Gene_locus Report for: 9baci-a0a060lzd7

Other strains: Bacillus lehensis G1; Bacillus sp. (JCM 19047; JCM 19046; JCM 19045)

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Bacteria: N E > Terrabacteria group: N E > Firmicutes: N E > Bacilli: N E > Bacillales: N E > Bacillaceae: N E > Bacillus: N E > Bacillus lehensis: N E > Bacillus lehensis G1: N E

Title: Draft Genome Sequences of Cyclodextrin-Producing Alkaliphilic Bacillus Strains JCM 19045, JCM 19046, and JCM 19047
Kudo T, Sakamoto K, Akinaga M, Kawauchi A, Nakahara T, Zhang X, Yamada A, Oshima K, Suda W and Ohkuma M <10 more author(s)> Kudo T, Sakamoto K, Akinaga M, Kawauchi A, Nakahara T, Zhang X, Yamada A, Oshima K, Suda W, Kuwahara H, Nakamura N, Nogi Y, Kitamura K, Yuki M, Iida T, Moriya S, Inoue T, Hongoh Y, Hattori M, Ohkuma M (- 10)
Ref: Genome Announc, 2:, 2014 : PubMed
Abstract


ESTHER: Kudo_2014_Genome.Announc_2_e00211
PubMedSearch: Kudo 2014 Genome.Announc 2 e00211
PubMedID: 24652985
Gene_locus related to this paper: 9baci-a0a060lzd7, 9baci-w7yjr4, 9baci-w7zpe5, 9baci-w8a608, 9baci-w7zad0

Abstract

Bacillus strains JCM 19045, JCM 19046, and JCM 19047 are alkaliphiles that produce beta-cyclodextrin from starch. They are related to Bacillus xiaoxiensis and Bacillus lehensis. The genome information for these three strains will be useful for studies of the physiological role of cyclodextrin and cyclodextrin production.



        

Title: A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1
Noor YM, Samsulrizal NH, Jema'on NA, Low KO, Ramli AN, Alias NI, Damis SI, Fuzi SF, Isa MN and Illias RM <5 more author(s)> Noor YM, Samsulrizal NH, Jema'on NA, Low KO, Ramli AN, Alias NI, Damis SI, Fuzi SF, Isa MN, Murad AM, Raih MF, Bakar FD, Najimudin N, Mahadi NM, Illias RM (- 5)
Ref: Gene, 545:253, 2014 : PubMed
Abstract


ESTHER: Noor_2014_Gene_545_253
PubMedSearch: Noor 2014 Gene 545 253
PubMedID: 24811681
Gene_locus related to this paper: 9baci-a0a060lzd7, 9baci-a0a060m4t3, 9baci-a0a060lz75

Abstract

Bacillus lehensis G1 is a Gram-positive, moderately alkalitolerant bacterium isolated from soil samples. B. lehensis produces cyclodextrin glucanotransferase (CGTase), an enzyme that has enabled the extensive use of cyclodextrin in foodstuffs, chemicals, and pharmaceuticals. The genome sequence of B. lehensis G1 consists of a single circular 3.99 Mb chromosome containing 4017 protein-coding sequences (CDSs), of which 2818 (70.15%) have assigned biological roles, 936 (23.30%) have conserved domains with unknown functions, and 263 (6.55%) have no match with any protein database. Bacillus clausii KSM-K16 was established as the closest relative to B. lehensis G1 based on gene content similarity and 16S rRNA phylogenetic analysis. A total of 2820 proteins from B. lehensis G1 were found to have orthologues in B. clausii, including sodium-proton antiporters, transport proteins, and proteins involved in ATP synthesis. A comparative analysis of these proteins and those in B. clausii and other alkaliphilic Bacillus species was carried out to investigate their contributions towards the alkalitolerance of the microorganism. The similarities and differences in alkalitolerance-related genes among alkalitolerant/alkaliphilic Bacillus species highlight the complex mechanism of pH homeostasis. The B. lehensis G1 genome was also mined for proteins and enzymes with potential viability for industrial and commercial purposes.