bacteriology , 184 :1466 , 2002
PubMedID: 11844781

Title : Structure of the C123S mutant of dienelactone hydrolase (DLH) bound with the PMS moiety of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF) - Robinson_2000_Acta.Crystallogr.D.Biol.Crystallogr_56_1376
Author(s) : Robinson A , Edwards KJ , Carr PD , Barton JD , Ewart GD , Ollis DL
Ref : Acta Crystallographica D Biol Crystallogr , 56 :1376 , 2000
PubMedID: 11053834
Gene_locus related to this paper: psepu-clcd1

Title : Evolution of chlorocatechol catabolic pathways. Conclusions to be drawn from comparisons of lactone hydrolases - Schlomann_1994_Biodegradation_5_301
Author(s) : Schlomann M
Ref : Biodegradation , 5 :301 , 1994
PubMedID: 7765840

Title : Substrate-induced activation of dienelactone hydrolase: an enzyme with a naturally occurring Cys-His-Asp triad - Cheah_1993_Protein.Eng_6_575
Author(s) : Cheah E , Austin C , Ashley GW , Ollis D
Ref : Protein Engineering , 6 :575 , 1993
PubMedID: 8234228
Gene_locus related to this paper: psepu-clcd1

Title : Catalysis by dienelactone hydrolase: a variation on the protease mechanism - Cheah_1993_Proteins_16_64
Author(s) : Cheah E , Ashley GW , Gary J , Ollis D
Ref : Proteins , 16 :64 , 1993
PubMedID: 8497485
Gene_locus related to this paper: psepu-clcd1

Title : Refined structure of dienelactone hydrolase at 1.8 A - Pathak_1990_J.Mol.Biol_214_497
Author(s) : Pathak D , Ollis D
Ref : Journal of Molecular Biology , 214 :497 , 1990
PubMedID: 2380986
Gene_locus related to this paper: psepu-clcd1

Title : X-ray crystallographic structure of dienelactone hydrolase at 2.8 A - Pathak_1988_J.Mol.Biol_204_435
Author(s) : Pathak D , Ngai KL , Ollis D
Ref : Journal of Molecular Biology , 204 :435 , 1988
PubMedID: 3221394
Gene_locus related to this paper: psepu-clcd1