Oshima K

References (48)

Title : Cholesteryl Ester Storage Disease: An underdiagnosed cause of cirrhosis in adults - Pant_2017_Ann.Diagn.Pathol_31_66
Author(s) : Pant M , Oshima K
Ref : Ann Diagn Pathol , 31 :66 , 2017
Abstract : Cholesteryl Ester Storage Disease (CESD), is a rare multisystem autosomal recessive disorder and belongs to the broad family of lysosomal storage disorders. It can present anytime from infancy and childhood to even adulthood. The clinical manifestations are generally severe in infants and with milder forms in adults. One of the prominent sites of involvement is liver. Due to low awareness of this condition among physicians including surgical pathologists, majority of the liver biopsies, especially from the adults are often misdiagnosed as non-alcoholic fatty liver disease/non-alcoholic steatohepatitis or cryptogenic cirrhosis. Given the recent availability of safe and effective enzyme replacement therapy that can alter the natural course of CESD, the pathologists signing out adult and pediatric liver biopsies should be aware of this entity, thus contributing to timely patient management. This review discusses the clinical features, pathogenesis, diagnostic approach, differential diagnosis and management of CESD in adults.
ESTHER : Pant_2017_Ann.Diagn.Pathol_31_66
PubMedSearch : Pant_2017_Ann.Diagn.Pathol_31_66
PubMedID: 28318950
Gene_locus related to this paper: human-LIPA

Title : Genome analysis of a novel Bradyrhizobium sp. DOA9 carrying a symbiotic plasmid - Okazaki_2015_PLoS.One_10_e0117392
Author(s) : Okazaki S , Noisangiam R , Okubo T , Kaneko T , Oshima K , Hattori M , Teamtisong K , Songwattana P , Tittabutr P , Boonkerd N , Saeki K , Sato S , Uchiumi T , Minamisawa K , Teaumroong N
Ref : PLoS ONE , 10 :e0117392 , 2015
Abstract : Bradyrhizobium sp. DOA9 isolated from the legume Aeschynomene americana exhibited a broad host range and divergent nodulation (nod) genes compared with other members of the Bradyrhizobiaceae. Genome analysis of DOA9 revealed that its genome comprised a single chromosome of 7.1 Mbp and a plasmid of 0.7 Mbp. The chromosome showed highest similarity with that of the nod gene-harboring soybean symbiont B. japonicum USDA110, whereas the plasmid showed highest similarity with pBBta01 of the nod gene-lacking photosynthetic strain BTAi1, which nodulates Aeschynomene species. Unlike in other bradyrhizobia, the plasmid of DOA9 encodes genes related to symbiotic functions including nodulation, nitrogen fixation, and type III/IV protein secretion systems. The plasmid has also a lower GC content (60.1%) than the chromosome (64.4%). These features suggest that the plasmid could be the origin of the symbiosis island that is found in the genome of other bradyrhizobia. The nod genes of DOA9 exhibited low similarity with those of other strains. The nif gene cluster of DOA9 showed greatest similarity to those of photosynthetic bradyrhizobia. The type III/IV protein secretion systems of DOA9 are similar to those of nod gene-harboring B. elkanii and photosynthetic BTAi1. The DOA9 genome exhibited intermediate characteristics between nod gene-harboring bradyrhizobia and nod gene-lacking photosynthetic bradyrhizobia, thus providing the evidence for the evolution of the Bradyrhizobiaceae during ecological adaptation. Bradyrhizobium sp. DOA9 isolated from the legume Aeschynomene americana exhibited a broad host range and divergent nodulation (nod) genes compared with other members of the Bradyrhizobiaceae. Genome analysis of DOA9 revealed that its genome comprised a single chromosome of 7.1 Mbp and a plasmid of 0.7 Mbp. The chromosome showed highest similarity with that of the nod gene-harboring soybean symbiont B. japonicum USDA110, whereas the plasmid showed highest similarity with pBBta01 of the nod gene-lacking photosynthetic strain BTAi1, which nodulates Aeschynomene species. Unlike in other bradyrhizobia, the plasmid of DOA9 encodes genes related to symbiotic functions including nodulation, nitrogen fixation, and type III/IV protein secretion systems. The plasmid has also a lower GC content (60.1%) than the chromosome (64.4%). These features suggest that the plasmid could be the origin of the symbiosis island that is found in the genome of other bradyrhizobia. The nod genes of DOA9 exhibited low similarity with those of other strains. The nif gene cluster of DOA9 showed greatest similarity to those of photosynthetic bradyrhizobia. The type III/IV protein secretion systems of DOA9 are similar to those of nod gene-harboring B. elkanii and photosynthetic BTAi1. The DOA9 genome exhibited intermediate characteristics between nod gene-harboring bradyrhizobia and nod gene-lacking photosynthetic bradyrhizobia, thus providing the evidence for the evolution of the Bradyrhizobiaceae during ecological adaptation.
ESTHER : Okazaki_2015_PLoS.One_10_e0117392
PubMedSearch : Okazaki_2015_PLoS.One_10_e0117392
PubMedID: 25710540
Gene_locus related to this paper: 9brad-a0a0r3chf0 , 9brad-a0a0s6v2v2

Title : Distribution and evolution of nitrogen fixation genes in the phylum Bacteroidetes - Inoue_2015_Microbes.Environ_30_44
Author(s) : Inoue J , Oshima K , Suda W , Sakamoto M , Iino T , Noda S , Hongoh Y , Hattori M , Ohkuma M
Ref : Microbes Environ , 30 :44 , 2015
Abstract : Diazotrophs had not previously been identified among bacterial species in the phylum Bacteroidetes until the rapid expansion of bacterial genome sequences, which revealed the presence of nitrogen fixation (nif) genes in this phylum. We herein determined the draft genome sequences of Bacteroides graminisolvens JCM 15093(T) and Geofilum rubicundum JCM 15548(T). In addition to these and previously reported 'Candidatus Azobacteroides pseudotrichonymphae' and Paludibacter propionicigenes, an extensive survey of the genome sequences of diverse Bacteroidetes members revealed the presence of a set of nif genes (nifHDKENB) in strains of Dysgonomonas gadei, Dysgonomonas capnocytophagoides, Saccharicrinis fermentans, and Alkaliflexus imshenetskii. These eight species belonged to and were distributed sporadically within the order Bacteroidales. Acetylene reduction activity was detected in the five species examined, strongly suggesting their diazotrophic nature. Phylogenetic analyses showed monophyletic clustering of the six Nif protein sequences in the eight Bacteroidales species, implying that nitrogen fixation is ancestral to Bacteroidales and has been retained in these species, but lost in many other lineages. The identification of nif genes in Bacteroidales facilitates the prediction of the organismal origins of related sequences directly obtained from various environments.
ESTHER : Inoue_2015_Microbes.Environ_30_44
PubMedSearch : Inoue_2015_Microbes.Environ_30_44
PubMedID: 25736980
Gene_locus related to this paper: 9bact-a0a0e9lvi0 , 9bact-a0a0e9lzx7 , 9bace-a0a069d1v9 , 9bact-a0a0e9lyc6 , 9bace-a0a069d662

Title : Draft Genome Sequence of Streptomyces incarnatus NRRL8089, which Produces the Nucleoside Antibiotic Sinefungin - Oshima_2015_Genome.Announc_3_e00715
Author(s) : Oshima K , Hattori M , Shimizu H , Fukuda K , Nemoto M , Inagaki K , Tamura T
Ref : Genome Announc , 3 : , 2015
Abstract : A draft genome sequence of Streptomyces incarnatus NRRL8089, which produces the nucleoside antibiotic sinefungin, is described here. The genome contains 8,897,465 bp in 76 contigs and 8,266 predicted genes. Interestingly, the genome encodes an open reading frame for selenocysteine-containing formate dehydrogenase-O and the selenoprotein biosynthetic gene cluster selABCD.
ESTHER : Oshima_2015_Genome.Announc_3_e00715
PubMedSearch : Oshima_2015_Genome.Announc_3_e00715
PubMedID: 26159526
Gene_locus related to this paper: 9actn-a0a0g3ac56 , 9actn-a0a0g3anc9 , 9actn-a0a0g3awn7 , 9actn-a0a0g3avt9

Title : Complete Genome Sequence of a Phenanthrene Degrader, Mycobacterium sp. Strain EPa45 (NBRC 110737), Isolated from a Phenanthrene-Degrading Consortium - Kato_2015_Genome.Announc_3_e00782
Author(s) : Kato H , Ogawa N , Ohtsubo Y , Oshima K , Toyoda A , Mori H , Nagata Y , Kurokawa K , Hattori M , Fujiyama A , Tsuda M
Ref : Genome Announc , 3 : , 2015
Abstract : A phenanthrene degrader, Mycobacterium sp. EPa45, was isolated from a phenanthrene-degrading consortium. Here, we report the complete genome sequence of EPa45, which has a 6.2-Mb single circular chromosome. We propose a phenanthrene degradation pathway in EPa45 based on the complete genome sequence.
ESTHER : Kato_2015_Genome.Announc_3_e00782
PubMedSearch : Kato_2015_Genome.Announc_3_e00782
PubMedID: 26184940
Gene_locus related to this paper: 9myco-a0a0g3iim6 , 9myco-a0a0g3ijm3 , 9myco-a0a0g3ipv9 , 9myco-a0a0g3iuf8 , 9myco-a0a0g3ivy5 , 9myco-a0a0g3ihb1

Title : Draft Genome Sequences of Marine Flavobacterium Algibacter lectus Strains SS8 and NR4 - Takatani_2014_Genome.Announc_2_e01168
Author(s) : Takatani N , Nakanishi M , Meirelles P , Mino S , Suda W , Oshima K , Hattori M , Ohkuma M , Hosokawa M , Miyashita K , Thompson FL , Niwa A , Sawabe T
Ref : Genome Announc , 2 : , 2014
Abstract : Here, we present the draft genome sequences of a zeaxanthin-producing flavobacterium, Algibacter lectus strains SS8 and NR4, isolated from coastal sediment and rock surfaces in Hakodate, Japan, respectively. This genomic information represents the first Algibacter genome sequences, which will help us to elucidate the biology and evolution of Flavobacteriaceae bacteria.
ESTHER : Takatani_2014_Genome.Announc_2_e01168
PubMedSearch : Takatani_2014_Genome.Announc_2_e01168
PubMedID: 25395640
Gene_locus related to this paper: 9flao-a0a090w6b8 , 9flao-a0a090wm61

Title : Draft Genome Sequences of Three Alkaliphilic Bacillus Strains, Bacillus wakoensis JCM 9140T, Bacillus akibai JCM 9157T, and Bacillus hemicellulosilyticus JCM 9152T - Yuki_2014_Genome.Announc_2_e01258
Author(s) : Yuki M , Oshima K , Suda W , Oshida Y , Kitamura K , Iida T , Hattori M , Ohkuma M
Ref : Genome Announc , 2 : , 2014
Abstract : Here, we report the draft genome sequences of the type strains of three cellulolytic or hemicellulolytic alkaliphilic Bacillus species: Bacillus wakoensis, Bacillus akibai, and Bacillus hemicellulosilyticus. The genome information for these three strains will be useful for studies of alkaliphilic Bacillus species, their evolution, and biotechnological applications for their enzymes.
ESTHER : Yuki_2014_Genome.Announc_2_e01258
PubMedSearch : Yuki_2014_Genome.Announc_2_e01258
PubMedID: 24482522
Gene_locus related to this paper: 9baci-w4q973 , baca3-w4qlz5 , 9baci-w4qj07 , 9baci-w4q8b8 , baca3-w4qp70 , 9baci-w4qcm9 , baca3-w4qrq6

Title : Draft Genome Sequence of the Boron-Tolerant and Moderately Halotolerant Bacterium Gracilibacillus boraciitolerans JCM 21714T - Ahmed_2014_Genome.Announc_2_e00097
Author(s) : Ahmed I , Oshima K , Suda W , Kitamura K , Iida T , Ohmori Y , Fujiwara T , Hattori M , Ohkuma M
Ref : Genome Announc , 2 : , 2014
Abstract : Gracilibacillus boraciitolerans JCM 21714(T) has been characterized as a highly boron-tolerant and moderately halotolerant bacterium. Here, we report the draft genome sequence of this strain. The genome sequence facilitates an understanding of the biochemical functions of boron and provides a base to identify the gene(s) involved in the boron tolerance mechanism of the strain.
ESTHER : Ahmed_2014_Genome.Announc_2_e00097
PubMedSearch : Ahmed_2014_Genome.Announc_2_e00097
PubMedID: 24558242
Gene_locus related to this paper: 9baci-w4vi05 , 9baci-w4vjh3 , 9baci-w4vgr7

Title : Draft Genome Sequences of Cyclodextrin-Producing Alkaliphilic Bacillus Strains JCM 19045, JCM 19046, and JCM 19047 - Kudo_2014_Genome.Announc_2_e00211
Author(s) : Kudo T , Sakamoto K , Akinaga M , Kawauchi A , Nakahara T , Zhang X , Yamada A , Oshima K , Suda W , Kuwahara H , Nakamura N , Nogi Y , Kitamura K , Yuki M , Iida T , Moriya S , Inoue T , Hongoh Y , Hattori M , Ohkuma M
Ref : Genome Announc , 2 : , 2014
Abstract : Bacillus strains JCM 19045, JCM 19046, and JCM 19047 are alkaliphiles that produce beta-cyclodextrin from starch. They are related to Bacillus xiaoxiensis and Bacillus lehensis. The genome information for these three strains will be useful for studies of the physiological role of cyclodextrin and cyclodextrin production.
ESTHER : Kudo_2014_Genome.Announc_2_e00211
PubMedSearch : Kudo_2014_Genome.Announc_2_e00211
PubMedID: 24652985
Gene_locus related to this paper: 9baci-a0a060lzd7 , 9baci-w7yjr4 , 9baci-w7zpe5 , 9baci-w8a608 , 9baci-w7zad0

Title : Draft Genome Sequences of Geomicrobium sp. Strains JCM 19037, JCM 19038, JCM 19039, and JCM 19055, Isolated from Aquatic Samples - Kudo_2014_Genome.Announc_2_e00622
Author(s) : Kudo T , Nakahara T , Zhang X , Taniyama S , Arakawa O , Murase S , Nakata H , Oshima K , Suda W , Kitamura K , Iida T , Oshida Y , Inoue T , Hongoh Y , Hattori M , Ohkuma M
Ref : Genome Announc , 2 : , 2014
Abstract : Haloalkaliphilic strains JCM 19037, JCM 19038, JCM 19039, and JCM 19055, closely related to Geomicrobium sediminis, were isolated from aquatic samples, and their draft genome sequences were determined. The genome information of these four strains will be useful for studies of their physiology and ecology.
ESTHER : Kudo_2014_Genome.Announc_2_e00622
PubMedSearch : Kudo_2014_Genome.Announc_2_e00622
PubMedID: 24948772
Gene_locus related to this paper: 9bacl-a0a061nv39 , 9bacl-a0a061nsm4 , 9bacl-a0a061ndf6 , 9bacl-a0a061nlu5 , 9bacl-a0a061ntb4 , 9bacl-a0a061nnc9 , 9bacl-a0a061pf66

Title : Draft Genome Sequences of Two Lactobacillus Strains, L. farraginis JCM 14108T and L. composti JCM 14202T, Isolated from Compost of Distilled Shochu Residue - Yuki_2014_Genome.Announc_2_e00257
Author(s) : Yuki M , Oshima K , Suda W , Kitahara M , Kitamura K , Iida T , Hattori M , Ohkuma M
Ref : Genome Announc , 2 : , 2014
Abstract : Here, we report the draft genome sequences of two type strains of Lactobacillus, Lactobacillus farraginis JCM 14108(T) and Lactobacillus composti JCM 14202(T), isolated from the compost of distilled shochu residue. Their genome information will be useful for studies of ecological and physiological functions of these Lactobacillus species.
ESTHER : Yuki_2014_Genome.Announc_2_e00257
PubMedSearch : Yuki_2014_Genome.Announc_2_e00257
PubMedID: 24675866
Gene_locus related to this paper: 9laco-x0pb49 , 9laco-x0psi7

Title : Draft Genome Sequence of Clostridium straminisolvens Strain JCM 21531T, Isolated from a Cellulose-Degrading Bacterial Community - Yuki_2014_Genome.Announc_2_e00110
Author(s) : Yuki M , Oshima K , Suda W , Sakamoto M , Kitamura K , Iida T , Hattori M , Ohkuma M
Ref : Genome Announc , 2 : , 2014
Abstract : Here, we report the draft genome sequence of a fibrolytic bacterium, Clostridium straminisolvens JCM 21531(T), isolated from a cellulose-degrading bacterial community. The genome information of this strain will be useful for studies on the degradation enzymes and functional interactions with other members in the community.
ESTHER : Yuki_2014_Genome.Announc_2_e00110
PubMedSearch : Yuki_2014_Genome.Announc_2_e00110
PubMedID: 24558248
Gene_locus related to this paper: 9firm-w4vb95 , 9firm-w4v5w9

Title : Genome evolution and plasticity of Serratia marcescens, an important multidrug-resistant nosocomial pathogen - Iguchi_2014_Genome.Biol.Evol_6_2096
Author(s) : Iguchi A , Nagaya Y , Pradel E , Ooka T , Ogura Y , Katsura K , Kurokawa K , Oshima K , Hattori M , Parkhill J , Sebaihia M , Coulthurst SJ , Gotoh N , Thomson NR , Ewbank JJ , Hayashi T
Ref : Genome Biol Evol , 6 :2096 , 2014
Abstract : Serratia marcescens is an important nosocomial pathogen that can cause an array of infections, most notably of the urinary tract and bloodstream. Naturally, it is found in many environmental niches, and is capable of infecting plants and animals. The emergence and spread of multidrug-resistant strains producing extended-spectrum or metallo beta-lactamases now pose a threat to public health worldwide. Here we report the complete genome sequences of two carefully selected S. marcescens strains, a multidrug-resistant clinical isolate (strain SM39) and an insect isolate (strain Db11). Our comparative analyses reveal the core genome of S. marcescens and define the potential metabolic capacity, virulence, and multidrug resistance of this species. We show a remarkable intraspecies genetic diversity, both at the sequence level and with regards genome flexibility, which may reflect the diversity of niches inhabited by members of this species. A broader analysis with other Serratia species identifies a set of approximately 3,000 genes that characterize the genus. Within this apparent genetic diversity, we identified many genes implicated in the high virulence potential and antibiotic resistance of SM39, including the metallo beta-lactamase and multiple other drug resistance determinants carried on plasmid pSMC1. We further show that pSMC1 is most closely related to plasmids circulating in Pseudomonas species. Our data will provide a valuable basis for future studies on S. marcescens and new insights into the genetic mechanisms that underlie the emergence of pathogens highly resistant to multiple antimicrobial agents.
ESTHER : Iguchi_2014_Genome.Biol.Evol_6_2096
PubMedSearch : Iguchi_2014_Genome.Biol.Evol_6_2096
PubMedID: 25070509
Gene_locus related to this paper: serma-a0a031rry9 , serma-a0a0a5sc46

Title : Draft Genome Sequences of Vibrio sp. Strains Isolated from Tetrodotoxin-Bearing Scavenging Gastropod - Kudo_2014_Genome.Announc_2_e00623
Author(s) : Kudo T , Kawauchi A , Nakahara T , Zhang X , Taniyama S , Takatani T , Arakawa O , Oshima K , Suda W , Kitamura K , Iida T , Iino T , Inoue T , Hongoh Y , Hattori M , Ohkuma M
Ref : Genome Announc , 2 : , 2014
Abstract : Vibrio sp. strains JCM 18905 and JCM 19053 were isolated from a tetrodotoxin (TTX)-bearing scavenging gastropod, and Vibrio sp. strain JCM 18904 was isolated from a sea cucumber. All these are closely related to Vibrio alginolyticus. Their comparative genome information is useful for studies of TTX production in bacteria.
ESTHER : Kudo_2014_Genome.Announc_2_e00623
PubMedSearch : Kudo_2014_Genome.Announc_2_e00623
PubMedID: 24948773

Title : Draft Genome Sequences of Marinobacter similis A3d10T and Marinobacter salarius R9SW1T - Ivanova_2014_Genome.Announc_2_e00442
Author(s) : Ivanova EP , Ng HJ , Webb HK , Feng G , Oshima K , Hattori M , Ohkuma M , Sergeev AF , Mikhailov VV , Crawford RJ , Sawabe T
Ref : Genome Announc , 2 : , 2014
Abstract : Here, we present the draft genomes of Marinobacter similis A3d10(T), a potential plastic biodegrader, and Marinobacter salarius R9SW1(T), isolated from radioactive waters. This genomic information will contribute information on the genetic basis of the metabolic pathways for the degradation of both plastic and radionuclides.
ESTHER : Ivanova_2014_Genome.Announc_2_e00442
PubMedSearch : Ivanova_2014_Genome.Announc_2_e00442
PubMedID: 24855296
Gene_locus related to this paper: 9alte-a6f367

Title : Draft Genome Sequence of the Alkaliphilic and Xylanolytic Paenibacillus sp. Strain JCM 10914, Isolated from the Gut of a Soil-Feeding Termite - Ohkuma_2014_Genome.Announc_2_e01144
Author(s) : Ohkuma M , Yuki M , Oshima K , Suda W , Oshida Y , Kitamura K , Iida T , Hattori M
Ref : Genome Announc , 2 :e01144 , 2014
Abstract : Panibacillus sp. strain JCM 10914 is a xylanolytic alkaliphile isolated from the gut of a soil-feeding termite. Its draft genome sequence revealed various genes for hydrolytic enzymes and will facilitate studies on adaptation to the highly alkaline gut environment and its role in digesting soil organic matter in the gut.
ESTHER : Ohkuma_2014_Genome.Announc_2_e01144
PubMedSearch : Ohkuma_2014_Genome.Announc_2_e01144
PubMedID: 24459258
Gene_locus related to this paper: 9bacl-v9gd19 , 9bacl-v9g8w0

Title : Draft Genome Sequences of Three Strains of Bacteroides pyogenes Isolated from a Cat and Swine - Sakamoto_2014_Genome.Announc_2_e01242
Author(s) : Sakamoto M , Oshima K , Suda W , Kitamura K , Iida T , Hattori M , Ohkuma M
Ref : Genome Announc , 2 : , 2014
Abstract : Here, we report the draft genome sequences of Bacteroides pyogenes JCM 6294(T), JCM 6292, and JCM 10003, which were isolated from a cat and swine and were recently classified into a single species, B. pyogenes. Comparative analyses of these genomes revealed the diversification of B. pyogenes strains isolated from different animals.
ESTHER : Sakamoto_2014_Genome.Announc_2_e01242
PubMedSearch : Sakamoto_2014_Genome.Announc_2_e01242
PubMedID: 24482517
Gene_locus related to this paper: 9bace-w4pec1 , 9bace-w4paz4

Title : Draft Genome Sequences of Psychrobacter Strains JCM 18900, JCM 18901, JCM 18902, and JCM 18903, Isolated Preferentially from Frozen Aquatic Organisms - Kudo_2014_Genome.Announc_2_e00280
Author(s) : Kudo T , Kidera A , Kida M , Kawauchi A , Shimizu R , Nakahara T , Zhang X , Yamada A , Amano M , Hamada Y , Taniyama S , Arakawa O , Yoshida A , Oshima K , Suda W , Kuwahara H , Nogi Y , Kitamura K , Yuki M , Iida T , Moriya S , Inoue T , Hongoh Y , Hattori M , Ohkuma M
Ref : Genome Announc , 2 :e00280 , 2014
Abstract : Four Psychrobacter strains, JCM 18900, JCM 18901, JCM 18902, and JCM 18903, related to either Psychrobacter nivimaris or Psychrobacter cibarius, were isolated from frozen marine animals. The genome information of these four strains will be useful for studies of their physiology and adaptation properties to frozen conditions.
ESTHER : Kudo_2014_Genome.Announc_2_e00280
PubMedSearch : Kudo_2014_Genome.Announc_2_e00280
PubMedID: 24699965
Gene_locus related to this paper: 9gamm-x0q9t6 , 9gamm-x0r0h9 , 9gamm-x0rb73 , 9gamm-x0qpg3 , 9gamm-x0rx94 , 9gamm-x0rpe0

Title : Draft Genome Sequence of Bacteroides reticulotermitis Strain JCM 10512T, Isolated from the Gut of a Termite - Yuki_2014_Genome.Announc_2_e00072
Author(s) : Yuki M , Oshima K , Suda W , Sakamoto M , Iida T , Hattori M , Ohkuma M
Ref : Genome Announc , 2 : , 2014
Abstract : Here we report the draft genome sequence of Bacteroides reticulotermitis strain JCM 10512(T), a xylanolytic and cellulolytic bacterium isolated from the gut of a wood-feeding termite. The genome information will facilitate the study of this strain for biomass degradation and adaptation to the gut environment.
ESTHER : Yuki_2014_Genome.Announc_2_e00072
PubMedSearch : Yuki_2014_Genome.Announc_2_e00072
PubMedID: 24526645
Gene_locus related to this paper: 9bace-w4uxt1 , 9bace-w4uy17 , 9bace-w4uuy7

Title : Genomic analysis by deep sequencing of the probiotic Lactobacillus brevis KB290 harboring nine plasmids reveals genomic stability - Fukao_2013_PLoS.One_8_e60521
Author(s) : Fukao M , Oshima K , Morita H , Toh H , Suda W , Kim SW , Suzuki S , Yakabe T , Hattori M , Yajima N
Ref : PLoS ONE , 8 :e60521 , 2013
Abstract : We determined the complete genome sequence of Lactobacillus brevis KB290, a probiotic lactic acid bacterium isolated from a traditional Japanese fermented vegetable. The genome contained a 2,395,134-bp chromosome that housed 2,391 protein-coding genes and nine plasmids that together accounted for 191 protein-coding genes. KB290 contained no virulence factor genes, and several genes related to presumptive cell wall-associated polysaccharide biosynthesis and the stress response were present in L. brevis KB290 but not in the closely related L. brevis ATCC 367. Plasmid-curing experiments revealed that the presence of plasmid pKB290-1 was essential for the strain's gastrointestinal tract tolerance and tendency to aggregate. Using next-generation deep sequencing of current and 18-year-old stock strains to detect low frequency variants, we evaluated genome stability. Deep sequencing of four periodic KB290 culture stocks with more than 1,000-fold coverage revealed 3 mutation sites and 37 minority variation sites, indicating long-term stability and providing a useful method for assessing the stability of industrial bacteria at the nucleotide level.
ESTHER : Fukao_2013_PLoS.One_8_e60521
PubMedSearch : Fukao_2013_PLoS.One_8_e60521
PubMedID: 23544154
Gene_locus related to this paper: lacba-q03sl1

Title : Genomic adaptation of the Lactobacillus casei group - Toh_2013_PLoS.One_8_e75073
Author(s) : Toh H , Oshima K , Nakano A , Takahata M , Murakami M , Takaki T , Nishiyama H , Igimi S , Hattori M , Morita H
Ref : PLoS ONE , 8 :e75073 , 2013
Abstract : Lactobacillus casei, L. paracasei, and L. rhamnosus form a closely related taxonomic group (Lactobacillus casei group) within the facultatively heterofermentative lactobacilli. Here, we report the complete genome sequences of L. paracasei JCM 8130 and L. casei ATCC 393, and the draft genome sequence of L. paracasei COM0101, all of which were isolated from daily products. Furthermore, we re-annotated the genome of L. rhamnosus ATCC 53103 (also known as L. rhamnosus GG), which we have previously reported. We confirmed that ATCC 393 is distinct from other strains previously described as L. paracasei. The core genome of 10 completely sequenced strains of the L. casei group comprised 1,682 protein-coding genes. Although extensive genome-wide synteny was found among the L. casei group, the genomes of ATCC 53103, JCM 8130, and ATCC 393 contained genomic islands compared with L. paracasei ATCC 334. Several genomic islands, including carbohydrate utilization gene clusters, were found at the same loci in the chromosomes of the L. casei group. The spaCBA pilus gene cluster, which was first identified in GG, was also found in other strains of the L. casei group, but several L. paracasei strains including COM0101 contained truncated spaC gene. ATCC 53103 encoded a higher number of proteins involved in carbohydrate utilization compared with intestinal lactobacilli, and extracellular adhesion proteins, several of which are absent in other strains of the L. casei group. In addition to previously fully sequenced L. rhamnosus and L. paracasei strains, the complete genome sequences of L. casei will provide valuable insights into the evolution of the L. casei group.
ESTHER : Toh_2013_PLoS.One_8_e75073
PubMedSearch : Toh_2013_PLoS.One_8_e75073
PubMedID: 24116025
Gene_locus related to this paper: lacc3-q03b36 , laccb-b3wcx2 , lacrh-pepr , lacca-b5qt93 , lacca-k0n1x0 , lacpa-s2ter8 , lacpa-s2rz88

Title : Genome analysis suggests that the soil oligotrophic bacterium Agromonas oligotrophica (Bradyrhizobium oligotrophicum) is a nitrogen-fixing symbiont of Aeschynomene indica - Okubo_2013_Appl.Environ.Microbiol_79_2542
Author(s) : Okubo T , Fukushima S , Itakura M , Oshima K , Longtonglang A , Teaumroong N , Mitsui H , Hattori M , Hattori R , Hattori T , Minamisawa K
Ref : Applied Environmental Microbiology , 79 :2542 , 2013
Abstract : Agromonas oligotrophica (Bradyrhizobium oligotrophicum) S58(T) is a nitrogen-fixing oligotrophic bacterium isolated from paddy field soil that is able to grow in extra-low-nutrient environments. Here, the complete genome sequence of S58 was determined. The S58 genome was found to comprise a circular chromosome of 8,264,165 bp with an average GC content of 65.1% lacking nodABC genes and the typical symbiosis island. The genome showed a high level of similarity to the genomes of Bradyrhizobium sp. ORS278 and Bradyrhizobium sp. BTAi1, including nitrogen fixation and photosynthesis gene clusters, which nodulate an aquatic legume plant, Aeschynomene indica, in a Nod factor-independent manner. Although nonsymbiotic (brady)rhizobia are significant components of rhizobial populations in soil, we found that most genes important for nodule development (ndv) and symbiotic nitrogen fixation (nif and fix) with A. indica were well conserved between the ORS278 and S58 genomes. Therefore, we performed inoculation experiments with five A. oligotrophica strains (S58, S42, S55, S72, and S80). Surprisingly, all five strains of A. oligotrophica formed effective nitrogen-fixing nodules on the roots and/or stems of A. indica, with differentiated bacteroids. Nonsymbiotic (brady)rhizobia are known to be significant components of rhizobial populations without a symbiosis island or symbiotic plasmids in soil, but the present results indicate that soil-dwelling A. oligotrophica generally possesses the ability to establish symbiosis with A. indica. Phylogenetic analyses suggest that Nod factor-independent symbiosis with A. indica is a common trait of nodABC- and symbiosis island-lacking strains within the members of the photosynthetic Bradyrhizobium clade, including A. oligotrophica.
ESTHER : Okubo_2013_Appl.Environ.Microbiol_79_2542
PubMedSearch : Okubo_2013_Appl.Environ.Microbiol_79_2542
PubMedID: 23396330
Gene_locus related to this paper: 9brad-m4zgf0 , 9brad-m4z0c0 , 9brad-m4za51 , 9brad-m4zdq9 , 9brad-m4zbd2

Title : Complete Genome Sequence of Burkholderia sp. Strain RPE64, Bacterial Symbiont of the Bean Bug Riptortus pedestris - Shibata_2013_Genome.Announc_1_e00441
Author(s) : Shibata TF , Maeda T , Nikoh N , Yamaguchi K , Oshima K , Hattori M , Nishiyama T , Hasebe M , Fukatsu T , Kikuchi Y , Shigenobu S
Ref : Genome Announc , 1 :e00441 , 2013
Abstract : We isolated Burkholderia symbiont strain RPE64 from the bean bug Riptortus pedestris. Analysis of the complete 6.96-Mb genome, which consists of three chromosomes and two plasmids, will facilitate further understanding of insect-microbe symbiosis and the development of pest-control technologies.
ESTHER : Shibata_2013_Genome.Announc_1_e00441
PubMedSearch : Shibata_2013_Genome.Announc_1_e00441
PubMedID: 23833137
Gene_locus related to this paper: 9burk-r4wy41 , 9burk-a0a069nn99 , 9burk-r4wtx8 , 9burk-r4wsg0 , 9burk-r4x084 , 9burk-r4wsp2

Title : Chromosome painting in silico in a bacterial species reveals fine population structure - Yahara_2013_Mol.Biol.Evol_30_1454
Author(s) : Yahara K , Furuta Y , Oshima K , Yoshida M , Azuma T , Hattori M , Uchiyama I , Kobayashi I
Ref : Molecular Biology Evolution , 30 :1454 , 2013
Abstract : Identifying population structure forms an important basis for genetic and evolutionary studies. Most current methods to identify population structure have limitations in analyzing haplotypes and recombination across the genome. Recently, a method of chromosome painting in silico has been developed to overcome these shortcomings and has been applied to multiple human genome sequences. This method detects the genome-wide transfer of DNA sequence chunks through homologous recombination. Here, we apply it to the frequently recombining bacterial species Helicobacter pylori that has infected Homo sapiens since their birth in Africa and shows wide phylogeographic divergence. Multiple complete genome sequences were analyzed including sequences from Okinawa, Japan, that we recently sequenced. The newer method revealed a finer population structure than revealed by a previous method that examines only MLST housekeeping genes or a phylogenetic network analysis of the core genome. Novel subgroups were found in Europe, Amerind, and East Asia groups. Examination of genetic flux showed some singleton strains to be hybrids of subgroups and revealed evident signs of population admixture in Africa, Europe, and parts of Asia. We expect this approach to further our understanding of intraspecific bacterial evolution by revealing population structure at a finer scale.
ESTHER : Yahara_2013_Mol.Biol.Evol_30_1454
PubMedSearch : Yahara_2013_Mol.Biol.Evol_30_1454
PubMedID: 23505045

Title : Complete genome sequence of Bradyrhizobium sp. S23321: insights into symbiosis evolution in soil oligotrophs - Okubo_2012_Microbes.Environ_27_306
Author(s) : Okubo T , Tsukui T , Maita H , Okamoto S , Oshima K , Fujisawa T , Saito A , Futamata H , Hattori R , Shimomura Y , Haruta S , Morimoto S , Wang Y , Sakai Y , Hattori M , Aizawa S , Nagashima KV , Masuda S , Hattori T , Yamashita A , Bao Z , Hayatsu M , Kajiya-Kanegae H , Yoshinaga I , Sakamoto K , Toyota K , Nakao M , Kohara M , Anda M , Niwa R , Jung-Hwan P , Sameshima-Saito R , Tokuda S , Yamamoto S , Yokoyama T , Akutsu T , Nakamura Y , Nakahira-Yanaka Y , Takada Hoshino Y , Hirakawa H , Mitsui H , Terasawa K , Itakura M , Sato S , Ikeda-Ohtsubo W , Sakakura N , Kaminuma E , Minamisawa K
Ref : Microbes Environ , 27 :306 , 2012
Abstract : Bradyrhizobium sp. S23321 is an oligotrophic bacterium isolated from paddy field soil. Although S23321 is phylogenetically close to Bradyrhizobium japonicum USDA110, a legume symbiont, it is unable to induce root nodules in siratro, a legume often used for testing Nod factor-dependent nodulation. The genome of S23321 is a single circular chromosome, 7,231,841 bp in length, with an average GC content of 64.3%. The genome contains 6,898 potential protein-encoding genes, one set of rRNA genes, and 45 tRNA genes. Comparison of the genome structure between S23321 and USDA110 showed strong colinearity; however, the symbiosis islands present in USDA110 were absent in S23321, whose genome lacked a chaperonin gene cluster (groELS3) for symbiosis regulation found in USDA110. A comparison of sequences around the tRNA-Val gene strongly suggested that S23321 contains an ancestral-type genome that precedes the acquisition of a symbiosis island by horizontal gene transfer. Although S23321 contains a nif (nitrogen fixation) gene cluster, the organization, homology, and phylogeny of the genes in this cluster were more similar to those of photosynthetic bradyrhizobia ORS278 and BTAi1 than to those on the symbiosis island of USDA110. In addition, we found genes encoding a complete photosynthetic system, many ABC transporters for amino acids and oligopeptides, two types (polar and lateral) of flagella, multiple respiratory chains, and a system for lignin monomer catabolism in the S23321 genome. These features suggest that S23321 is able to adapt to a wide range of environments, probably including low-nutrient conditions, with multiple survival strategies in soil and rhizosphere.
ESTHER : Okubo_2012_Microbes.Environ_27_306
PubMedSearch : Okubo_2012_Microbes.Environ_27_306
PubMedID: 22452844
Gene_locus related to this paper: 9brad-i0g2u8 , 9brad-i0gf89 , braja-pcaD , 9brad-i0fzh8 , 9brad-i0gfv2 , 9brad-i0g2y4

Title : Complete genome sequence of Bacillus cereus NC7401, which produces high levels of the emetic toxin cereulide - Takeno_2012_J.Bacteriol_194_4767
Author(s) : Takeno A , Okamoto A , Tori K , Oshima K , Hirakawa H , Toh H , Agata N , Yamada K , Ogasawara N , Hayashi T , Shimizu T , Kuhara S , Hattori M , Ohta M
Ref : Journal of Bacteriology , 194 :4767 , 2012
Abstract : We report the complete and annotated genome sequence of Bacillus cereus NC7401, a representative of the strain group that causes emetic-type food poisoning. The emetic toxin, cereulide, is produced by a nonribosomal protein synthesis (NRPS) system that is encoded by a gene cluster on a large resident plasmid, pNCcld.
ESTHER : Takeno_2012_J.Bacteriol_194_4767
PubMedSearch : Takeno_2012_J.Bacteriol_194_4767
PubMedID: 22887669
Gene_locus related to this paper: bacce-c2zyv1

Title : Complete genome sequence of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of L-lactic acid - Kato_2012_J.Bacteriol_194_2102
Author(s) : Kato H , Shiwa Y , Oshima K , Machii M , Araya-Kojima T , Zendo T , Shimizu-Kadota M , Hattori M , Sonomoto K , Yoshikawa H
Ref : Journal of Bacteriology , 194 :2102 , 2012
Abstract : We report the complete genome sequence of Lactococcus lactis IO-1 (= JCM7638). It is a nondairy lactic acid bacterium, produces nisin Z, ferments xylose, and produces predominantly L-lactic acid at high xylose concentrations. From ortholog analysis with other five L. lactis strains, IO-1 was identified as L. lactis subsp. lactis.
ESTHER : Kato_2012_J.Bacteriol_194_2102
PubMedSearch : Kato_2012_J.Bacteriol_194_2102
PubMedID: 22461545
Gene_locus related to this paper: lacla-menX

Title : Complete genome sequences of rat and mouse segmented filamentous bacteria, a potent inducer of th17 cell differentiation - Prakash_2011_Cell.Host.Microbe_10_273
Author(s) : Prakash T , Oshima K , Morita H , Fukuda S , Imaoka A , Kumar N , Sharma VK , Kim SW , Takahashi M , Saitou N , Taylor TD , Ohno H , Umesaki Y , Hattori M
Ref : Cell Host Microbe , 10 :273 , 2011
Abstract : Segmented filamentous bacteria (SFB) are noncultivable commensals inhabiting the gut of various vertebrate species and have been shown to induce Th17 cells in mice. We present the complete genome sequences of both rat and mouse SFB isolated from SFB-monocolonized hosts. The rat and mouse SFB genomes each harbor a single circular chromosome of 1.52 and 1.59 Mb encoding 1346 and 1420 protein-coding genes, respectively. The overall nucleotide identity between the two genomes is 86%, and the substitution rate was estimated to be similar to that of the free-living E. coli. SFB genomes encode typical genes for anaerobic fermentation and spore and flagella formation, but lack most of the amino acid biosynthesis enzymes, reminiscent of pathogenic Clostridia, exhibiting large dependency on the host. However, SFB lack most of the clostridial virulence-related genes. Comparative analysis with clostridial genomes suggested possible mechanisms for host responses and specific adaptations in the intestine.
ESTHER : Prakash_2011_Cell.Host.Microbe_10_273
PubMedSearch : Prakash_2011_Cell.Host.Microbe_10_273
PubMedID: 21925114
Gene_locus related to this paper: 9clot-g2ifk0

Title : Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae - Morita_2011_PLoS.One_6_e23184
Author(s) : Morita H , Toh H , Oshima K , Yoshizaki M , Kawanishi M , Nakaya K , Suzuki T , Miyauchi E , Ishii Y , Tanabe S , Murakami M , Hattori M
Ref : PLoS ONE , 6 :e23184 , 2011
Abstract : Lactococcus garvieae causes fatal haemorrhagic septicaemia in fish such as yellowtail. The comparative analysis of genomes of a virulent strain Lg2 and a non-virulent strain ATCC 49156 of L. garvieae revealed that the two strains shared a high degree of sequence identity, but Lg2 had a 16.5-kb capsule gene cluster that is absent in ATCC 49156. The capsule gene cluster was composed of 15 genes, of which eight genes are highly conserved with those in exopolysaccharide biosynthesis gene cluster often found in Lactococcus lactis strains. Sequence analysis of the capsule gene cluster in the less virulent strain L. garvieae Lg2-S, Lg2-derived strain, showed that two conserved genes were disrupted by a single base pair deletion, respectively. These results strongly suggest that the capsule is crucial for virulence of Lg2. The capsule gene cluster of Lg2 may be a genomic island from several features such as the presence of insertion sequences flanked on both ends, different GC content from the chromosomal average, integration into the locus syntenic to other lactococcal genome sequences, and distribution in human gut microbiomes. The analysis also predicted other potential virulence factors such as haemolysin. The present study provides new insights into understanding of the virulence mechanisms of L. garvieae in fish.
ESTHER : Morita_2011_PLoS.One_6_e23184
PubMedSearch : Morita_2011_PLoS.One_6_e23184
PubMedID: 21829716
Gene_locus related to this paper: lacgt-f9v7p0

Title : Complete genomic sequence of the O-desmethylangolensin-producing bacterium Clostridium rRNA cluster XIVa strain SY8519, isolated from adult human intestine - Yokoyama_2011_J.Bacteriol_193_5568
Author(s) : Yokoyama S , Oshima K , Nomura I , Hattori M , Suzuki T
Ref : Journal of Bacteriology , 193 :5568 , 2011
Abstract : The O-desmethylangolensin-producing Clostridium rRNA cluster XIVa strain SY8519 was isolated from the intestinal flora of a healthy human as a key isoflavonoid-metabolizing bacterium. Here, we report the finished and annotated genomic sequence of this organism.
ESTHER : Yokoyama_2011_J.Bacteriol_193_5568
PubMedSearch : Yokoyama_2011_J.Bacteriol_193_5568
PubMedID: 21914882
Gene_locus related to this paper: closs-f7v5q6 , closs-f7v6v0

Title : Complete genomic sequence of the equol-producing bacterium Eggerthella sp. strain YY7918, isolated from adult human intestine - Yokoyama_2011_J.Bacteriol_193_5570
Author(s) : Yokoyama S , Oshima K , Nomura I , Hattori M , Suzuki T
Ref : Journal of Bacteriology , 193 :5570 , 2011
Abstract : Eggerthella sp. strain YY7918 was isolated from the intestinal flora of a healthy human. It metabolizes daidzein (a soybean isoflavonoid) and produces S-equol, which has stronger estrogenic activities than daidzein. Here, we report the finished and annotated genomic sequence of this organism.
ESTHER : Yokoyama_2011_J.Bacteriol_193_5570
PubMedSearch : Yokoyama_2011_J.Bacteriol_193_5570
PubMedID: 21914883
Gene_locus related to this paper: eegsy-f7uz47

Title : Complete genome sequences of Arcobacter butzleri ED-1 and Arcobacter sp. strain L, both isolated from a microbial fuel cell - Toh_2011_J.Bacteriol_193_6411
Author(s) : Toh H , Sharma VK , Oshima K , Kondo S , Hattori M , Ward FB , Free A , Taylor TD
Ref : Journal of Bacteriology , 193 :6411 , 2011
Abstract : Arcobacter butzleri strain ED-1 is an exoelectrogenic epsilonproteobacterium isolated from the anode biofilm of a microbial fuel cell. Arcobacter sp. strain L dominates the liquid phase of the same fuel cell. Here we report the finished and annotated genome sequences of these organisms.
ESTHER : Toh_2011_J.Bacteriol_193_6411
PubMedSearch : Toh_2011_J.Bacteriol_193_6411
PubMedID: 22038970
Gene_locus related to this paper: 9prot-e6l4v2 , 9prot-g2hv40 , 9prot-a0a0g9kwp1 , 9prot-g2hwt3

Title : Bifidobacteria can protect from enteropathogenic infection through production of acetate - Fukuda_2011_Nature_469_543
Author(s) : Fukuda S , Toh H , Hase K , Oshima K , Nakanishi Y , Yoshimura K , Tobe T , Clarke JM , Topping DL , Suzuki T , Taylor TD , Itoh K , Kikuchi J , Morita H , Hattori M , Ohno H
Ref : Nature , 469 :543 , 2011
Abstract : The human gut is colonized with a wide variety of microorganisms, including species, such as those belonging to the bacterial genus Bifidobacterium, that have beneficial effects on human physiology and pathology. Among the most distinctive benefits of bifidobacteria are modulation of host defence responses and protection against infectious diseases. Nevertheless, the molecular mechanisms underlying these effects have barely been elucidated. To investigate these mechanisms, we used mice associated with certain bifidobacterial strains and a simplified model of lethal infection with enterohaemorrhagic Escherichia coli O157:H7, together with an integrated 'omics' approach. Here we show that genes encoding an ATP-binding-cassette-type carbohydrate transporter present in certain bifidobacteria contribute to protecting mice against death induced by E. coli O157:H7. We found that this effect can be attributed, at least in part, to increased production of acetate and that translocation of the E. coli O157:H7 Shiga toxin from the gut lumen to the blood was inhibited. We propose that acetate produced by protective bifidobacteria improves intestinal defence mediated by epithelial cells and thereby protects the host against lethal infection.
ESTHER : Fukuda_2011_Nature_469_543
PubMedSearch : Fukuda_2011_Nature_469_543
PubMedID: 21270894
Gene_locus related to this paper: bifli-c2gxu7 , biflo-BL0073 , biflo-BL0336 , biflo-BL0581 , biflo-BL0582 , biflo-BL0682 , biflo-BL0787 , biflo-BL0807 , biflo-BL1109 , biflo-BL1514 , biflo-PAP , biflo-PTRB , bifln-c2gtr2

Title : Complete genome sequence of the denitrifying and N(2)O-reducing bacterium Pseudogulbenkiania sp. strain NH8B - Ishii_2011_J.Bacteriol_193_6395
Author(s) : Ishii S , Tago K , Nishizawa T , Oshima K , Hattori M , Senoo K
Ref : Journal of Bacteriology , 193 :6395 , 2011
Abstract : Pseudogulbenkiania sp. strain NH8B is a Neisseriales bacterium isolated from an agricultural field. This strain has strong denitrification and N(2)O reduction activities. Here, we report the finished and annotated genome sequence of this organism.
ESTHER : Ishii_2011_J.Bacteriol_193_6395
PubMedSearch : Ishii_2011_J.Bacteriol_193_6395
PubMedID: 22038961
Gene_locus related to this paper: pseul-g2j1k1 , pseul-g2iue3 , pseul-g2j5g5

Title : Birth and death of genes linked to chromosomal inversion - Furuta_2011_Proc.Natl.Acad.Sci.U.S.A_108_1501
Author(s) : Furuta Y , Kawai M , Yahara K , Takahashi N , Handa N , Tsuru T , Oshima K , Yoshida M , Azuma T , Hattori M , Uchiyama I , Kobayashi I
Ref : Proc Natl Acad Sci U S A , 108 :1501 , 2011
Abstract : The birth and death of genes is central to adaptive evolution, yet the underlying genome dynamics remain elusive. The availability of closely related complete genome sequences helps to follow changes in gene contents and clarify their relationship to overall genome organization. Helicobacter pylori, bacteria in our stomach, are known for their extreme genome plasticity through mutation and recombination and will make a good target for such an analysis. In comparing their complete genome sequences, we found that gain and loss of genes (loci) for outer membrane proteins, which mediate host interaction, occurred at breakpoints of chromosomal inversions. Sequence comparison there revealed a unique mechanism of DNA duplication: DNA duplication associated with inversion. In this process, a DNA segment at one chromosomal locus is copied and inserted, in an inverted orientation, into a distant locus on the same chromosome, while the entire region between these two loci is also inverted. Recognition of this and three more inversion modes, which occur through reciprocal recombination between long or short sequence similarity or adjacent to a mobile element, allowed reconstruction of synteny evolution through inversion events in this species. These results will guide the interpretation of extensive DNA sequencing results for understanding long- and short-term genome evolution in various organisms and in cancer cells.
ESTHER : Furuta_2011_Proc.Natl.Acad.Sci.U.S.A_108_1501
PubMedSearch : Furuta_2011_Proc.Natl.Acad.Sci.U.S.A_108_1501
PubMedID: 21212362

Title : Evolution in an oncogenic bacterial species with extreme genome plasticity: Helicobacter pylori East Asian genomes - Kawai_2011_BMC.Microbiol_11_104
Author(s) : Kawai M , Furuta Y , Yahara K , Tsuru T , Oshima K , Handa N , Takahashi N , Yoshida M , Azuma T , Hattori M , Uchiyama I , Kobayashi I
Ref : BMC Microbiol , 11 :104 , 2011
Abstract : BACKGROUND: The genome of Helicobacter pylori, an oncogenic bacterium in the human stomach, rapidly evolves and shows wide geographical divergence. The high incidence of stomach cancer in East Asia might be related to bacterial genotype. We used newly developed comparative methods to follow the evolution of East Asian H. pylori genomes using 20 complete genome sequences from Japanese, Korean, Amerind, European, and West African strains.
RESULTS: A phylogenetic tree of concatenated well-defined core genes supported divergence of the East Asian lineage (hspEAsia; Japanese and Korean) from the European lineage ancestor, and then from the Amerind lineage ancestor. Phylogenetic profiling revealed a large difference in the repertoire of outer membrane proteins (including oipA, hopMN, babABC, sabAB and vacA-2) through gene loss, gain, and mutation. All known functions associated with molybdenum, a rare element essential to nearly all organisms that catalyzes two-electron-transfer oxidation-reduction reactions, appeared to be inactivated. Two pathways linking acetyl~CoA and acetate appeared intact in some Japanese strains. Phylogenetic analysis revealed greater divergence between the East Asian (hspEAsia) and the European (hpEurope) genomes in proteins in host interaction, specifically virulence factors (tipalpha), outer membrane proteins, and lipopolysaccharide synthesis (human Lewis antigen mimicry) enzymes. Divergence was also seen in proteins in electron transfer and translation fidelity (miaA, tilS), a DNA recombinase/exonuclease that recognizes genome identity (addA), and DNA/RNA hybrid nucleases (rnhAB). Positively selected amino acid changes between hspEAsia and hpEurope were mapped to products of cagA, vacA, homC (outer membrane protein), sotB (sugar transport), and a translation fidelity factor (miaA). Large divergence was seen in genes related to antibiotics: frxA (metronidazole resistance), def (peptide deformylase, drug target), and ftsA (actin-like, drug target).
CONCLUSIONS: These results demonstrate dramatic genome evolution within a species, especially in likely host interaction genes. The East Asian strains appear to differ greatly from the European strains in electron transfer and redox reactions. These findings also suggest a model of adaptive evolution through proteome diversification and selection through modulation of translational fidelity. The results define H. pylori East Asian lineages and provide essential information for understanding their pathogenesis and designing drugs and therapies that target them.
ESTHER : Kawai_2011_BMC.Microbiol_11_104
PubMedSearch : Kawai_2011_BMC.Microbiol_11_104
PubMedID: 21575176

Title : Complete genome sequence of the wild-type commensal Escherichia coli strain SE15, belonging to phylogenetic group B2 - Toh_2010_J.Bacteriol_192_1165
Author(s) : Toh H , Oshima K , Toyoda A , Ogura Y , Ooka T , Sasamoto H , Park SH , Iyoda S , Kurokawa K , Morita H , Itoh K , Taylor TD , Hayashi T , Hattori M
Ref : Journal of Bacteriology , 192 :1165 , 2010
Abstract : Escherichia coli SE15 (O150:H5) is a human commensal bacterium recently isolated from feces of a healthy adult and classified into E. coli phylogenetic group B2, which includes the majority of extraintestinal pathogenic E. coli. Here, we report the finished and annotated genome sequence of this organism.
ESTHER : Toh_2010_J.Bacteriol_192_1165
PubMedSearch : Toh_2010_J.Bacteriol_192_1165
PubMedID: 20008064
Gene_locus related to this paper: ecoli-Aes , ecoli-rutD , ecoli-bioh , ecoli-C0410 , ecoli-C2429 , ecoli-C4836 , ecoli-dlhh , ecoli-entf , ecoli-fes , ecoli-mhpc , ecoli-pldb , ecoli-ptrb , ecoli-yafa , ecoli-yaim , ecoli-ybff , ecoli-ycfp , ecoli-ycjy , ecoli-yeiG , ecoli-YFBB , ecoli-yghX , ecoli-yhet , ecoli-yiel , ecoli-yjfp , ecoli-ypfh , ecoli-ypt1 , ecoli-yqia , ecoli-YfhR , ecos5-d2nhh3 , yerpe-YBTT , ecolx-f4suw9

Title : Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli - Ogura_2009_Proc.Natl.Acad.Sci.U.S.A_106_17939
Author(s) : Ogura Y , Ooka T , Iguchi A , Toh H , Asadulghani M , Oshima K , Kodama T , Abe H , Nakayama K , Kurokawa K , Tobe T , Hattori M , Hayashi T
Ref : Proc Natl Acad Sci U S A , 106 :17939 , 2009
Abstract : Among the various pathogenic Escherichia coli strains, enterohemorrhagic E. coli (EHEC) is the most devastating. Although serotype O157:H7 strains are the most prevalent, strains of different serotypes also possess similar pathogenic potential. Here, we present the results of a genomic comparison between EHECs of serotype O157, O26, O111, and O103, as well as 21 other, fully sequenced E. coli/Shigella strains. All EHECs have much larger genomes (5.5-5.9 Mb) than the other strains and contain surprisingly large numbers of prophages and integrative elements (IEs). The gene contents of the 4 EHECs do not follow the phylogenetic relationships of the strains, and they share virulence genes for Shiga toxins and many other factors. We found many lambdoid phages, IEs, and virulence plasmids that carry the same or similar virulence genes but have distinct evolutionary histories, indicating that independent acquisition of these mobile genetic elements has driven the evolution of each EHEC. Particularly interesting is the evolution of the type III secretion system (T3SS). We found that the T3SS of EHECs is composed of genes that were introduced by 3 different types of genetic elements: an IE referred to as the locus of enterocyte effacement, which encodes a central part of the T3SS; SpLE3-like IEs; and lambdoid phages carrying numerous T3SS effector genes and other T3SS-related genes. Our data demonstrate how E. coli strains of different phylogenies can independently evolve into EHECs, providing unique insights into the mechanisms underlying the parallel evolution of complex virulence systems in bacteria.
ESTHER : Ogura_2009_Proc.Natl.Acad.Sci.U.S.A_106_17939
PubMedSearch : Ogura_2009_Proc.Natl.Acad.Sci.U.S.A_106_17939
PubMedID: 19815525
Gene_locus related to this paper: ecoli-Aes , ecoli-rutD , ecoli-bioh , ecoli-C2429 , ecoli-C4836 , ecoli-d7xp23 , ecoli-dlhh , ecoli-entf , ecoli-fes , ecoli-mhpc , ecoli-pldb , ecoli-ptrb , ecoli-yafa , ecoli-yaim , ecoli-ybff , ecoli-ycfp , ecoli-ycjy , ecoli-yeiG , ecoli-YFBB , ecoli-yghX , ecoli-yhet , ecoli-yiel , ecoli-yjfp , ecoli-YNBC , ecoli-ypfh , ecoli-ypt1 , ecoli-yqia , ecoli-Z1341 , ecoli-Z1930 , ecoli-Z2445 , ecoli-YfhR , yerpe-YBTT

Title : Complete genome sequence of the probiotic Lactobacillus rhamnosus ATCC 53103 - Morita_2009_J.Bacteriol_191_7630
Author(s) : Morita H , Toh H , Oshima K , Murakami M , Taylor TD , Igimi S , Hattori M
Ref : Journal of Bacteriology , 191 :7630 , 2009
Abstract : Lactobacillus rhamnosus is a facultatively heterofermentative lactic acid bacterium and is frequently isolated from human gastrointestinal mucosa of healthy individuals. L. rhamnosus ATCC 53103, isolated from a healthy human intestinal flora, is one of the most widely used and well-documented probiotics. Here, we report the finished and annotated genome sequence of this organism.
ESTHER : Morita_2009_J.Bacteriol_191_7630
PubMedSearch : Morita_2009_J.Bacteriol_191_7630
PubMedID: 19820099
Gene_locus related to this paper: lacrg-c7tei3 , lacrh-B2CZF3 , lacrh-b5qmk1 , lacrh-pepr , lacrh-pepx , lacrl-pip

Title : Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult - Oshima_2008_DNA.Res_15_375
Author(s) : Oshima K , Toh H , Ogura Y , Sasamoto H , Morita H , Park SH , Ooka T , Iyoda S , Taylor TD , Hayashi T , Itoh K , Hattori M
Ref : DNA Research , 15 :375 , 2008
Abstract : We sequenced and analyzed the genome of a commensal Escherichia coli (E. coli) strain SE11 (O152:H28) recently isolated from feces of a healthy adult and classified into E. coli phylogenetic group B1. SE11 harbored a 4.8 Mb chromosome encoding 4679 protein-coding genes and six plasmids encoding 323 protein-coding genes. None of the SE11 genes had sequence similarity to known genes encoding phage- and plasmid-borne virulence factors found in pathogenic E. coli strains. The comparative genome analysis with the laboratory strain K-12 MG1655 identified 62 poorly conserved genes between these two non-pathogenic strains and 1186 genes absent in MG1655. These genes in SE11 were mostly encoded in large insertion regions on the chromosome or in the plasmids, and were notably abundant in genes of fimbriae and autotransporters, which are cell surface appendages that largely contribute to the adherence ability of bacteria to host cells and bacterial conjugation. These data suggest that SE11 may have evolved to acquire and accumulate the functions advantageous for stable colonization of intestinal cells, and that the adhesion-associated functions are important for the commensality of E. coli in human gut habitat.
ESTHER : Oshima_2008_DNA.Res_15_375
PubMedSearch : Oshima_2008_DNA.Res_15_375
PubMedID: 18931093
Gene_locus related to this paper: ecoli-Aes , ecoli-rutD , ecoli-bioh , ecoli-C4836 , ecoli-dlhh , ecoli-entf , ecoli-fes , ecoli-mhpc , ecoli-pldb , ecoli-ptrb , ecoli-yafa , ecoli-yaim , ecoli-ybff , ecoli-ycfp , ecoli-ycjy , ecoli-yeiG , ecoli-YFBB , ecoli-yghX , ecoli-yhet , ecoli-yiel , ecoli-yjfp , ecoli-YNBC , ecoli-ypfh , ecoli-yqia , ecoli-Z1930 , ecoli-Z2445 , ecoli-YfhR

Title : Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production - Morita_2008_DNA.Res_15_151
Author(s) : Morita H , Toh H , Fukuda S , Horikawa H , Oshima K , Suzuki T , Murakami M , Hisamatsu S , Kato Y , Takizawa T , Fukuoka H , Yoshimura T , Itoh K , O'Sullivan DJ , McKay LL , Ohno H , Kikuchi J , Masaoka T , Hattori M
Ref : DNA Research , 15 :151 , 2008
Abstract : Lactobacillus reuteri is a heterofermentative lactic acid bacterium that naturally inhabits the gut of humans and other animals. The probiotic effects of L. reuteri have been proposed to be largely associated with the production of the broad-spectrum antimicrobial compound reuterin during anaerobic metabolism of glycerol. We determined the complete genome sequences of the reuterin-producing L. reuteri JCM 1112(T) and its closely related species Lactobacillus fermentum IFO 3956. Both are in the same phylogenetic group within the genus Lactobacillus. Comparative genome analysis revealed that L. reuteri JCM 1112(T) has a unique cluster of 58 genes for the biosynthesis of reuterin and cobalamin (vitamin B(12)). The 58-gene cluster has a lower GC content and is apparently inserted into the conserved region, suggesting that the cluster represents a genomic island acquired from an anomalous source. Two-dimensional nuclear magnetic resonance (2D-NMR) with (13)C(3)-glycerol demonstrated that L. reuteri JCM 1112(T) could convert glycerol to reuterin in vivo, substantiating the potential of L. reuteri JCM 1112(T) to produce reuterin in the intestine. Given that glycerol is shown to be naturally present in feces, the acquired ability to produce reuterin and cobalamin is an adaptive evolutionary response that likely contributes to the probiotic properties of L. reuteri.
ESTHER : Morita_2008_DNA.Res_15_151
PubMedSearch : Morita_2008_DNA.Res_15_151
PubMedID: 18487258
Gene_locus related to this paper: lacfe-c0wz89 , lacre-a5vi88 , lacre-b3xl59 , lacre-b3xl60 , lacre-b3xlh0 , lacre-b3xps7 , lacre-q4jle7 , lacre-q4jlf2 , lacre-q4jll5 , lacre-q6wu85 , lacrj-b2g622

Title : The Whole-genome sequencing of the obligate intracellular bacterium Orientia tsutsugamushi revealed massive gene amplification during reductive genome evolution - Nakayama_2008_DNA.Res_15_185
Author(s) : Nakayama K , Yamashita A , Kurokawa K , Morimoto T , Ogawa M , Fukuhara M , Urakami H , Ohnishi M , Uchiyama I , Ogura Y , Ooka T , Oshima K , Tamura A , Hattori M , Hayashi T
Ref : DNA Research , 15 :185 , 2008
Abstract : Scrub typhus ('Tsutsugamushi' disease in Japanese) is a mite-borne infectious disease. The causative agent is Orientia tsutsugamushi, an obligate intracellular bacterium belonging to the family Rickettsiaceae of the subdivision alpha-Proteobacteria. In this study, we determined the complete genome sequence of O. tsutsugamushi strain Ikeda, which comprises a single chromosome of 2 008 987 bp and contains 1967 protein coding sequences (CDSs). The chromosome is much larger than those of other members of Rickettsiaceae, and 46.7% of the sequence was occupied by repetitive sequences derived from an integrative and conjugative element, 10 types of transposable elements, and seven types of short repeats of unknown origins. The massive amplification and degradation of these elements have generated a huge number of repeated genes (1196 CDSs, categorized into 85 families), many of which are pseudogenes (766 CDSs), and also induced intensive genome shuffling. By comparing the gene content with those of other family members of Rickettsiacea, we identified the core gene set of the family Rickettsiaceae and found that, while much more extensive gene loss has taken place among the housekeeping genes of Orientia than those of Rickettsia, O. tsutsugamushi has acquired a large number of foreign genes. The O. tsutsugamushi genome sequence is thus a prominent example of the high plasticity of bacterial genomes, and provides the genetic basis for a better understanding of the biology of O. tsutsugamushi and the pathogenesis of 'Tsutsugamushi' disease.
ESTHER : Nakayama_2008_DNA.Res_15_185
PubMedSearch : Nakayama_2008_DNA.Res_15_185
PubMedID: 18508905
Gene_locus related to this paper: oriti-b3cqy7

Title : Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host - Toh_2006_Genome.Res_16_149
Author(s) : Toh H , Weiss BL , Perkin SA , Yamashita A , Oshima K , Hattori M , Aksoy S
Ref : Genome Res , 16 :149 , 2006
Abstract : Sodalis glossinidius is a maternally transmitted endosymbiont of tsetse flies (Glossina spp.), an insect of medical and veterinary significance. Analysis of the complete sequence of Sodalis' chromosome (4,171,146 bp, encoding 2,432 protein coding sequences) indicates a reduced coding capacity of 51%. Furthermore, the chromosome contains 972 pseudogenes, an inordinately high number compared with that of other bacterial species. A high proportion of these pseudogenes are homologs of known proteins that function either in defense or in the transport and metabolism of carbohydrates and inorganic ions, suggesting Sodalis' degenerative adaptations to the immunity and restricted nutritional status of the host. Sodalis possesses three chromosomal symbiosis regions (SSR): SSR-1, SSR-2, and SSR-3, with gene inventories similar to the Type-III secretion system (TTSS) ysa from Yersinia enterolitica and SPI-1 and SPI-2 from Salmonella, respectively. While core components of the needle structure have been conserved, some of the effectors and regulators typically associated with these systems in pathogenic microbes are modified or eliminated in Sodalis. Analysis of SSR-specific invA transcript abundance in Sodalis during host development indicates that the individual symbiosis regions may exhibit different temporal expression profiles. In addition, the Sodalis chromosome encodes a complete flagella structure, key components of which are expressed in immature host developmental stages. These features may be important for the transmission and establishment of symbiont infections in the intra-uterine progeny. The data suggest that Sodalis represents an evolutionary intermediate transitioning from a free-living to a mutualistic lifestyle.
ESTHER : Toh_2006_Genome.Res_16_149
PubMedSearch : Toh_2006_Genome.Res_16_149
PubMedID: 16365377
Gene_locus related to this paper: sodgm-q2nqh6 , sodgm-q2nqt2 , sodgm-q2nss6 , sodgm-q2nt34 , sodgm-q2nth7 , sodgm-q2nue4 , sodgm-q2nvf1 , sodgm-q2nwd0

Title : What influences the results in critical patients after cardiovascular surgery? - Ishikawa_2004_Asian.Cardiovasc.Thorac.Ann_12_250
Author(s) : Ishikawa S , Koyano T , Takahashi T , Sato Y , Hasegawa Y , Ohki S , Oshima K , Oki S , Kunimoto F , Morishita Y
Ref : Asian Cardiovasc Thorac Ann , 12 :250 , 2004
Abstract : The predictive factors of surgical outcome were evaluated in compromised patients following cardiovascular surgery. Of 608 patients undergoing cardiovascular surgery between 1991 and 1999, 55 stayed in the intensive care unit for 2 weeks or longer. The mean age of these 55 patients was 56 years. There were 35 survivors and 20 nonsurvivors. Postoperative respiratory failure and gastrointestinal complications were significantly more frequent in those who died. The survival rate was significantly higher in patients who had enteral feeding compared to those who did not (88% versus 43%). Serum cholinesterase and total cholesterol concentrations were higher in the survivors. It was concluded that postoperative respiratory and gastrointestinal conditions influenced the surgical outcome, and serum cholinesterase and total cholesterol concentrations were valuable predictors of survival.
ESTHER : Ishikawa_2004_Asian.Cardiovasc.Thorac.Ann_12_250
PubMedSearch : Ishikawa_2004_Asian.Cardiovasc.Thorac.Ann_12_250
PubMedID: 15353466

Title : Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V cholerae - Makino_2003_Lancet_361_743
Author(s) : Makino K , Oshima K , Kurokawa K , Yokoyama K , Uda T , Tagomori K , Iijima Y , Najima M , Nakano M , Yamashita A , Kubota Y , Kimura S , Yasunaga T , Honda T , Shinagawa H , Hattori M , Iida T
Ref : Lancet , 361 :743 , 2003
Abstract : Background Vibrio parahaemolyticus, a gram-negative marine bacterium, is a worldwide cause of food-borne gastroenteritis. V parahaemolyticus strains of a few specific serotypes, probably derived from a common clonal ancestor, have lately caused a pandemic of gastroenteritis. The organism is phylogenetically close to V cholerae, the causative agent of cholera. METHODS: The whole genome sequence of a clinical V parahaemolyticus strain RIMD2210633 was established by shotgun sequencing. The coding sequences were identified by use of Gambler and Glimmer programs. Comparative analysis with the V cholerae genome was undertaken with MUMmer. FINDINGS: The genome consisted of two circular chromosomes of 3288558 bp and 1877212 bp; it contained 4832 genes. Comparison of the V parahaemolyticus genome with that of V cholerae showed many rearrangements within and between the two chromosomes. Genes for the type III secretion system (TTSS) were identified in the genome of V parahaemolyticus; V cholerae does not have these genes. INTERPRETATION: The TTSS is a central virulence factor of diarrhoea-causing bacteria such as shigella, salmonella, and enteropathogenic Escherichia coli, which cause gastroenteritis by invading or intimately interacting with intestinal epithelial cells. Our results suggest that V parahaemolyticus and V cholerae use distinct mechanisms to establish infection. This finding explains clinical features of V parahaemolyticus infections, which commonly include inflammatory diarrhoea and in some cases systemic manifestations such as septicaemia, distinct from those of V cholerae infections, which are generally associated with non-inflammatory diarrhoea.
ESTHER : Makino_2003_Lancet_361_743
PubMedSearch : Makino_2003_Lancet_361_743
PubMedID: 12620739
Gene_locus related to this paper: vibpa-PHAC , vibpa-VP0148 , vibpa-VP0409 , vibpa-VP0429 , vibpa-VP0626 , vibpa-VP0693 , vibpa-VP0837 , vibpa-VP0869 , vibpa-VP0930 , vibpa-VP1025 , vibpa-VP1181 , vibpa-VP1677 , vibpa-VP1678 , vibpa-VP2790 , vibpa-VP2974 , vibpa-VPA0054 , vibpa-VPA0070 , vibpa-VPA0081 , vibpa-VPA0167 , vibpa-VPA0468 , vibpa-VPA0859 , vibpa-VPA0997 , vibpa-VPA1061 , vibpa-VPA1249 , vibpa-VPA1467 , vibpa-VPA1496 , vibpa-VPA1595 , vibpa-y674 , vibpa-y969

Title : Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia - Akman_2002_Nat.Genet_32_402
Author(s) : Akman L , Yamashita A , Watanabe H , Oshima K , Shiba T , Hattori M , Aksoy S
Ref : Nat Genet , 32 :402 , 2002
Abstract : Many insects that rely on a single food source throughout their developmental cycle harbor beneficial microbes that provide nutrients absent from their restricted diet. Tsetse flies, the vectors of African trypanosomes, feed exclusively on blood and rely on one such intracellular microbe for nutritional provisioning and fecundity. As a result of co-evolution with hosts over millions of years, these mutualists have lost the ability to survive outside the sheltered environment of their host insect cells. We present the complete annotated genome of Wigglesworthia glossinidia brevipalpis, which is composed of one chromosome of 697,724 base pairs (bp) and one small plasmid, called pWig1, of 5,200 bp. Genes involved in the biosynthesis of vitamin metabolites, apparently essential for host nutrition and fecundity, have been retained. Unexpectedly, this obligate's genome bears hallmarks of both parasitic and free-living microbes, and the gene encoding the important regulatory protein DnaA is absent.
ESTHER : Akman_2002_Nat.Genet_32_402
PubMedSearch : Akman_2002_Nat.Genet_32_402
PubMedID: 12219091
Gene_locus related to this paper: wigbr-BIOH

Title : The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans - Sasaki_2002_Nucleic.Acids.Res_30_5293
Author(s) : Sasaki Y , Ishikawa J , Yamashita A , Oshima K , Kenri T , Furuya K , Yoshino C , Horino A , Shiba T , Sasaki T , Hattori M
Ref : Nucleic Acids Research , 30 :5293 , 2002
Abstract : The complete genomic sequence of an intracellular bacterial pathogen, Mycoplasma penetrans HF-2 strain, was determined. The HF-2 genome consists of a 1 358 633 bp single circular chromosome containing 1038 predicted coding sequences (CDSs), one set of rRNA genes and 30 tRNA genes. Among the 1038 CDSs, 264 predicted proteins are common to the Mycoplasmataceae sequenced thus far and 463 are M.penetrans specific. The genome contains the two-component system but lacks the essential cellular gene, uridine kinase. The relatively large genome of M.penetrans HF-2 among mycoplasma species may be accounted for by both its rich core proteome and the presence of a number of paralog families corresponding to 25.4% of all CDSs. The largest paralog family is the p35 family, which encodes surface lipoproteins including the major antigen, P35. A total of 44 genes for p35 and p35 homologs were identified and 30 of them form one large cluster in the chromosome. The genetic tree of p35 paralogs suggests the occurrence of dynamic chromosomal rearrangement in paralog formation during evolution. Thus, M.penetrans HF-2 may have acquired diverse repertoires of antigenic variation-related genes to allow its persistent infection in humans.
ESTHER : Sasaki_2002_Nucleic.Acids.Res_30_5293
PubMedSearch : Sasaki_2002_Nucleic.Acids.Res_30_5293
PubMedID: 12466555
Gene_locus related to this paper: mycpe-MYPE3930 , mycpe-MYPE5460 , mycpe-MYPE8330 , mycpe-MYPE9300 , mycpe-q8eve6

Title : Whole genome sequencing of meticillin-resistant Staphylococcus aureus - Kuroda_2001_Lancet_357_1225
Author(s) : Kuroda M , Ohta T , Uchiyama I , Baba T , Yuzawa H , Kobayashi I , Cui L , Oguchi A , Aoki K , Nagai Y , Lian J , Ito T , Kanamori M , Matsumaru H , Maruyama A , Murakami H , Hosoyama A , Mizutani-Ui Y , Takahashi NK , Sawano T , Inoue R , Kaito C , Sekimizu K , Hirakawa H , Kuhara S , Goto S , Yabuzaki J , Kanehisa M , Yamashita A , Oshima K , Furuya K , Yoshino C , Shiba T , Hattori M , Ogasawara N , Hayashi H , Hiramatsu K
Ref : Lancet , 357 :1225 , 2001
Abstract : BACKGROUND: Staphylococcus aureus is one of the major causes of community-acquired and hospital-acquired infections. It produces numerous toxins including superantigens that cause unique disease entities such as toxic-shock syndrome and staphylococcal scarlet fever, and has acquired resistance to practically all antibiotics. Whole genome analysis is a necessary step towards future development of countermeasures against this organism.
METHODS: Whole genome sequences of two related S aureus strains (N315 and Mu50) were determined by shot-gun random sequencing. N315 is a meticillin-resistant S aureus (MRSA) strain isolated in 1982, and Mu50 is an MRSA strain with vancomycin resistance isolated in 1997. The open reading frames were identified by use of GAMBLER and GLIMMER programs, and annotation of each was done with a BLAST homology search, motif analysis, and protein localisation prediction. FINDINGS: The Staphylococcus genome was composed of a complex mixture of genes, many of which seem to have been acquired by lateral gene transfer. Most of the antibiotic resistance genes were carried either by plasmids or by mobile genetic elements including a unique resistance island. Three classes of new pathogenicity islands were identified in the genome: a toxic-shock-syndrome toxin island family, exotoxin islands, and enterotoxin islands. In the latter two pathogenicity islands, clusters of exotoxin and enterotoxin genes were found closely linked with other gene clusters encoding putative pathogenic factors. The analysis also identified 70 candidates for new virulence factors. INTERPRETATION: The remarkable ability of S aureus to acquire useful genes from various organisms was revealed through the observation of genome complexity and evidence of lateral gene transfer. Repeated duplication of genes encoding superantigens explains why S aureus is capable of infecting humans of diverse genetic backgrounds, eliciting severe immune reactions. Investigation of many newly identified gene products, including the 70 putative virulence factors, will greatly improve our understanding of the biology of staphylococci and the processes of infectious diseases caused by S aureus.
ESTHER : Kuroda_2001_Lancet_357_1225
PubMedSearch : Kuroda_2001_Lancet_357_1225
PubMedID: 11418146
Gene_locus related to this paper: staau-LIP , staau-lipas , staau-MW0741 , staau-MW2456 , staau-q6gfm6 , staau-SA0011 , staau-SA0569 , staau-SA0572 , staau-SA0897 , staau-SA1143 , staau-SA2240 , staau-SA2306 , staau-SA2367 , staau-SA2422 , staau-SAV0321 , staau-SAV0446 , staau-SAV0457 , staau-SAV0655 , staau-SAV1014 , staau-SAV1765 , staau-SAV1793 , staau-SAV2188 , staau-SAV2350 , staau-SAV2484 , staau-SAV2594