Tawfik DS

General

Full name : Tawfik Dan S

First name : Dan S

Mail : Department of Biological Chemistry Weizmann Institute of Science, Rehovot 76100

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Country : Israel

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Website : \/\/en.wikipedia.org\/wiki\/Dan_Tawfik

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References (51)

Title : Utilization of diverse organophosphorus pollutants by marine bacteria - Despotovic_2022_Proc.Natl.Acad.Sci.U.S.A_119_e2203604119
Author(s) : Despotovic D , Aharon E , Trofimyuk O , Dubovetskyi A , Cherukuri KP , Ashani Y , Eliason O , Sperfeld M , Leader H , Castelli A , Fumagalli L , Savidor A , Levin Y , Longo LM , Segev E , Tawfik DS
Ref : Proc Natl Acad Sci U S A , 119 :e2203604119 , 2022
PubMedID: 35917352

Title : Widespread Utilization of Diverse Organophosphate Pollutants by Marine Bacteria - Despotovic_2021_Biorxiv__
Author(s) : Despotovic D , Aharon E , Trofimyuk O , Dubovetskyi A , Cherukuri KP , Ashani Y , Leader H , Castelli A , Fumagalli A , Savidor A , Levin Y , Longo LM , Segev E , Tawfik DS
Ref : Biorxiv , : , 2020
PubMedID:

Title : Enzyme evolution in natural products biosynthesis: target- or diversity-oriented? - Noda-Garcia_2020_Curr.Opin.Chem.Biol_59_147
Author(s) : Noda-Garcia L , Tawfik DS
Ref : Curr Opin Chemical Biology , 59 :147 , 2020
PubMedID: 32771972

Title : A mixture of three engineered phosphotriesterases enables rapid detoxification of the entire spectrum of known threat nerve agents - Despotovic_2019_Protein.Eng.Des.Sel_32_169
Author(s) : Despotovic D , Aharon E , Dubovetskyi A , Leader H , Ashani Y , Tawfik DS
Ref : Protein Engineering Des Sel , 32 :169 , 2019
PubMedID: 31612205

Title : Single treatment of VX poisoned guinea pigs with the phosphotriesterase mutant C23AL: Intraosseous versus intravenous injection - Wille_2016_Toxicol.Lett_258_198
Author(s) : Wille T , Neumaier K , Koller M , Ehinger C , Aggarwal N , Ashani Y , Goldsmith M , Sussman JL , Tawfik DS , Thiermann H , Worek F
Ref : Toxicol Lett , 258 :198 , 2016
PubMedID: 27397758

Title : Catalytic efficiencies of directly evolved phosphotriesterase variants with structurally different organophosphorus compounds in vitro - Goldsmith_2016_Arch.Toxicol_90_2711
Author(s) : Goldsmith M , Eckstein S , Ashani Y , Greisen P, Jr. , Leader H , Sussman JL , Aggarwal N , Ovchinnikov S , Tawfik DS , Baker D , Thiermann H , Worek F
Ref : Archives of Toxicology , 90 :2711 , 2016
PubMedID: 26612364

Title : Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability - Goldenzweig_2016_Mol.Cell_63_337
Author(s) : Goldenzweig A , Goldsmith M , Hill SE , Gertman O , Laurino P , Ashani Y , Dym O , Unger T , Albeck S , Prilusky J , Lieberman RL , Aharoni A , Silman I , Sussman JL , Tawfik DS , Fleishman SJ
Ref : Mol Cell , 63 :337 , 2016
PubMedID: 27425410
Gene_locus related to this paper: human-ACHE

Title : A new post-intoxication treatment of paraoxon and parathion poisonings using an evolved PON1 variant and recombinant GOT1 - Goldsmith_2016_Chem.Biol.Interact_259_242
Author(s) : Goldsmith M , Ashani Y , Margalit R , Nyska A , Mirelman D , Tawfik DS
Ref : Chemico-Biological Interactions , 259 :242 , 2016
PubMedID: 27256520

Title : Catalytic stimulation by restrained active-site floppiness--the case of high density lipoprotein-bound serum paraoxonase-1 - Ben-David_2015_J.Mol.Biol_427_1359
Author(s) : Ben-David M , Sussman JL , Maxwell CI , Szeler K , Kamerlin SC , Tawfik DS
Ref : Journal of Molecular Biology , 427 :1359 , 2015
PubMedID: 25644661

Title : Efficacy of the rePON1 mutant IIG1 to prevent cyclosarin toxicity in vivo and to detoxify structurally different nerve agents in vitro - Worek_2014_Arch.Toxicol_88_1257
Author(s) : Worek F , Seeger T , Goldsmith M , Ashani Y , Leader H , Sussman JL , Tawfik DS , Thiermann H , Wille T
Ref : Archives of Toxicology , 88 :1257 , 2014
PubMedID: 24477626

Title : Post-exposure treatment of VX poisoned guinea pigs with the engineered phosphotriesterase mutant C23: A proof-of-concept study - Worek_2014_Toxicol.Lett_231_45
Author(s) : Worek F , Seeger T , Reiter G , Goldsmith M , Ashani Y , Leader H , Sussman JL , Aggarwal N , Thiermann H , Tawfik DS
Ref : Toxicol Lett , 231 :45 , 2014
PubMedID: 25195526

Title : Catalytic metal ion rearrangements underline promiscuity and evolvability of a metalloenzyme - Ben-David_2013_J.Mol.Biol_425_1028
Author(s) : Ben-David M , Wieczorek G , Elias M , Silman I , Sussman JL , Tawfik DS
Ref : Journal of Molecular Biology , 425 :1028 , 2013
PubMedID: 23318950

Title : Engineering v-type nerve agents detoxifying enzymes using computationally focused libraries - Cherny_2013_ACS.Chem.Biol_8_2394
Author(s) : Cherny I , Greisen P, Jr. , Ashani Y , Khare SD , Oberdorfer G , Leader H , Baker D , Tawfik DS
Ref : ACS Chemical Biology , 8 :2394 , 2013
PubMedID: 24041203

Title : Enzyme engineering by targeted libraries - Goldsmith_2013_Methods.Enzymol_523_257
Author(s) : Goldsmith M , Tawfik DS
Ref : Methods Enzymol , 523 :257 , 2013
PubMedID: 23422434

Title : Catalytic versatility and backups in enzyme active sites: the case of serum paraoxonase 1 - Ben-David_2012_J.Mol.Biol_418_181
Author(s) : Ben-David M , Elias M , Filippi JJ , Dunach E , Silman I , Sussman JL , Tawfik DS
Ref : Journal of Molecular Biology , 418 :181 , 2012
PubMedID: 22387469

Title : Divergence and convergence in enzyme evolution: parallel evolution of paraoxonases from quorum-quenching lactonases - Elias_2012_J.Biol.Chem_287_11
Author(s) : Elias M , Tawfik DS
Ref : Journal of Biological Chemistry , 287 :11 , 2012
PubMedID: 22069329

Title : Reconstructing a missing link in the evolution of a recently diverged phosphotriesterase by active-site loop remodeling - Afriat-Jurnou_2012_Biochemistry_51_6047
Author(s) : Afriat-Jurnou L , Jackson CJ , Tawfik DS
Ref : Biochemistry , 51 :6047 , 2012
PubMedID: 22809311

Title : Evolved stereoselective hydrolases for broad-spectrum G-type nerve agent detoxification - Goldsmith_2012_Chem.Biol_19_456
Author(s) : Goldsmith M , Ashani Y , Simo Y , Ben-David M , Leader H , Silman I , Sussman JL , Tawfik DS
Ref : Chemical Biology , 19 :456 , 2012
PubMedID: 22520752

Title : Directed enzyme evolution: beyond the low-hanging fruit - Goldsmith_2012_Curr.Opin.Struct.Biol_22_406
Author(s) : Goldsmith M , Tawfik DS
Ref : Current Opinion in Structural Biology , 22 :406 , 2012
PubMedID: 22579412

Title : Computational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysis - Khare_2012_Nat.Chem.Biol_8_294
Author(s) : Khare SD , Kipnis Y , Greisen P, Jr. , Takeuchi R , Ashani Y , Goldsmith M , Song Y , Gallaher JL , Silman I , Leader H , Sussman JL , Stoddard BL , Tawfik DS , Baker D
Ref : Nat Chemical Biology , 8 :294 , 2012
PubMedID: 22306579

Title : The moderately efficient enzyme: evolutionary and physicochemical trends shaping enzyme parameters - Bar-Even_2011_Biochemistry_50_4402
Author(s) : Bar-Even A , Noor E , Savir Y , Liebermeister W , Davidi D , Tawfik DS , Milo R
Ref : Biochemistry , 50 :4402 , 2011
PubMedID: 21506553

Title : Directed evolution of hydrolases for prevention of G-type nerve agent intoxication - Gupta_2011_Nat.Chem.Biol_7_120
Author(s) : Gupta RD , Goldsmith M , Ashani Y , Simo Y , Mullokandov G , Bar H , Ben-David M , Leader H , Margalit R , Silman I , Sussman JL , Tawfik DS
Ref : Nat Chemical Biology , 7 :120 , 2011
PubMedID: 21217689

Title : Paraoxonase-1 and clopidogrel efficacy -
Author(s) : Camps J , Joven J , Mackness B , Mackness MI , Tawfik DS , Draganov DI , Costa LG , Paragh G , Seres I , Horke S , James RW , Hernandez AF , Reddy ST , Shih DM , Navab M , Rochu D , Aviram M
Ref : Nat Med , 17 :1041 , 2011
PubMedID: 21900915

Title : Directed evolution of sulfotransferases and paraoxonases by ancestral libraries - Alcolombri_2011_J.Mol.Biol_411_837
Author(s) : Alcolombri U , Elias M , Tawfik DS
Ref : Journal of Molecular Biology , 411 :837 , 2011
PubMedID: 21723874

Title : In vitro detoxification of cyclosarin in human blood pre-incubated ex vivo with recombinant serum paraoxonases - Ashani_2011_Toxicol.Lett_206_24
Author(s) : Ashani Y , Goldsmith M , Leader H , Silman I , Sussman JL , Tawfik DS
Ref : Toxicol Lett , 206 :24 , 2011
PubMedID: 21807078

Title : Stereo-specific synthesis of analogs of nerve agents and their utilization for selection and characterization of paraoxonase (PON1) catalytic scavengers - Ashani_2010_Chem.Biol.Interact_187_362
Author(s) : Ashani Y , Gupta RD , Goldsmith M , Silman I , Sussman JL , Tawfik DS , Leader H
Ref : Chemico-Biological Interactions , 187 :362 , 2010
PubMedID: 20303930

Title : ApoE induces serum paraoxonase PON1 activity and stability similar to ApoA-I - Gaidukov_2010_Biochemistry_49_532
Author(s) : Gaidukov L , Viji RI , Yacobson S , Rosenblat M , Aviram M , Tawfik DS
Ref : Biochemistry , 49 :532 , 2010
PubMedID: 20025294

Title : Directed evolution of serum paraoxonase PON3 by family shuffling and ancestor\/consensus mutagenesis, and its biochemical characterization - Khersonsky_2009_Biochemistry_48_6644
Author(s) : Khersonsky O , Rosenblat M , Toker L , Yacobson S , Hugenmatter A , Silman I , Sussman JL , Aviram M , Tawfik DS
Ref : Biochemistry , 48 :6644 , 2009
PubMedID: 19492856

Title : Stability effects of mutations and protein evolvability - Tokuriki_2009_Curr.Opin.Struct.Biol_19_596
Author(s) : Tokuriki N , Tawfik DS
Ref : Current Opinion in Structural Biology , 19 :596 , 2009
PubMedID: 19765975

Title : In vivo administration of BL-3050: highly stable engineered PON1-HDL complexes - Gaidukov_2009_BMC.Clin.Pharmacol_9_18
Author(s) : Gaidukov L , Bar D , Yacobson S , Naftali E , Kaufman O , Tabakman R , Tawfik DS , Levy-Nissenbaum E
Ref : BMC Clin Pharmacol , 9 :18 , 2009
PubMedID: 19922610

Title : Potential role of phenotypic mutations in the evolution of protein expression and stability - Goldsmith_2009_Proc.Natl.Acad.Sci.U.S.A_106_6197
Author(s) : Goldsmith M , Tawfik DS
Ref : Proc Natl Acad Sci U S A , 106 :6197 , 2009
PubMedID: 19339491

Title : How protein stability and new functions trade off - Tokuriki_2008_PLoS.Comput.Biol_4_e1000002
Author(s) : Tokuriki N , Stricher F , Serrano L , Tawfik DS
Ref : PLoS Comput Biol , 4 :e1000002 , 2008
PubMedID: 18463696

Title : Directed enzyme evolution via small and effective neutral drift libraries - Gupta_2008_Nat.Methods_5_939
Author(s) : Gupta RD , Tawfik DS
Ref : Nat Methods , 5 :939 , 2008
PubMedID: 18931667

Title : The development of human sera tests for HDL-bound serum PON1 and its lipolactonase activity - Gaidukov_2007_J.Lipid.Res_48_1637
Author(s) : Gaidukov L , Tawfik DS
Ref : J Lipid Res , 48 :1637 , 2007
PubMedID: 17435182

Title : Directed evolution by in vitro compartmentalization -
Author(s) : Miller OJ , Bernath K , Agresti JJ , Amitai G , Kelly BT , Mastrobattista E , Taly V , Magdassi S , Tawfik DS , Griffiths AD
Ref : Nat Methods , 3 :561 , 2006
PubMedID: 16791215

Title : The latent promiscuity of newly identified microbial lactonases is linked to a recently diverged phosphotriesterase - Afriat_2006_Biochemistry_45_13677
Author(s) : Afriat L , Roodveldt C , Manco G , Tawfik DS
Ref : Biochemistry , 45 :13677 , 2006
PubMedID: 17105187

Title : Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase - Amitai_2006_FEBS.J_273_1906
Author(s) : Amitai G , Gaidukov L , Adani R , Yishay S , Yacov G , Kushnir M , Teitlboim S , Lindenbaum M , Bel P , Khersonsky O , Tawfik DS , Meshulam H
Ref : Febs J , 273 :1906 , 2006
PubMedID: 16640555

Title : The catalytic histidine dyad of high density lipoprotein-associated serum paraoxonase-1 (PON1) is essential for PON1-mediated inhibition of low density lipoprotein oxidation and stimulation of macrophage cholesterol efflux - Rosenblat_2006_J.Biol.Chem_281_7657
Author(s) : Rosenblat M , Gaidukov L , Khersonsky O , Vaya J , Oren R , Tawfik DS , Aviram M
Ref : Journal of Biological Chemistry , 281 :7657 , 2006
PubMedID: 16407304

Title : The 192R\/Q polymorphs of serum paraoxonase PON1 differ in HDL binding, lipolactonase stimulation, and cholesterol efflux - Gaidukov_2006_J.Lipid.Res_47_2492
Author(s) : Gaidukov L , Rosenblat M , Aviram M , Tawfik DS
Ref : J Lipid Res , 47 :2492 , 2006
PubMedID: 16914770

Title : High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments - Aharoni_2005_Chem.Biol_12_1281
Author(s) : Aharoni A , Amitai G , Bernath K , Magdassi S , Tawfik DS
Ref : Chemical Biology , 12 :1281 , 2005
PubMedID: 16356845

Title : The 'evolvability' of promiscuous protein functions - Aharoni_2005_Nat.Genet_37_73
Author(s) : Aharoni A , Gaidukov L , Khersonsky O , Mc QGS , Roodveldt C , Tawfik DS
Ref : Nat Genet , 37 :73 , 2005
PubMedID: 15568024

Title : High-throughput screens and selections of enzyme-encoding genes - Aharoni_2005_Curr.Opin.Chem.Biol_9_210
Author(s) : Aharoni A , Griffiths AD , Tawfik DS
Ref : Curr Opin Chemical Biology , 9 :210 , 2005
PubMedID: 15811807

Title : Directed evolution of proteins for heterologous expression and stability - Roodveldt_2005_Curr.Opin.Struct.Biol_15_50
Author(s) : Roodveldt C , Aharoni A , Tawfik DS
Ref : Current Opinion in Structural Biology , 15 :50 , 2005
PubMedID: 15718133

Title : Structure-reactivity studies of serum paraoxonase PON1 suggest that its native activity is lactonase - Khersonsky_2005_Biochemistry_44_6371
Author(s) : Khersonsky O , Tawfik DS
Ref : Biochemistry , 44 :6371 , 2005
PubMedID: 15835926

Title : High affinity, stability, and lactonase activity of serum paraoxonase PON1 anchored on HDL with ApoA-I - Gaidukov_2005_Biochemistry_44_11843
Author(s) : Gaidukov L , Tawfik DS
Ref : Biochemistry , 44 :11843 , 2005
PubMedID: 16128586

Title : Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes - Harel_2004_Nat.Struct.Mol.Biol_11_412
Author(s) : Harel M , Aharoni A , Gaidukov L , Brumshtein B , Khersonsky O , Meged R , Dvir H , Ravelli RB , McCarthy A , Toker L , Silman I , Sussman JL , Tawfik DS
Ref : Nat Struct Mol Biol , 11 :412 , 2004
PubMedID: 15098021

Title : Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization - Aharoni_2004_Proc.Natl.Acad.Sci.U.S.A_101_482
Author(s) : Aharoni A , Gaidukov L , Yagur S , Toker L , Silman I , Tawfik DS
Ref : Proc Natl Acad Sci U S A , 101 :482 , 2004
PubMedID: 14695884

Title : Directed evolution of recombinant serum paraoxonase (PON) variants - Aharoni_2004_Discov.Med_4_120
Author(s) : Aharoni A , Tawfik DS
Ref : Discov Med , 4 :120 , 2004
PubMedID: 20705007

Title : Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization - Griffiths_2003_EMBO.J_22_24
Author(s) : Griffiths AD , Tawfik DS
Ref : EMBO Journal , 22 :24 , 2003
PubMedID: 12505981

Title : Esterolytic antibodies as mechanistic and structural models of hydrolases-a quantitative analysis - Lindner_2002_J.Mol.Biol_320_559
Author(s) : Lindner AB , Kim SH , Schindler DG , Eshhar Z , Tawfik DS
Ref : Journal of Molecular Biology , 320 :559 , 2002
PubMedID: 12096909

Title : Man-made cell-like compartments for molecular evolution - Tawfik_1998_Nat.Biotechnol_16_652
Author(s) : Tawfik DS , Griffiths AD
Ref : Nat Biotechnol , 16 :652 , 1998
PubMedID: 9661199