F297V_human-ACHE

General

Gene Locus : human-ACHE

Mode of mutation : Site directed mutagenesis

Disease :

Summary : Acyl specificity\; Acyl pocket, increases Km for ATC and increase kcat\;Ordentlich_1993_J.Biol.Chem_268_17083\;Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097 || Acyl specificity\;replacement of aromatic active center residues in human-ACHE by the corresponding residues in human-BCHE\;Kaplan_2001_Biochemistry_40_7433

AAA Change :

Allelic Variant :

Risk Factor :

Inhibitor :

Structure :

Disease by interaction :

Interact Gene Locus :

Xenobiotic sensitivity :

Modification : Acyl specificity

Torpedo_number : 290

Kinetic Parameter : Acetylthiocholine_F297V_human-ACHE, Butyrylthiocholine_F297V_human-ACHE

News : No news

Comment : "p.F297V Phe297Val (p.F328V Phe328Val in primary sequence with 31 amino-acids signal peptide) Acyl pocket, increases Km for ATC and increase kcat\; replacement of aromatic active center residues in human-ACHE by the corresponding residues in human-BCHE"

References (3)

Title : Does butyrylization of acetylcholinesterase through substitution of the six divergent aromatic amino acids in the active center gorge generate an enzyme mimic of butyrylcholinesterase? - Kaplan_2001_Biochemistry_40_7433
Author(s) : Kaplan D , Ordentlich A , Barak D , Ariel N , Kronman C , Velan B , Shafferman A
Ref : Biochemistry , 40 :7433 , 2001
Abstract : Kaplan_2001_Biochemistry_40_7433
ESTHER : Kaplan_2001_Biochemistry_40_7433
PubMedSearch : Kaplan_2001_Biochemistry_40_7433
PubMedID: 11412096

Title : Recombinant human acetylcholinesterase - Enzyme engineering -
Author(s) : Shafferman A , Velan B , Barak D , Kronman C , Ordentlich A , Flashner Y , Leitner M , Segal Y , Grosfeld H , Stein D , Ariel N
Ref : Medical Defense Bioscience Review , 3 :1097 , 1993
PubMedID:

Title : Dissection of the human acetylcholinesterase active center determinants of substrate specificity. Identification of residues constituting the anionic site, the hydrophobic site, and the acyl pocket - Ordentlich_1993_J.Biol.Chem_268_17083
Author(s) : Ordentlich A , Barak D , Kronman C , Flashner Y , Leitner M , Segall Y , Ariel N , Cohen S , Velan B , Shafferman A
Ref : Journal of Biological Chemistry , 268 :17083 , 1993
Abstract : Ordentlich_1993_J.Biol.Chem_268_17083
ESTHER : Ordentlich_1993_J.Biol.Chem_268_17083
PubMedSearch : Ordentlich_1993_J.Biol.Chem_268_17083
PubMedID: 8349597
Gene_locus related to this paper: human-ACHE , human-BCHE