| Title : Structural basis of cycloaddition in biosynthesis of iboga and aspidosperma alkaloids - Caputi_2020_Nat.Chem.Biol_16_383 |
| Author(s) : Caputi L , Franke J , Bussey K , Farrow SC , Vieira IJC , Stevenson CEM , Lawson DM , O'Connor SE |
| Ref : Nat Chemical Biology , 16 :383 , 2020 |
|
Abstract :
Cycloaddition reactions generate chemical complexity in a single step. Here we report the crystal structures of three homologous plant-derived cyclases involved in the biosynthesis of iboga and aspidosperma alkaloids. These enzymes act on the same substrate, named angryline, to generate three distinct scaffolds. Mutational analysis reveals how these highly similar enzymes control regio- and stereo-selectivity. |
| PubMedSearch : Caputi_2020_Nat.Chem.Biol_16_383 |
| PubMedID: 32066966 |
| Gene_locus related to this paper: catro-CS , catro-TS , tabib-a0a5b8x6s2 , tabib-CorS |
| Inhibitor | 18-carboxymethoxy-cleaviminium |
| Substrate | Dihydroprecondylocarpine-acetate Dehydrosecodine |
| Gene_locus | catro-CS catro-TS tabib-a0a5b8x6s2 tabib-CorS |
| Family | Plant_carboxylesterase |
| Structure | 6RT8 6RJ8 6RS4 |
Caputi L, Franke J, Bussey K, Farrow SC, Vieira IJC, Stevenson CEM, Lawson DM, O'Connor SE (2020)
Structural basis of cycloaddition in biosynthesis of iboga and aspidosperma alkaloids
Nat Chemical Biology
16 :383
Caputi L, Franke J, Bussey K, Farrow SC, Vieira IJC, Stevenson CEM, Lawson DM, O'Connor SE (2020)
Nat Chemical Biology
16 :383