rhime-R00281
Rhizobium meliloti (Sinorhizobium meliloti) polyester-degrading carboxylesterase r00281 SM0281
Comment
It has little significant similarity to known PETases (LCC, IsPETase, TfCut1, TfCut2) and to other characterized polyesterases. Degrades BHET, 3PET (BETEB), polycaprolactone (PCL) but not MHET. SM0281 showed slightly higher substrate affinity and catalytic efficiency than IsPETase under similar conditions. MHET does not exert an inhibitory effect on SM0281 activity
Relationship
Family : 6_AlphaBeta_hydrolase
Block : X
Position in NCBI Life Tree : Sinorhizobium meliloti
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Proteobacteria N E > Alphaproteobacteria N E > Rhizobiales N E > Rhizobiaceae N E > Sinorhizobium\/Ensifer group N E > Sinorhizobium N E > Sinorhizobium meliloti N EMolecular evidence
Sequence
Peptide
MSTTPAETEI TRIEVGTGAA ARSIAMRIFR TGNPPDMKDG DPRRPALVWL GGYRSDMTGT KAVEVERHAR EAGTDCIRFD YSGHGASDGD YRDGTISRWV EESLAVIDHA ATGRMILIGS SMGAWVALRL AEKLKGGAHH GEGGVGRLCG LVLIAPAPDF TAELIEPNLT EAERTSLAER GYFEEPSEYS PEPNVFTRAL IEDGRNNLVM KGPIETGCPV HILQGMRDPD VPYTHALKLM EHMPADDVVM TLIRDGDHRL SREEDIAKLK QAIDAMLTKA
References (2)
| Title : Crystal structure and biochemical analysis of the polyester-degrading carboxylesterase SM0281 from the symbiotic legume-associated rhizobacterium Sinorhizobium (Ensifer) meliloti - Lemak_2026_Febs.j__ |
| Author(s) : Lemak S , Khusnutdinova AN , Stogios PJ , Evdokimova E , Savchenko A , Golyshin PN , Edwards EA , Yakunin AF |
| Ref : Febs J , : , 2026 |
| Abstract : |
| PubMedSearch : Lemak_2026_Febs.j__ |
| PubMedID: 42770702 |
| Gene_locus related to this paper: rhime-R00281 |