T6SS-TLE1
Comment
The type VI lipase effectors Tle1-Tle5 secreted by the bacterial type VI secretion system (T6SS) have recently been identified as antibacterial effectors that hydrolyze membrane phospholipids. The Tle1-4 display phospholipase A1 and A2 (PLA1) (PLA2) activities. Tle1 belongs to Duf_2235 (T6SS-TLE1) family, TLE2 to Lipase_3, TLE3 to alpha/ beta hydrolase(now the family (T6SS-TLE3) and Tle4 belongs to PGAP1. The adjacent genes code for VgrG proteins and putative periplasmic immunity proteins Tle5 are unrelated phospholipase D type. The bacterial type VI secretion system (T6SS) is a macromolecular machine that injects effectors into prokaryotic and eukaryotic cells. In enteroaggregative Escherichia coli, the Tle1 phospholipase binds the C-terminal extension of the VgrG trimeric spike. The inhibition of Tle1 phospholipase activity once bound to VgrG suggests that Tle1 dissociation from VgrG is required upon delivery
Database
Interpro : IPR018712 Protein of unknown function DUF2235
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF09994 DUF2235
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 4O5P | Crystal structure of the type VI secretion phospholipase effector Tle1 protein from Pseudomonas aeruginosa | pseae-q9hyv3 |
| 6SJL | Structure of the Tle1 effector bound to the VgrG spike from the Type 6 secretion system | ecolx-h4unx0 |
| 6SKI | The Tle hydrolase bound to the TTR domain of the VgrG spike of the Type 6 secretion system | ecolx-h4unx0 |
| Gene_locus | Name | Species |
|---|---|---|
| 9alte-a3jfp8 | Marinobacter sp. ELB17. Uncharacterized protein | Marinobacter sp. ELB17 |
| 9brad-i0gfv2 | Bradyrhizobium sp. S23321. Uncharacterized protein | Bradyrhizobium sp. S23321 |
| 9gamm-g2e7i5 | Thiorhodococcus drewsii AZ1. Putative uncharacterized protein | Thiorhodococcus drewsii AZ1 |
| 9gamm-g7eka6 | Pseudoalteromonas sp. BSi20652. Putative uncharacterized protein | Pseudoalteromonas sp. BSi20652 |
| 9gamm-h1g1g9 | Ectothiorhodospira sp. PHS-1. Uncharacterized protein | Ectothiorhodospira sp. PHS-1 |
| 9rhob-a3jtx5 | Rhodobacteraceae bacterium HTCC2150. Uncharacterized protein | Rhodobacteraceae bacterium HTCC2150 |
| ajecn-a6qtc4 | Ajellomyces capsulatus (strain NAm1 \/ WU24) (Darling's disease fungus) (Histoplasma capsulatum). Predicted protein | Ajellomyces capsulatus |
| aspnc-a2qbp6 | Aspergillus niger (strain CBS 513.88 \/ FGSC A1513). Similarity to hypothetical protein PA2063 - Pseudomonas aeruginosa | Aspergillus niger |
| burca-q1bk56 | Burkholderia cenocepacia Uncharacterized protein | Burkholderia cenocepacia |
| ecolx-e0qx45 | Escherichia coli. Uncharacterized protein | Escherichia coli |
| ecolx-e1iyu3 | Escherichia coli. Uncharacterized protein | Escherichia coli MS 145-7 |
| ecolx-f4suw9 | Escherichia coli. Putative type VI secretion system effector, Hcp1 family | Escherichia coli |
| ecolx-g0dan0 | Escherichia coli. Uncharacterized protein | Escherichia coli NA114 |
| ecolx-h4unx0 |  Escherichia coli type VI secretion protein tle1\; T6SS effector phospholipase Tle1-EAEC |
Escherichia coli |
| erwam-e5b8z0 | Erwinia amylovora ATCC BAA-2158. Putative uncharacterized protein | Erwinia amylovora ATCC BAA-2158 |
| klepn-a0a016qwx4 | Klebsiella pneumoniae Uncharacterized protein | Klebsiella pneumoniae Kb677 |
| metar-e9f0w8 | Metarhizium anisopliae (strain ARSEF 23 \/ ATCC MYA-3075). Putative uncharacterized protein | Metarhizium anisopliae |
| metea-c5as87 | Methylobacterium extorquens (strain ATCC 14718 \/ DSM 1338 \/ AM1). Uncharacterized protein | Methylobacterium extorquens |
| metsb-b8ekf2 | Methylocella silvestris (strain BL2 \/ DSM 15510 \/ NCIMB 13906). Uncharacterized protein | Methylocella silvestris |
| parbp-c0s816 | Paracoccidioides brasiliensis (strain Pb03). Uncharacterized protein | Paracoccidioides brasiliensis |
| penmq-b6qcc8 | Penicillium marneffei (strain ATCC 18224 \/ CBS 334.59 \/ QM 7333). Putative uncharacterized protein | Penicillium marneffei |
| pirid-g4u2y4 | Piriformospora indica (strain DSM 11827). Related to protein involved in cell growth | Piriformospora indica |
| pospm-b8pa20.1 | Postia placenta (strain ATCC 44394 \/ Madison 698-R) (Brown rot fungus) (Poria monticola). Predicted protein | Postia placenta |
| pospm-b8pa20.2 | Postia placenta (strain ATCC 44394 \/ Madison 698-R) (Brown rot fungus) (Poria monticola). Predicted protein | Postia placenta |
| pseae-q9hyv3 | Pseudomonas aeruginosa\; Pseudomonas fluorescens\; Pseudomonas sp. the type VI secretion phospholipase effector Tle1 protein |
Pseudomonas aeruginosa |
| pseae-q9i252 | Pseudomonas aeruginosa, Pseudomonas sp. PA2063 Uncharacterized protein | Pseudomonas aeruginosa |
| psepg-b0kg49 | Pseudomonas putida (strain GB-1). Rhs element Vgr protein | Pseudomonas putida |
| pyrtt-e3s3p6 | Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres). Putative uncharacterized protein | Pyrenophora teres f. teres |
| rosdo-q169e0 | Roseobacter denitrificans (strain ATCC 33942 \/ OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans). Uncharacterized protein | Roseobacter denitrificans |
| triec-f2pp24 | Trichophyton equinum (strain ATCC MYA-4606 \/ CBS 127.97) (Horse ringworm fungus). Putative uncharacterized protein | Trichophyton equinum |
| varpe-e6uvk9 | Variovorax paradoxus (strain EPS). Uncharacterized protein | Variovorax paradoxus |
| vibcl-c2caa4 | Vibrio cholerae 12129(1). Putative uncharacterized protein | Vibrio cholerae 12129(1) |
| yeasv-e7ltm5 | Saccharomyces cerevisiae (strain VIN 13) (Baker's yeast). YEL023C-like protein | Saccharomyces cerevisiae |
| Gene_locus_frgt | Name | Species |
|---|---|---|
| cyth3-q11xb7 | Cytophaga hutchinsonii (strain ATCC 33406 \/ NCIMB 9469). Uncharacterized protein | Cytophaga hutchinsonii |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| Tween-20 | 2-[2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy]ethyl dodecanoate | ustma-q4pep1 human-LYPLA1 |
References (4)
| Title : The phospholipase effector Tle1(Vc) promotes Vibrio cholerae virulence by killing competitors and impacting gene expression - Liu_2023_Gut.Microbes_15_2241204 |
| Author(s) : Liu M , Wang H , Liu Y , Tian M , Wang Z , Shu RD , Zhao MY , Chen WD , Fu Y |
| Ref : Gut Microbes , 15 :2241204 , 2023 |
| Abstract : |
| PubMedSearch : Liu_2023_Gut.Microbes_15_2241204 |
| PubMedID: 37526354 |
| Title : Structural basis for loading and inhibition of a bacterial T6SS phospholipase effector by the VgrG spike - Flaugnatti_2020_EMBO.J_39_e104129 |
| Author(s) : Flaugnatti N , Rapisarda C , Rey M , Beauvois SG , Nguyen VA , Canaan S , Durand E , Chamot-Rooke J , Cascales E , Fronzes R , Journet L |
| Ref : EMBO Journal , 39 :e104129 , 2020 |
| Abstract : |
| PubMedSearch : Flaugnatti_2020_EMBO.J_39_e104129 |
| PubMedID: 32350888 |
| Gene_locus related to this paper: ecolx-h4unx0 |
| Title : Structure of the type VI secretion phospholipase effector Tle1 provides insight into its hydrolysis and membrane targeting - Hu_2014_Acta.Crystallogr.D.Biol.Crystallogr_70_2175 |
| Author(s) : Hu H , Zhang H , Gao Z , Wang D , Liu G , Xu J , Lan K , Dong Y |
| Ref : Acta Crystallographica D Biol Crystallogr , 70 :2175 , 2014 |
| Abstract : |
| PubMedSearch : Hu_2014_Acta.Crystallogr.D.Biol.Crystallogr_70_2175 |
| PubMedID: 25084336 |
| Gene_locus related to this paper: pseae-q9hyv3 |
| Title : Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors - Russell_2013_Nature_496_508 |
| Author(s) : Russell AB , LeRoux M , Hathazi K , Agnello DM , Ishikawa T , Wiggins PA , Wai SN , Mougous JD |
| Ref : Nature , 496 :508 , 2013 |
| Abstract : |
| PubMedSearch : Russell_2013_Nature_496_508 |
| PubMedID: 23552891 |
| Gene_locus related to this paper: ecoli-T6SS.TLE3 |