Joo_2018_Nat.Commun_9_382

Reference

Title : Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation - Joo_2018_Nat.Commun_9_382
Author(s) : Joo S , Cho IJ , Seo H , Son HF , Sagong HY , Shin TJ , Choi SY , Lee SY , Kim KJ
Ref : Nat Commun , 9 :382 , 2018
Abstract :

Plastics, including poly(ethylene terephthalate) (PET), possess many desirable characteristics and thus are widely used in daily life. However, non-biodegradability, once thought to be an advantage offered by plastics, is causing major environmental problem. Recently, a PET-degrading bacterium, Ideonella sakaiensis, was identified and suggested for possible use in degradation and/or recycling of PET. However, the molecular mechanism of PET degradation is not known. Here we report the crystal structure of I. sakaiensis PETase (IsPETase) at 1.5 A resolution. IsPETase has a Ser-His-Asp catalytic triad at its active site and contains an optimal substrate binding site to accommodate four monohydroxyethyl terephthalate (MHET) moieties of PET. Based on structural and site-directed mutagenesis experiments, the detailed process of PET degradation into MHET, terephthalic acid, and ethylene glycol is suggested. Moreover, other PETase candidates potentially having high PET-degrading activities are suggested based on phylogenetic tree analysis of 69 PETase-like proteins.

PubMedSearch : Joo_2018_Nat.Commun_9_382
PubMedID: 29374183
Gene_locus related to this paper: idesa-peth

Related information

Inhibitor Terephthalic-acid    MHET
Substrate 2HE(MHET)4    2HE(MHET)2    HEMT    MHET    BHET    Polyethylene-terephthalate
Gene_locus idesa-peth
Family Polyesterase-lipase-cutinase
Structure 5YNS    5XJH
Chemical Ethylene-glycol    Terephthalic-acid

Citations formats

Joo S, Cho IJ, Seo H, Son HF, Sagong HY, Shin TJ, Choi SY, Lee SY, Kim KJ (2018)
Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation
Nat Commun 9 :382

Joo S, Cho IJ, Seo H, Son HF, Sagong HY, Shin TJ, Choi SY, Lee SY, Kim KJ (2018)
Nat Commun 9 :382