Gene_locus Report for: ecolx-h4unx0 Escherichia coli type VI secretion protein tle1; T6SS effector phospholipase Tle1-EAECComment Tle1EAEC has both phospholipase A1 and phospholipase A2 (PLA2) activities Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) There are 54 a/b hydrolases in Escherichia coli view in a: Table
6_AlphaBeta_hydrolase : ecok1-a1acc0Escherichia coli O1:K1 / APEC Putative uncharacterized protein. A85-EsteraseD-FGH : ecoli-yaimEscherichia coli, Escherichia albertii, S-formylglutathione hydrolase frmB, ecoli-yeiGEscherichia coli, Escherichia fergusonii, S-formylglutathione hydrolase yeiG frmC SFGH2, b2154, JW2141. A85-Feruloyl-Esterase : ecoli-yielEscherichia coli, Escherichia fergusonii, Isoamylase protein yieL. A85-IroE-IroD-Fes-Yiel : ecoli-d7xp23Escherichia coli (MS 84-1; MS 182-1; B088; O26:H11/11368/EHEC; O8/IAI1; O111:H-11128/EHEC; EC4100B; MS 78-1) Putative uncharacterized protein, ecoli-fesEscherichia coli, Escherichia fergusonii, Escherichia albertii, enterochelin esterase (ferric enterobactin esterase), ecoli-IRODEscherichia coli, Salmonella enterica, putative ferric enterochelin esterase. irod protein, ecoli-IROE Escherichia coli, Enterobactin and Salmochelin Hydrolase IroE, Salmochelin siderophore protein, ecoli-MCMKEscherichia coli ( and O157:H7/z1631/TW14359; O44:H18/042/EAEC) putative ferric enterochelin esterase mcmk mchs1 protein, ecoli-Z1341Escherichia coli hypothetical protein z1341.ABHD11-Acetyl_transferase : ecoli-ybff Escherichia coli, Escherichia albertii, Escherichia fergusonii, putative esterase/lipase YbfF (EC 3.1.-.-).ABHD13-BEM46 : ecoli-YfhREscherichia coli, Escherichia fergusonii, putative enzyme (3.4.-) hypothetical protein YfhR peptidase Z3802. abh_upf0017 : ecoli-yhetEscherichia coli, Escherichia fergusonii, Escherichia albertii, upf0017 protein yhet hypothetical 38.5 kda protein in kifb-prkb intergenic region. abh_upf00227 : ecoli-ycfpEscherichia coli, Escherichia fergusonii, Escherichia albertii, hypothetical protein ycfp, ecoli-yqiaEscherichia coli, Escherichia fergusonii, Esterase yqia. Aclacinomycin-methylesterase_RdmC : ecoli-estXEscherichia coli sat streptothricin acetyl-transferase (on plasmid) (and strains SMS-3-5/SECEC; H489 ), ecoli-rdmCEscherichia coli macrolide 2'-phosphotransferase II, homlogue of strpu-rdmC possible C-C hydrolase. AlphaBeta_hydrolase : ecolx-d6jcw3Escherichia coli B354 Predicted protein, ecolx-q707d7Escherichia coli (and O6:K15:H31/536/UPEC) Putative uncharacterized protein. BD-FAE : ecoli-C3633Escherichia coli hypothetical protein, ecoli-C3636Escherichia coli, hypothetical protein, ecolx-d6htw1Escherichia coli B088 Predicted protein, ecos5-d2nhh3Escherichia coli, Hypothetical phage protein. BioH : ecoli-bioh Escherichia coli, Escherichia fergusonii, Escherichia albertii, Escherichia sp., biotin biosynthesis bioh bioH2.Carbon-carbon_bond_hydrolase : ecoli-mhpc Escherichia coli, mhpC gene 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase in 3-hydroxy-phenylpropionic acid degradation.Dienelactone_hydrolase : ecoli-dlhhEscherichia coli, Escherichia fergusonii, Escherichia albertii, hydrolase (dlh) putative carboxymethylenebutenolidase ysgA (EC 3.1.1.45), ecoli-yghX Escherichia coli, Escherichia fergusonii, Carboxymethylenebutenolidase yghX, orf_f136.Duf_676 : eco57-b3a913Escherichia coli (O157:H7/EC4401/EC4486/EC4076/EC4196/EC508/TW14359/EC4115; MS 182-1; B/REL606) Putative uncharacterized protein. Duf_900 : ecosm-b1lhu2Escherichia coli (strain SMS-3-5 / SECEC) Conserved domain protein. Duf_1100-R : ecoli-yafaEscherichia coli, Escherichia fergusonii, Escherichia albertii, (EC 3.1.-.-) esterase yafa Fermentation-respiration switch protein frsA. Hormone-sensitive_lipase_like : ecoli-Aes Escherichia coli, Escherichia fergusonii, acetylesterase lipase like enzyme YBAC Aes Escherichia fergusonii, ecoli-C4836Escherichia coli, Escherichia albertii, putative lipase.LYsophospholipase_carboxylesterase : ecoli-ypfhEscherichia coli, Escherichia fergusonii, Escherichia albertii, YpfH hypothetical 24.9 kda protein. Displays esterase activity toward palmitoyl-CoA and pNP-butyrate. MenH_SHCHC : ecoli-YFBB Escherichia coli, Escherichia fergusonii, Escherichia albertii, MenH (EC 2.3.1.12) 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase.Monoglyceridelipase_lysophospholip : ecoli-pldbEscherichia coli, Escherichia fergusonii, pldb lysophospholipase l2 (EC 3.1.1.5) (lecithinase b), ecoli-YNBCEscherichia coli, Escherichia fergusonii hypothetical protein ynbc. PGAP1 : ecout-q1r7l6Escherichia coli, Escherichia sp., hypothetical protein. RutD : ecoli-rutD Escherichia coli BchO gene protein rutD Pyrimidine utilization protein D ycdJ rarA.S9N_PREPL_Peptidase_S9 : ecoli-ptrbEscherichia coli, Escherichia albertii, Escherichia fergusonii, Oligopeptidase B Peptidase, S9A family, protease II. T6SS-TLE1 : ecolx-e0qx45Escherichia coli. Uncharacterized protein, ecolx-f4suw9Escherichia coli. Putative type VI secretion system effector, Hcp1 family. T6SS-TLE3 : ecoli-T6SS.TLE3 Escherichia coli Adherent-Invasive Tle3 T6SS Effector LF82_435.Thioesterase : ecoli-C2429Escherichia coli hypothetical protein HMWP1 nonribosomal peptide/polyketide synthase (EC 2.3.1.-) Yersiniabactin biosynthetic protein, Condensation domain protein, ecoli-C2451 Escherichia coli Putative thioesterase Possible Oleoyl-[acyl-carrier-protein] hydrolase, putative thioesterase, ecoli-entf Escherichia coli Thioesterase domain enterobactin synthetase component f (EC 2.7.7.-) (serine activating enzyme).Tiancimycin-TnmK-Tripeptidase-HIP : ecoli-yuarEscherichia coli hypothetical 55.7 kda protein in ompp-int intergenic region precursor, ecoli-Z1930Escherichia coli YuaR YmcE protein putative protease encoded within prophage CP-933X. Xaa-Pro-like_dom : ecoli-ypt1Escherichia coli yiea YPT1 YPT2 hypothetical 31.7 kda protein in trax-fino intergenic region encoded on plasmid. YcjY-like : ecoli-C0410Escherichia coli, Escherichia albertii, protein YcjY Dienelactone_hydrolase, ecoli-ycjy Escherichia coli hypothetical protein ycjy Dienelactone hydrolase domain protein, ecoli-Z0347Escherichia coli, hypothetical protein z0347, ecoli-Z2445Escherichia coli Dienelactone hydrolase hypothetical protein z2445.yjfP_esterase-like : ecoli-yjfpEscherichia coli, Escherichia fergusonii, hypothetical protein yjfp Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Escherichia coli 55989: N, E. Escherichia coli 042: N, E. Escherichia coli DEC6A: N, E. Escherichia coli NC101: N, E. Escherichia coli MS 145-7: N, E. Escherichia coli NA114: N, E. Escherichia coli SE15: N, E. Escherichia coli APEC O1: N, E. Escherichia coli SMS-3-5: N, E. Escherichia coli B088: N, E. Escherichia coli B354: N, E. Escherichia coli 536: N, E. Escherichia coli MS 182-1: N, E. Escherichia coli O26:H11 str. 11368: N, E. Escherichia coli IAI1: N, E. Escherichia coli O111:H- str. 11128: N, E. Escherichia coli DH1: N, E. Escherichia coli O55:H7 str. CB9615: N, E. Escherichia coli O103:H2 str. 12009: N, E. Escherichia coli IHE3034: N, E. Escherichia coli BL21: N, E. Escherichia coli B str. REL606: N, E. Escherichia coli O157:H7 str. TW14359: N, E. Escherichia coli BL21(DE3): N, E. Escherichia coli MS 185-1: N, E. Escherichia coli MS 45-1: N, E. Escherichia coli O157:H7 str. EC4501: N, E. Escherichia coli FVEC1302: N, E. Escherichia coli O157:H7 str. EC508: N, E. Escherichia coli W: N, E. Escherichia coli 53638: N, E. Escherichia coli B171: N, E. Escherichia coli O157:H7 str. EC4076: N, E. Escherichia coli O157:H7 str. EC4113: N, E. Escherichia coli MS 187-1: N, E. Escherichia coli O157:H7 str. EC869: N, E. Escherichia coli O157:H7 str. EC4486: N, E. Escherichia coli 83972: N, E. Escherichia coli MS 198-1: N, E. Escherichia coli F11: N, E. Escherichia coli O157:H7 str. EC4401: N, E. Escherichia coli KO11FL: N, E. Escherichia coli MS 196-1: N, E. Escherichia coli MS 119-7: N, E. Escherichia coli O157:H7 str. EC4196: N, E. Escherichia coli MS 116-1: N, E. Escherichia coli MS 115-1: N, E. Escherichia coli O157:H7 str. TW14588: N, E. Escherichia coli E110019: N, E. Escherichia coli MS 175-1: N, E. Escherichia coli 101-1: N, E. Escherichia coli B185: N, E. Escherichia coli UTI89: N, E. Escherichia coli E24377A: N, E. Escherichia coli O127:H6 str. E2348/69: N, E. Escherichia coli S88: N, E. Escherichia coli O157:H7 str. EC4115: N, E. Escherichia coli IAI39: N, E. Escherichia coli ED1a: N, E. Escherichia coli BW2952: N, E. Escherichia coli str. K-12 substr. DH10B: N, E. Escherichia coli HS: N, E. Escherichia coli ATCC 8739: N, E. Escherichia coli UMN026: N, E. Escherichia coli SE11: N, E. Escherichia coli E22: N, E. Escherichia coli MS 200-1: N, E. Escherichia coli B7A: N, E. Escherichia coli MS 21-1: N, E. Escherichia coli FVEC1412: N, E. Escherichia coli MS 107-1: N, E. Escherichia coli MS 69-1: N, E. Escherichia coli K-12: N, E. Escherichia coli O111:H-: N, E. Escherichia coli ETEC 1392/75: N, E. Escherichia coli O26:H-: N, E. Escherichia coli Vir68: N, E. Escherichia coli EC4100B: N, E. Escherichia coli WV_060327: N, E. Escherichia coli O157:H7 str. EC1212: N, E. Escherichia coli RN587/1: N, E. Escherichia coli 1357: N, E. Escherichia coli 1180: N, E. Escherichia coli LT-68: N, E. Escherichia coli E128010: N, E. Escherichia coli EPECa14: N, E. Escherichia coli O157:H- str. 493-89: N, E. Escherichia coli O157:H- str. H 2687: N, E. Escherichia coli O55:H7 str. 3256-97: N, E. Escherichia coli O55:H7 str. USDA 5905: N, E. Escherichia coli O157:H7 str. LSU-61: N, E. Escherichia coli MS 146-1: N, E. Escherichia coli MS 78-1: N, E. Escherichia coli MS 124-1: N, E. Escherichia coli ABU 83972: N, E. Escherichia coli UM146: N, E. Escherichia coli O157:H7 str. EC4206: N, E. Escherichia coli O157:H7 str. EC4045: N, E. Escherichia coli O157:H7 str. EC4042: N, E. Escherichia coli 1827-70: N, E. Escherichia coli ETEC H10407: N, E. Escherichia coli 2362-75: N, E. Escherichia coli O83:H1 str. NRG 857C: N, E. Escherichia coli MS 110-3: N, E. Escherichia coli MS 153-1: N, E. Escherichia coli MS 16-3: N, E. Escherichia coli 3431: N, E. Escherichia coli MS 85-1: N, E. Escherichia coli O157:H7 str. G5101: N, E. Escherichia coli O157:H7: N, E. Escherichia coli O6: N, E. Escherichia coli MS 84-1: N, E. Escherichia coli 96.154: N, E. Escherichia coli DEC7B: N, E. Escherichia coli DEC3E: N, E. Escherichia coli 907446: N, E. Escherichia coli E1167: N, E. Escherichia coli BWH 34: N, E. Escherichia coli H730: N, E. Escherichia coli DEC13D: N, E. Escherichia coli HVH 78 (4-2735946): N, E. Escherichia coli UMEA 4075-1: N, E. Escherichia coli STEC_S1191: N, E. Escherichia coli 97.0246: N, E. Escherichia coli TA206: N, E. Escherichia coli HVH 177 (4-2876612): N, E. Escherichia coli str. 'clone D i2': N, E. Escherichia coli DEC3A: N, E. Escherichia coli KTE25: N, E. Escherichia coli DEC10A: N, E. Escherichia coli 95.0183: N, E. Escherichia coli O25b:H4-ST131 str. EC958: N, E. Escherichia coli 3.2303: N, E. Escherichia coli HVH 90 (4-3191362): N, E. Escherichia coli KTE106: N, E. Escherichia coli KTE173: N, E. Escherichia coli HVH 115 (4-4465989): N, E. Escherichia coli MS 60-1: N, E. Escherichia coli KTE15: N, E. Escherichia coli DEC7E: N, E. Escherichia coli STEC_EH250: N, E. Escherichia coli 907700: N, E. Escherichia coli DEC10C: N, E. Escherichia coli KTE215: N, E. Escherichia coli 3030-1: N, E. Escherichia coli DEC15B: N, E. Escherichia coli KTE162: N, E. Escherichia coli DEC3D: N, E. Escherichia coli KTE186: N, E. Escherichia coli 93.0624: N, E. Escherichia coli TW10509: N, E. Escherichia coli DEC9E: N, E. Escherichia coli 1.2264: N, E. Escherichia coli KTE43: N, E. Escherichia coli B41: N, E. Escherichia coli DEC11B: N, E. Escherichia coli UMEA 3244-1: N, E. Escherichia coli 908632: N, E. Escherichia coli DEC13C: N, E. Escherichia coli DEC5E: N, E. Escherichia coli TW07793: N, E. Escherichia coli H494: N, E. Escherichia coli DEC2A: N, E. Escherichia coli H397: N, E. Escherichia coli DEC3F: N, E. Escherichia coli HVH 138 (4-6066704): N, E. Escherichia coli IS35: N, E. Escherichia coli 9.0111: N, E. Escherichia coli DEC7C: N, E. Escherichia coli STEC_H.1.8: N, E. Escherichia coli DEC2D: N, E. Escherichia coli KTE89: N, E. Escherichia coli 99.0848: N, E. Escherichia coli HVH 202 (4-3163997): N, E. Escherichia coli 4.0522: N, E. Escherichia coli 5.0959: N, E. Escherichia coli O55:H7 str. RM12579: N, E. Escherichia coli CFT073: N, E. Escherichia coli DEC8C: N, E. Escherichia coli DEC3C: N, E. Escherichia coli KTE169: N, E. Escherichia coli CB7326: N, E. Escherichia coli O104:H4 str. 01-09591: N, E. Escherichia coli O157:H7 str. 1125: N, E. Escherichia coli SCI-07: N, E. Escherichia coli DEC10E: N, E. Escherichia coli TA007: N, E. Escherichia coli 2534-86: N, E. Escherichia coli 3.3884: N, E. Escherichia coli DEC6B: N, E. Escherichia coli O104:H4 str. 11-4522: N, E. Escherichia coli KTE16: N, E. Escherichia coli KTE195: N, E. Escherichia coli 0.1288: N, E. Escherichia coli O104:H4 str. LB226692: N, E. Escherichia coli ISC41: N, E. Escherichia coli G58-1: N, E. Escherichia coli DEC11D: N, E. Escherichia coli O157:H7 str. 1044: N, E. Escherichia coli UMEA 3108-1: N, E. Escherichia coli 900105 (10e): N, E. Escherichia coli 2.3916: N, E. Escherichia coli DEC9D: N, E. Escherichia coli AA86: N, E. Escherichia coli O104:H4 str. 11-4623: N, E. Escherichia coli DEC1A: N, E. Escherichia coli DEC14B: N, E. Escherichia coli TA271: N, E. Escherichia coli TA124: N, E. Escherichia coli DEC14C: N, E. Escherichia coli HVH 176 (4-3428664): N, E. Escherichia coli HVH 24 (4-5985145): N, E. Escherichia coli XH001: N, E. Escherichia coli HVH 141 (4-5995973): N, E. Escherichia coli HVH 4 (4-7276109): N, E. Escherichia coli KTE28: N, E. Escherichia coli DEC4C: N, E. Escherichia coli DEC8B: N, E. Escherichia coli E101: N, E. Escherichia coli KTE145: N, E. Escherichia coli DEC4B: N, E. Escherichia coli DEC8D: N, E. Escherichia coli EC1868: N, E. Escherichia coli DEC15A: N, E. Escherichia coli DEC2E: N, E. Escherichia coli KTE133: N, E. Escherichia coli IS29: N, E. Escherichia coli HVH 108 (4-6924867): N, E. Escherichia coli DEC12B: N, E. Escherichia coli TA143: N, E. Escherichia coli H120: N, E. Escherichia coli KTE207: N, E. Escherichia coli AI27: N, E. Escherichia coli Bd5610_99: N, E. Escherichia coli KTE216: N, E. Escherichia coli DEC12D: N, E. Escherichia coli DEC13A: N, E. Escherichia coli KTE93: N, E. Escherichia coli KTE102: N, E. Escherichia coli DEC2C: N, E. Escherichia coli UMNF18: N, E. Escherichia coli DEC10B: N, E. Escherichia coli HVH 21 (4-4517873): N, E. Escherichia coli UMEA 3155-1: N, E. Escherichia coli EC4437: N, E. Escherichia coli HVH 17 (4-7473087): N, E. Escherichia coli 5.0588: N, E. Escherichia coli O104:H4 str. 11-4632 C2: N, E. Escherichia coli KTE88: N, E. Escherichia coli HVH 162 (4-5627982): N, E. Escherichia coli KTE211: N, E. Escherichia coli B093: N, E. Escherichia coli 3.2608: N, E. Escherichia coli STEC_MHI813: N, E. Escherichia coli JJ1886: N, E. Escherichia coli NE1487: N, E. Escherichia coli DEC5B: N, E. Escherichia coli DEC11E: N, E. Escherichia coli KTE109: N, E. Escherichia coli B799: N, E. Escherichia coli STEC_7v: N, E. Escherichia coli O104:H4 str. 11-4632 C5: N, E. Escherichia coli DEC15D: N, E. Escherichia coli DEC9B: N, E. Escherichia coli DEC6C: N, E. Escherichia coli KTE175: N, E. Escherichia coli KTE18: N, E. Escherichia coli 1.2741: N, E. Escherichia coli KTE168: N, E. Escherichia coli DEC15C: N, E. Escherichia coli 908624: N, E. Escherichia coli BIDMC 20B: N, E. Escherichia coli STEC_DG131-3: N, E. Escherichia coli DEC8E: N, E. Escherichia coli DEC15E: N, E. Escherichia coli LAU-EC7: N, E. Escherichia coli W26: N, E. Escherichia coli DEC13E: N, E. Escherichia coli O104:H4 str. C236-11: N, E. Escherichia coli KTE183: N, E. Escherichia coli 907779: N, E. Escherichia coli TW14313: N, E. Escherichia coli E1520: N, E. Escherichia coli KTE23: N, E. Escherichia coli KTE139: N, E. Escherichia coli MS 57-2: N, E. Escherichia coli KTE49: N, E. Escherichia coli O7:K1 str. CE10: N, E. Escherichia coli J53: N, E. Escherichia coli 96.0497: N, E. Escherichia coli O104:H4 str. 11-3677: N, E. Escherichia coli H263: N, E. Escherichia coli BIDMC 38: N, E. Escherichia coli DEC14D: N, E. Escherichia coli M605: N, E. Escherichia coli KTE191: N, E. Escherichia coli BWH 24: N, E. Escherichia coli STEC_94C: N, E. Escherichia coli UMEA 3193-1: N, E. Escherichia coli KTE150: N, E. Escherichia coli DEC4A: N, E. Escherichia coli UMEA 3391-1: N, E. Escherichia coli DEC12C: N, E. Escherichia coli KTE86: N, E. Escherichia coli KTE189: N, E. Escherichia coli B49-2: N, E. Escherichia coli HVH 38 (4-2774682): N, E. Escherichia coli DEC7D: N, E. Escherichia coli HVH 205 (4-3094677): N, E. Escherichia coli M718: N, E. Escherichia coli H591: N, E. Escherichia coli 908691: N, E. Escherichia coli 2-005-03_S4_C2: N, E. Escherichia coli HVH 111 (4-7039018): N, E. Escherichia coli DEC5C: N, E. Escherichia coli KTE6: N, E. Escherichia coli BIDMC 20A: N, E. Escherichia coli JB1-95: N, E. Escherichia coli DEC9A: N, E. Escherichia coli DEC6E: N, E. Escherichia coli O104:H4 str. 11-4632 C3: N, E. Escherichia coli DEC11C: N, E. Escherichia coli O104:H4 str. 04-8351: N, E. Escherichia coli 907701: N, E. Escherichia coli cloneA_i1: N, E. Escherichia coli DEC6D: N, E. Escherichia coli O32:H37 str. P4: N, E. Escherichia coli DEC1C: N, E. Escherichia coli DEC10D: N, E. Escherichia coli DEC7A: N, E. Escherichia coli KTE192: N, E. Escherichia coli DEC12E: N, E. Escherichia coli H252: N, E. Escherichia coli DEC4F: N, E. Escherichia coli O104:H4 str. 09-7901: N, E. Escherichia coli UMEA 3693-1: N, E. Escherichia coli HVH 146 (4-3189767): N, E. Escherichia coli KTE194: N, E. Escherichia coli 2.4168: N, E. Escherichia coli H736: N, E. Escherichia coli KTE104: N, E. Escherichia coli DEC2B: N, E. Escherichia coli UMEA 3805-1: N, E. Escherichia coli HVH 154 (4-5636698): N, E. Escherichia coli DEC13B: N, E. Escherichia coli DEC3B: N, E. Escherichia coli O104:H4 str. 11-4632 C1: N, E. Escherichia coli HVH 171 (4-3191958): N, E. Escherichia coli HVH 186 (4-3405044): N, E. Escherichia coli 908524: N, E. Escherichia coli KTE70: N, E. Escherichia coli DEC5D: N, E. Escherichia coli O104:H4 str. 11-4632 C4: N, E. Escherichia coli DEC1D: N, E. Escherichia coli H489: N, E. Shigella sp. D9: N, E. Escherichia coli 3003: N, E. Escherichia coli O81:NM str. 02-3012: N, E. Escherichia coli DEC8A: N, E. Escherichia coli O104:H4 str. C227-11: N, E. Escherichia coli DEC14A: N, E. Escherichia coli KTE17: N, E. Escherichia coli TX1999: N, E. Escherichia coli 4.0967: N, E. Escherichia coli DEC11A: N, E. Escherichia coli UMNK88: N, E. Escherichia coli STEC_B2F1: N, E. Escherichia coli ECC-1470: N, E. Escherichia coli UMEA 3185-1: N, E. Escherichia coli STEC_C165-02: N, E. Escherichia coli KTE224: N, E. Escherichia coli DEC10F: N, E. Escherichia coli DEC4E: N, E. Escherichia coli E482: N, E. Escherichia coli KTE217: N, E. Escherichia coli HVH 143 (4-5674999): N, E. Escherichia coli DEC1E: N, E. Escherichia coli O104:H21 str. CFSAN002236: N, E. Escherichia coli 4_1_47FAA: N, E. Escherichia coli DEC5A: N, E. Escherichia coli UMEA 3955-1: N, E. Escherichia coli Xuzhou21: N, E. Escherichia coli HVH 228 (4-7787030): N, E. Escherichia coli str. K-12 substr. MDS42: N, E. Escherichia coli KTE72: N, E. Escherichia coli DEC12A: N, E. Escherichia coli H299: N, E. Escherichia coli KTE148: N, E. Escherichia coli CAG:4: N, E. Escherichia coli HVH 84 (4-1021478): N, E. Escherichia coli BIDMC 83: N, E. Escherichia coli PCN033: N, E. Escherichia coli HVH 197 (4-4466217): N, E. Escherichia coli DEC4D: N, E. Escherichia coli MS 117-3: N, E. Escherichia coli M863: N, E. Escherichia coli KTE75: N, E. Escherichia coli KTE113: N, E. Escherichia coli O104:H4 str. 11-4404: N, E. Escherichia coli DEC1B: N, E. Escherichia coli KTE178: N, E. Escherichia coli O104:H21 str. 94-3025: N, E. Escherichia coli 2-005-03_S4_C3: N, E. Escherichia coli DEC9C: N, E. Escherichia coli str. 'clone D i14': N, E. Escherichia coli O139:H28 str. E24377A: N, E. Escherichia coli OK1357: N, E. Escherichia coli OK1180: N, E. Escherichia coli KOEGE 61 (174a): N, E. Escherichia coli HVH 33 (4-2174936): N, E. Escherichia coli KTE98: N, E. Escherichia coli TW09098: N, E. Escherichia coli M919: N, E. Escherichia coli HVH 79 (4-2512823): N, E. Escherichia coli SEPT362: N, E. Escherichia coli O08: N, E. Escherichia coli ISC56: N, E. Escherichia coli D9: N, E. Escherichia coli ISC11: N, E. Escherichia coli 1-176-05_S3_C2: N, E. Escherichia coli O145:H28 str. RM12581: N, E. Escherichia coli O145:NM str. 2010C-3526: N, E. Escherichia coli UCI 65: N, E. Escherichia coli 5-366-08_S1_C3: N, E. Escherichia coli 5-366-08_S1_C1: N, E. Escherichia coli 1-250-04_S3_C2: N, E. Escherichia coli UMEA 3162-1: N, E. Escherichia coli O157:H7 str. SS52: N, E. Escherichia coli APEC O18: N, E. Escherichia coli N37122PS: N, E. Escherichia coli N37139PS: N, E. Escherichia coli N36410PS: N, E. Escherichia coli M056: N, E. Escherichia coli TA447: N, E. Escherichia coli H461: N, E. Escherichia coli H605: N, E. Escherichia coli TA054: N, E. Escherichia coli TA249: N, E. Escherichia coli KTE21: N, E. Escherichia coli KTE76: N, E. Escherichia coli KTE146: N, E. Escherichia coli 3.4880: N, E. Escherichia coli KTE20: N, E. Escherichia coli KTE182: N, E. Escherichia coli HVH 25 (4-5851939): N, E. Escherichia coli HVH 41 (4-2677849): N, E. Escherichia coli HVH 70 (4-2963531): N, E. Escherichia coli UMEA 3200-1: N, E. Escherichia coli UMEA 3718-1: N, E. Escherichia coli 113290: N, E. Escherichia coli 110957: N, E. Escherichia coli 907713: N, E. Escherichia coli 907672: N, E. Escherichia coli 908519: N, E. Escherichia coli 908525: N, E. Escherichia coli 908573: N, E. Escherichia coli UMEA 3323-1: N, E. Escherichia coli 97.0259: N, E. Escherichia coli LAU-EC10: N, E. Escherichia coli 1-182-04_S3_C3: N, E. Escherichia coli 3-373-03_S4_C2: N, E. Escherichia coli 2-011-08_S1_C1: N, E. Escherichia coli M114: N, E. Escherichia coli TA280: N, E. Escherichia coli KTE66: N, E. Escherichia coli DORA_A_5_14_21: N, E. Escherichia coli D6-113.11: N, E. Escherichia coli O174:H8 str. 04-3038: N, E. Escherichia coli O111:NM str. K6722: N, E. Escherichia coli O69:H11 str. 08-4661: N, E. Escherichia coli O118:H16 str. 2009C-4446: N, E. Escherichia coli O26:H11: N, E. Escherichia coli O121:H19 str. 2010C-3609: N, E. Escherichia coli N36254PS: N, E. Escherichia coli SHECO001: N, E. Escherichia coli H420: N, E. Escherichia coli O111:H8 str. CVM9634: N, E. Escherichia coli O111:H11 str. CVM9455: N, E. Escherichia coli KTE193: N, E. Escherichia coli KTE112: N, E. Escherichia coli KTE64: N, E. Escherichia coli KTE100: N, E. Escherichia coli 99.0741: N, E. Escherichia coli ATCC BAA-2209: N, E.
Sequence
References
Flaugnatti N, Rapisarda C, Rey M, Beauvois SG, Nguyen VA, Canaan S, Durand E, Chamot-Rooke J, Cascales E and Journet L <1 more author(s)> Flaugnatti N, Rapisarda C, Rey M, Beauvois SG, Nguyen VA, Canaan S, Durand E, Chamot-Rooke J, Cascales E, Fronzes R, Journet L (- 1) Ref: EMBO Journal, 39:e104129, 2020 : PubMed ESTHER: Flaugnatti_2020_EMBO.J_39_e104129 PubMedSearch: Flaugnatti 2020 EMBO.J 39 e104129 PubMedID: 32350888 Gene_locus related to this paper: ecolx-h4unx0 Abstract The bacterial type VI secretion system (T6SS) is a macromolecular machine that injects effectors into prokaryotic and eukaryotic cells. The mode of action of the T6SS is similar to contractile phages: the contraction of a sheath structure pushes a tube topped by a spike into target cells. Effectors are loaded onto the spike or confined into the tube. In enteroaggregative Escherichia coli, the Tle1 phospholipase binds the C-terminal extension of the VgrG trimeric spike. Here, we purify the VgrG-Tle1 complex and show that a VgrG trimer binds three Tle1 monomers and inhibits their activity. Using covalent cross-linking coupled to high-resolution mass spectrometry, we provide information on the sites of contact and further identify the requirement for a Tle1 N-terminal secretion sequence in complex formation. Finally, we report the 2.6-A-resolution cryo-electron microscopy tri-dimensional structure of the (VgrG)(3) -(Tle1)(3) complex revealing how the effector binds its cargo, and how VgrG inhibits Tle1 phospholipase activity. The inhibition of Tle1 phospholipase activity once bound to VgrG suggests that Tle1 dissociation from VgrG is required upon delivery. Title: A phospholipase A1 antibacterial Type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery Flaugnatti N, Le TT, Canaan S, Aschtgen MS, Nguyen VS, Blangy S, Kellenberger C, Roussel A, Cambillau C and Journet L <1 more author(s)> Flaugnatti N, Le TT, Canaan S, Aschtgen MS, Nguyen VS, Blangy S, Kellenberger C, Roussel A, Cambillau C, Cascales E, Journet L (- 1) Ref: Molecular Microbiology, 99:1099, 2016 : PubMed ESTHER: Flaugnatti_2016_Mol.Microbiol_99_1099 PubMedSearch: Flaugnatti 2016 Mol.Microbiol 99 1099 PubMedID: 26714038 Gene_locus related to this paper: ecolx-h4unx0 Abstract The Type VI secretion system (T6SS) is a multiprotein machine that delivers protein effectors in both prokaryotic and eukaryotic cells, allowing interbacterial competition and virulence. The mechanism of action of the T6SS requires the contraction of a sheath-like structure that propels a needle towards target cells, allowing the delivery of protein effectors. Here, we provide evidence that the entero-aggregative Escherichia coli Sci-1 T6SS is required to eliminate competitor bacteria. We further identify Tle1, a toxin effector encoded by this cluster and showed that Tle1 possesses phospholipase A1 and A2 activities required for the interbacterial competition. Self-protection of the attacker cell is secured by an outer membrane lipoprotein, Tli1, which binds Tle1 in a 1:1 stoichiometric ratio with nanomolar affinity, and inhibits its phospholipase activity. Tle1 is delivered into the periplasm of the prey cells using the VgrG1 needle spike protein as carrier. Further analyses demonstrate that the C-terminal extension domain of VgrG1, including a transthyretin-like domain, is responsible for the interaction with Tle1 and its subsequent delivery into target cells. Based on these results, we propose an additional mechanism of transport of T6SS effectors in which cognate effectors are selected by specific motifs located at the C-terminus of VgrG proteins. Other Papers | ||||||||||||||||||||||||||||||||
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