Family: T6SS-TLE1

Block: X

Parent Family: No family


The type VI lipase effectors Tle1-Tle5 secreted by the bacterial type VI secretion system (T6SS) have recently been identified as antibacterial effectors that hydrolyze membrane phospholipids. The Tle1-4 display phospholipase A1 and A2 (PLA1) (PLA2) activities. Tle1 belongs to Duf_2235 (T6SS-TLE1) family, TLE2 to Lipase_3, TLE3 to alpha/ beta hydrolase(now the family (T6SS-TLE3) and Tle4 belongs to PGAP1. The adjacent genes code for VgrG proteins and putative periplasmic immunity proteins Tle5 are unrelated phospholipase D type. The bacterial type VI secretion system (T6SS) is a macromolecular machine that injects effectors into prokaryotic and eukaryotic cells. In enteroaggregative Escherichia coli, the Tle1 phospholipase binds the C-terminal extension of the VgrG trimeric spike. The inhibition of Tle1 phospholipase activity once bound to VgrG suggests that Tle1 dissociation from VgrG is required upon delivery


Interpro : IPR018712 Protein of unknown function DUF2235


Pdoc : No Pdoc

Pfam : PF09994 DUF2235

Prints : No Print

EC Number : No EC Number


Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Structure Name Proteins
6SJL Structure of the Tle1 effector bound to the VgrG spike from the Type 6 secretion system ecolx-h4unx0
6SKI The Tle hydrolase bound to the TTR domain of the VgrG spike of the Type 6 secretion system ecolx-h4unx0
4O5P Crystal structure of the type VI secretion phospholipase effector Tle1 protein from Pseudomonas aeruginosa pseae-q9hyv3

Gene_locus Name Species
ecolx-h4unx0 Escherichia coli type VI secretion protein tle1\; T6SS effector phospholipase Tle1-EAEC Escherichia coli
pseae-q9hyv3 Pseudomonas aeruginosa\; Pseudomonas fluorescens\; Pseudomonas sp. the type VI secretion phospholipase effector Tle1 protein Pseudomonas aeruginosa
vibcl-c2caa4 Vibrio cholerae 12129(1). Putative uncharacterized protein Vibrio cholerae 12129(1)
9gamm-g2e7i5 Thiorhodococcus drewsii AZ1. Putative uncharacterized protein Thiorhodococcus drewsii AZ1
rosdo-q169e0 Roseobacter denitrificans (strain ATCC 33942 \/ OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans). Uncharacterized protein Roseobacter denitrificans
9gamm-g7eka6 Pseudoalteromonas sp. BSi20652. Putative uncharacterized protein Pseudoalteromonas sp. BSi20652
parbp-c0s816 Paracoccidioides brasiliensis (strain Pb03). Uncharacterized protein Paracoccidioides brasiliensis
erwam-e5b8z0 Erwinia amylovora ATCC BAA-2158. Putative uncharacterized protein Erwinia amylovora ATCC BAA-2158
yeasv-e7ltm5 Saccharomyces cerevisiae (strain VIN 13) (Baker's yeast). YEL023C-like protein Saccharomyces cerevisiae
9rhob-a3jtx5 Rhodobacteraceae bacterium HTCC2150. Uncharacterized protein Rhodobacteraceae bacterium HTCC2150
burca-q1bk56 Burkholderia cenocepacia Uncharacterized protein Burkholderia cenocepacia
9gamm-h1g1g9 Ectothiorhodospira sp. PHS-1. Uncharacterized protein Ectothiorhodospira sp. PHS-1
aspnc-a2qbp6 Aspergillus niger (strain CBS 513.88 \/ FGSC A1513). Similarity to hypothetical protein PA2063 - Pseudomonas aeruginosa Aspergillus niger
penmq-b6qcc8 Penicillium marneffei (strain ATCC 18224 \/ CBS 334.59 \/ QM 7333). Putative uncharacterized protein Penicillium marneffei
triec-f2pp24 Trichophyton equinum (strain ATCC MYA-4606 \/ CBS 127.97) (Horse ringworm fungus). Putative uncharacterized protein Trichophyton equinum
9brad-i0gfv2 Bradyrhizobium sp. S23321. Uncharacterized protein Bradyrhizobium sp. S23321
ajecn-a6qtc4 Ajellomyces capsulatus (strain NAm1 \/ WU24) (Darling's disease fungus) (Histoplasma capsulatum). Predicted protein Ajellomyces capsulatus
varpe-e6uvk9 Variovorax paradoxus (strain EPS). Uncharacterized protein Variovorax paradoxus
metsb-b8ekf2 Methylocella silvestris (strain BL2 \/ DSM 15510 \/ NCIMB 13906). Uncharacterized protein Methylocella silvestris
psepg-b0kg49 Pseudomonas putida (strain GB-1). Rhs element Vgr protein Pseudomonas putida
metar-e9f0w8 Metarhizium anisopliae (strain ARSEF 23 \/ ATCC MYA-3075). Putative uncharacterized protein Metarhizium anisopliae
pyrtt-e3s3p6 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres). Putative uncharacterized protein Pyrenophora teres f. teres
pospm-b8pa20.1 Postia placenta (strain ATCC 44394 \/ Madison 698-R) (Brown rot fungus) (Poria monticola). Predicted protein Postia placenta
pospm-b8pa20.2 Postia placenta (strain ATCC 44394 \/ Madison 698-R) (Brown rot fungus) (Poria monticola). Predicted protein Postia placenta
9alte-a3jfp8 Marinobacter sp. ELB17. Uncharacterized protein Marinobacter sp. ELB17
metea-c5as87 Methylobacterium extorquens (strain ATCC 14718 \/ DSM 1338 \/ AM1). Uncharacterized protein Methylobacterium extorquens
pirid-g4u2y4 Piriformospora indica (strain DSM 11827). Related to protein involved in cell growth Piriformospora indica
ecolx-e0qx45 Escherichia coli. Uncharacterized protein Escherichia coli
klepn-a0a016qwx4 Klebsiella pneumoniae Uncharacterized protein Klebsiella pneumoniae Kb677
pseae-q9i252 Pseudomonas aeruginosa, Pseudomonas sp. PA2063 Uncharacterized protein Pseudomonas aeruginosa
ecolx-f4suw9 Escherichia coli. Putative type VI secretion system effector, Hcp1 family Escherichia coli
ecolx-e1iyu3 Escherichia coli. Uncharacterized protein Escherichia coli MS 145-7
ecolx-g0dan0 Escherichia coli. Uncharacterized protein Escherichia coli NA114

Gene_locus_frgt Name Species
cyth3-q11xb7 Cytophaga hutchinsonii (strain ATCC 33406 \/ NCIMB 9469). Uncharacterized protein Cytophaga hutchinsonii

Substrate Chemical Nomenclature Proteins
Tween-20 2-[2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy]ethyl dodecanoate ustma-q4pep1    human-LYPLA1

References (4)

Title : The phospholipase effector Tle1(Vc) promotes Vibrio cholerae virulence by killing competitors and impacting gene expression - Liu_2023_Gut.Microbes_15_2241204
Author(s) : Liu M , Wang H , Liu Y , Tian M , Wang Z , Shu RD , Zhao MY , Chen WD , Fu Y
Ref : Gut Microbes , 15 :2241204 , 2023
Abstract :
PubMedSearch : Liu_2023_Gut.Microbes_15_2241204
PubMedID: 37526354

Title : Structural basis for loading and inhibition of a bacterial T6SS phospholipase effector by the VgrG spike - Flaugnatti_2020_EMBO.J_39_e104129
Author(s) : Flaugnatti N , Rapisarda C , Rey M , Beauvois SG , Nguyen VA , Canaan S , Durand E , Chamot-Rooke J , Cascales E , Fronzes R , Journet L
Ref : EMBO Journal , 39 :e104129 , 2020
Abstract :
PubMedSearch : Flaugnatti_2020_EMBO.J_39_e104129
PubMedID: 32350888
Gene_locus related to this paper: ecolx-h4unx0

Title : Structure of the type VI secretion phospholipase effector Tle1 provides insight into its hydrolysis and membrane targeting - Hu_2014_Acta.Crystallogr.D.Biol.Crystallogr_70_2175
Author(s) : Hu H , Zhang H , Gao Z , Wang D , Liu G , Xu J , Lan K , Dong Y
Ref : Acta Crystallographica D Biol Crystallogr , 70 :2175 , 2014
Abstract :
PubMedSearch : Hu_2014_Acta.Crystallogr.D.Biol.Crystallogr_70_2175
PubMedID: 25084336
Gene_locus related to this paper: pseae-q9hyv3

Title : Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors - Russell_2013_Nature_496_508
Author(s) : Russell AB , LeRoux M , Hathazi K , Agnello DM , Ishikawa T , Wiggins PA , Wai SN , Mougous JD
Ref : Nature , 496 :508 , 2013
Abstract :
PubMedSearch : Russell_2013_Nature_496_508
PubMedID: 23552891
Gene_locus related to this paper: ecoli-T6SS.TLE3