| Title : Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta - Kanost_2016_Insect.Biochem.Mol.Biol_76_118 |
| Author(s) : Kanost MR , Arrese EL , Cao X , Chen YR , Chellapilla S , Goldsmith MR , Grosse-Wilde E , Heckel DG , Herndon N , Jiang H , Papanicolaou A , Qu J , Soulages JL , Vogel H , Walters J , Waterhouse RM , Ahn SJ , Almeida FC , An C , Aqrawi P , Bretschneider A , Bryant WB , Bucks S , Chao H , Chevignon G , Christen JM , Clarke DF , Dittmer NT , Ferguson LCF , Garavelou S , Gordon KHJ , Gunaratna RT , Han Y , Hauser F , He Y , Heidel-Fischer H , Hirsh A , Hu Y , Kalra D , Klinner C , Konig C , Kovar C , Kroll AR , Kuwar SS , Lee SL , Lehman R , Li K , Li Z , Liang H , Lovelace S , Lu Z , Mansfield JH , McCulloch KJ , Mathew T , Morton B , Muzny DM , Neunemann D , Ongeri F , Pauchet Y , Pu LL , Pyrousis I , Rao XJ , Redding A , Roesel C , Sanchez-Gracia A , Schaack S , Shukla A , Tetreau G , Wang Y , Xiong GH , Traut W , Walsh TK , Worley KC , Wu D , Wu W , Wu YQ , Zhang X , Zou Z , Zucker H , Briscoe AD , Burmester T , Clem RJ , Feyereisen R , Grimmelikhuijzen CJP , Hamodrakas SJ , Hansson BS , Huguet E , Jermiin LS , Lan Q , Lehman HK , Lorenzen M , Merzendorfer H , Michalopoulos I , Morton DB , Muthukrishnan S , Oakeshott JG , Palmer W , Park Y , Passarelli AL , Rozas J , Schwartz LM , Smith W , Southgate A , Vilcinskas A , Vogt R , Wang P , Werren J , Yu XQ , Zhou JJ , Brown SJ , Scherer SE , Richards S , Blissard GW |
| Ref : Insect Biochemistry & Molecular Biology , 76 :118 , 2016 |
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Abstract :
Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects. |
| PubMedSearch : Kanost_2016_Insect.Biochem.Mol.Biol_76_118 |
| PubMedID: 27522922 |
| Gene_locus related to this paper: manse-a0a921zfz9 |
| Gene_locus | manse-a0a921zfz9 |
Kanost MR, Arrese EL, Cao X, Chen YR, Chellapilla S, Goldsmith MR, Grosse-Wilde E, Heckel DG, Herndon N, Jiang H, Papanicolaou A, Qu J, Soulages JL, Vogel H, Walters J, Waterhouse RM, Ahn SJ, Almeida FC, An C, Aqrawi P, Bretschneider A, Bryant WB, Bucks S, Chao H, Chevignon G, Christen JM, Clarke DF, Dittmer NT, Ferguson LCF, Garavelou S, Gordon KHJ, Gunaratna RT, Han Y, Hauser F, He Y, Heidel-Fischer H, Hirsh A, Hu Y, Kalra D, Klinner C, Konig C, Kovar C, Kroll AR, Kuwar SS, Lee SL, Lehman R, Li K, Li Z, Liang H, Lovelace S, Lu Z, Mansfield JH, McCulloch KJ, Mathew T, Morton B, Muzny DM, Neunemann D, Ongeri F, Pauchet Y, Pu LL, Pyrousis I, Rao XJ, Redding A, Roesel C, Sanchez-Gracia A, Schaack S, Shukla A, Tetreau G, Wang Y, Xiong GH, Traut W, Walsh TK, Worley KC, Wu D, Wu W, Wu YQ, Zhang X, Zou Z, Zucker H, Briscoe AD, Burmester T, Clem RJ, Feyereisen R, Grimmelikhuijzen CJP, Hamodrakas SJ, Hansson BS, Huguet E, Jermiin LS, Lan Q, Lehman HK, Lorenzen M, Merzendorfer H, Michalopoulos I, Morton DB, Muthukrishnan S, Oakeshott JG, Palmer W, Park Y, Passarelli AL, Rozas J, Schwartz LM, Smith W, Southgate A, Vilcinskas A, Vogt R, Wang P, Werren J, Yu XQ, Zhou JJ, Brown SJ, Scherer SE, Richards S, Blissard GW (2016)
Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta
Insect Biochemistry & Molecular Biology
76 :118
Kanost MR, Arrese EL, Cao X, Chen YR, Chellapilla S, Goldsmith MR, Grosse-Wilde E, Heckel DG, Herndon N, Jiang H, Papanicolaou A, Qu J, Soulages JL, Vogel H, Walters J, Waterhouse RM, Ahn SJ, Almeida FC, An C, Aqrawi P, Bretschneider A, Bryant WB, Bucks S, Chao H, Chevignon G, Christen JM, Clarke DF, Dittmer NT, Ferguson LCF, Garavelou S, Gordon KHJ, Gunaratna RT, Han Y, Hauser F, He Y, Heidel-Fischer H, Hirsh A, Hu Y, Kalra D, Klinner C, Konig C, Kovar C, Kroll AR, Kuwar SS, Lee SL, Lehman R, Li K, Li Z, Liang H, Lovelace S, Lu Z, Mansfield JH, McCulloch KJ, Mathew T, Morton B, Muzny DM, Neunemann D, Ongeri F, Pauchet Y, Pu LL, Pyrousis I, Rao XJ, Redding A, Roesel C, Sanchez-Gracia A, Schaack S, Shukla A, Tetreau G, Wang Y, Xiong GH, Traut W, Walsh TK, Worley KC, Wu D, Wu W, Wu YQ, Zhang X, Zou Z, Zucker H, Briscoe AD, Burmester T, Clem RJ, Feyereisen R, Grimmelikhuijzen CJP, Hamodrakas SJ, Hansson BS, Huguet E, Jermiin LS, Lan Q, Lehman HK, Lorenzen M, Merzendorfer H, Michalopoulos I, Morton DB, Muthukrishnan S, Oakeshott JG, Palmer W, Park Y, Passarelli AL, Rozas J, Schwartz LM, Smith W, Southgate A, Vilcinskas A, Vogt R, Wang P, Werren J, Yu XQ, Zhou JJ, Brown SJ, Scherer SE, Richards S, Blissard GW (2016)
Insect Biochemistry & Molecular Biology
76 :118