| Title : Ric8 acts as a regulator of G-protein signaling required for nematode-trapping lifecycle of Arthrobotrys oligospora - Bai_2021_Environ.Microbiol__ |
| Author(s) : Bai N , Zhang G , Wang W , Feng H , Yang X , Zheng Y , Yang L , Xie M , Zhang KQ , Yang J |
| Ref : Environ Microbiol , : , 2021 |
|
Abstract :
Resistance to inhibitors of cholinesterase 8 (Ric8) is a conserved guanine nucleotide exchange factor that is involved in the regulation of G-protein signaling in filamentous fungi. Here, we characterized an orthologous Ric8 (AoRic8) in Arthrobotrys oligospora by multi-omics analyses. The Aoric8 deletion (deltaAoric8) mutants lost an ability to produce traps essential for nematode predation, accompanied by a marked reduction in cAMP level. Yeast two-hybrid assay revealed that AoRic8 interacted with G-protein subunit Galpha1. Moreover, the mutants were compromised in mycelia growth, conidiation, stress resistance, endocytosis, cellular components, and intrahyphal hyphae. Revealed by transcriptomic analysis differentially upregulated genes in the absence of Aoric8 were involved in cell cycle, DNA replication, and recombination during trap formation while downregulated genes were primarily involved in organelles, carbohydrate metabolism, and amino acid metabolism. Metabolomic analysis showed that many compounds were markedly downregulated in deltaAoric8 mutants versus the wild-type strain. Our results demonstrated a crucial role for AoRic8 in the fungal growth, environmental adaption, and nematode predation through control of cell cycle, organelle, and secondary metabolism by G-protein signaling. This article is protected by copyright. All rights reserved. |
| PubMedSearch : Bai_2021_Environ.Microbiol__ |
| PubMedID: 34431203 |
Bai N, Zhang G, Wang W, Feng H, Yang X, Zheng Y, Yang L, Xie M, Zhang KQ, Yang J (2021)
Ric8 acts as a regulator of G-protein signaling required for nematode-trapping lifecycle of Arthrobotrys oligospora
Environ Microbiol
:
Bai N, Zhang G, Wang W, Feng H, Yang X, Zheng Y, Yang L, Xie M, Zhang KQ, Yang J (2021)
Environ Microbiol
: