Gene Locus : human-ACHE
Mode of mutation : Site directed mutagenesis
Disease :
Summary : PROSS algorithm Expression in E Coli Goldenzweig_2016_Mol.Cell_63_337
AAA Change :
Allelic Variant :
Risk Factor :
Inhibitor :
Structure : 5HQ3
Disease by interaction :
Interact Gene Locus :
Xenobiotic sensitivity :
Modification : Expression in E Coli || PROSS algorithm
Torpedo_number : 411
Kinetic Parameter : No kinetic parameter
News : No news
Comment :
p.L418Y Leu418Tyr (p.L449Y Leu449Tyr in primary sequence with 31 amino-acids signal peptide) mutation designed by -Protein Repair One Stop Shop- process for the production of active human acetylcholinesterase in a prokaryotic system. The mutation gives Tyr as in Torpedo californica and Bungar ACHE
| Title : Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability - Goldenzweig_2016_Mol.Cell_63_337 |
| Author(s) : Goldenzweig A , Goldsmith M , Hill SE , Gertman O , Laurino P , Ashani Y , Dym O , Unger T , Albeck S , Prilusky J , Lieberman RL , Aharoni A , Silman I , Sussman JL , Tawfik DS , Fleishman SJ |
| Ref : Mol Cell , 63 :337 , 2016 |
| Abstract : |
| PubMedSearch : Goldenzweig_2016_Mol.Cell_63_337 |
| PubMedID: 27425410 |
| Gene_locus related to this paper: human-ACHE |