Biberoglu_2013_Biochim.Biophys.Acta_1834_745

Reference

Title : Polyproline tetramer organizing peptides in fetal bovine serum acetylcholinesterase - Biberoglu_2013_Biochim.Biophys.Acta_1834_745
Author(s) : Biberoglu K , Schopfer LM , Saxena A , Tacal O , Lockridge O
Ref : Biochimica & Biophysica Acta , 1834 :745 , 2013
Abstract :

Acetylcholinesterase (AChE) in the serum of fetal cow is a tetramer. The related enzyme, butyrylcholinesterase (BChE), in the sera of humans and horse requires polyproline peptides for assembly into tetramers. Our goal was to determine whether soluble tetrameric AChE includes tetramer organizing peptides in its structure. Fetal bovine serum AChE was denatured by boiling to release non-covalently bound peptides. Bulk protein was separated from peptides by filtration and by high performance liquid chromatography. Peptide mass and amino acid sequence of the released peptides were determined by MALDI-TOF-TOF and LTQ-Orbitrap mass spectrometry. Twenty polyproline peptides, divided into 5 families, were identified. The longest peptide contained 25 consecutive prolines and no other amino acid. Other polyproline peptides included one non-proline amino acid, for example serine at the C-terminus of 20 prolines. A search of the mammalian proteome database suggested that this assortment of polyproline peptides originated from at least 5 different precursor proteins, none of which were the ColQ or PRiMA of membrane-anchored AChE. To date, AChE and BChE are the only proteins known that include polyproline tetramer organizing peptides in their tetrameric structure.

PubMedSearch : Biberoglu_2013_Biochim.Biophys.Acta_1834_745
PubMedID: 23352838

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Citations formats

Biberoglu K, Schopfer LM, Saxena A, Tacal O, Lockridge O (2013)
Polyproline tetramer organizing peptides in fetal bovine serum acetylcholinesterase
Biochimica & Biophysica Acta 1834 :745

Biberoglu K, Schopfer LM, Saxena A, Tacal O, Lockridge O (2013)
Biochimica & Biophysica Acta 1834 :745