L48R_human-ACHE

General

Gene Locus : human-ACHE

Mode of mutation : Site directed mutagenesis

Disease :

Summary : PROSS algorithm\;Expression in E Coli\;Goldenzweig_2016_Mol.Cell_63_337

AAA Change :

Allelic Variant :

Risk Factor :

Inhibitor :

Structure : 5HQ3

Disease by interaction :

Interact Gene Locus :

Xenobiotic sensitivity :

Modification : Expression in E Coli || PROSS algorithm

Torpedo_number : 46

Kinetic Parameter : No kinetic parameter

News : No news

Comment : "p.L48R Leu48Arg (p.L79R Leu79Arg in primary sequence with 31 amino-acids signal peptide) mutation designed by -Protein Repair One Stop Shop- process for the production of active human acetylcholinesterase in a prokaryotic system. The mutation gives Arg as in Torpedo californica and drosophila AChE"

References (1)

Title : Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability - Goldenzweig_2016_Mol.Cell_63_337
Author(s) : Goldenzweig A , Goldsmith M , Hill SE , Gertman O , Laurino P , Ashani Y , Dym O , Unger T , Albeck S , Prilusky J , Lieberman RL , Aharoni A , Silman I , Sussman JL , Tawfik DS , Fleishman SJ
Ref : Mol Cell , 63 :337 , 2016
Abstract : Goldenzweig_2016_Mol.Cell_63_337
ESTHER : Goldenzweig_2016_Mol.Cell_63_337
PubMedSearch : Goldenzweig_2016_Mol.Cell_63_337
PubMedID: 27425410
Gene_locus related to this paper: human-ACHE