Greenblatt_2012_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_F68_269

Reference

Title : Structure of Recombinant Human Carboxylesterase 1 Isolated from Whole Cabbage Looper Larvae - Greenblatt_2012_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_F68_269
Author(s) : Greenblatt HM , Otto TC , Kirkpatrick MG , Kovaleva E , Brown S , Buchman G , Cerasoli DM , Sussman JL
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , F68 :269 , 2012
Abstract : The use of whole insect larvae as a source of recombinant proteins offers a more cost-effective method of producing large quantities of human proteins than conventional cell-culture approaches. Human carboxylesterase 1 has been produced in and isolated from whole Trichoplusia ni larvae. The recombinant protein was crystallized and its structure was solved to 2.2 resolution. The results indicate that the larvae-produced enzyme is essentially identical to that isolated from cultured Sf21 cells, supporting the use of this expression system to produce recombinant enzymes for crystallization studies.
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PubMedSearch : Greenblatt_2012_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_F68_269
PubMedID: 22442219
Gene_locus related to this paper: human-CES1

Related information

Gene_locus related to this paper: human-CES1

Citations formats

Greenblatt HM, Otto TC, Kirkpatrick MG, Kovaleva E, Brown S, Buchman G, Cerasoli DM, Sussman JL (2012)
Structure of Recombinant Human Carboxylesterase 1 Isolated from Whole Cabbage Looper Larvae
Acta Crystallographica Sect F Struct Biol Cryst Commun F68 :269

Greenblatt HM, Otto TC, Kirkpatrick MG, Kovaleva E, Brown S, Buchman G, Cerasoli DM, Sussman JL (2012)
Acta Crystallographica Sect F Struct Biol Cryst Commun F68 :269