Duf_1100-S
Relationship
Comment
DUF1100 UPF0255. The family was split in two. Duf_1100-S with active site (S217 in (GXSXG) D300 H329) as in artni-Q93NG6, and Duf_1100-R with active site (R53, D203, R 272 (in GXRXG)) as in FrsA. This family is represented by a C-C bond cleaving alpha/beta-hydrolase involved in nicotine degradation: 2,6-dihydroxy-pseudo-oxynicotine hydrolase from the nicotine-degradation pathway of Arthrobacter nicotinovorans (artni-Q93NG6a) (Schleberger et al.). The family also includes the hydrolytic polyketide shortening protein Ayg1 from fungi (Fujii et al.), and Fus2 from Gibberella species, which is part of the gene cluster that mediates the biosynthesis of the mycotoxin fusarin C. Fus2 catalyses closure of the 2-pyrrolidone ring of the intermediate 20-hydroxy-prefusarin to form another intermediate, 20-hydroxy-fusarin, which is then oxidized by Fus8 (Niehaus et al.). Other members of this family are still uncharacterised
Database
Interpro : IPR010520 Esterase FrsA-like Protein of unknown function DUF1100 UPF0255, hydrolase-like
PIRSF : PIRSF004493 YafA protein
Pdoc : No Pdoc
Pfam : PF06500 FrsA-like DUF1100
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 8QD1 | Ayg1p from A. fumigatus catalyzes polyketide shortening in the biosynthesis of DHN-melanin | aspfu-AYG1 |
| 8QD2 | Ayg1p in complex with 1,3-Dihydroxynaphthalene | aspfu-AYG1 |
| 8QD3 | Ayg1p in complex with 1,3,6,8-Tetrahydroxynaphthalene | aspfu-AYG1 |
| 8QD4 | Ayg1p active site converted to tetrahedral sulfonate ester | aspfu-AYG1 |
| 8QBH | AntI from P. luminescens catalyses terminal polyketide shortening in the biosynthesis of anthraquinones AntI in complex with 1-Naphthol | pholl-AntI |
| 8QBI | AntI from P. luminescens catalyses terminal polyketide shortening in the biosynthesis of anthraquinones AntI in closed state | pholl-AntI |
| 8QD5 | AntI from P. luminescens catalyses terminal polyketide shortening in the biosynthesis of anthraquinones AntI Ser245DHA (PSF) | pholl-AntI |
| 8QD6 | AntI from P. luminescens catalyses terminal polyketide shortening in the biosynthesis of anthraquinones AntI Ser245DHA (PMSF) | pholl-AntI |
| 8QD7 | Wdyg1p from W. dermatitidis catalyzes polyketide shortening in the biosynthesis of DHN-melanin | exode-q6bcb5 |
| 8QD8 | Wdyg1p in complex with 1,3-Dihydroxynaphthalene | exode-q6bcb5 |
| 8QD9 | Wdyg1p in complex with 1,3,6,8-Tetrahydroxynaphthalene | exode-q6bcb5 |
| 8QDA | Wdyg1p Ser256DHA (PMSF) | exode-q6bcb5 |
| 8QDB | Wdyg1p Ser256DHA (PSF) | exode-q6bcb5 |
| 6HXA | AntI from P. luminescens catalyses terminal polyketide shortening in the biosynthesis of anthraquinones | pholl-AntI |
| 5NO5 | Structure of Verrucosispora maris AbyA5 Wildtype | verma-f4f7f5 |
| 4YWF | Structure of Verrucosispora maris AbyA5 at 2.0 Angstroms | verma-f4f7f5 |
| 3FNB | Crystal structure of acylaminoacyl-peptidase SMU_737 from Streptococcus mutans UA159 | strmu-SMU.737 |
| 2JBW | Crystal structure of the 2,6-Dihydroxy-pseudo-oxynicotine hydrolase | artni-Q93NG6 |
| Gene_locus | Name | Species |
|---|---|---|
| metra-e9fcw4 | Metarhizium robertsii ARSEF 23 ccsE Alpha\/beta hydrolase | Metarhizium robertsii ARSEF 23 |
| phano-phmG | Phaeosphaeria nodorum (strain SN15 \/ ATCC MYA-4574 \/ FGSC 10173) (Glume blotch fungus) (Parastagonospora nodorum) Hydrolase phmG | Parastagonospora nodorum |
| aspcl-CCSE | Aspergillus clavatus (strain ATCC 1007 \/ CBS 513.65 \/ DSM 816 \/ NCTC 3887 \/ NRRL 1) Hydrolyase ccsE | Aspergillus clavatus |
| aspfu-psoB | Neosartorya fumigata (Aspergillus fumigatus) Alpha\/beta hydrolase psoB | Aspergillus fumigatus Af293 |
| mago7-ORFZB | Magnaporthe grisea (Crabgrass-specific blast fungus) (Pyricularia grisea) Hydrolase ORFZ | Magnaporthe grisea |
| maggr-pyie | Magnaporthe grisea (Crabgrass-specific blast fungus) (Pyricularia grisea) Hydrolase pyiE | Magnaporthe grisea |
| pholl-AntI |  Photorhabdus luminescens (subsp. laumondii)(strain DSM 15139 \/ CIP 105565 \/ TT01) plu4186 AntI polyketide shortening enzyme |
Photorhabdus luminescens |
| gibf5-fus2 | Gibberella fujikuroi (Bakanae and foot rot disease fungus) (Fusarium fujikuroi)\; Gibberella moniliformis (Maize ear and stalk rot fungus) (Fusarium verticillioides) 20-hydroxy-prefusarin hydrolase FUS2 | Gibberella fujikuroi |
| verma-f4f7f5 | Verrucosispora maris (strain AB-18-032) AbyA5 |
Verrucosispora maris (strain AB-18-032) |
| 9acto-q1mx66 | Streptomyces sp. NRRL 11266 putative hydrolase or acyltransferase | Streptomyces sp. NRRL 11266 |
| nocsj-A1SEB2 | Nocardioides sp. JS614 hypothetical protein | Nocardioides sp. JS614 |
| nocsj-A1SQ88 | Nocardioides sp. JS614 probable dipeptidyl aminopeptidase | Nocardioides sp. JS614 |
| 9arch-d1jaz1 | uncultured archaeon Putative uncharacterized protein | uncultured archaeon |
| 9baci-q2azx1 | Bacillus weihenstephanensis KBAB4 probable dipeptidyl aminopeptidase | Bacillus weihenstephanensis KBAB4 |
| 9chlr-d6tbj6 | Ktedonobacter racemifer DSM 44963 BAAT\/Acyl-CoA thioester hydrolase | Ktedonobacter racemifer DSM 44963 |
| 9clot-a8rn00 | Clostridium bolteae ATCC BAA-613 Putative uncharacterized protein | Clostridium bolteae ATCC BAA-613 |
| 9clot-c6pte9 | Clostridium carboxidivorans P7 Putative uncharacterized protein | Clostridium carboxidivorans P7 |
| 9firm-d2kfp1 | Cellulosilyticum ruminicola Esterase | Cellulosilyticum ruminicola |
| 9laco-c2ent8 | Lactobacillus ultunensis DSM 16047 Dipeptidyl aminopeptidase\/acylaminoacyl peptidase | Lactobacillus ultunensis DSM 16047 |
| 9laco-c8p8a9 | Lactobacillus antri DSM 16041 Putative uncharacterized protein | Lactobacillus antri DSM 16041 |
| 9rhob-a3vdm3 | Maritimibacter alkaliphilus HTCC2654 Putative uncharacterized protein | Maritimibacter alkaliphilus HTCC2654 |
| acice-q2e207 | Acidothermus cellulolyticus 11B dipeptidyl aminopeptidases\/acylaminoacyl-peptidases-like | Acidothermus cellulolyticus 11B |
| artni-Q93NG6 | Arthrobacter nicotinovorans 2,6-dihydropseudooxynicotine hydrolase DHPON hydrolase |
Arthrobacter nicotinovorans |
| aspfu-AYG1 | Aspergillus fumigatus (Sartorya fumigata) yellowish-green 1.Heptaketide hydrolyase ayg1 |
Aspergillus fumigatus |
| aspnc-a2r3s8 | Aspergillus niger (strain CBS 513.88 \/ FGSC A1513) Function: ayg1 of A. fumigatus has an unknown function | Aspergillus niger |
| aspor-q2uab6 | Aspergillus oryzae\; Aspergillus flavus predicted hydrolases or acyltransferases | Aspergillus oryzae |
| aspor-q2usq8 | Aspergillus oryzae predicted protein | Aspergillus oryzae |
| bacha-BH1839 | Bacillus halodurans hypothetical protein bh1839 | Bacillus halodurans |
| bactu-c3ice0 | Bacillus thuringiensis, Bacillus weihenstephanensis, Bacillus cereus Putative uncharacterized protein | Bacillus thuringiensis IBL 200 |
| braja-BLL4985 | Bradyrhizobium japonicum (strain USDA 110) bll4985 protein | Bradyrhizobium japonicum |
| braja-BLL6428 | Bradyrhizobium japonicum bll6428 protein | Bradyrhizobium japonicum |
| braja-BLL6890 | Bradyrhizobium japonicum bll6890 protein | Bradyrhizobium japonicum |
| burs3-q397l9 | Burkholderia lata hypothetical protein | Burkholderia sp. |
| chagb-q2h3x4 | Chaetomium globosum CBS 148.51 hypothetical protein | Chaetomium globosum CBS 148.51 |
| chrvo-q7nzq6 | Chromobacterium violaceum probable dipeptidyl aminopeptidase | Chromobacterium violaceum |
| cloac-CAC3402 | Clostridium acetobutylicum Dipeptidyl aminopeptidase\/acylaminoacyl-peptidase related protein | Clostridium acetobutylicum |
| cloac-CAC3515 | Clostridium acetobutylicum alpha\/beta superfamily hydrolase (possible peptidase) | Clostridium acetobutylicum |
| cocps-e9dbi4 | Coccidioides posadasii (strains RMSCC 757 \/ Silveira\; C735) (Valley fever fungus) Putative uncharacterized protein | Coccidioides posadasii |
| colgm-e3qun0 | Colletotrichum graminicola (strain M1.001 \/ M2 \/ FGSC 10212) (Maize anthracnose fungus) (Glomerella graminicola) Alpha\/beta hydrolase | Colletotrichum graminicola |
| conwi-d3f4h1 | Conexibacter woesei (strain DSM 14684 \/ JCM 11494 \/ NBRC 100937 \/ ID131577) Putative uncharacterized protein | Conexibacter woesei |
| cuppj-q46mv6 | Cupriavidus pinatubonensis (strain JMP134 \/ LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) acetyl xylan esterase | Ralstonia eutropha |
| desao-e6vtg3 | Desulfovibrio aespoeensis (strain ATCC 700646 \/ DSM 10631 \/ Aspo-2) Putative uncharacterized protein | Desulfovibrio aespoeensis |
| desps-q6akv7 | Desulfotalea psychrophila hypothetical protein | Desulfotalea psychrophila |
| dicd5-d2c1r0 | Dickeya dadantii (strain Ech586) Putative uncharacterized protein | Dickeya dadantii |
| emeni-q5ara9 | Aspergillus nidulans FGSC A4 Emericella nidulans (strain FGSC A4 \/ ATCC 38163 \/ CBS 112.46 \/ NRR 194 \/ M139) (Aspergillus nidulans) hypothetical protein | Emericella nidulans |
| exode-q6bcb5 | Exophiala dermatitidis (Wangiella dermatitidis) yellowish-green 1-like protein Wdyg1p |
Exophiala dermatitidis |
| grocl-f0xbs9 | Grosmannia clavigera (strain kw1407 \/ UAMH 11150) (Blue stain fungus) (Graphiocladiella clavigera) Pigment biosynthesis protein yellowish-green 1 | Grosmannia clavigera |
| lacga-q043m1 | Lactobacillus gasseri (strains ATCC 33323 \/ DSM 20243\; 202-4\; 224-1\; MV-22) Alpha\/beta hydrolase superfamily enzyme | Lactobacillus gasseri |
| metac-MA0362 | Methanosarcina acetivorans hypothetical protein ma0362 | Methanosarcina acetivorans |
| metac-MA2002 | Methanosarcina acetivorans hypothetical protein ma2002 | Methanosarcina acetivorans |
| metar-e9eqh0 | Metarhizium robertsii (strain ARSEF 23 \/ ATCC MYA-3075) (Metarhizium anisopliae) Dipeptidyl aminopeptidase\/acylaminoacyl peptidase | Metarhizium robertsii |
| micch-b5l6l6 | Micromonospora chalcea DUF1100 | Micromonospora chalcea |
| mycpa-q73tg1 | Mycobacterium paratuberculosis hypothetical protein | Mycobacterium paratuberculosis |
| neucr-q7s5m2 | Neurospora crassa hypothetical protein | Neurospora crassa |
| neucr-q7sha3 | Neurospora crassa hypothetical protein | Neurospora crassa |
| nosp7-b2j516 | Nostoc punctiforme (strain ATCC 29133 \/ PCC 73102) Putative uncharacterized protein | Nostoc punctiforme |
| nosp7-b2j537 | Nostoc punctiforme (strain ATCC 29133 \/ PCC 73102) Putative uncharacterized protein | Nostoc punctiforme |
| novad-q2gag2 | Novosphingobium aromaticivorans (strain DSM 12444) hypothetical protein | Novosphingobium aromaticivorans |
| parde-q3pfk3 | Paracoccus denitrificans PD1222 hypothetical protein precursor | Paracoccus denitrificans PD1222 |
| pencw-b6hat0 | Penicillium chrysogenum (strain ATCC 28089 \/ DSM 1075 \/ Wisconsin 54-1255) (Penicillium notatum) Pc16g13900 protein | Penicillium chrysogenum |
| pencw-b6hl02 | Penicillium chrysogenum (strain ATCC 28089 \/ DSM 1075 \/ Wisconsin 54-1255) (Penicillium notatum) Pc21g16440 protein | Penicillium chrysogenum |
| phano-q0u753 | Phaeosphaeria nodorum (strain SN15 \/ ATCC MYA-4574 \/ FGSC 10173) (Glume blotch fungus) (Septoria nodorum) Putative uncharacterized protein | Phaeosphaeria nodorum |
| psepu-PP3195 | Pseudomonas putida (strain KT2440) conserved hypothetical protein | Pseudomonas putida |
| pyrtr-b2w948 | Pyrenophora tritici-repentis (Wheat tan spot fungus) (Drechslera tritici-repentis) P. teres f. teres (Barley net blotch fungus) (D. teres f. teres) Pigment biosynthesis protein Ayg1 | Pyrenophora tritici-repentis |
| rhopa-q6nb93 | Rhodopseudomonas palustris hypothetical protein precursor | Rhodopseudomonas palustris |
| spore-e7a048 | Sporisorium reilianum (strain SRZ2) (Maize head smut fungus) Related to yellowish-green 1 (Ayg1) | Sporisorium reilianum |
| strhy-ORFZ | Streptomyces hygroscopicus orfz protein | Streptomyces hygroscopicus |
| strmu-SMU.737 | Streptococcus mutans conserved hypothetical protein |
Streptococcus mutans |
| sulnb-a6q8n3 | Sulfurovum sp. (strain NBC37-1) Putative uncharacterized protein | Sulfurovum sp. NBC37-1 |
| sulnb-a6q8n4 | Sulfurovum sp. (strain NBC37-1) Putative uncharacterized protein | Sulfurovum sp. NBC37-1 |
| talsn-b8mbm1 | Talaromyces stipitatus (strain ATCC 10500 \/ CBS 375.48 \/ QM 6759 \/ NRRL 1006) (Penicillium stipitatum) Conidial pigment biosynthesis protein Ayg1 | Talaromyces stipitatus |
| tertt-c5bkb0 | Teredinibacter turnerae (strain ATCC 39867 \/ T7901) Putative uncharacterized protein | Teredinibacter turnerae |
| ustma-q4pcx8 | Ustilago maydis (strain 521 \/ FGSC 9021) (Corn smut fungus) hypothetical protein | Ustilago maydis 521 |
| vera1-c9sbu9 | Verticillium albo-atrum (strain VaMs.102 \/ ATCC MYA-4576 \/ FGSC 10136) (Verticillium wilt) Pigment biosynthesis protein Ayg1 | Verticillium albo-atrum |
| aspfv-ffse | Aspergillus flavipes. Cytochalasans biosynthesis cluster protein ffsE | Aspergillus flavipes |
| fussx-luc6 | Fusarium sp. Lucilactaene biosynthesis cluster protein 6 | Fusarium sp. |
| penox-poxo | Penicillium oxalicum. Oxaleimides biosynthesis cluster protein O | Penicillium oxalicum |
| acrsp-ucsc | Acremonium sp. UCS1025A pyrrolizidinone biosynthesis cluster protein C | Acremonium sp. |
| aspa1-acrc | Aspergillus aculeatus (strain ATCC 16872 \/ CBS 172.66 \/ WB 5094). Acurin A biosynthesis cluster protein C | Aspergillus aculeatus |
| Gene_locus_frgt | Name | Species |
|---|---|---|
| 9arch-q64bd3 | uncultured archaeon GZfos27E6 Predicted hydrolases or acyltransferases | uncultured archaeon GZfos27E6 |
| uncre-c4jlv8 | Uncinocarpus reesii (strain UAMH 1704) Predicted protein | Uncinocarpus reesii |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| ORFZ-Substrate | mago7-ORFZB maggr-pyie | |
| PyiE-Substrate | mago7-ORFZB maggr-pyie | |
| O-Demethylfonsecin | 2,5,6,8-tetrahydroxy-2-methyl-3H-benzo[g]chromen-4-one | aspfu-AYG1 |
| 6-Hydroxypseudooxynicotine | 5-[4-(methylamino)butanoyl]-1H-pyridin-2-one | artni-Q93NG6 |
| Paranitrophenylbutyrate | (4-nitrophenyl) butanoate | bactc-lipas 9acto-f7ix06 artox-phhyd burgl-EstC bovin-balip rabit-1cxes psesp-OPH 9bact-H6BDX1 9bact-a0a2s1gux0 human-NOTUM 9delt-EstD11 9pezi-s4vch4 aspfu-q4x1n0 crysp-Q874E9 fusso-cutas pyrbr-Q9Y7G8 copci-b9u443 strsw-c9zcr8 humin-cut human-DAGLA 9baci-EstD9 9rhiz-g9i933 canar-LipB bacsu-pnbae thefu-q6a0i4 geost-est30 geost-est50 pyrab-PAB1050 |
| Inhibitor | Chemical Nomenclature | Proteins |
|---|---|---|
| 1-Naphtol | naphthalen-1-ol | pholl-AntI |
| 1,3-Dihydroxynaphthalene | naphthalene-1,3-diol | aspfu-AYG1 |
| 1,3,6,8-Tetrahydroxynaphthalene | naphthalene-1,3,6,8-tetrol | aspfu-AYG1 |
| PMSF | Phenylmethylsulfonyl fluoride | theet-q3ch51 emeni-q5avy9 human-ABHD10 human-PREPL myctu-Rv1430 psefl-cxest 9bact-E40 thefu-q6a0i4 ecoli-Aes orysj-Q10QA5 bac25-mglp 9bact-Q0GMU2 bacsu-FENB psepu-clcd1 thema-TM0077 bacsu-RsbQ ecoli-bioh drome-CG9542 human-FAP tubmm-TmEst2 human-NOTUM cutac-e6dae5 9gamm-urc15994 9baci-EstD9 geost-est30 pholl-AntI |
References (6)
| Title : Computational, structural, and kinetic evidence that Vibrio vulnificus FrsA is not a cofactor-independent pyruvate decarboxylase - Kellett_2013_Biochemistry_52_1842 |
| Author(s) : Kellett WF , Brunk E , Desai BJ , Fedorov AA , Almo SC , Gerlt JA , Rothlisberger U , Richards NG |
| Ref : Biochemistry , 52 :1842 , 2013 |
| Abstract : |
| PubMedSearch : Kellett_2013_Biochemistry_52_1842 |
| PubMedID: 23452154 |
| Gene_locus related to this paper: vibvy-y856 |
| Title : Genetic manipulation of the Fusarium fujikuroi fusarin gene cluster yields insight into the complex regulation and fusarin biosynthetic pathway - Niehaus_2013_Chem.Biol_20_1055 |
| Author(s) : Niehaus EM , Kleigrewe K , Wiemann P , Studt L , Sieber CM , Connolly LR , Freitag M , Guldener U , Tudzynski B , Humpf HU |
| Ref : Chemical Biology , 20 :1055 , 2013 |
| Abstract : |
| PubMedSearch : Niehaus_2013_Chem.Biol_20_1055 |
| PubMedID: 23932525 |
| Gene_locus related to this paper: gibf5-fus2 , gibf5-fus5 |
| Title : FrsA functions as a cofactor-independent decarboxylase to control metabolic flux - Lee_2011_Nat.Chem.Biol_7_434 |
| Author(s) : Lee KJ , Jeong CS , An YJ , Lee HJ , Park SJ , Seok YJ , Kim P , Lee JH , Lee KH , Cha SS |
| Ref : Nat Chemical Biology , 7 :434 , 2011 |
| Abstract : |
| PubMedSearch : Lee_2011_Nat.Chem.Biol_7_434 |
| PubMedID: 21623357 |
| Gene_locus related to this paper: vibvy-y856 |
| Title : Structure and action of a C-C bond cleaving alpha\/beta-hydrolase involved in nicotine degradation - Schleberger_2007_J.Mol.Biol_367_409 |
| Author(s) : Schleberger C , Sachelaru P , Brandsch R , Schulz GE |
| Ref : Journal of Molecular Biology , 367 :409 , 2007 |
| Abstract : |
| PubMedSearch : Schleberger_2007_J.Mol.Biol_367_409 |
| PubMedID: 17275835 |
| Gene_locus related to this paper: artni-Q93NG6 |
| Title : Hydrolytic polyketide shortening by ayg1p, a novel enzyme involved in fungal melanin biosynthesis - Fujii_2004_J.Biol.Chem_279_44613 |
| Author(s) : Fujii I , Yasuoka Y , Tsai HF , Chang YC , Kwon-Chung KJ , Ebizuka Y |
| Ref : Journal of Biological Chemistry , 279 :44613 , 2004 |
| Abstract : |
| PubMedSearch : Fujii_2004_J.Biol.Chem_279_44613 |
| PubMedID: 15310761 |
| Gene_locus related to this paper: aspfu-AYG1 |
| Title : A novel fermentation\/respiration switch protein regulated by enzyme IIAGlc in Escherichia coli - Koo_2004_J.Biol.Chem_279_31613 |
| Author(s) : Koo BM , Yoon MJ , Lee CR , Nam TW , Choe YJ , Jaffe H , Peterkofsky A , Seok YJ |
| Ref : Journal of Biological Chemistry , 279 :31613 , 2004 |
| Abstract : |
| PubMedSearch : Koo_2004_J.Biol.Chem_279_31613 |
| PubMedID: 15169777 |
| Gene_locus related to this paper: vibvy-y856 |