Capela_2001_Proc.Natl.Acad.Sci.U.S.A_98_9877

Reference

Title : Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021 - Capela_2001_Proc.Natl.Acad.Sci.U.S.A_98_9877
Author(s) : Capela D , Barloy-Hubler F , Gouzy J , Bothe G , Ampe F , Batut J , Boistard P , Becker A , Boutry M , Cadieu E , Dreano S , Gloux S , Godrie T , Goffeau A , Kahn D , Kiss E , Lelaure V , Masuy D , Pohl T , Portetelle D , Puhler A , Purnelle B , Ramsperger U , Renard C , Thebault P , Vandenbol M , Weidner S , Galibert F
Ref : Proc Natl Acad Sci U S A , 98 :9877 , 2001
Abstract :

Sinorhizobium meliloti is an alpha-proteobacterium that forms agronomically important N(2)-fixing root nodules in legumes. We report here the complete sequence of the largest constituent of its genome, a 62.7% GC-rich 3,654,135-bp circular chromosome. Annotation allowed assignment of a function to 59% of the 3,341 predicted protein-coding ORFs, the rest exhibiting partial, weak, or no similarity with any known sequence. Unexpectedly, the level of reiteration within this replicon is low, with only two genes duplicated with more than 90% nucleotide sequence identity, transposon elements accounting for 2.2% of the sequence, and a few hundred short repeated palindromic motifs (RIME1, RIME2, and C) widespread over the chromosome. Three regions with a significantly lower GC content are most likely of external origin. Detailed annotation revealed that this replicon contains all housekeeping genes except two essential genes that are located on pSymB. Amino acid/peptide transport and degradation and sugar metabolism appear as two major features of the S. meliloti chromosome. The presence in this replicon of a large number of nucleotide cyclases with a peculiar structure, as well as of genes homologous to virulence determinants of animal and plant pathogens, opens perspectives in the study of this bacterium both as a free-living soil microorganism and as a plant symbiont.

PubMedSearch : Capela_2001_Proc.Natl.Acad.Sci.U.S.A_98_9877
PubMedID: 11481430
Gene_locus related to this paper: rhime-ACVB , rhime-PIP1 , rhime-PIP2 , rhime-PIP3 , rhime-PLDB , rhime-PTRB , rhime-q92ju9 , rhime-q92ke0 , rhime-q92nd1 , rhime-q92td3 , rhime-R00281 , rhime-R00428 , rhime-R00762 , rhime-R01391 , rhime-R01482 , rhime-R01647 , rhime-R01737 , rhime-R01762 , rhime-R01861 , rhime-R01962 , rhime-R01994 , rhime-R02203 , rhime-R02260 , rhime-R02262 , rhime-R02478 , rhime-R02490 , rhime-R02995 , rhime-R03193 , sinmb-a0a0e0ub90

Related information

Gene_locus rhime-ACVB    rhime-PIP1    rhime-PIP2    rhime-PIP3    rhime-PLDB    rhime-PTRB    rhime-q92ju9    rhime-q92ke0    rhime-q92nd1    rhime-q92td3    rhime-R00281    rhime-R00428    rhime-R00762    rhime-R01391    rhime-R01482    rhime-R01647    rhime-R01737    rhime-R01762    rhime-R01861    rhime-R01962    rhime-R01994    rhime-R02203    rhime-R02260    rhime-R02262    rhime-R02478    rhime-R02490    rhime-R02995    rhime-R03193    sinmb-a0a0e0ub90

Citations formats

Capela D, Barloy-Hubler F, Gouzy J, Bothe G, Ampe F, Batut J, Boistard P, Becker A, Boutry M, Cadieu E, Dreano S, Gloux S, Godrie T, Goffeau A, Kahn D, Kiss E, Lelaure V, Masuy D, Pohl T, Portetelle D, Puhler A, Purnelle B, Ramsperger U, Renard C, Thebault P, Vandenbol M, Weidner S, Galibert F (2001)
Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021
Proc Natl Acad Sci U S A 98 :9877

Capela D, Barloy-Hubler F, Gouzy J, Bothe G, Ampe F, Batut J, Boistard P, Becker A, Boutry M, Cadieu E, Dreano S, Gloux S, Godrie T, Goffeau A, Kahn D, Kiss E, Lelaure V, Masuy D, Pohl T, Portetelle D, Puhler A, Purnelle B, Ramsperger U, Renard C, Thebault P, Vandenbol M, Weidner S, Galibert F (2001)
Proc Natl Acad Sci U S A 98 :9877